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The map label for this gene is gatA [H]

Identifier: 158335190

GI number: 158335190

Start: 2022126

End: 2023499

Strand: Reverse

Name: gatA [H]

Synonym: AM1_2033

Alternate gene names: 158335190

Gene position: 2023499-2022126 (Counterclockwise)

Preceding gene: 158335191

Following gene: 158335189

Centisome position: 31.11

GC content: 52.98

Gene sequence:

>1374_bases
ATGAGTTTGACGGCAGCTCAAATTACTCACCAGGTTAAAACTGGAGAGCGTTCAGCCACATCCATCGTTGAAGCAGCATT
GACGCATATCTCTAGTCGAGAGGATCACAACTGCTTTACGACACTTCTAGAGTCTTCTGCCTTAGCCAATGCCGAAACCC
TGGATCGCAAACTGGCTGCGGGAGAAACTACAGGTCCTTTAGCAGGAGTCCCATTTGCGGTTAAAAACCTGTTTGATATT
GCTGGAACCGTAACCTTAGCCGGATCCAAAATTAATCAAAGCCAGCCCCCTGCAACTCAAGATGCAACTGCGGTAGCCCG
CCTCAAAGCTGCAGGGGCAATTTTGGTGGGAGCGCTGAATATGGATGAATATGCCTATGGCTTTGTCACCATCAATCATC
ACTATGGCGTTACCCCCAATCCCCATGATCCAACTCGAATGTCCGGTGGCTCCTCGGGTGGATCGGCGGCAGCAGTGGCG
GTTGGCCTCGTACCCCTGACCCTAGGCTCGGATACCAATGGTTCCATTCGGGTGCCAGCCTCGTTGTGCGGCGTTTATGG
TTTCAAGCCCACCTATGGCCGTCTGTCGCGAGCGGGAGTCTACCTGTTTTCCAGCAGTTTGGATCATATTGGTCCCTTTG
CCCGCTCTGTGGAAGATATTGCCCTGGCTTTTGAGGTGCTTCAAGGATCGGACCCTCGCGACCCCGTTTGTACAGACCGT
CCGCCTGTTGCCCCTGATAAATCAGGGGATATGACGGGAATACGGGTTGCGATAGCAACTGATCATTTCGCCCGAGGCAT
GACCTCTACCGTAGCCCAGGCCCTAGACAAAGTCTCGTCGGCATTACAGGTCACCCAAACCGTGACCTTACCTGAATCGG
CACGATCTAGAGCTGCCGCTTATCTGATTACCGCCAGTGAAGGTAGTCAGCTCCACTTACAGCGTTTACAAACGCGTCTA
CAGGACTTTGACCCAGCCACCCGCGATCGATTTGTGGCAGGAGCAATGATGCCGAATAGCTGGTACCTTCAAGCCCAGCG
CTTTCGCCGTTGGTATCGCGATCAGGTCCGCGAAATTTTCAAGACCGTCGATCTGATAATTGCCCCCACAACGCCTTGTC
CAGCCCCATTAATTGATCAGACGACGATGGAGCTAGATGGACAAACGGTGCCCATTCGTCCCCATCTAGGCTTTTACACC
CAACCTCTCTCCTTTATTGGCTTACCCGTGCTCTCAGTGCCCATCTTCCTACCAGGAGAACTGCCCGTTGGCATCCAGCT
GATCGCTCGCCCCTATGACGAGAACACGATTTTTAAAGCGGCTGTGACCCTTGAATCACAAGGGATTACAGAAGCCAAGA
TTGCCCATGCCTAG

Upstream 100 bases:

>100_bases
CAGGCGTGGTCGAGAGCTTTGAGCAGATCCACAGGATTGCTCAGCCCGTTTTAGACTTTCCTTTGCCGGATACCTTAGAG
GCTGCGCCTCAGTTTGAACC

Downstream 100 bases:

>100_bases
AGACAGCCTTAATATCCACTACGCTGGAAGCAACGTCAAGATTCCCTTTGGCACTTAACTATTCCGCCAAGTTCTTCGAG
GTATCCTGTATGGCTACGCT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSLTAAQITHQVKTGERSATSIVEAALTHISSREDHNCFTTLLESSALANAETLDRKLAAGETTGPLAGVPFAVKNLFDI
AGTVTLAGSKINQSQPPATQDATAVARLKAAGAILVGALNMDEYAYGFVTINHHYGVTPNPHDPTRMSGGSSGGSAAAVA
VGLVPLTLGSDTNGSIRVPASLCGVYGFKPTYGRLSRAGVYLFSSSLDHIGPFARSVEDIALAFEVLQGSDPRDPVCTDR
PPVAPDKSGDMTGIRVAIATDHFARGMTSTVAQALDKVSSALQVTQTVTLPESARSRAAAYLITASEGSQLHLQRLQTRL
QDFDPATRDRFVAGAMMPNSWYLQAQRFRRWYRDQVREIFKTVDLIIAPTTPCPAPLIDQTTMELDGQTVPIRPHLGFYT
QPLSFIGLPVLSVPIFLPGELPVGIQLIARPYDENTIFKAAVTLESQGITEAKIAHA

Sequences:

>Translated_457_residues
MSLTAAQITHQVKTGERSATSIVEAALTHISSREDHNCFTTLLESSALANAETLDRKLAAGETTGPLAGVPFAVKNLFDI
AGTVTLAGSKINQSQPPATQDATAVARLKAAGAILVGALNMDEYAYGFVTINHHYGVTPNPHDPTRMSGGSSGGSAAAVA
VGLVPLTLGSDTNGSIRVPASLCGVYGFKPTYGRLSRAGVYLFSSSLDHIGPFARSVEDIALAFEVLQGSDPRDPVCTDR
PPVAPDKSGDMTGIRVAIATDHFARGMTSTVAQALDKVSSALQVTQTVTLPESARSRAAAYLITASEGSQLHLQRLQTRL
QDFDPATRDRFVAGAMMPNSWYLQAQRFRRWYRDQVREIFKTVDLIIAPTTPCPAPLIDQTTMELDGQTVPIRPHLGFYT
QPLSFIGLPVLSVPIFLPGELPVGIQLIARPYDENTIFKAAVTLESQGITEAKIAHA
>Mature_456_residues
SLTAAQITHQVKTGERSATSIVEAALTHISSREDHNCFTTLLESSALANAETLDRKLAAGETTGPLAGVPFAVKNLFDIA
GTVTLAGSKINQSQPPATQDATAVARLKAAGAILVGALNMDEYAYGFVTINHHYGVTPNPHDPTRMSGGSSGGSAAAVAV
GLVPLTLGSDTNGSIRVPASLCGVYGFKPTYGRLSRAGVYLFSSSLDHIGPFARSVEDIALAFEVLQGSDPRDPVCTDRP
PVAPDKSGDMTGIRVAIATDHFARGMTSTVAQALDKVSSALQVTQTVTLPESARSRAAAYLITASEGSQLHLQRLQTRLQ
DFDPATRDRFVAGAMMPNSWYLQAQRFRRWYRDQVREIFKTVDLIIAPTTPCPAPLIDQTTMELDGQTVPIRPHLGFYTQ
PLSFIGLPVLSVPIFLPGELPVGIQLIARPYDENTIFKAAVTLESQGITEAKIAHA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=495, Percent_Identity=28.2828282828283, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI195972892, Length=239, Percent_Identity=30.9623430962343, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI166795287, Length=186, Percent_Identity=37.6344086021505, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=459, Percent_Identity=28.322440087146, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=25.6, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=190, Percent_Identity=36.3157894736842, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=257, Percent_Identity=29.1828793774319, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=239, Percent_Identity=31.7991631799163, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=253, Percent_Identity=29.2490118577075, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=253, Percent_Identity=29.2490118577075, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=418, Percent_Identity=29.6650717703349, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6323950, Length=387, Percent_Identity=27.390180878553, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=236, Percent_Identity=28.8135593220339, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648113, Length=493, Percent_Identity=29.6146044624746, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI21356731, Length=235, Percent_Identity=33.1914893617021, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24644968, Length=498, Percent_Identity=25.9036144578313, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45550774, Length=489, Percent_Identity=23.9263803680982, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648435, Length=489, Percent_Identity=23.9263803680982, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648437, Length=489, Percent_Identity=23.9263803680982, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161078093, Length=496, Percent_Identity=25, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652985, Length=484, Percent_Identity=26.0330578512397, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922090, Length=484, Percent_Identity=26.0330578512397, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652981, Length=484, Percent_Identity=26.0330578512397, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652983, Length=484, Percent_Identity=26.0330578512397, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648441, Length=418, Percent_Identity=25.1196172248804, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24648439, Length=418, Percent_Identity=25.1196172248804, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48709; Mature: 48578

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTAAQITHQVKTGERSATSIVEAALTHISSREDHNCFTTLLESSALANAETLDRKLAA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHC
GETTGPLAGVPFAVKNLFDIAGTVTLAGSKINQSQPPATQDATAVARLKAAGAILVGALN
CCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEEC
MDEYAYGFVTINHHYGVTPNPHDPTRMSGGSSGGSAAAVAVGLVPLTLGSDTNGSIRVPA
CCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEEEEEECCCCCCCEECCH
SLCGVYGFKPTYGRLSRAGVYLFSSSLDHIGPFARSVEDIALAFEVLQGSDPRDPVCTDR
HHHHHCCCCCCHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PPVAPDKSGDMTGIRVAIATDHFARGMTSTVAQALDKVSSALQVTQTVTLPESARSRAAA
CCCCCCCCCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHEEECCHHHHCCEEE
YLITASEGSQLHLQRLQTRLQDFDPATRDRFVAGAMMPNSWYLQAQRFRRWYRDQVREIF
EEEECCCCCHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
KTVDLIIAPTTPCPAPLIDQTTMELDGQTVPIRPHLGFYTQPLSFIGLPVLSVPIFLPGE
HHHHEEEECCCCCCCCCCCCCHHCCCCCEEEECCCCCCCCCCHHHHCCHHHHCCEEECCC
LPVGIQLIARPYDENTIFKAAVTLESQGITEAKIAHA
CCCCEEEEEECCCCCCEEEEEEEEECCCCCHHHHCCC
>Mature Secondary Structure 
SLTAAQITHQVKTGERSATSIVEAALTHISSREDHNCFTTLLESSALANAETLDRKLAA
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHC
GETTGPLAGVPFAVKNLFDIAGTVTLAGSKINQSQPPATQDATAVARLKAAGAILVGALN
CCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEEC
MDEYAYGFVTINHHYGVTPNPHDPTRMSGGSSGGSAAAVAVGLVPLTLGSDTNGSIRVPA
CCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEEEEEECCCCCCCEECCH
SLCGVYGFKPTYGRLSRAGVYLFSSSLDHIGPFARSVEDIALAFEVLQGSDPRDPVCTDR
HHHHHCCCCCCHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PPVAPDKSGDMTGIRVAIATDHFARGMTSTVAQALDKVSSALQVTQTVTLPESARSRAAA
CCCCCCCCCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHEEECCHHHHCCEEE
YLITASEGSQLHLQRLQTRLQDFDPATRDRFVAGAMMPNSWYLQAQRFRRWYRDQVREIF
EEEECCCCCHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
KTVDLIIAPTTPCPAPLIDQTTMELDGQTVPIRPHLGFYTQPLSFIGLPVLSVPIFLPGE
HHHHEEEECCCCCCCCCCCCCHHCCCCCEEEECCCCCCCCCCHHHHCCHHHHCCEEECCC
LPVGIQLIARPYDENTIFKAAVTLESQGITEAKIAHA
CCCCEEEEEECCCCCCEEEEEEEEECCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA