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The map label for this gene is gidB

Identifier: 158335573

GI number: 158335573

Start: 2427902

End: 2428672

Strand: Direct

Name: gidB

Synonym: AM1_2423

Alternate gene names: 158335573

Gene position: 2427902-2428672 (Clockwise)

Preceding gene: 158335572

Following gene: 158335576

Centisome position: 37.33

GC content: 53.05

Gene sequence:

>771_bases
ATGAGCAACGGCGCCCTACCGAATCGATCTGACGTGTGGTTGCAATCTTTGGGCTGGCAGCCTACCCCTGAGCAGGAGCA
GCAGTTTGAACGGCTGTTTCAGCTGATTGTCCAAGCCAATCAGCAGCTGAATTTAACCCGGATTACAGATCCCCCAGACT
TCTGGGAAAAACATCTGTGGGATTCCCTCGTTGGAATTGCGCCTTGGCTACAGCCGGACTCTTCTATACAGCGGCAGGTT
AGCTTCTCGGTAGACCACAGCCACCCCTTAAAAGTCATTGATATTGGAACGGGAGGCGGGTTTCCGGGGCTACCCATCGC
CATTATTAAACCCACCTGGCGTATCACTCTGCTGGATTCGACCCAAAAGAAGCTGGCTTTTTTGCAGCAGGTCAACGCAG
AACTGGGCTTGGAGAATGTGGAGACGTGTTGCGATCGCGCCGAAAATCTAGGTCAACAGTCCCCCTATCGAGAATCCTTT
GATCTCGCCGTTATTCGGGCCGTAGGTCCGGCTTCGGTCTGTGCTGAATACGCCCTCCCTCTATTGGCATTGCAGGGCTA
TGCCGTACTGTATCGCGGTCAATGGACCGCAGAAGAGCAGGAAGGATTAGTGGCTGCAACCTCCAAACTAGGAGGCGAAC
TGATTGCCGTATCACCTGTTAAAATGCCTCATTCTCAAGGCATTCGGCACTGTATCTATCTTCGAAAGATTAAGACCACA
GACCCTAAATTTCCTCGGCGCGTAGGGGTGCCCGCCCGAAAGCCCCTGTAA

Upstream 100 bases:

>100_bases
TTACCCTCATCACCCATGATGCGGGGGGCTTGACGGAGATGGATTTCCGCATGGCCCAAACCATTTCTAATCTGTAGGTC
TGGCCCCGAACAGACCACCC

Downstream 100 bases:

>100_bases
CTTCGCACCTATTTAATGGCCAGTTAATACACCTAAAAATTCAGATTATCGATAGGCTTCTAAGCCACCGATCCAGGTGA
CGAGAGCCCGACGGGTGCCT

Product: methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSNGALPNRSDVWLQSLGWQPTPEQEQQFERLFQLIVQANQQLNLTRITDPPDFWEKHLWDSLVGIAPWLQPDSSIQRQV
SFSVDHSHPLKVIDIGTGGGFPGLPIAIIKPTWRITLLDSTQKKLAFLQQVNAELGLENVETCCDRAENLGQQSPYRESF
DLAVIRAVGPASVCAEYALPLLALQGYAVLYRGQWTAEEQEGLVAATSKLGGELIAVSPVKMPHSQGIRHCIYLRKIKTT
DPKFPRRVGVPARKPL

Sequences:

>Translated_256_residues
MSNGALPNRSDVWLQSLGWQPTPEQEQQFERLFQLIVQANQQLNLTRITDPPDFWEKHLWDSLVGIAPWLQPDSSIQRQV
SFSVDHSHPLKVIDIGTGGGFPGLPIAIIKPTWRITLLDSTQKKLAFLQQVNAELGLENVETCCDRAENLGQQSPYRESF
DLAVIRAVGPASVCAEYALPLLALQGYAVLYRGQWTAEEQEGLVAATSKLGGELIAVSPVKMPHSQGIRHCIYLRKIKTT
DPKFPRRVGVPARKPL
>Mature_255_residues
SNGALPNRSDVWLQSLGWQPTPEQEQQFERLFQLIVQANQQLNLTRITDPPDFWEKHLWDSLVGIAPWLQPDSSIQRQVS
FSVDHSHPLKVIDIGTGGGFPGLPIAIIKPTWRITLLDSTQKKLAFLQQVNAELGLENVETCCDRAENLGQQSPYRESFD
LAVIRAVGPASVCAEYALPLLALQGYAVLYRGQWTAEEQEGLVAATSKLGGELIAVSPVKMPHSQGIRHCIYLRKIKTTD
PKFPRRVGVPARKPL

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=231, Percent_Identity=29.004329004329, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_ACAM1 (B0C384)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516745.1
- ProteinModelPortal:   B0C384
- SMR:   B0C384
- GeneID:   5681235
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2423
- HOGENOM:   HBG686577
- OMA:   HLWDSLR
- BioCyc:   AMAR329726:AM1_2423-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 28539; Mature: 28408

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGALPNRSDVWLQSLGWQPTPEQEQQFERLFQLIVQANQQLNLTRITDPPDFWEKHLW
CCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHH
DSLVGIAPWLQPDSSIQRQVSFSVDHSHPLKVIDIGTGGGFPGLPIAIIKPTWRITLLDS
HHHHHHCCCCCCCHHHHHHEEEECCCCCCEEEEEECCCCCCCCCCEEEECCCEEEEEECC
TQKKLAFLQQVNAELGLENVETCCDRAENLGQQSPYRESFDLAVIRAVGPASVCAEYALP
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
LLALQGYAVLYRGQWTAEEQEGLVAATSKLGGELIAVSPVKMPHSQGIRHCIYLRKIKTT
HHHHCCEEEEEECCCCCHHHCCEEEEHHHCCCEEEEECCCCCCHHHHHHHHHHHHEECCC
DPKFPRRVGVPARKPL
CCCCCHHCCCCCCCCC
>Mature Secondary Structure 
SNGALPNRSDVWLQSLGWQPTPEQEQQFERLFQLIVQANQQLNLTRITDPPDFWEKHLW
CCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHH
DSLVGIAPWLQPDSSIQRQVSFSVDHSHPLKVIDIGTGGGFPGLPIAIIKPTWRITLLDS
HHHHHHCCCCCCCHHHHHHEEEECCCCCCEEEEEECCCCCCCCCCEEEECCCEEEEEECC
TQKKLAFLQQVNAELGLENVETCCDRAENLGQQSPYRESFDLAVIRAVGPASVCAEYALP
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
LLALQGYAVLYRGQWTAEEQEGLVAATSKLGGELIAVSPVKMPHSQGIRHCIYLRKIKTT
HHHHCCEEEEEECCCCCHHHCCEEEEHHHCCCEEEEECCCCCCHHHHHHHHHHHHEECCC
DPKFPRRVGVPARKPL
CCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA