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The map label for this gene is fixJ [H]

Identifier: 158335372

GI number: 158335372

Start: 2223742

End: 2224425

Strand: Reverse

Name: fixJ [H]

Synonym: AM1_2217

Alternate gene names: 158335372

Gene position: 2224425-2223742 (Counterclockwise)

Preceding gene: 158335376

Following gene: 158335371

Centisome position: 34.2

GC content: 51.9

Gene sequence:

>684_bases
ATGACCGGGCATTTACTATTGGTAGACGATGAGCCAGGATTGCGGGAAGCCGTGCAAGCCTACCTCGAAGACAGCGGCTT
TACCGTCGATGTTGCTAGTAATGCCAAAGCTGGATGGGAGCTGCTGCAACAGCGCATTCCCGACCTGGTGATTTCCGATA
TTATGATGCCCGAAGTCGATGGCTACCAATTCCTAGCCCAACTGCGGGAAGATCCTCGGTTTATGCGATTGCCCATCGTA
TTTTTAACCGCCCGTGGCATGACCAAAGATCGGATTGAAGGCTATAACGCTGGAGTGGATGCCTATCTCTCCAAACCCTT
TGACCCGGACGAACTGGTCGCCATCGTCACCAATCTGTTGACCCGTAAAGAAGCCAAAGACGCTAACGCCACCACTGATA
TTGCCGATCTAGCCAATCAGGTGGCCGAAATTCGAGCCATCTTGATGAACCGCGCTCCTTTACCCACCCAAAATACCCCC
ACCGTAAAAATTGACTTTACCCCCCGCGAACAAAGTGTCTTGGACCTGGTGGCGGTCGGGCTCATGAACAAAGAGATTGC
CCGTCGCTTAGAAACCAGCGTCCGCAACGTCGAAAAATATGTCAGCCGACTTTTTAGTAAAACGGGCACCAATAGCCGGA
CAGAACTGGTACGCTTTGCCTTGGAGAACGGTCTGACACAGTAA

Upstream 100 bases:

>100_bases
GCATCGGCCTACCTCAATCCCTCATTCGGCAATTCACCCCTCATACTGACAGATTCGGGCGGTAAACTGAAGCTAGTAGT
ACTCATTAGACGGAAATTCT

Downstream 100 bases:

>100_bases
CTCTTATGCATACCATTTGGCAGACATTGACCTTGAACAACATCCAATCCCTGCAATGGCGACAAGGCAGTCTGTTCTGG
CGACTGCTTGCCTTCCTTCG

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTGHLLLVDDEPGLREAVQAYLEDSGFTVDVASNAKAGWELLQQRIPDLVISDIMMPEVDGYQFLAQLREDPRFMRLPIV
FLTARGMTKDRIEGYNAGVDAYLSKPFDPDELVAIVTNLLTRKEAKDANATTDIADLANQVAEIRAILMNRAPLPTQNTP
TVKIDFTPREQSVLDLVAVGLMNKEIARRLETSVRNVEKYVSRLFSKTGTNSRTELVRFALENGLTQ

Sequences:

>Translated_227_residues
MTGHLLLVDDEPGLREAVQAYLEDSGFTVDVASNAKAGWELLQQRIPDLVISDIMMPEVDGYQFLAQLREDPRFMRLPIV
FLTARGMTKDRIEGYNAGVDAYLSKPFDPDELVAIVTNLLTRKEAKDANATTDIADLANQVAEIRAILMNRAPLPTQNTP
TVKIDFTPREQSVLDLVAVGLMNKEIARRLETSVRNVEKYVSRLFSKTGTNSRTELVRFALENGLTQ
>Mature_226_residues
TGHLLLVDDEPGLREAVQAYLEDSGFTVDVASNAKAGWELLQQRIPDLVISDIMMPEVDGYQFLAQLREDPRFMRLPIVF
LTARGMTKDRIEGYNAGVDAYLSKPFDPDELVAIVTNLLTRKEAKDANATTDIADLANQVAEIRAILMNRAPLPTQNTPT
VKIDFTPREQSVLDLVAVGLMNKEIARRLETSVRNVEKYVSRLFSKTGTNSRTELVRFALENGLTQ

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=37.7049180327869, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786784, Length=133, Percent_Identity=39.8496240601504, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1787375, Length=199, Percent_Identity=31.1557788944724, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=37.8151260504202, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI87082012, Length=133, Percent_Identity=35.3383458646617, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787229, Length=122, Percent_Identity=35.2459016393443, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788394, Length=121, Percent_Identity=34.7107438016529, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790860, Length=116, Percent_Identity=35.3448275862069, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786911, Length=124, Percent_Identity=36.2903225806452, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790299, Length=124, Percent_Identity=32.258064516129, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=122, Percent_Identity=33.6065573770492, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788905, Length=118, Percent_Identity=37.2881355932203, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1789402, Length=125, Percent_Identity=33.6, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1790863, Length=121, Percent_Identity=34.7107438016529, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790552, Length=119, Percent_Identity=33.6134453781513, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787473, Length=213, Percent_Identity=31.924882629108, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI2367329, Length=211, Percent_Identity=29.8578199052133, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790437, Length=221, Percent_Identity=25.7918552036199, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25367; Mature: 25236

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGHLLLVDDEPGLREAVQAYLEDSGFTVDVASNAKAGWELLQQRIPDLVISDIMMPEVD
CCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GYQFLAQLREDPRFMRLPIVFLTARGMTKDRIEGYNAGVDAYLSKPFDPDELVAIVTNLL
HHHHHHHHHCCCCEEEHHHHEEECCCCCHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHH
TRKEAKDANATTDIADLANQVAEIRAILMNRAPLPTQNTPTVKIDFTPREQSVLDLVAVG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
LMNKEIARRLETSVRNVEKYVSRLFSKTGTNSRTELVRFALENGLTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TGHLLLVDDEPGLREAVQAYLEDSGFTVDVASNAKAGWELLQQRIPDLVISDIMMPEVD
CCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GYQFLAQLREDPRFMRLPIVFLTARGMTKDRIEGYNAGVDAYLSKPFDPDELVAIVTNLL
HHHHHHHHHCCCCEEEHHHHEEECCCCCHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHH
TRKEAKDANATTDIADLANQVAEIRAILMNRAPLPTQNTPTVKIDFTPREQSVLDLVAVG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
LMNKEIARRLETSVRNVEKYVSRLFSKTGTNSRTELVRFALENGLTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]