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The map label for this gene is pilD [H]

Identifier: 158335569

GI number: 158335569

Start: 2424194

End: 2425033

Strand: Reverse

Name: pilD [H]

Synonym: AM1_2419

Alternate gene names: 158335569

Gene position: 2425033-2424194 (Counterclockwise)

Preceding gene: 158335570

Following gene: 158335568

Centisome position: 37.29

GC content: 52.62

Gene sequence:

>840_bases
ATGAGTTGGGCAGAAATCTTTATCTATGGACTGGTGGTGAGCTTGGGGGCTTCGGTGGGTAGTTTCTTGAACGTCGTCAT
TTATCGGCTACCCGAAGGCTTATCTCTACTTCATCCCCCTTCTCGTTGTCCCAAATGTCACACCCCCCTGAAACCCTATG
ACAATGTTCCGGTTCTAGGTTGGCTGTGGCTCCAAGGCAAATGTCGCTATTGTAAAACCCCGATAGCCAGCCGGTATCCC
CTCGTTGAAGCAGCCACGGCCTGCATGTTTCTGGGGATTTTCCTGCTGTTTGGCTATCAGCTGCAGACCTTGGGTTATTG
GCTGTTTGCCAGTGTGCTGATTTCCCTGGCCCTGATTGATTTAGATGTGATGTTATTGCCCAGTCCCTTGACCCGGTTGG
GGGTGATTTCGGGCGTCACGTTTCAGACCCTAGTGGGCTGGCATCAAAATGGCCTGACGGGGGCGGCCCAAGGGTTCACC
TTTGGTGCTTTGGGGGTGATTGCGGGGATCTGGTTGATTGATGTAATTGGCCTGTTGGCTGGCCTGATCTTTCGTCGTGC
TGCGATGGGGGGTGGCGATAGCAAACTGATGGCGATGATTGGTGCTTGGTTAGGGTGGCGATATATGTTGCTGACTCTGT
TTCTCGCCTGTTTGGTAGGGTCAGTGGTAGGGATAGGAGGCCGGGTATTTGGCAAACTTGGCAAGTTTCAGGTGATTCCT
TTTGGCCCGTTTCTGGTGCTGGGAGCATTGCTGTGCATGATGTCAGGGGCGCAAATTTGGACGGGTTATCAGCAGGGAAT
TTTGTGGCTGTATCGCTGGATGGGGATTGAATTGGGGTAA

Upstream 100 bases:

>100_bases
CTTCTCTGGTAGAGGTTGTCTCCCTTCAAACATCAAGGAGAAGCACTGAATAGATAGCCCTAATCTCCTCGGCGATCTGG
CTATCGGTGTGTACAAAGGT

Downstream 100 bases:

>100_bases
GGTCTAGCTGAATAGAGCACTAAGAAGGCTAGGCAGGATAATCCCTAAACTCCGATGGAGAACCGGAGTATACTAGGTCC
AAGGCTGTTGTTGCCTCAGG

Product: type IV pilus prepilin peptidase PilD

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSWAEIFIYGLVVSLGASVGSFLNVVIYRLPEGLSLLHPPSRCPKCHTPLKPYDNVPVLGWLWLQGKCRYCKTPIASRYP
LVEAATACMFLGIFLLFGYQLQTLGYWLFASVLISLALIDLDVMLLPSPLTRLGVISGVTFQTLVGWHQNGLTGAAQGFT
FGALGVIAGIWLIDVIGLLAGLIFRRAAMGGGDSKLMAMIGAWLGWRYMLLTLFLACLVGSVVGIGGRVFGKLGKFQVIP
FGPFLVLGALLCMMSGAQIWTGYQQGILWLYRWMGIELG

Sequences:

>Translated_279_residues
MSWAEIFIYGLVVSLGASVGSFLNVVIYRLPEGLSLLHPPSRCPKCHTPLKPYDNVPVLGWLWLQGKCRYCKTPIASRYP
LVEAATACMFLGIFLLFGYQLQTLGYWLFASVLISLALIDLDVMLLPSPLTRLGVISGVTFQTLVGWHQNGLTGAAQGFT
FGALGVIAGIWLIDVIGLLAGLIFRRAAMGGGDSKLMAMIGAWLGWRYMLLTLFLACLVGSVVGIGGRVFGKLGKFQVIP
FGPFLVLGALLCMMSGAQIWTGYQQGILWLYRWMGIELG
>Mature_278_residues
SWAEIFIYGLVVSLGASVGSFLNVVIYRLPEGLSLLHPPSRCPKCHTPLKPYDNVPVLGWLWLQGKCRYCKTPIASRYPL
VEAATACMFLGIFLLFGYQLQTLGYWLFASVLISLALIDLDVMLLPSPLTRLGVISGVTFQTLVGWHQNGLTGAAQGFTF
GALGVIAGIWLIDVIGLLAGLIFRRAAMGGGDSKLMAMIGAWLGWRYMLLTLFLACLVGSVVGIGGRVFGKLGKFQVIPF
GPFLVLGALLCMMSGAQIWTGYQQGILWLYRWMGIELG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:
GO:0004190: Type IV pilus prepilin peptidase PilD
GO:0008233: Type IV pilus prepilin peptidase PilD
GO:0016020: Type IV pilus prepilin peptidase PilD
GO:0016021: Type IV pilus prepilin peptidase PilD
GO:0016787: Type IV pilus prepilin peptidase PilD

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=258, Percent_Identity=34.8837209302326, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 30373; Mature: 30242

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SWAEIFIYGLVVSLGASVGSFLNVVIYRLPEGLSLLHPPSRCPKCHTPLKPYDNVPVLG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
WLWLQGKCRYCKTPIASRYPLVEAATACMFLGIFLLFGYQLQTLGYWLFASVLISLALID
EEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDVMLLPSPLTRLGVISGVTFQTLVGWHQNGLTGAAQGFTFGALGVIAGIWLIDVIGLLA
HHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHH
GLIFRRAAMGGGDSKLMAMIGAWLGWRYMLLTLFLACLVGSVVGIGGRVFGKLGKFQVIP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEC
FGPFLVLGALLCMMSGAQIWTGYQQGILWLYRWMGIELG
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]