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The map label for this gene is miaE

Identifier: 158335633

GI number: 158335633

Start: 2509744

End: 2510319

Strand: Direct

Name: miaE

Synonym: AM1_2483

Alternate gene names: NA

Gene position: 2509744-2510319 (Clockwise)

Preceding gene: 158335632

Following gene: 158335634

Centisome position: 38.59

GC content: 48.44

Gene sequence:

>576_bases
GTGACCATTCACCTACATTGCGATAGTTCAGACGCTTGGCTGCAAACGGTTCTGGCAGACTTTGACACTTTCCTGCTCGA
TCATGCCGCCAACGAGAAAAAAGCCTCTGGGGTCGCCCTCAACCTTGCAGCCCACTATCCTGACAAGCCAGACCTAGTGG
CGGCCATGATTGACTTAGCCATTGAGGAGTTGAGCCATTATCGCGATGTCTTCAAATTAATCCGAGAACGCCACTTAACT
ATTCCTCCCGATGCCAAAGACCCCTACGTCAATCAACTGCGAGGTCTGATTCGTAAAGGCTCCGAAAATTACTTTCTCGA
TCGGCTCCTGGTCGCCGGAATTATTGAGGCTCGGGGCCTAGAACGCTTCACTAAAATTGCTTTAGCTTTGCCGAGTGGAC
CGTTAAAAGGCTTTTACCAAGCCATTGCCCATTCAGAGGCCAGGCATGCAAACTTATTTTTCAAACTCGCAGAACAATAT
TGTCCAAATGTAGCCCTACAGGCTCGTCTAGATGAACTCTTAGCGACTGAAATGAAGATTTTGAATCAATTACCCCATCG
GGCCATGCTGCATTAG

Upstream 100 bases:

>100_bases
CATGGCCCGGTCCAATCTTCAAACCGCTATCCGGTGTCTACAAGCTGGCATCCTGACCAACAATGGGGATAATAAATAAA
TTCAAACAGAGGACGTCTGT

Downstream 100 bases:

>100_bases
AGGATAGGAGGCAGCATTCTGCTTGTTATGGCGAAAGCAGACACGCTATTGTGCGGTGGTCTCCGATCCTTCTCTATGCT
TCTGCCCCCTCATGATCTCT

Product: tRNA-(ms(2)io(6)A)-hydroxylase

Products: NA

Alternate protein names: TRNA-(MS[2]IO[6]A)-Hydroxylase; TRNA-(Ms[2]Io[6]A)-Hydroxylase; TRNA-(Ms(2)Io(6)A)-Hydroxylase; TRNA-(MS[2]IO[6]A)-Hydroxylase Subfamily; TRNA-(MS(2)IO(6)A)-Hydroxylase-Like; TRNA-(MS[2]IO[6]A)-Hydroxylase-Like Protein; tRNA Hydroxylase; Probably TRNA-(MS[2]IO[6]A)-Hydroxylase; TRNA-(MS(2)IO(6)A)-Hydroxylase; Hydroxylase; tRNA Hydroxylase-Like Protein

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MTIHLHCDSSDAWLQTVLADFDTFLLDHAANEKKASGVALNLAAHYPDKPDLVAAMIDLAIEELSHYRDVFKLIRERHLT
IPPDAKDPYVNQLRGLIRKGSENYFLDRLLVAGIIEARGLERFTKIALALPSGPLKGFYQAIAHSEARHANLFFKLAEQY
CPNVALQARLDELLATEMKILNQLPHRAMLH

Sequences:

>Translated_191_residues
MTIHLHCDSSDAWLQTVLADFDTFLLDHAANEKKASGVALNLAAHYPDKPDLVAAMIDLAIEELSHYRDVFKLIRERHLT
IPPDAKDPYVNQLRGLIRKGSENYFLDRLLVAGIIEARGLERFTKIALALPSGPLKGFYQAIAHSEARHANLFFKLAEQY
CPNVALQARLDELLATEMKILNQLPHRAMLH
>Mature_190_residues
TIHLHCDSSDAWLQTVLADFDTFLLDHAANEKKASGVALNLAAHYPDKPDLVAAMIDLAIEELSHYRDVFKLIRERHLTI
PPDAKDPYVNQLRGLIRKGSENYFLDRLLVAGIIEARGLERFTKIALALPSGPLKGFYQAIAHSEARHANLFFKLAEQYC
PNVALQARLDELLATEMKILNQLPHRAMLH

Specific function: Unknown

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21547; Mature: 21416

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIHLHCDSSDAWLQTVLADFDTFLLDHAANEKKASGVALNLAAHYPDKPDLVAAMIDLA
CEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCCCHHHHHHHHHHH
IEELSHYRDVFKLIRERHLTIPPDAKDPYVNQLRGLIRKGSENYFLDRLLVAGIIEARGL
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ERFTKIALALPSGPLKGFYQAIAHSEARHANLFFKLAEQYCPNVALQARLDELLATEMKI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LNQLPHRAMLH
HHHCCHHHCCC
>Mature Secondary Structure 
TIHLHCDSSDAWLQTVLADFDTFLLDHAANEKKASGVALNLAAHYPDKPDLVAAMIDLA
EEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCCCHHHHHHHHHHH
IEELSHYRDVFKLIRERHLTIPPDAKDPYVNQLRGLIRKGSENYFLDRLLVAGIIEARGL
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ERFTKIALALPSGPLKGFYQAIAHSEARHANLFFKLAEQYCPNVALQARLDELLATEMKI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LNQLPHRAMLH
HHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA