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The map label for this gene is nitA [H]

Identifier: 158335383

GI number: 158335383

Start: 2237208

End: 2238206

Strand: Direct

Name: nitA [H]

Synonym: AM1_2228

Alternate gene names: 158335383

Gene position: 2237208-2238206 (Clockwise)

Preceding gene: 158335382

Following gene: 158335384

Centisome position: 34.4

GC content: 56.96

Gene sequence:

>999_bases
ATGGATCACGAAAAAGCAGTACGAGCTGCTGCGGTGCAGATCAGTCCGGTCTTGTTCAGTCGAGAAGGGACCACGGAGAA
GGTTTTGGATGCGATCGCATCTGCGGCCCAAGAAGAGGTGCAATTGATTGTCTTCCCGGAAACCTTTGTCCCCTACTATC
CCTATTTCTCCTTTGTTCAGCCGCCCGTGGTGATGGGGAAGGCCCATATGCAGCTGTATGAAGAAGCAGTGACTGTGCCG
GGAGCCGTGGTGGATGCCATCAGCCGAGCCGCTCGCTCCTACGAAATGGTGGTGGTTCTGGGGGTGAATGAGCGGGAGGG
CGGTTCCCTCTATAACACCCAACTGATCTTTGATGCCGATGGCACCTTGGGGCTGAAGCGACGCAAGATTACCCCTACCT
ACCACGAACGGATGGTCTGGGGACAAGGAGATGGGGCTGGACTCGCCGTCCTAGAGACGGCAGTGGGTCGGCTAGGGGCC
TTGGCCTGCTGGGAGCATTACAATCCCCTAGCTCGCTATGCCCTGATGGCTCAGCAGGAAGAAATTCACTGTGGGCAGTT
CCCCGGATCCATGGTGGGGCAAATCTTTGCCGACCAAATGGAGGTGACCATGCGTCACCATGCTCTAGAGTCGGGCTGTT
TCGTGGTCAATGCCACGGGCTGGCTTACCCCAGAGCAAAAGCAGCAAATTACCGCCGATGAAAAACTGCAAAAAGTCCTC
AGTGGCGGCTGCTATACCGCCATTATTAGCCCCGAGGGGGTGCCCCTCTGCGACCCCGTGACCGAGGGAGAAGGGATGGC
CATTGCCGATCTCGATTTCTCCCTGATTACCAAACGCAAACGAATGATGGACTCCGTAGGCCATTATTCCCGCCCCGATT
TGCTGCAGCTGCAGGTCAACCAACAAGCGTGGGCCGTAATGCAATCGGCTCCCACCGTACCGCTCCCAGAGCAGGATTCT
CCCTTATCCAGTGAGCCCATCCCTCACCCCGTCGATTAA

Upstream 100 bases:

>100_bases
GGATGTCTTGGATATTGTCTTGGTGCACCGCAAAGCAGGGGCCTTTATTCTCGATACCTGGACTATGTAATCGTTGGGCC
AGCCCCTTGAGGTCATCGTT

Downstream 100 bases:

>100_bases
CCCAAGATTATGGACAAACAACATCTAATTACTGAACTGCAATCGAACGGACTGCAGGTGATTGCCCCCAGTATTGGCGT
AGCGGGTCGTCGGGGTGGGG

Product: nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDHEKAVRAAAVQISPVLFSREGTTEKVLDAIASAAQEEVQLIVFPETFVPYYPYFSFVQPPVVMGKAHMQLYEEAVTVP
GAVVDAISRAARSYEMVVVLGVNEREGGSLYNTQLIFDADGTLGLKRRKITPTYHERMVWGQGDGAGLAVLETAVGRLGA
LACWEHYNPLARYALMAQQEEIHCGQFPGSMVGQIFADQMEVTMRHHALESGCFVVNATGWLTPEQKQQITADEKLQKVL
SGGCYTAIISPEGVPLCDPVTEGEGMAIADLDFSLITKRKRMMDSVGHYSRPDLLQLQVNQQAWAVMQSAPTVPLPEQDS
PLSSEPIPHPVD

Sequences:

>Translated_332_residues
MDHEKAVRAAAVQISPVLFSREGTTEKVLDAIASAAQEEVQLIVFPETFVPYYPYFSFVQPPVVMGKAHMQLYEEAVTVP
GAVVDAISRAARSYEMVVVLGVNEREGGSLYNTQLIFDADGTLGLKRRKITPTYHERMVWGQGDGAGLAVLETAVGRLGA
LACWEHYNPLARYALMAQQEEIHCGQFPGSMVGQIFADQMEVTMRHHALESGCFVVNATGWLTPEQKQQITADEKLQKVL
SGGCYTAIISPEGVPLCDPVTEGEGMAIADLDFSLITKRKRMMDSVGHYSRPDLLQLQVNQQAWAVMQSAPTVPLPEQDS
PLSSEPIPHPVD
>Mature_332_residues
MDHEKAVRAAAVQISPVLFSREGTTEKVLDAIASAAQEEVQLIVFPETFVPYYPYFSFVQPPVVMGKAHMQLYEEAVTVP
GAVVDAISRAARSYEMVVVLGVNEREGGSLYNTQLIFDADGTLGLKRRKITPTYHERMVWGQGDGAGLAVLETAVGRLGA
LACWEHYNPLARYALMAQQEEIHCGQFPGSMVGQIFADQMEVTMRHHALESGCFVVNATGWLTPEQKQQITADEKLQKVL
SGGCYTAIISPEGVPLCDPVTEGEGMAIADLDFSLITKRKRMMDSVGHYSRPDLLQLQVNQQAWAVMQSAPTVPLPEQDS
PLSSEPIPHPVD

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=313, Percent_Identity=33.5463258785943, Blast_Score=157, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6322027, Length=185, Percent_Identity=32.4324324324324, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 36403; Mature: 36403

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHEKAVRAAAVQISPVLFSREGTTEKVLDAIASAAQEEVQLIVFPETFVPYYPYFSFVQ
CCHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEECHHHCCCCCCHHHCC
PPVVMGKAHMQLYEEAVTVPGAVVDAISRAARSYEMVVVLGVNEREGGSLYNTQLIFDAD
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEEEEEEECC
GTLGLKRRKITPTYHERMVWGQGDGAGLAVLETAVGRLGALACWEHYNPLARYALMAQQE
CCCCCEECCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
EIHCGQFPGSMVGQIFADQMEVTMRHHALESGCFVVNATGWLTPEQKQQITADEKLQKVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCHHHHHHHHH
SGGCYTAIISPEGVPLCDPVTEGEGMAIADLDFSLITKRKRMMDSVGHYSRPDLLQLQVN
CCCCEEEEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEEC
QQAWAVMQSAPTVPLPEQDSPLSSEPIPHPVD
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDHEKAVRAAAVQISPVLFSREGTTEKVLDAIASAAQEEVQLIVFPETFVPYYPYFSFVQ
CCHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHCEEEEEECHHHCCCCCCHHHCC
PPVVMGKAHMQLYEEAVTVPGAVVDAISRAARSYEMVVVLGVNEREGGSLYNTQLIFDAD
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEEEEEEECC
GTLGLKRRKITPTYHERMVWGQGDGAGLAVLETAVGRLGALACWEHYNPLARYALMAQQE
CCCCCEECCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
EIHCGQFPGSMVGQIFADQMEVTMRHHALESGCFVVNATGWLTPEQKQQITADEKLQKVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCHHHHHHHHH
SGGCYTAIISPEGVPLCDPVTEGEGMAIADLDFSLITKRKRMMDSVGHYSRPDLLQLQVN
CCCCEEEEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEEC
QQAWAVMQSAPTVPLPEQDSPLSSEPIPHPVD
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]