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The map label for this gene is thiD [H]

Identifier: 158335594

GI number: 158335594

Start: 2454659

End: 2455492

Strand: Direct

Name: thiD [H]

Synonym: AM1_2444

Alternate gene names: 158335594

Gene position: 2454659-2455492 (Clockwise)

Preceding gene: 158335593

Following gene: 158335595

Centisome position: 37.74

GC content: 52.52

Gene sequence:

>834_bases
ATGATACCCATCGCACTAACCATTGCTGGCTCCGACAGCGGTGGTGGAGCGGGTATCCAAGCTGACCTGCGGACCTTTGC
TTTTCACCGGGTTCACGGCACCAGTGCCCTCACCTGTGTGACAGCCCAAAATACCGTGGGAGTGAATCGGGTGGATGCCT
TACCCGCAGCAGCAGTAGTGGCTCAAATCGATGCAGTTGCCTCTGATATTGGTGTGCAAGCAGCCAAAACAGGGATGCTG
CTCAATGCAGAGATTATGGAAGCAGTGGCGGCGGCAGTTCAGCAACAGGATATTCACCAGCTAGTTGTTGATCCGGTGAT
GGTGTCGCGGGCTGGAGTAGTGCTGATTGATGAGGTTGCGATCGCAACCCTCAAAACCCACCTACTCCCCCAAGCCTTAA
TCCTCACCCCTAATCGCTATGAAGCCCAAATCCTGTGTGGTATAAAGATTCAGACTCTCGAAGATATGCAGAATGCTGCC
CAAAACATCTTCGATATGGGACCTAAAAATGTCTTAGTCAAGGGCGGAGGGATGAGCGATACGCTTTTAGGTATCGATGT
TTGGTTTGATGGCCAACAGATGGAGACCCTGAAGACGGAAACCGTTGCTACCACGAATACCCACGGCACGGGTTGTACGT
TGTCGGCTGCGATCGCAGCCAATCTCGCCCTAGGCAAATCCCCCCTAGCAGCTGCTAAAGCCGCAAAAACCTACGTCACT
CAAGCCTTGAAATATGCCTTAGATATTGGTCAAGGTCAAGGCCCTGTCGGCCATTGGTTTCCCCTCGTCTCTAAAGCACC
AGAAGCCTTAGGCTTTTCCCGCCTCTGGAGTTAA

Upstream 100 bases:

>100_bases
ATTGCTAGCTGCAAACCCTCGTGTTTCGGCCACGGCAATTCAAACTATAGGAAGTAAAGGCTATTAGGCGTTCATCTGAT
AACAAATGGTGGTAAATCAA

Downstream 100 bases:

>100_bases
TCCTCAAAAGTTACAGTGTGCAAGGAAATAGCTATAAGTAGTCAGGTTCCATATCAAGCTGCACAGACAAAGCTCTCTCT
TTCTTGAGATAATTATCCAA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MIPIALTIAGSDSGGGAGIQADLRTFAFHRVHGTSALTCVTAQNTVGVNRVDALPAAAVVAQIDAVASDIGVQAAKTGML
LNAEIMEAVAAAVQQQDIHQLVVDPVMVSRAGVVLIDEVAIATLKTHLLPQALILTPNRYEAQILCGIKIQTLEDMQNAA
QNIFDMGPKNVLVKGGGMSDTLLGIDVWFDGQQMETLKTETVATTNTHGTGCTLSAAIAANLALGKSPLAAAKAAKTYVT
QALKYALDIGQGQGPVGHWFPLVSKAPEALGFSRLWS

Sequences:

>Translated_277_residues
MIPIALTIAGSDSGGGAGIQADLRTFAFHRVHGTSALTCVTAQNTVGVNRVDALPAAAVVAQIDAVASDIGVQAAKTGML
LNAEIMEAVAAAVQQQDIHQLVVDPVMVSRAGVVLIDEVAIATLKTHLLPQALILTPNRYEAQILCGIKIQTLEDMQNAA
QNIFDMGPKNVLVKGGGMSDTLLGIDVWFDGQQMETLKTETVATTNTHGTGCTLSAAIAANLALGKSPLAAAKAAKTYVT
QALKYALDIGQGQGPVGHWFPLVSKAPEALGFSRLWS
>Mature_277_residues
MIPIALTIAGSDSGGGAGIQADLRTFAFHRVHGTSALTCVTAQNTVGVNRVDALPAAAVVAQIDAVASDIGVQAAKTGML
LNAEIMEAVAAAVQQQDIHQLVVDPVMVSRAGVVLIDEVAIATLKTHLLPQALILTPNRYEAQILCGIKIQTLEDMQNAA
QNIFDMGPKNVLVKGGGMSDTLLGIDVWFDGQQMETLKTETVATTNTHGTGCTLSAAIAANLALGKSPLAAAKAAKTYVT
QALKYALDIGQGQGPVGHWFPLVSKAPEALGFSRLWS

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:
GO:0008972: Phosphomethylpyrimidine kinase
GO:0009228: Phosphomethylpyrimidine kinase
GO:0016301: Phosphomethylpyrimidine kinase
GO:0016310: Phosphomethylpyrimidine kinase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=258, Percent_Identity=40.6976744186046, Blast_Score=171, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325378, Length=275, Percent_Identity=32.3636363636364, Blast_Score=122, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6324517, Length=274, Percent_Identity=34.3065693430657, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=34.6715328467153, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28776; Mature: 28776

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIALTIAGSDSGGGAGIQADLRTFAFHRVHGTSALTCVTAQNTVGVNRVDALPAAAVV
CCEEEEEEECCCCCCCCCHHHHHHHHHHHEECCCCEEEEEEECCCCCCCHHHHHHHHHHH
AQIDAVASDIGVQAAKTGMLLNAEIMEAVAAAVQQQDIHQLVVDPVMVSRAGVVLIDEVA
HHHHHHHHHHCHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
IATLKTHLLPQALILTPNRYEAQILCGIKIQTLEDMQNAAQNIFDMGPKNVLVKGGGMSD
HHHHHHHHCCCEEEECCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
TLLGIDVWFDGQQMETLKTETVATTNTHGTGCTLSAAIAANLALGKSPLAAAKAAKTYVT
EEEEEEEEECCCHHHHHHHHEEEECCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHH
QALKYALDIGQGQGPVGHWFPLVSKAPEALGFSRLWS
HHHHHHHHCCCCCCCCCHHHHHHHCCHHHHCHHHHCC
>Mature Secondary Structure
MIPIALTIAGSDSGGGAGIQADLRTFAFHRVHGTSALTCVTAQNTVGVNRVDALPAAAVV
CCEEEEEEECCCCCCCCCHHHHHHHHHHHEECCCCEEEEEEECCCCCCCHHHHHHHHHHH
AQIDAVASDIGVQAAKTGMLLNAEIMEAVAAAVQQQDIHQLVVDPVMVSRAGVVLIDEVA
HHHHHHHHHHCHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
IATLKTHLLPQALILTPNRYEAQILCGIKIQTLEDMQNAAQNIFDMGPKNVLVKGGGMSD
HHHHHHHHCCCEEEECCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
TLLGIDVWFDGQQMETLKTETVATTNTHGTGCTLSAAIAANLALGKSPLAAAKAAKTYVT
EEEEEEEEECCCHHHHHHHHEEEECCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHH
QALKYALDIGQGQGPVGHWFPLVSKAPEALGFSRLWS
HHHHHHHHCCCCCCCCCHHHHHHHCCHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA