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The map label for this gene is insG [H]

Identifier: 158335231

GI number: 158335231

Start: 2065371

End: 2066789

Strand: Direct

Name: insG [H]

Synonym: AM1_2074

Alternate gene names: 158335231

Gene position: 2065371-2066789 (Clockwise)

Preceding gene: 158335229

Following gene: 158335234

Centisome position: 31.76

GC content: 50.74

Gene sequence:

>1419_bases
TTGATAGATATAGGATCAAGACTTGCACGCCATAAACTTCTGCCTTTCACTATGTCTGAGTCAGCCGAAATTCTCAAGCA
GCAATTTTCCCAAAGCCTGGGTTTACCGTGGACAGATATCCTTCCAGCCTCAAGGTTAGAGGAACTCCTGAAAGAAGAAG
CATTCTCCTATCGCAACCGGATATATAGCCCCATCGTTACGCTGTGGGCCATGCTTTACCAAGTGCTATCAGCCGATAAA
AGCCTTCGTAACACAGTCAAATGCATCACGACTTGGCTCACAGCGGCTGGCATCCAACCGCCCTCATCTGATACCGGAGC
CTACAGCAAAGCCAGAAGTCGCTTTCCAGAGTCACTGTTGCAACGTTTAATTCCCGAAAGCGCTGAGTGCTTAGCGCAAC
CCCTCTCCCCAGAGCACCTCTGGTGTGGTCGGCCCGTCAAGGTCTACGACGGAACCACCGTGTTGATGGCTGATAGTGCG
GCCAACCAAGCATCATATCCACAACATGGCAATCAAACAGCAGGCTGTGGTTTTCCCATCGCTCGCTTGGTAGTGTTCTT
CTGTTTGGTTACCGGTGCAGTGGCGTCAGCTTGTATTGCCTCCTGGGACACCAGTGAAATTGTCATGAGTCGTTTGCTCT
ATCAAGACCTTGAGGTCGGTGATGTGGTCATGGCGGACCAAGCTTATGGCAGCTATGTTGATCTAGCCATCATTCAACAA
CACAGGGCTGATGGAGTGTTGCGTAAACATCATGCTCGCAAGACTGATTTCCGCAAAGGCAACAAGCATGGCATTGGTGA
TCATCAGGTGACATGGCATAAGCCAGCCCAACGGCCTGAGCACATGAGTGAGCAAGATTTTGCCCTGATTCCTCAAACAT
TGGTCGTTAGAGAGGTGTGTTTGCGCTTATCCCTTAAGGGCTTTCGCGACCAGCACATTATTGTGGTGACGACGCTGCTG
GATGCTCAACGCTACAGCGCTGGGCAACTGACTCGCTTGTATGGCTGGCGTTGGCCAGTGGCGGAAGTCAATCTGCGCCA
TCTCAAAACCACCTTAAAAATGGAGATGCTCAGTGCCAAAACTCCGGATATGGTGCGCAAGGACATTTGGGTACATTTGT
TGGGCTATAACCTACTCAGAAGTCTCATGGAACTTGCGGCACCGCTAGCAGATAATGCTAGAACTCAACTGTCTGTGCAA
GGAGCACGACAACACTTCAATCAGATGCTTGCTTTGTTGGCGACAGCCAACCGTGCGACCAGAAAGCGGTTGTTTACTCA
TCTACTTGAGACCATGGCAGCCGATCTATTACCCTCTCGACCGAATCGGCACGAACCGAGAGTCGTCAAACGCAGACCCA
AATCTTTCCCGCGAATGCGACAACCTCGCTCTGCTCTCAAAGCTAAGCTAGCTGTTTGA

Upstream 100 bases:

>100_bases
GTTTGGCCCGCTCTACCCGCTGCTGCAGCAAATATTGAAAGGGAGAAAGCCCCATAGATCAATGGCACTGACTTAAGAAG
TCAGGAAAAGGCTACAAGTC

Downstream 100 bases:

>100_bases
TTGAGCTTAAGTCAGTGCCATTGCCCCATAGATTGCTTGAACAGACGGCTAAAGTGAAATTGGCTCATGTCTAACAACTG
CGCTAAGTCAGCCAGCTTGA

Product: IS4 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADK
SLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSA
ANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVCLRLSLKGFRDQHIIVVTTLL
DAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHFNQMLALLATANRATRKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKAKLAV

Sequences:

>Translated_472_residues
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADK
SLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSA
ANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVCLRLSLKGFRDQHIIVVTTLL
DAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHFNQMLALLATANRATRKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKAKLAV
>Mature_472_residues
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNRIYSPIVTLWAMLYQVLSADK
SLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLLQRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSA
ANQASYPQHGNQTAGCGFPIARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVCLRLSLKGFRDQHIIVVTTLL
DAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAKTPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQ
GARQHFNQMLALLATANRATRKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKAKLAV

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=357, Percent_Identity=25.4901960784314, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 53300; Mature: 53300

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNR
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHH
IYSPIVTLWAMLYQVLSADKSLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSAANQASYPQHGNQTAGCGFPI
HHHCCCHHHHHHCCCCCCCEECCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
ARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
LRLSLKGFRDQHIIVVTTLLDAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAK
HHHHHCCCCCCCEEEEEEHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
TPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQGARQHFNQMLALLATANRAT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKAKLAV
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHCCHHHHHHHHCCC
>Mature Secondary Structure
MIDIGSRLARHKLLPFTMSESAEILKQQFSQSLGLPWTDILPASRLEELLKEEAFSYRNR
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHH
IYSPIVTLWAMLYQVLSADKSLRNTVKCITTWLTAAGIQPPSSDTGAYSKARSRFPESLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QRLIPESAECLAQPLSPEHLWCGRPVKVYDGTTVLMADSAANQASYPQHGNQTAGCGFPI
HHHCCCHHHHHHCCCCCCCEECCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
ARLVVFFCLVTGAVASACIASWDTSEIVMSRLLYQDLEVGDVVMADQAYGSYVDLAIIQQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
HRADGVLRKHHARKTDFRKGNKHGIGDHQVTWHKPAQRPEHMSEQDFALIPQTLVVREVC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
LRLSLKGFRDQHIIVVTTLLDAQRYSAGQLTRLYGWRWPVAEVNLRHLKTTLKMEMLSAK
HHHHHCCCCCCCEEEEEEHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
TPDMVRKDIWVHLLGYNLLRSLMELAAPLADNARTQLSVQGARQHFNQMLALLATANRAT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRLFTHLLETMAADLLPSRPNRHEPRVVKRRPKSFPRMRQPRSALKAKLAV
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]