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The map label for this gene is phrB [H]

Identifier: 158335578

GI number: 158335578

Start: 2432324

End: 2433778

Strand: Reverse

Name: phrB [H]

Synonym: AM1_2428

Alternate gene names: 158335578

Gene position: 2433778-2432324 (Counterclockwise)

Preceding gene: 158335579

Following gene: 158335575

Centisome position: 37.42

GC content: 51.82

Gene sequence:

>1455_bases
GTGCGAGTCCTCATTTGGTTTCGCAATGACTTGCGGCTCCATGATCATGCGCCGCTCCATCAAGCGGTGCGTTCCAATGC
CGATGTGATTCCTTGCTACTGCTTTGACCCCCGTCAGTTCGGCCAAACCCCCTTTGGCTTTCCCAAAACGGGACCGTTTA
GAGCCCAGTTTTTATTGGAGAGTGTGGCAGATTTGCGTCAATCCTTGCGAGGCAAGCAGAGTGATCTGATCCTGCGCCAG
GGTCATCCCGAAACGGTTCTGCCTGAGTTGGCCCAAGCCTTGAAGGTGGAGACGGTGTATTTCAACCGGGAAGTTACGGC
TGAGGAAATTGAGGTCGAGAATCGTTTGCGTTCTGCTTTAGCCGATCTAGGGATTGAGTGCCTACGTTTTTGGAGCAGTA
CGCTCTTTCACCCAGAGCAGCTGCCTTTTCCCATCCGAGAGCTGCCAGAAGTATTCACCCAGTTTCGCAAGCAGGTGGAG
AAATCTGCCAAGCCCAAAGCCCCCTTCCCCACCCCCCAGTCTTTATCGACGCTACCCGATATTGCTCCAGGTGAGCTGCC
TCAACTGGAGGACTGGGGATTATCGTCTCCTGAGCCTGACGCCAGAGCGATGATTCAATTCTCCGGGGGAGAAACGGCAG
CACTGGCCCGATTGCAGGACTATATCTGGGAGCAGGATCGCCTCAAGCGCTATAAGGAAACTCGTAACGGCATGCTGCAG
CCGAATGATTCCACGAAGTTTTCGCCGTGGTTGGCTTTGGGCTGTGTATCGCCGCGATATATTCACCAGCAGGTCAAGAC
CTATGAGCAAGACCGGGTTAAAAATAACTCCACGTACTGGCTGATTTTTGAGCTGATTTGGCGCGATTATTTCCGGTTTA
TTTGTGCCAAGCATGGCAAGCGAGTCTTTCGGGTATCGGGGTTGCAGGGGCTGGCATTGCCCTGGCAGGACAACCGCGAA
ACCTTTGAGAAATGGCAAACGGGCCAGACGGGATTTCCGCTAATTGATGCCAATATGCGGGAGTTGGCGGCGACGGGGTT
TATGTCGAATCGGGGACGGCAGAATGTGGCCAGCTTTTTAACCAAGAATTTGGGTCTGAATTGGCAAATGGGGGCAGAAT
GGTTTGAATCTTGCCTGATTGATTACGATGTGTGCAGCAATTGGGGCAATTGGAATTATGCGGCGGGAGTGGGAAATGAT
GCCCGAGGATTTCGGTTTTTCAATATTGTTAAACAGGCAAAGGATTATGACCCGCAAGGGGATTATGTGCGCCATTGGTT
ACCGGAGTTGAAGGATGTACCAGGGGGACAGGTGCAGACGCCTTGGCAGTTAACAGCATTGGAGCAAGAGATGTTTGGCT
TGACGATTGGCAAGGATTATCCAGAACCAATGGTAGATTTGTGGCAGTCAGCTAAGGTAAATGAGCAGATCTATAACCGA
GTGGTGAATTATTAG

Upstream 100 bases:

>100_bases
TGTCGGCCATTCTCAACTATAACCGAGCTTTAATCGCCCTCCAGCGGGCCACGAGCTTTGCTCAACCCGTTAGTGGTGTA
ACGCCTCTAGGGGGATAAGG

Downstream 100 bases:

>100_bases
TGAATTATTAAAACCAAGTGGTGAATTATTGGTGAATTATTAATAGTTGACTCTAGAAGTGATGTAGAGAATTTCTTGCA
CATTTTTGAGATCTTGAAAA

Product: deoxyribodipyrimidine photolyase

Products: 2 pyrimidine residues (in DNA) [C]

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MRVLIWFRNDLRLHDHAPLHQAVRSNADVIPCYCFDPRQFGQTPFGFPKTGPFRAQFLLESVADLRQSLRGKQSDLILRQ
GHPETVLPELAQALKVETVYFNREVTAEEIEVENRLRSALADLGIECLRFWSSTLFHPEQLPFPIRELPEVFTQFRKQVE
KSAKPKAPFPTPQSLSTLPDIAPGELPQLEDWGLSSPEPDARAMIQFSGGETAALARLQDYIWEQDRLKRYKETRNGMLQ
PNDSTKFSPWLALGCVSPRYIHQQVKTYEQDRVKNNSTYWLIFELIWRDYFRFICAKHGKRVFRVSGLQGLALPWQDNRE
TFEKWQTGQTGFPLIDANMRELAATGFMSNRGRQNVASFLTKNLGLNWQMGAEWFESCLIDYDVCSNWGNWNYAAGVGND
ARGFRFFNIVKQAKDYDPQGDYVRHWLPELKDVPGGQVQTPWQLTALEQEMFGLTIGKDYPEPMVDLWQSAKVNEQIYNR
VVNY

Sequences:

>Translated_484_residues
MRVLIWFRNDLRLHDHAPLHQAVRSNADVIPCYCFDPRQFGQTPFGFPKTGPFRAQFLLESVADLRQSLRGKQSDLILRQ
GHPETVLPELAQALKVETVYFNREVTAEEIEVENRLRSALADLGIECLRFWSSTLFHPEQLPFPIRELPEVFTQFRKQVE
KSAKPKAPFPTPQSLSTLPDIAPGELPQLEDWGLSSPEPDARAMIQFSGGETAALARLQDYIWEQDRLKRYKETRNGMLQ
PNDSTKFSPWLALGCVSPRYIHQQVKTYEQDRVKNNSTYWLIFELIWRDYFRFICAKHGKRVFRVSGLQGLALPWQDNRE
TFEKWQTGQTGFPLIDANMRELAATGFMSNRGRQNVASFLTKNLGLNWQMGAEWFESCLIDYDVCSNWGNWNYAAGVGND
ARGFRFFNIVKQAKDYDPQGDYVRHWLPELKDVPGGQVQTPWQLTALEQEMFGLTIGKDYPEPMVDLWQSAKVNEQIYNR
VVNY
>Mature_484_residues
MRVLIWFRNDLRLHDHAPLHQAVRSNADVIPCYCFDPRQFGQTPFGFPKTGPFRAQFLLESVADLRQSLRGKQSDLILRQ
GHPETVLPELAQALKVETVYFNREVTAEEIEVENRLRSALADLGIECLRFWSSTLFHPEQLPFPIRELPEVFTQFRKQVE
KSAKPKAPFPTPQSLSTLPDIAPGELPQLEDWGLSSPEPDARAMIQFSGGETAALARLQDYIWEQDRLKRYKETRNGMLQ
PNDSTKFSPWLALGCVSPRYIHQQVKTYEQDRVKNNSTYWLIFELIWRDYFRFICAKHGKRVFRVSGLQGLALPWQDNRE
TFEKWQTGQTGFPLIDANMRELAATGFMSNRGRQNVASFLTKNLGLNWQMGAEWFESCLIDYDVCSNWGNWNYAAGVGND
ARGFRFFNIVKQAKDYDPQGDYVRHWLPELKDVPGGQVQTPWQLTALEQEMFGLTIGKDYPEPMVDLWQSAKVNEQIYNR
VVNY

Specific function: May have a photoreceptor function. Binds DNA; probably functions as a transcriptional repressor [H]

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=499, Percent_Identity=30.060120240481, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI4758072, Length=504, Percent_Identity=29.1666666666667, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI188536103, Length=448, Percent_Identity=29.4642857142857, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1786926, Length=475, Percent_Identity=30.7368421052632, Blast_Score=195, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6324962, Length=514, Percent_Identity=27.6264591439689, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17137248, Length=478, Percent_Identity=28.8702928870293, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24585455, Length=478, Percent_Identity=28.8702928870293, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24648152, Length=511, Percent_Identity=28.7671232876712, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR014133
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: 4.1.99.3 [C]

Molecular weight: Translated: 56041; Mature: 56041

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIWFRNDLRLHDHAPLHQAVRSNADVIPCYCFDPRQFGQTPFGFPKTGPFRAQFLLE
CEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEEECHHHHCCCCCCCCCCCCHHHHHHHH
SVADLRQSLRGKQSDLILRQGHPETVLPELAQALKVETVYFNREVTAEEIEVENRLRSAL
HHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHH
ADLGIECLRFWSSTLFHPEQLPFPIRELPEVFTQFRKQVEKSAKPKAPFPTPQSLSTLPD
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
IAPGELPQLEDWGLSSPEPDARAMIQFSGGETAALARLQDYIWEQDRLKRYKETRNGMLQ
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
PNDSTKFSPWLALGCVSPRYIHQQVKTYEQDRVKNNSTYWLIFELIWRDYFRFICAKHGK
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCC
RVFRVSGLQGLALPWQDNRETFEKWQTGQTGFPLIDANMRELAATGFMSNRGRQNVASFL
EEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHCCCHHHHHHHH
TKNLGLNWQMGAEWFESCLIDYDVCSNWGNWNYAAGVGNDARGFRFFNIVKQAKDYDPQG
HHHCCCCCCHHHHHHHHHHHCHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCC
DYVRHWLPELKDVPGGQVQTPWQLTALEQEMFGLTIGKDYPEPMVDLWQSAKVNEQIYNR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHH
VVNY
HCCC
>Mature Secondary Structure
MRVLIWFRNDLRLHDHAPLHQAVRSNADVIPCYCFDPRQFGQTPFGFPKTGPFRAQFLLE
CEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEEECHHHHCCCCCCCCCCCCHHHHHHHH
SVADLRQSLRGKQSDLILRQGHPETVLPELAQALKVETVYFNREVTAEEIEVENRLRSAL
HHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHH
ADLGIECLRFWSSTLFHPEQLPFPIRELPEVFTQFRKQVEKSAKPKAPFPTPQSLSTLPD
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
IAPGELPQLEDWGLSSPEPDARAMIQFSGGETAALARLQDYIWEQDRLKRYKETRNGMLQ
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
PNDSTKFSPWLALGCVSPRYIHQQVKTYEQDRVKNNSTYWLIFELIWRDYFRFICAKHGK
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCC
RVFRVSGLQGLALPWQDNRETFEKWQTGQTGFPLIDANMRELAATGFMSNRGRQNVASFL
EEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHCCCHHHHHHHH
TKNLGLNWQMGAEWFESCLIDYDVCSNWGNWNYAAGVGNDARGFRFFNIVKQAKDYDPQG
HHHCCCCCCHHHHHHHHHHHCHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCC
DYVRHWLPELKDVPGGQVQTPWQLTALEQEMFGLTIGKDYPEPMVDLWQSAKVNEQIYNR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHH
VVNY
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; FADH2; Flavin; pterin [C]

Metal ions: NA

Kcat value (1/min): 2.4 [C]

Specific activity: NA

Km value (mM): NA

Substrates: cyclobutadipyrimidine (in DNA) [C]

Specific reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) [C]

General reaction: C-C-bond cleavage [C]

Inhibitor: yeast DNA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]