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The map label for this gene is yafV [C]

Identifier: 158335288

GI number: 158335288

Start: 2126085

End: 2126897

Strand: Direct

Name: yafV [C]

Synonym: AM1_2131

Alternate gene names: 158335288

Gene position: 2126085-2126897 (Clockwise)

Preceding gene: 158335287

Following gene: 158335289

Centisome position: 32.69

GC content: 49.69

Gene sequence:

>813_bases
ATGAAGCCCTACCTTGCTGCCGCAGTTCAAATGACCAGTTTGCCAGATTTACACAAAAATTTGGCCCAAGCGACGGATTT
AATTGAGCTAGCAGTGCGCCGCGGAGCCGAATTAGTGTGTTTGCCTGAGAATTTTTCTTTTCTTGGAGATGAATCAGCCA
AAATTGAGCAAGCTGAAACCATTGGTTTAGAATCTGAAAAATTTATCAAAACCATGGCTCAGCGCTTCCAAATCACGATC
TTGGGAGGGGGCTACCCTGTACCTGCAGAGGCGGGCAAAGTCTACAATACAGCTCTACTAGTGGCACCAGATGGGAGTGA
ACTAGCTCGATACCATAAAGTCCACCTCTTCGACGTCGAACTCCCGGACGGCAATATTTATCATGAGTCAGGCATTGTGC
TCGCTGGCTCGGAGATTCCCCCCATTTACGCTTCGCCCAAATATGGAAACTTGGGTATGTCCGTTTGCTACGATGTTCGA
TTTCCTGAGTTTTATCGGGCCTTGTCTAAAGCTGGAGCAGACGTACTGTTTGTTCCAGCAGCGTTCACGGCCTTTACGGG
CAAAGATCACTGGCAAGTGCTACTCCAAGCCCGTGCCATTGAGAATACGACTTACGTCATTGCCCCAGCTCAAACTGGTT
TCCACAACTCTCGCCGCCAATCCCATGGCCATGCTCTGATTGTCGATCCCTGGGGAGTGGTCTTAGCAGATGCCAGTATT
GAGCCAGGAGTCGCTGTAGCTGCCATCGAACCCTCCCGTCTGGATCAAGTGCGGCAGCAAATGCCTTGTTTAAACCACCG
CGTATTTGGTTAA

Upstream 100 bases:

>100_bases
TTTTTCGCAAATATGTTCTTTTACGAGGATCTCTTGACGAGGATGGTCCGCAAAGCTTTACGATGAGTTAGTTTATGCCT
TTCATCGTTCGCCACACGCT

Downstream 100 bases:

>100_bases
GTCTTTTACCGTCAATGGTCCTTCATACCTGTATTTAAAGATGCGAAGCAGTCCGCAACGGGTTTGACAGTGTGGCTTAA
TATCTGTACCCCTCTCCCCT

Product: carbon-nitrogen family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKPYLAAAVQMTSLPDLHKNLAQATDLIELAVRRGAELVCLPENFSFLGDESAKIEQAETIGLESEKFIKTMAQRFQITI
LGGGYPVPAEAGKVYNTALLVAPDGSELARYHKVHLFDVELPDGNIYHESGIVLAGSEIPPIYASPKYGNLGMSVCYDVR
FPEFYRALSKAGADVLFVPAAFTAFTGKDHWQVLLQARAIENTTYVIAPAQTGFHNSRRQSHGHALIVDPWGVVLADASI
EPGVAVAAIEPSRLDQVRQQMPCLNHRVFG

Sequences:

>Translated_270_residues
MKPYLAAAVQMTSLPDLHKNLAQATDLIELAVRRGAELVCLPENFSFLGDESAKIEQAETIGLESEKFIKTMAQRFQITI
LGGGYPVPAEAGKVYNTALLVAPDGSELARYHKVHLFDVELPDGNIYHESGIVLAGSEIPPIYASPKYGNLGMSVCYDVR
FPEFYRALSKAGADVLFVPAAFTAFTGKDHWQVLLQARAIENTTYVIAPAQTGFHNSRRQSHGHALIVDPWGVVLADASI
EPGVAVAAIEPSRLDQVRQQMPCLNHRVFG
>Mature_270_residues
MKPYLAAAVQMTSLPDLHKNLAQATDLIELAVRRGAELVCLPENFSFLGDESAKIEQAETIGLESEKFIKTMAQRFQITI
LGGGYPVPAEAGKVYNTALLVAPDGSELARYHKVHLFDVELPDGNIYHESGIVLAGSEIPPIYASPKYGNLGMSVCYDVR
FPEFYRALSKAGADVLFVPAAFTAFTGKDHWQVLLQARAIENTTYVIAPAQTGFHNSRRQSHGHALIVDPWGVVLADASI
EPGVAVAAIEPSRLDQVRQQMPCLNHRVFG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=268, Percent_Identity=38.4328358208955, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI297632348, Length=268, Percent_Identity=38.4328358208955, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI5031947, Length=268, Percent_Identity=38.4328358208955, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI9910460, Length=267, Percent_Identity=32.5842696629214, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI297632346, Length=196, Percent_Identity=34.6938775510204, Blast_Score=114, Evalue=8e-26,
Organism=Escherichia coli, GI1786412, Length=236, Percent_Identity=27.5423728813559, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17556280, Length=267, Percent_Identity=42.3220973782771, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6322335, Length=297, Percent_Identity=34.6801346801347, Blast_Score=174, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6323383, Length=271, Percent_Identity=33.210332103321, Blast_Score=159, Evalue=3e-40,
Organism=Drosophila melanogaster, GI17933642, Length=260, Percent_Identity=38.8461538461538, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21355835, Length=264, Percent_Identity=32.5757575757576, Blast_Score=147, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29460; Mature: 29460

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYLAAAVQMTSLPDLHKNLAQATDLIELAVRRGAELVCLPENFSFLGDESAKIEQAET
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHH
IGLESEKFIKTMAQRFQITILGGGYPVPAEAGKVYNTALLVAPDGSELARYHKVHLFDVE
CCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEEEECCCHHHHHEEEEEEEEEE
LPDGNIYHESGIVLAGSEIPPIYASPKYGNLGMSVCYDVRFPEFYRALSKAGADVLFVPA
CCCCCEEECCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECC
AFTAFTGKDHWQVLLQARAIENTTYVIAPAQTGFHNSRRQSHGHALIVDPWGVVLADASI
EEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEEECCCCEEEECCCC
EPGVAVAAIEPSRLDQVRQQMPCLNHRVFG
CCCEEEEEECHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKPYLAAAVQMTSLPDLHKNLAQATDLIELAVRRGAELVCLPENFSFLGDESAKIEQAET
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHH
IGLESEKFIKTMAQRFQITILGGGYPVPAEAGKVYNTALLVAPDGSELARYHKVHLFDVE
CCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEEEECCCHHHHHEEEEEEEEEE
LPDGNIYHESGIVLAGSEIPPIYASPKYGNLGMSVCYDVRFPEFYRALSKAGADVLFVPA
CCCCCEEECCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECC
AFTAFTGKDHWQVLLQARAIENTTYVIAPAQTGFHNSRRQSHGHALIVDPWGVVLADASI
EEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCEEEECCCCEEEECCCC
EPGVAVAAIEPSRLDQVRQQMPCLNHRVFG
CCCEEEEEECHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]