Click here to switch to the map view.

The map label for this gene is nuoN

Identifier: 158335201

GI number: 158335201

Start: 2036953

End: 2038518

Strand: Direct

Name: nuoN

Synonym: AM1_2044

Alternate gene names: 158335201

Gene position: 2036953-2038518 (Clockwise)

Preceding gene: 158335195

Following gene: 158335203

Centisome position: 31.32

GC content: 50.13

Gene sequence:

>1566_bases
ATGGACGTTGCCAATCTCGCTAGCCAGCTAAATGCCACCACCATCTTGCCTGAAGGTGTTTTGGTGATCTCGTTAATTCT
TGTTCTCCTTGGAGATATTACCTTTGGTCGTTCATCGGCAAAATGGACCCCATACCTTGCGATCGCAAGTTTGGCAGCCT
GCCTCTATCTGCTCTATACCCAGTGGGATCAAGCGAATCCCATCGGTTTTCTGGGGAGCTTCAATGCCGATAACCTCAGC
ATTGTTTTTAGAGCCATTATTGCCCTGTCGACTCTAGTCACCATCCTGATGTCGGTGCGCTATATCGAACAGTCGGGCAG
TTCCATCGGCGAATTTATGACGGTGCTGTTAACAGCCACTGTTGGAGCGATGTTCCTCTGCGGTGCCGAAGAGCTCGTCA
TGATCTTTATCTCTTTGGAAACGTTGAGTATCGCCTCTTACCTAATGACGGGTTACATGAAGCGCGATTCTCGCTCTAAT
GAAGCCGCTCTGAAATATTTGCTGATTGGTGCAGCCAGTTCTGCCGTTTTTCTCTACGGTCTGTCTTTGCTCTATGGCCT
TTCCGGTGGCAAAACCCATTTAGATGACATTGCCCTGGCCTTAACCGATACTTCCAAGTCCCTGGCTCTCGTTATTTCCC
TAGTATTTGTGATTGCTAGCGTCAGCTTCAAGATTGCGGCAGTCCCCTTCCACCAATGGACCCCCGACGTCTACGAAGGC
TCTCCCACACCGGTCGTTGCTTTTCTCTCTGTCGGGTCCAAAGCAGCCGGATTTGCCCTCGCGATTCGCCTGTTAACCCA
AGCCTTCCCCAACCTAGTGGAGCAGTGGCAATTCATCTTCACGGCCTTAGCCATTCTGAGTATGGTGCTTGGTAACGTGG
TTGCCATTGCCCAAACCAGCATGAAGCGGATGCTCGCTTACTCTTCCATTGGTCAGGCAGGCTTTGTCATGATTGGCCTC
GTCATTGGAACAGAAGCAGGTTATGCCAGCATGGTTTTCTACCTGCTGATTTACCTGTTTATGAACCTGGGCGCATTTAC
TTGCGTGATCCTGTTCTCTCTCCGGACTGGCACCGATGAAATTGGCGATTATGCTGGCCTCTATCTTAAAGATCCGCTCT
TAACCCTAGCCTTGAGCCTCTGTTTGCTCTCCCTAGGAGGGATACCGCCGCTTGCAGGCTTCTTCGGCAAGCTATACCTC
TTCTGGGCCGGTTGGCAAGCCGGAGCCTATGGTTTGGTTCTTCTGGGCTTAATCACTAGTGTGGTCTCCATCTACTACTA
CATTCGAGTGATTAAGATGATGGTGGTCAAAGAGCCTCAAGATATGTCTGAGGTGATCAAAAACTATCCCAGCATTGTTT
GGAAGCCCTTAGGAATGCGTCCCTTACAAGTGGGCTTAGTCCTTACGTTAATTGCGACCTCTTTGGCTGGAGTCCTTTCC
AATCCCTTGCTCACGATTGTAAATAGCTCGGTTGCGGATGTCCCTCGCTTTAAGCAAGCAACCATCCCGACGTCCATCGA
TGTTGCGATTCAGCCCGATACACCAACGATTGCACCTGAGCTATAA

Upstream 100 bases:

>100_bases
TGGCGCTTGCGCGTTAAAATTCTCAACATAATCCTTGCCATTGAACTTTATTGTTAGATGACTGGGCTTATGTCGCCAAA
GACATTACCACTCTTGACTA

Downstream 100 bases:

>100_bases
GCGTTATGCTAATCGGGTGGGGCCAGATACCAAGCCATAGATTTATTAGCAACGCCTAGAAGCAAGCAGATTATCTCTTC
TAGAAACAGCTGCATTAGCT

Product: NAD(P)H-quinone oxidoreductase subunit 2

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit 2; NADH-plastoquinone oxidoreductase subunit 2; NDH-1, subunit 2

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MDVANLASQLNATTILPEGVLVISLILVLLGDITFGRSSAKWTPYLAIASLAACLYLLYTQWDQANPIGFLGSFNADNLS
IVFRAIIALSTLVTILMSVRYIEQSGSSIGEFMTVLLTATVGAMFLCGAEELVMIFISLETLSIASYLMTGYMKRDSRSN
EAALKYLLIGAASSAVFLYGLSLLYGLSGGKTHLDDIALALTDTSKSLALVISLVFVIASVSFKIAAVPFHQWTPDVYEG
SPTPVVAFLSVGSKAAGFALAIRLLTQAFPNLVEQWQFIFTALAILSMVLGNVVAIAQTSMKRMLAYSSIGQAGFVMIGL
VIGTEAGYASMVFYLLIYLFMNLGAFTCVILFSLRTGTDEIGDYAGLYLKDPLLTLALSLCLLSLGGIPPLAGFFGKLYL
FWAGWQAGAYGLVLLGLITSVVSIYYYIRVIKMMVVKEPQDMSEVIKNYPSIVWKPLGMRPLQVGLVLTLIATSLAGVLS
NPLLTIVNSSVADVPRFKQATIPTSIDVAIQPDTPTIAPEL

Sequences:

>Translated_521_residues
MDVANLASQLNATTILPEGVLVISLILVLLGDITFGRSSAKWTPYLAIASLAACLYLLYTQWDQANPIGFLGSFNADNLS
IVFRAIIALSTLVTILMSVRYIEQSGSSIGEFMTVLLTATVGAMFLCGAEELVMIFISLETLSIASYLMTGYMKRDSRSN
EAALKYLLIGAASSAVFLYGLSLLYGLSGGKTHLDDIALALTDTSKSLALVISLVFVIASVSFKIAAVPFHQWTPDVYEG
SPTPVVAFLSVGSKAAGFALAIRLLTQAFPNLVEQWQFIFTALAILSMVLGNVVAIAQTSMKRMLAYSSIGQAGFVMIGL
VIGTEAGYASMVFYLLIYLFMNLGAFTCVILFSLRTGTDEIGDYAGLYLKDPLLTLALSLCLLSLGGIPPLAGFFGKLYL
FWAGWQAGAYGLVLLGLITSVVSIYYYIRVIKMMVVKEPQDMSEVIKNYPSIVWKPLGMRPLQVGLVLTLIATSLAGVLS
NPLLTIVNSSVADVPRFKQATIPTSIDVAIQPDTPTIAPEL
>Mature_521_residues
MDVANLASQLNATTILPEGVLVISLILVLLGDITFGRSSAKWTPYLAIASLAACLYLLYTQWDQANPIGFLGSFNADNLS
IVFRAIIALSTLVTILMSVRYIEQSGSSIGEFMTVLLTATVGAMFLCGAEELVMIFISLETLSIASYLMTGYMKRDSRSN
EAALKYLLIGAASSAVFLYGLSLLYGLSGGKTHLDDIALALTDTSKSLALVISLVFVIASVSFKIAAVPFHQWTPDVYEG
SPTPVVAFLSVGSKAAGFALAIRLLTQAFPNLVEQWQFIFTALAILSMVLGNVVAIAQTSMKRMLAYSSIGQAGFVMIGL
VIGTEAGYASMVFYLLIYLFMNLGAFTCVILFSLRTGTDEIGDYAGLYLKDPLLTLALSLCLLSLGGIPPLAGFFGKLYL
FWAGWQAGAYGLVLLGLITSVVSIYYYIRVIKMMVVKEPQDMSEVIKNYPSIVWKPLGMRPLQVGLVLTLIATSLAGVLS
NPLLTIVNSSVADVPRFKQATIPTSIDVAIQPDTPTIAPEL

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=391, Percent_Identity=25.8312020460358, Blast_Score=87, Evalue=6e-17,
Organism=Escherichia coli, GI145693160, Length=426, Percent_Identity=33.0985915492958, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1788831, Length=352, Percent_Identity=25, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788827, Length=331, Percent_Identity=26.2839879154079, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788613, Length=343, Percent_Identity=25.6559766763848, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788829, Length=229, Percent_Identity=27.0742358078603, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788614, Length=327, Percent_Identity=24.1590214067278, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NU2C_ACAM1 (B0BYP3)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516373.1
- GeneID:   5680858
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2044
- HOGENOM:   HBG747830
- OMA:   ILMTITI
- ProtClustDB:   PRK02504
- BioCyc:   AMAR329726:AM1_2044-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: 1.6.5.3

Molecular weight: Translated: 55942; Mature: 55942

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xf496960)-; HASH(0xf4e6990)-; HASH(0xdf05dbc)-; HASH(0xf35ac40)-; HASH(0xf4d873c)-; HASH(0xe76da40)-; HASH(0xf4dd984)-; HASH(0xf4baf84)-; HASH(0xe76adf4)-; HASH(0xe9a8d08)-; HASH(0xf4dd78c)-; HASH(0xf4d870c)-; HASH(0xecadb38)-; HASH(0xeeada70)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVANLASQLNATTILPEGVLVISLILVLLGDITFGRSSAKWTPYLAIASLAACLYLLYT
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QWDQANPIGFLGSFNADNLSIVFRAIIALSTLVTILMSVRYIEQSGSSIGEFMTVLLTAT
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VGAMFLCGAEELVMIFISLETLSIASYLMTGYMKRDSRSNEAALKYLLIGAASSAVFLYG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHH
LSLLYGLSGGKTHLDDIALALTDTSKSLALVISLVFVIASVSFKIAAVPFHQWTPDVYEG
HHHHHCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
SPTPVVAFLSVGSKAAGFALAIRLLTQAFPNLVEQWQFIFTALAILSMVLGNVVAIAQTS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKRMLAYSSIGQAGFVMIGLVIGTEAGYASMVFYLLIYLFMNLGAFTCVILFSLRTGTDE
HHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IGDYAGLYLKDPLLTLALSLCLLSLGGIPPLAGFFGKLYLFWAGWQAGAYGLVLLGLITS
HHCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
VVSIYYYIRVIKMMVVKEPQDMSEVIKNYPSIVWKPLGMRPLQVGLVLTLIATSLAGVLS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHC
NPLLTIVNSSVADVPRFKQATIPTSIDVAIQPDTPTIAPEL
CCHHHHHCCCHHCCCCCCCCCCCCEEEEEECCCCCCCCCCH
>Mature Secondary Structure
MDVANLASQLNATTILPEGVLVISLILVLLGDITFGRSSAKWTPYLAIASLAACLYLLYT
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QWDQANPIGFLGSFNADNLSIVFRAIIALSTLVTILMSVRYIEQSGSSIGEFMTVLLTAT
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VGAMFLCGAEELVMIFISLETLSIASYLMTGYMKRDSRSNEAALKYLLIGAASSAVFLYG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHH
LSLLYGLSGGKTHLDDIALALTDTSKSLALVISLVFVIASVSFKIAAVPFHQWTPDVYEG
HHHHHCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
SPTPVVAFLSVGSKAAGFALAIRLLTQAFPNLVEQWQFIFTALAILSMVLGNVVAIAQTS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKRMLAYSSIGQAGFVMIGLVIGTEAGYASMVFYLLIYLFMNLGAFTCVILFSLRTGTDE
HHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IGDYAGLYLKDPLLTLALSLCLLSLGGIPPLAGFFGKLYLFWAGWQAGAYGLVLLGLITS
HHCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
VVSIYYYIRVIKMMVVKEPQDMSEVIKNYPSIVWKPLGMRPLQVGLVLTLIATSLAGVLS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHC
NPLLTIVNSSVADVPRFKQATIPTSIDVAIQPDTPTIAPEL
CCHHHHHCCCHHCCCCCCCCCCCCEEEEEECCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA