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The map label for this gene is pucG [H]

Identifier: 158335418

GI number: 158335418

Start: 2265295

End: 2266440

Strand: Reverse

Name: pucG [H]

Synonym: AM1_2262

Alternate gene names: 158335418

Gene position: 2266440-2265295 (Counterclockwise)

Preceding gene: 158335427

Following gene: 158335414

Centisome position: 34.85

GC content: 52.09

Gene sequence:

>1146_bases
ATGACCAACGTTGCCATCAATGAGACCCATCGTCAGCAAACTGCTCAGCTAGACGTTCCCCCCCGCCTATTAATGGGGCC
GGGGCCGTCCAACGCCCATCCCAGAGTTTTACAAGCGTTGGGAATGCGCCAAGTAGGGCACTTAGATCCTTGCTTTATCG
AGCTGATGAACGAAGTTCAGTCTCTGCTTAAATATGCTTGGCAAACGGAGAACCCGACGACGATTCCGGTGAGCGCGACG
GGTAGCGCGGCTATGGAAGCCACTTTGGCCAATACCGTCGAACCAGGAGATGTGGTTTTAGTGGGCGTCAATGGCTATTT
CGGTCATCGAATGATGGATATGGCTGGGCGTTATGGGGCGGATGTGCGTCGCATCGATAAACCCTGGGGAGAAGTCTTTT
CCTTAGATGAATTGAAAGCTGGCCTGGAAGAGCATCGTCCCAAGGTTTTGGGGCTGGTTCATGCGGAAACATCTACGGGC
GCCTGCCAACCCTTAGAAGGTGTGGGTGACTTGTGTCGAGAATATGATTGTTTGCTGCTGGCAGATACGGTGACTAGCTT
AGGGGGCTTACCGCTGTTTTTGGATGAATGGGGCGTGGATATGGCCTATAGCGGTAGCCAAAAATGCCTGAGCTGCCCAC
CGGGAATTAGTCCGTTCACCTTAGGGGCTCGCGCGGTGGAGAAGCTCAATCAGCGAGAGAAAAAAGTGCCCAACTGGTAT
TTAGATATGTCGATGGTGGGCAAATATTGGGGGTCGGAGCGAACCTATCACCATACAGCTCCCATTAATATGATCTACGG
CTTTCGGGAAGCCCTCCGTTTGATTGCCGAAGAAGGTCTCGCGGAACGCTGGGCGCGACATCAAACCAATGCCGAGTTGA
TGTGGGAAGGGTTAGCTTCCCTGGGCATGGAGTGCTATGTGGAACGCCAGCATCGATTGCCTTCGTTAATTACGGTGCGG
ATTCCAGATGGGGTGGATGGTAAAGCTATCACCCAGCAATTCCTGAAAGACTTTAATATCGAAATTGGGAATGGTTTAGG
AGAACTGGCGGGCAAGGCCTGGCGAATTGGACTGATGGGCTTTAACAGCCGCCCTGAAAATGTCCATATGGTTTTGGATG
CCTTTAAGTACACCTTGAATCGCTAG

Upstream 100 bases:

>100_bases
CCCTGAATCGAGATGCCGCCCTGTGGGACCATCTGCGACCATCATCTGGATCAGCCTGGTAACCTAACTGACCTTCCTCT
ATCTATTCCGGCCCATTCCT

Downstream 100 bases:

>100_bases
TTTTCTGGATCACAGCGGATTTCAAGTAAGAACCCATCTGGATCATAAAAATAAATGCCCCGCCCGGTGGGTCGGGAGAC
TGGACCTTGTGCGATCGCAA

Product: class V aminotransferase

Products: pyruvate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTNVAINETHRQQTAQLDVPPRLLMGPGPSNAHPRVLQALGMRQVGHLDPCFIELMNEVQSLLKYAWQTENPTTIPVSAT
GSAAMEATLANTVEPGDVVLVGVNGYFGHRMMDMAGRYGADVRRIDKPWGEVFSLDELKAGLEEHRPKVLGLVHAETSTG
ACQPLEGVGDLCREYDCLLLADTVTSLGGLPLFLDEWGVDMAYSGSQKCLSCPPGISPFTLGARAVEKLNQREKKVPNWY
LDMSMVGKYWGSERTYHHTAPINMIYGFREALRLIAEEGLAERWARHQTNAELMWEGLASLGMECYVERQHRLPSLITVR
IPDGVDGKAITQQFLKDFNIEIGNGLGELAGKAWRIGLMGFNSRPENVHMVLDAFKYTLNR

Sequences:

>Translated_381_residues
MTNVAINETHRQQTAQLDVPPRLLMGPGPSNAHPRVLQALGMRQVGHLDPCFIELMNEVQSLLKYAWQTENPTTIPVSAT
GSAAMEATLANTVEPGDVVLVGVNGYFGHRMMDMAGRYGADVRRIDKPWGEVFSLDELKAGLEEHRPKVLGLVHAETSTG
ACQPLEGVGDLCREYDCLLLADTVTSLGGLPLFLDEWGVDMAYSGSQKCLSCPPGISPFTLGARAVEKLNQREKKVPNWY
LDMSMVGKYWGSERTYHHTAPINMIYGFREALRLIAEEGLAERWARHQTNAELMWEGLASLGMECYVERQHRLPSLITVR
IPDGVDGKAITQQFLKDFNIEIGNGLGELAGKAWRIGLMGFNSRPENVHMVLDAFKYTLNR
>Mature_380_residues
TNVAINETHRQQTAQLDVPPRLLMGPGPSNAHPRVLQALGMRQVGHLDPCFIELMNEVQSLLKYAWQTENPTTIPVSATG
SAAMEATLANTVEPGDVVLVGVNGYFGHRMMDMAGRYGADVRRIDKPWGEVFSLDELKAGLEEHRPKVLGLVHAETSTGA
CQPLEGVGDLCREYDCLLLADTVTSLGGLPLFLDEWGVDMAYSGSQKCLSCPPGISPFTLGARAVEKLNQREKKVPNWYL
DMSMVGKYWGSERTYHHTAPINMIYGFREALRLIAEEGLAERWARHQTNAELMWEGLASLGMECYVERQHRLPSLITVRI
PDGVDGKAITQQFLKDFNIEIGNGLGELAGKAWRIGLMGFNSRPENVHMVLDAFKYTLNR

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=368, Percent_Identity=42.6630434782609, Blast_Score=318, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17536281, Length=365, Percent_Identity=39.4520547945205, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6321079, Length=376, Percent_Identity=26.063829787234, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17530823, Length=362, Percent_Identity=42.5414364640884, Blast_Score=313, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.44

Molecular weight: Translated: 42280; Mature: 42149

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVAINETHRQQTAQLDVPPRLLMGPGPSNAHPRVLQALGMRQVGHLDPCFIELMNEVQ
CCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHCHHHCCCCCHHHHHHHHHHH
SLLKYAWQTENPTTIPVSATGSAAMEATLANTVEPGDVVLVGVNGYFGHRMMDMAGRYGA
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCC
DVRRIDKPWGEVFSLDELKAGLEEHRPKVLGLVHAETSTGACQPLEGVGDLCREYDCLLL
HHHHHHCCHHHHCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEE
ADTVTSLGGLPLFLDEWGVDMAYSGSQKCLSCPPGISPFTLGARAVEKLNQREKKVPNWY
EHHHHHHCCCEEEEHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LDMSMVGKYWGSERTYHHTAPINMIYGFREALRLIAEEGLAERWARHQTNAELMWEGLAS
ECHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LGMECYVERQHRLPSLITVRIPDGVDGKAITQQFLKDFNIEIGNGLGELAGKAWRIGLMG
CCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCEECCCHHHHCCCEEEEEEEE
FNSRPENVHMVLDAFKYTLNR
CCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNVAINETHRQQTAQLDVPPRLLMGPGPSNAHPRVLQALGMRQVGHLDPCFIELMNEVQ
CCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHCHHHCCCCCHHHHHHHHHHH
SLLKYAWQTENPTTIPVSATGSAAMEATLANTVEPGDVVLVGVNGYFGHRMMDMAGRYGA
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCC
DVRRIDKPWGEVFSLDELKAGLEEHRPKVLGLVHAETSTGACQPLEGVGDLCREYDCLLL
HHHHHHCCHHHHCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEE
ADTVTSLGGLPLFLDEWGVDMAYSGSQKCLSCPPGISPFTLGARAVEKLNQREKKVPNWY
EHHHHHHCCCEEEEHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LDMSMVGKYWGSERTYHHTAPINMIYGFREALRLIAEEGLAERWARHQTNAELMWEGLAS
ECHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LGMECYVERQHRLPSLITVRIPDGVDGKAITQQFLKDFNIEIGNGLGELAGKAWRIGLMG
CCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCEECCCHHHHCCCEEEEEEEE
FNSRPENVHMVLDAFKYTLNR
CCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-alanine; glyoxylate

Specific reaction: L-alanine + glyoxylate = pyruvate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]