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The map label for this gene is flhA [H]

Identifier: 158335613

GI number: 158335613

Start: 2480852

End: 2481961

Strand: Direct

Name: flhA [H]

Synonym: AM1_2463

Alternate gene names: 158335613

Gene position: 2480852-2481961 (Clockwise)

Preceding gene: 158335612

Following gene: 158335614

Centisome position: 38.15

GC content: 50.81

Gene sequence:

>1110_bases
ATGAAGGTCAAAGCAGCGGTGGCCCAAGCAGCCGGACAGCCCCTCAGTATTGAGACAGTCGATTTGGAAGGCCCCCAAGC
AGGGGAAGTCTTGGTGGAGATTAAGGCGACGGGGGTTTGCCATACCGATGCTTACACCTTGTCGGGTGCGGATCCAGAAG
GATTGTTCCCTGCCATCTTGGGGCATGAAGGCGCAGGTGTGGTCGTTGAGGTGGGGCCTGAGGTTAAGTCCCTTAAGCCT
GGCGATCATGTGATTCCGCTCTATGTACCCGAGTGCCGAACCTGTGAATATTGCTTGAGTGGTAAAACCAACCTTTGTCA
GTCCATTCGTGTGACCCAAGGTAAAGGGTTAATGCCCAATGGCTCTAGCCGGTTTTCTCTGGGCAGTCAGTCACTATTTC
ACTATATGGGTACCTCCACCTTTGCCAACTACACCGTGGTGCCTGAAATTTCCCTAGCCAAAATTCGCAAAGATGCCCCT
TTTGATAAGGTCTGTTTGATTGGCTGTGGAGTAACCACAGGTATTGGTGCTGTGATCAATACAGCCAAAGTAGAACCGGG
TGCCAATGTGGTCGTCTTTGGGTTAGGGGGTATTGGTCTCAACGTGATTCAGGCTGCCCGGCTGGTGGGTGCCAATATGA
TTATTGGGGTCGATCTGAATCCCGCCAAGCGGCCTCTGGCAGAGAAATTGGGCATGACCCATTTCGTTAATCCCAAGGAG
GTAGAAGGAGATTTAGTTCCCTATCTCGTAGATTTAACGAAAGGCGGGGCTGACTATAGCTTTGAGTGCATCGGCAATGT
CGAGGTGATGCGCCAAGCTTTGGAATGCTGCCACAAAGGCTGGGGAGAAAGCATCATTATTGGGGTTGCAGGTGCGGGTC
AAGAAATTAGTACCCGCCCTTTTCAGTTAGTCACCGGACGTGTTTGGAAAGGCACAGCCTTTGGAGGGGCTAAGGGACGC
ACGGATGTGCCTAAGATTGTCGATTGGTATATGGATGGCAAGATCGATATCGATTCTTTAGTCACCCACACCATGCCCCT
AGAAAAAATCAACGATGCCTTTGACTTAATGCATAAGGGCGAATCGATTCGGAGCGTTATCAATTTCTAG

Upstream 100 bases:

>100_bases
TCCTCCTACATTTAGCGGTTGGTAAAACTTGTAAACCTGTTGATCGACTGCATCTGAGAGCGCGTATAGTGAAAAACGCG
TACCCCTTAGGAGATCTGAG

Downstream 100 bases:

>100_bases
GGTGCTGTTGAGCATTGGACTCTTCTGTTTATAAAGCATGGCTTGATTAAAGCTGCTGATACATCGCTTTTAGCGGAGGT
AATAGGGGATAAGGCTGCAC

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKVKAAVAQAAGQPLSIETVDLEGPQAGEVLVEIKATGVCHTDAYTLSGADPEGLFPAILGHEGAGVVVEVGPEVKSLKP
GDHVIPLYVPECRTCEYCLSGKTNLCQSIRVTQGKGLMPNGSSRFSLGSQSLFHYMGTSTFANYTVVPEISLAKIRKDAP
FDKVCLIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVIQAARLVGANMIIGVDLNPAKRPLAEKLGMTHFVNPKE
VEGDLVPYLVDLTKGGADYSFECIGNVEVMRQALECCHKGWGESIIIGVAGAGQEISTRPFQLVTGRVWKGTAFGGAKGR
TDVPKIVDWYMDGKIDIDSLVTHTMPLEKINDAFDLMHKGESIRSVINF

Sequences:

>Translated_369_residues
MKVKAAVAQAAGQPLSIETVDLEGPQAGEVLVEIKATGVCHTDAYTLSGADPEGLFPAILGHEGAGVVVEVGPEVKSLKP
GDHVIPLYVPECRTCEYCLSGKTNLCQSIRVTQGKGLMPNGSSRFSLGSQSLFHYMGTSTFANYTVVPEISLAKIRKDAP
FDKVCLIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVIQAARLVGANMIIGVDLNPAKRPLAEKLGMTHFVNPKE
VEGDLVPYLVDLTKGGADYSFECIGNVEVMRQALECCHKGWGESIIIGVAGAGQEISTRPFQLVTGRVWKGTAFGGAKGR
TDVPKIVDWYMDGKIDIDSLVTHTMPLEKINDAFDLMHKGESIRSVINF
>Mature_369_residues
MKVKAAVAQAAGQPLSIETVDLEGPQAGEVLVEIKATGVCHTDAYTLSGADPEGLFPAILGHEGAGVVVEVGPEVKSLKP
GDHVIPLYVPECRTCEYCLSGKTNLCQSIRVTQGKGLMPNGSSRFSLGSQSLFHYMGTSTFANYTVVPEISLAKIRKDAP
FDKVCLIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVIQAARLVGANMIIGVDLNPAKRPLAEKLGMTHFVNPKE
VEGDLVPYLVDLTKGGADYSFECIGNVEVMRQALECCHKGWGESIIIGVAGAGQEISTRPFQLVTGRVWKGTAFGGAKGR
TDVPKIVDWYMDGKIDIDSLVTHTMPLEKINDAFDLMHKGESIRSVINF

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=369, Percent_Identity=60.4336043360434, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=46.7914438502674, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=44.7154471544715, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=44.7154471544715, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=44.4444444444444, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=44.4444444444444, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=43.6314363143631, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI156523966, Length=367, Percent_Identity=43.5967302452316, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=43.7673130193906, Blast_Score=320, Evalue=1e-87,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=63.4146341463415, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI87082125, Length=380, Percent_Identity=26.5789473684211, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788073, Length=380, Percent_Identity=29.2105263157895, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790045, Length=366, Percent_Identity=27.0491803278689, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI226510992, Length=350, Percent_Identity=25.4285714285714, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787863, Length=352, Percent_Identity=24.1477272727273, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786825, Length=377, Percent_Identity=26.2599469496021, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081918, Length=220, Percent_Identity=33.6363636363636, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788075, Length=385, Percent_Identity=24.9350649350649, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788407, Length=231, Percent_Identity=28.1385281385281, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786518, Length=376, Percent_Identity=27.1276595744681, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790718, Length=362, Percent_Identity=23.2044198895028, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=58.7131367292225, Blast_Score=452, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=58.4, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=58.0174927113703, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17562584, Length=370, Percent_Identity=28.6486486486486, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71988145, Length=371, Percent_Identity=27.2237196765499, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562876, Length=363, Percent_Identity=26.4462809917355, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=372, Percent_Identity=27.1505376344086, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17562878, Length=365, Percent_Identity=25.4794520547945, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=372, Percent_Identity=53.763440860215, Blast_Score=434, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6319621, Length=367, Percent_Identity=30.7901907356948, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323729, Length=363, Percent_Identity=27.8236914600551, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=26.9230769230769, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6322619, Length=355, Percent_Identity=28.7323943661972, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=363, Percent_Identity=26.7217630853994, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6319955, Length=355, Percent_Identity=28.7323943661972, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323099, Length=360, Percent_Identity=28.3333333333333, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319257, Length=373, Percent_Identity=25.201072386059, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319258, Length=265, Percent_Identity=27.5471698113208, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=61.9565217391304, Blast_Score=481, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39085; Mature: 39085

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKAAVAQAAGQPLSIETVDLEGPQAGEVLVEIKATGVCHTDAYTLSGADPEGLFPAIL
CCCHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCEEECCCEEECCCCCCCCCHHHH
GHEGAGVVVEVGPEVKSLKPGDHVIPLYVPECRTCEYCLSGKTNLCQSIRVTQGKGLMPN
CCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHCCCHHHHHHEEEECCCCCCCC
GSSRFSLGSQSLFHYMGTSTFANYTVVPEISLAKIRKDAPFDKVCLIGCGVTTGIGAVIN
CCCCCCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCEEEEECCHHHHHHHHEE
TAKVEPGANVVVFGLGGIGLNVIQAARLVGANMIIGVDLNPAKRPLAEKLGMTHFVNPKE
EEEECCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCEECCCHHH
VEGDLVPYLVDLTKGGADYSFECIGNVEVMRQALECCHKGWGESIIIGVAGAGQEISTRP
CCCCCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
FQLVTGRVWKGTAFGGAKGRTDVPKIVDWYMDGKIDIDSLVTHTMPLEKINDAFDLMHKG
EEEEECCEEECCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCC
ESIRSVINF
HHHHHHHCC
>Mature Secondary Structure
MKVKAAVAQAAGQPLSIETVDLEGPQAGEVLVEIKATGVCHTDAYTLSGADPEGLFPAIL
CCCHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCEEECCCEEECCCCCCCCCHHHH
GHEGAGVVVEVGPEVKSLKPGDHVIPLYVPECRTCEYCLSGKTNLCQSIRVTQGKGLMPN
CCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHCCCHHHHHHEEEECCCCCCCC
GSSRFSLGSQSLFHYMGTSTFANYTVVPEISLAKIRKDAPFDKVCLIGCGVTTGIGAVIN
CCCCCCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCEEEEECCHHHHHHHHEE
TAKVEPGANVVVFGLGGIGLNVIQAARLVGANMIIGVDLNPAKRPLAEKLGMTHFVNPKE
EEEECCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCEECCCHHH
VEGDLVPYLVDLTKGGADYSFECIGNVEVMRQALECCHKGWGESIIIGVAGAGQEISTRP
CCCCCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
FQLVTGRVWKGTAFGGAKGRTDVPKIVDWYMDGKIDIDSLVTHTMPLEKINDAFDLMHKG
EEEEECCEEECCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCC
ESIRSVINF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]