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The map label for this gene is ppsA [H]

Identifier: 158335499

GI number: 158335499

Start: 2349702

End: 2352029

Strand: Direct

Name: ppsA [H]

Synonym: AM1_2347

Alternate gene names: 158335499

Gene position: 2349702-2352029 (Clockwise)

Preceding gene: 158335497

Following gene: 158335500

Centisome position: 36.13

GC content: 50.86

Gene sequence:

>2328_bases
ATGAGATCGTCTGTTCAAGACAATTTTGTGGAAAGCCTGTATTGGTTAAACAAGCTAGAAACAACTCACTCTCTGCGGGT
GGGTGACGCTGCCTTAGGGCTGGCTGAGCTCTATAATGCTCAGGTGCCGGTTCTGCCCAGTGCAGTTATCGGTACAGAAT
CTTTTCAGCAGTTTTGCCGATCGATTGTTTGGTCTTCCCCTTTCTTAAGTGATTTTCCCCATGTAAGTTTGCGCATACGA
GCAGCGCACCCCTTCCAGCTGCAAGCGGTTGCAGAAGAAATTGCCCAAAGGTTTGAGCAAACGGCTTTACCCCCACTGTG
GTTGCAGACCGCTTCAGCAGAATTGCAGAGTCTATCCAACGACTGGCGTCTGTGCCTATCCTTGACCTTGCCAAAACCTT
GGGCTCAATTTACCCCCCTCCTGACGGAGATGTTGCCAGTTCAATACTGTTCATTGACCGGCATTGGCACAGCCATCAAA
CAAGCCTGGAACAGTACTTTTACAGCTCAGACACTCCTGCTCATGCAGGAACGAGCGATTCCCATTGATCAATTACGCGT
AGCGCTACTATTACAGCCCGTCACGTCAGCCCAGGCATCTGGATTTTTAACCGTCACAGAAACCTACATCCAGGTGCAAG
CGACCCATGGTCTGAGCAAAAGTCTGATGATGGGCGAAATCTTACCTGAAACCTATACCTATGACCGCCAGGAAAAGCGG
TGGCACCGCTATCCTGGCTCGATCACGAGTGCCTACCAAGTTCCTTTTGAAACATCTATCCCAGGAGAATTGGGTTTAGT
ACAGCAGATCCTGACACCCGTTTCAGACTCGTGGGTCCTTGAGCAACTCCAACTAGAGCAATTACTAGATTTAGCCCAGA
AGCTTCCTCCTTTACAGTCTGAGTTACAAGCAGTGGAATGGATATTCTCTGGGAAAGAATCTGAGGAACTCCACATTGCA
GGGTTAAGGCCAGCCCAGCCCTTGCCACCTCTGACTGCCGCTCTTCAGCTCCGAGAAGAACCCGGGTTAGATGATAACTC
TCGGGTAATCGCTGCCGGATTAGCCGCAGCAGTCGGCCAGGTAACAGCCCCGATTCTCGTCTGTGAAGATCCAAGTCAAC
TGCAGCCTAACATCGTGGCCAACCAAATTGTTGTCCTAAAGCAGGTCAGGCCTGTCGACTGGGTTTGGTTACAAAAGGTC
GCAGGCTTAATTTGCACAACAGGCGGACATACCTCTCATGGGGCCATAATGGCCCGCGAATTAGGATTGCCTGCAGTGGT
CGGCATTGCTGGTGCATTAGAGACGTTCCAGTCAGGGCAAATGGTGTTGCTAGATGGGAATCAAGGCCAGGTCTTGGCCG
CCCCCTCCTCTAGCTCACCAGGCAGGGCATCTTTATCGACTCCTGCGAAACCCGCCCTCGATCTCGATCTGCAAACTACC
CAGTTATGGGTCAATCTGAGCCAGTCTCAACGGCTAGAGCGGGCTCTACATCTGCCAGTCCATGGCGTCGGCTTATTACG
ATCTGAATGGTTTCTGCTCGATATTTGCCAAGGACAATTACCTGAGGTTTGGATCCAACAACAGGGGGCTGACGCCTTTG
TTAGCCATTTGAGCCACCATTTACTGTCGTTTGTCCAAGCCTGGCATCCTCGGCCTGTGCTGTATCGGTCCCTCGACTGT
AAACAAGATGCTGCTCACTCGTTACAACCCGCCAACTCGATATTGGGACTGCGGGGGGGAGCGCAATATATGTATGACTC
AACCCTATTTGATCTGGAATTGCAGGCACTGGCGAAGCTTTGTCACCAAGGCTGTCAAAATATACGCTTGATCTTGCCAT
TCATTCGCTCGGTCGAGGAATTTGTCTTCTGTCGAGATCGGCTTCACGCTGCTCATCTCCTGGAGCATCTGCAGATTTGG
ATCATGGCAGAGGTGCCTTCAGTGCTTTTTCAACTGTCTGAGTATGTAGCAGCAGGGGTGCAGGGGATTGCGATTGGCAC
CAATGATCTCACTCAACTGATCTTAGGTGTTCACCGAGAGAATACCTATTTGACGGAGCGATATACCGCGAATCATCCGG
CTGTGGTTGCCGCCCTCTCTCAATTGGTCAAGACTGCTAAATCCTTAGGAATTCCCTGCTCTATCTGTGGCCAAGCTCCG
GTTCAACATCCTCAGCTGATTCACCAGTTTGTTGCATGGGGAGTGACCGCTATCTCTGTAGATTTGTCGGCAGTACCCGC
AACTCAGATTGCGATCGCAACGGCCGAAGCACAGCTTAAATCTAAGACTGCTTCTACTTCTGCATCTTCAACTATCCAAA
ACGGTTGA

Upstream 100 bases:

>100_bases
AGTAGAGTGTCTTTCATATTACGATCTGGAACTCAAAATATCGATATATTGCTTTATGGATTTAGTTATATCTCATTGAC
CAACAGGTGTTTGCACTAGG

Downstream 100 bases:

>100_bases
TGGGCTGAGATAATGACGATGGGCGTCCCTCGATACGTCTTCGTTCATTTGTCTTTCTCTAAGCCATGCAAACTTACCAC
TACGCCCTTGCCAGTCAGCG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 775; Mature: 775

Protein sequence:

>775_residues
MRSSVQDNFVESLYWLNKLETTHSLRVGDAALGLAELYNAQVPVLPSAVIGTESFQQFCRSIVWSSPFLSDFPHVSLRIR
AAHPFQLQAVAEEIAQRFEQTALPPLWLQTASAELQSLSNDWRLCLSLTLPKPWAQFTPLLTEMLPVQYCSLTGIGTAIK
QAWNSTFTAQTLLLMQERAIPIDQLRVALLLQPVTSAQASGFLTVTETYIQVQATHGLSKSLMMGEILPETYTYDRQEKR
WHRYPGSITSAYQVPFETSIPGELGLVQQILTPVSDSWVLEQLQLEQLLDLAQKLPPLQSELQAVEWIFSGKESEELHIA
GLRPAQPLPPLTAALQLREEPGLDDNSRVIAAGLAAAVGQVTAPILVCEDPSQLQPNIVANQIVVLKQVRPVDWVWLQKV
AGLICTTGGHTSHGAIMARELGLPAVVGIAGALETFQSGQMVLLDGNQGQVLAAPSSSSPGRASLSTPAKPALDLDLQTT
QLWVNLSQSQRLERALHLPVHGVGLLRSEWFLLDICQGQLPEVWIQQQGADAFVSHLSHHLLSFVQAWHPRPVLYRSLDC
KQDAAHSLQPANSILGLRGGAQYMYDSTLFDLELQALAKLCHQGCQNIRLILPFIRSVEEFVFCRDRLHAAHLLEHLQIW
IMAEVPSVLFQLSEYVAAGVQGIAIGTNDLTQLILGVHRENTYLTERYTANHPAVVAALSQLVKTAKSLGIPCSICGQAP
VQHPQLIHQFVAWGVTAISVDLSAVPATQIAIATAEAQLKSKTASTSASSTIQNG

Sequences:

>Translated_775_residues
MRSSVQDNFVESLYWLNKLETTHSLRVGDAALGLAELYNAQVPVLPSAVIGTESFQQFCRSIVWSSPFLSDFPHVSLRIR
AAHPFQLQAVAEEIAQRFEQTALPPLWLQTASAELQSLSNDWRLCLSLTLPKPWAQFTPLLTEMLPVQYCSLTGIGTAIK
QAWNSTFTAQTLLLMQERAIPIDQLRVALLLQPVTSAQASGFLTVTETYIQVQATHGLSKSLMMGEILPETYTYDRQEKR
WHRYPGSITSAYQVPFETSIPGELGLVQQILTPVSDSWVLEQLQLEQLLDLAQKLPPLQSELQAVEWIFSGKESEELHIA
GLRPAQPLPPLTAALQLREEPGLDDNSRVIAAGLAAAVGQVTAPILVCEDPSQLQPNIVANQIVVLKQVRPVDWVWLQKV
AGLICTTGGHTSHGAIMARELGLPAVVGIAGALETFQSGQMVLLDGNQGQVLAAPSSSSPGRASLSTPAKPALDLDLQTT
QLWVNLSQSQRLERALHLPVHGVGLLRSEWFLLDICQGQLPEVWIQQQGADAFVSHLSHHLLSFVQAWHPRPVLYRSLDC
KQDAAHSLQPANSILGLRGGAQYMYDSTLFDLELQALAKLCHQGCQNIRLILPFIRSVEEFVFCRDRLHAAHLLEHLQIW
IMAEVPSVLFQLSEYVAAGVQGIAIGTNDLTQLILGVHRENTYLTERYTANHPAVVAALSQLVKTAKSLGIPCSICGQAP
VQHPQLIHQFVAWGVTAISVDLSAVPATQIAIATAEAQLKSKTASTSASSTIQNG
>Mature_775_residues
MRSSVQDNFVESLYWLNKLETTHSLRVGDAALGLAELYNAQVPVLPSAVIGTESFQQFCRSIVWSSPFLSDFPHVSLRIR
AAHPFQLQAVAEEIAQRFEQTALPPLWLQTASAELQSLSNDWRLCLSLTLPKPWAQFTPLLTEMLPVQYCSLTGIGTAIK
QAWNSTFTAQTLLLMQERAIPIDQLRVALLLQPVTSAQASGFLTVTETYIQVQATHGLSKSLMMGEILPETYTYDRQEKR
WHRYPGSITSAYQVPFETSIPGELGLVQQILTPVSDSWVLEQLQLEQLLDLAQKLPPLQSELQAVEWIFSGKESEELHIA
GLRPAQPLPPLTAALQLREEPGLDDNSRVIAAGLAAAVGQVTAPILVCEDPSQLQPNIVANQIVVLKQVRPVDWVWLQKV
AGLICTTGGHTSHGAIMARELGLPAVVGIAGALETFQSGQMVLLDGNQGQVLAAPSSSSPGRASLSTPAKPALDLDLQTT
QLWVNLSQSQRLERALHLPVHGVGLLRSEWFLLDICQGQLPEVWIQQQGADAFVSHLSHHLLSFVQAWHPRPVLYRSLDC
KQDAAHSLQPANSILGLRGGAQYMYDSTLFDLELQALAKLCHQGCQNIRLILPFIRSVEEFVFCRDRLHAAHLLEHLQIW
IMAEVPSVLFQLSEYVAAGVQGIAIGTNDLTQLILGVHRENTYLTERYTANHPAVVAALSQLVKTAKSLGIPCSICGQAP
VQHPQLIHQFVAWGVTAISVDLSAVPATQIAIATAEAQLKSKTASTSASSTIQNG

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=518, Percent_Identity=28.5714285714286, Blast_Score=162, Evalue=9e-41,
Organism=Escherichia coli, GI1789193, Length=374, Percent_Identity=27.2727272727273, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI48994992, Length=368, Percent_Identity=25.8152173913043, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1788756, Length=348, Percent_Identity=26.7241379310345, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1788726, Length=370, Percent_Identity=24.3243243243243, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 84860; Mature: 84860

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSVQDNFVESLYWLNKLETTHSLRVGDAALGLAELYNAQVPVLPSAVIGTESFQQFCR
CCCCHHHHHHHHHHHHHHHCCHHCEECCHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHH
SIVWSSPFLSDFPHVSLRIRAAHPFQLQAVAEEIAQRFEQTALPPLWLQTASAELQSLSN
HHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
DWRLCLSLTLPKPWAQFTPLLTEMLPVQYCSLTGIGTAIKQAWNSTFTAQTLLLMQERAI
CEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
PIDQLRVALLLQPVTSAQASGFLTVTETYIQVQATHGLSKSLMMGEILPETYTYDRQEKR
CHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHH
WHRYPGSITSAYQVPFETSIPGELGLVQQILTPVSDSWVLEQLQLEQLLDLAQKLPPLQS
HHCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
ELQAVEWIFSGKESEELHIAGLRPAQPLPPLTAALQLREEPGLDDNSRVIAAGLAAAVGQ
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VTAPILVCEDPSQLQPNIVANQIVVLKQVRPVDWVWLQKVAGLICTTGGHTSHGAIMARE
HHCCEEEECCCCCCCCCHHHHHHHEEHHCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHH
LGLPAVVGIAGALETFQSGQMVLLDGNQGQVLAAPSSSSPGRASLSTPAKPALDLDLQTT
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCEECCCEEE
QLWVNLSQSQRLERALHLPVHGVGLLRSEWFLLDICQGQLPEVWIQQQGADAFVSHLSHH
HEEEECHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCCHHHHHCCCHHHHHHHHHHH
LLSFVQAWHPRPVLYRSLDCKQDAAHSLQPANSILGLRGGAQYMYDSTLFDLELQALAKL
HHHHHHHCCCCCHHEECCCCCHHHHHCCCCCHHHHCCCCCCCHHHCCHHHHHHHHHHHHH
CHQGCQNIRLILPFIRSVEEFVFCRDRLHAAHLLEHLQIWIMAEVPSVLFQLSEYVAAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGIAIGTNDLTQLILGVHRENTYLTERYTANHPAVVAALSQLVKTAKSLGIPCSICGQAP
CEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCC
VQHPQLIHQFVAWGVTAISVDLSAVPATQIAIATAEAQLKSKTASTSASSTIQNG
CCCHHHHHHHHHHCHHEEEEECCCCCCHHEEEEHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MRSSVQDNFVESLYWLNKLETTHSLRVGDAALGLAELYNAQVPVLPSAVIGTESFQQFCR
CCCCHHHHHHHHHHHHHHHCCHHCEECCHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHH
SIVWSSPFLSDFPHVSLRIRAAHPFQLQAVAEEIAQRFEQTALPPLWLQTASAELQSLSN
HHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
DWRLCLSLTLPKPWAQFTPLLTEMLPVQYCSLTGIGTAIKQAWNSTFTAQTLLLMQERAI
CEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
PIDQLRVALLLQPVTSAQASGFLTVTETYIQVQATHGLSKSLMMGEILPETYTYDRQEKR
CHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHH
WHRYPGSITSAYQVPFETSIPGELGLVQQILTPVSDSWVLEQLQLEQLLDLAQKLPPLQS
HHCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
ELQAVEWIFSGKESEELHIAGLRPAQPLPPLTAALQLREEPGLDDNSRVIAAGLAAAVGQ
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VTAPILVCEDPSQLQPNIVANQIVVLKQVRPVDWVWLQKVAGLICTTGGHTSHGAIMARE
HHCCEEEECCCCCCCCCHHHHHHHEEHHCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHH
LGLPAVVGIAGALETFQSGQMVLLDGNQGQVLAAPSSSSPGRASLSTPAKPALDLDLQTT
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCEECCCEEE
QLWVNLSQSQRLERALHLPVHGVGLLRSEWFLLDICQGQLPEVWIQQQGADAFVSHLSHH
HEEEECHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCCHHHHHCCCHHHHHHHHHHH
LLSFVQAWHPRPVLYRSLDCKQDAAHSLQPANSILGLRGGAQYMYDSTLFDLELQALAKL
HHHHHHHCCCCCHHEECCCCCHHHHHCCCCCHHHHCCCCCCCHHHCCHHHHHHHHHHHHH
CHQGCQNIRLILPFIRSVEEFVFCRDRLHAAHLLEHLQIWIMAEVPSVLFQLSEYVAAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGIAIGTNDLTQLILGVHRENTYLTERYTANHPAVVAALSQLVKTAKSLGIPCSICGQAP
CEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCC
VQHPQLIHQFVAWGVTAISVDLSAVPATQIAIATAEAQLKSKTASTSASSTIQNG
CCCHHHHHHHHHHCHHEEEEECCCCCCHHEEEEHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]