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The map label for this gene is mutM

Identifier: 158335652

GI number: 158335652

Start: 2527272

End: 2528126

Strand: Reverse

Name: mutM

Synonym: AM1_2501

Alternate gene names: 158335652

Gene position: 2528126-2527272 (Counterclockwise)

Preceding gene: 158335653

Following gene: 158335650

Centisome position: 38.87

GC content: 50.18

Gene sequence:

>855_bases
ATGCCTGAGCTACCTGAAGTAGAAACAGTTCGGCTGGGCTTAGAAAAGGTGACTGTTGGGATGCAAATTATGGGTGGCGA
AGTTCTCTATCCACGAACGATTGCTCATCCTCAATCGCCACAAGTATTTATTCAAGGGTTGCAAGATGCAACCTTTTTAT
CATGGATGCGACGTGGAAAATATTTACTGAGTCAGCTTTCTTTCTCTACTCAGCAGCCGTCGGGATGGCTGGGTGTTCAC
CTACGCATGACAGGGCAATTGCTCTGGGTGGCGCAAGATGAGCCCGTCCAGAAACATACTCGAGTGCGTCTATTTTTTGT
GAATAACCGCGAGCTCCGATTTGTAGATCAGCGGACCTTTGGCCAAATGTGGTGGGTGGCTCCGACGGAAGATCCAAAAC
AAGTGATTAGTGGTCTACAGAAGTTGGGACCCGAGCCATTCTCTGAAGAGTTTTCTGTAGACTATTTTTGGGAATCGTTA
CAGGGGCGCAAGCGTTCCATTAAAAGCGCCCTGTTAGATCAGGCTTTGGTGGCAGGAGTCGGCAATATCTATGCAGATGA
AGCCTTATTTATGAGTGAGATTCGCCCCACGACAGCGTGCCATCAGCTGCAGACGGAACAGGTGCAGCGACTACGAACGG
CGATTATTGAGGTTTTGTCGACCAGTATTGGCGCGGGTGGGACCACCTTCAGCGACTTTCGAGATCTCAAAGGGGTGAAC
GGGAATTATGGCGGTATGGCTTGGGTCTATGGACGCCAAGGTCAACCCTGTCGAACCTGTGGTCAAACCATTGAGCGGAT
CAAACTCGTTGGCCGTTCCACCCATTTTTGCCCTCAATGCCAACCGGAATCCTAG

Upstream 100 bases:

>100_bases
CCAAAGGGGGTAAGAAACCTGCTGCTAAGCCTGCTGCAGCGCCTAAGGCAGAAGGCTAACTTACCTTCGTGTCTACTCTT
CGTCCCAACAGTCTGTTTTA

Downstream 100 bases:

>100_bases
AGAATTTCGCGGCTAGCTCTATATAAGTCACTCCAGTTGTCTCGCTCTTTGACTACAGTTTCCAGATATTCTTTCCAGAC
CACTTGATCTTGAACGGGAT

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MPELPEVETVRLGLEKVTVGMQIMGGEVLYPRTIAHPQSPQVFIQGLQDATFLSWMRRGKYLLSQLSFSTQQPSGWLGVH
LRMTGQLLWVAQDEPVQKHTRVRLFFVNNRELRFVDQRTFGQMWWVAPTEDPKQVISGLQKLGPEPFSEEFSVDYFWESL
QGRKRSIKSALLDQALVAGVGNIYADEALFMSEIRPTTACHQLQTEQVQRLRTAIIEVLSTSIGAGGTTFSDFRDLKGVN
GNYGGMAWVYGRQGQPCRTCGQTIERIKLVGRSTHFCPQCQPES

Sequences:

>Translated_284_residues
MPELPEVETVRLGLEKVTVGMQIMGGEVLYPRTIAHPQSPQVFIQGLQDATFLSWMRRGKYLLSQLSFSTQQPSGWLGVH
LRMTGQLLWVAQDEPVQKHTRVRLFFVNNRELRFVDQRTFGQMWWVAPTEDPKQVISGLQKLGPEPFSEEFSVDYFWESL
QGRKRSIKSALLDQALVAGVGNIYADEALFMSEIRPTTACHQLQTEQVQRLRTAIIEVLSTSIGAGGTTFSDFRDLKGVN
GNYGGMAWVYGRQGQPCRTCGQTIERIKLVGRSTHFCPQCQPES
>Mature_283_residues
PELPEVETVRLGLEKVTVGMQIMGGEVLYPRTIAHPQSPQVFIQGLQDATFLSWMRRGKYLLSQLSFSTQQPSGWLGVHL
RMTGQLLWVAQDEPVQKHTRVRLFFVNNRELRFVDQRTFGQMWWVAPTEDPKQVISGLQKLGPEPFSEEFSVDYFWESLQ
GRKRSIKSALLDQALVAGVGNIYADEALFMSEIRPTTACHQLQTEQVQRLRTAIIEVLSTSIGAGGTTFSDFRDLKGVNG
NYGGMAWVYGRQGQPCRTCGQTIERIKLVGRSTHFCPQCQPES

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=281, Percent_Identity=40.2135231316726, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI1786932, Length=157, Percent_Identity=28.6624203821656, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_ACAM1 (B0C5D4)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516824.1
- ProteinModelPortal:   B0C5D4
- SMR:   B0C5D4
- GeneID:   5681314
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2501
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- ProtClustDB:   PRK13945
- BioCyc:   AMAR329726:AM1_2501-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 32098; Mature: 31967

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 60-60 ACT_SITE 272-272 BINDING 99-99 BINDING 118-118 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETVRLGLEKVTVGMQIMGGEVLYPRTIAHPQSPQVFIQGLQDATFLSWMRRGK
CCCCCCHHHHHHHHHHHHEEEEEECCEEECCHHCCCCCCCHHHHHCCCHHHHHHHHHHHH
YLLSQLSFSTQQPSGWLGVHLRMTGQLLWVAQDEPVQKHTRVRLFFVNNRELRFVDQRTF
HHHHHHHCCCCCCCCEEEEEEEEECEEEEEECCCCCCCCCEEEEEEECCCEEEEEECCCC
GQMWWVAPTEDPKQVISGLQKLGPEPFSEEFSVDYFWESLQGRKRSIKSALLDQALVAGV
CCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GNIYADEALFMSEIRPTTACHQLQTEQVQRLRTAIIEVLSTSIGAGGTTFSDFRDLKGVN
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
GNYGGMAWVYGRQGQPCRTCGQTIERIKLVGRSTHFCPQCQPES
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PELPEVETVRLGLEKVTVGMQIMGGEVLYPRTIAHPQSPQVFIQGLQDATFLSWMRRGK
CCCCCHHHHHHHHHHHHEEEEEECCEEECCHHCCCCCCCHHHHHCCCHHHHHHHHHHHH
YLLSQLSFSTQQPSGWLGVHLRMTGQLLWVAQDEPVQKHTRVRLFFVNNRELRFVDQRTF
HHHHHHHCCCCCCCCEEEEEEEEECEEEEEECCCCCCCCCEEEEEEECCCEEEEEECCCC
GQMWWVAPTEDPKQVISGLQKLGPEPFSEEFSVDYFWESLQGRKRSIKSALLDQALVAGV
CCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GNIYADEALFMSEIRPTTACHQLQTEQVQRLRTAIIEVLSTSIGAGGTTFSDFRDLKGVN
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
GNYGGMAWVYGRQGQPCRTCGQTIERIKLVGRSTHFCPQCQPES
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA