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The map label for this gene is thiE [H]

Identifier: 158335116

GI number: 158335116

Start: 1952037

End: 1953074

Strand: Direct

Name: thiE [H]

Synonym: AM1_1957

Alternate gene names: 158335116

Gene position: 1952037-1953074 (Clockwise)

Preceding gene: 158335115

Following gene: 158335117

Centisome position: 30.01

GC content: 52.89

Gene sequence:

>1038_bases
ATGATGAATGCAAACTCGACCCTTTATCGCATCTTGGATGCCAACCTCGATCGAGCACGGGAAGGGTTGCGGGTGATTGA
AGAATGGTGTCGATTTGGTCTAGAAGATTCTCAGATGAGTGCTCAGTGCAAGCAACTCCGCCAGGAATTGGCCCATTGGC
ATCATCCCCCCTTACGGATGGCCCGAGATACCCCCAATGATCCAGGCACCCAGCTCACCCATCCCCAAGAGGCAATCCGT
ACTGACTTGCCGACGGTTGTACAAGCCAATCTTTGCCGGGTACAAGAAGCCCTGCGAGTCCTAGAGGAATACAGCAAGAT
TTACGATACCCAGATGGCAGCGGCCTGTAAGCAGATGCGATATCAGGTGTATACCCTAGACACTCAACTCCAGCCTCGCC
ATCGCTTGCAGCAATTACTTGATGCGCCGCTCTATCTAGTCACGTCCCCCAGCGACCAGTTGACCGCCGTCGTCGAAGCC
GCCTTAAAGGGAGGACTAAAGCTGGTTCAATATCGAGAGAAACAAGCGGACGACGATCAGCGCTTGGCCGTTGCCCAGAC
TTTATGTCGGCTATGCCATCAATATAATGCTCTGTTTATCGTCAATGATCGAATTGATATTGCTGTAGCCGTGGATGCCG
ATGGGGTTCACTTAGGACAGCAAGACATGACTATGGCGGTGGCCCGTCAAATGTTAGGCCCCGGTAAGCTAGTAGGGCGC
TCCACCACCAATCCGCAAGAAATGGAAAGAGCCATCCAAGAAGAAGCAGACTATATTGGCGTCGGCCCCATCTATGAAAC
CCCTACCAAAGCAGGCAAAGCTGCCGTTGGTCATGAGTACATTCGCTATGCCAATCAACATGCACCCATGCCCTATTACG
CCATCGGCGGCATTAACGAGTCCAATCTTGGCGAAGTCTTGGCCGCAGGCGCCCAACGGGTAGCTGTGGTGAGAGCGATT
ATGCATGCCCCTTCCCCAACCCAGGCTGTCCAACAGATGTTAAAGCAGTTTCCACAAACATCTGCTCCAGCCCCATAA

Upstream 100 bases:

>100_bases
AAATAATAAGGATATTAACACTCTTCTACCTCAGTTGAGTGCTTGTTTAGACTCAACCACTTGATAGGATAGAAAAGGTT
TATTCGTAATACTTTTTTTA

Downstream 100 bases:

>100_bases
AGTCGGTCTAGAAGAGAGACCACATTGGCGCATTCTATACCTCCAGCTCATCCACCAAGCTTCAGGATTTATTTTTGATC
GTGAACCTAATATGGAAAGA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MMNANSTLYRILDANLDRAREGLRVIEEWCRFGLEDSQMSAQCKQLRQELAHWHHPPLRMARDTPNDPGTQLTHPQEAIR
TDLPTVVQANLCRVQEALRVLEEYSKIYDTQMAAACKQMRYQVYTLDTQLQPRHRLQQLLDAPLYLVTSPSDQLTAVVEA
ALKGGLKLVQYREKQADDDQRLAVAQTLCRLCHQYNALFIVNDRIDIAVAVDADGVHLGQQDMTMAVARQMLGPGKLVGR
STTNPQEMERAIQEEADYIGVGPIYETPTKAGKAAVGHEYIRYANQHAPMPYYAIGGINESNLGEVLAAGAQRVAVVRAI
MHAPSPTQAVQQMLKQFPQTSAPAP

Sequences:

>Translated_345_residues
MMNANSTLYRILDANLDRAREGLRVIEEWCRFGLEDSQMSAQCKQLRQELAHWHHPPLRMARDTPNDPGTQLTHPQEAIR
TDLPTVVQANLCRVQEALRVLEEYSKIYDTQMAAACKQMRYQVYTLDTQLQPRHRLQQLLDAPLYLVTSPSDQLTAVVEA
ALKGGLKLVQYREKQADDDQRLAVAQTLCRLCHQYNALFIVNDRIDIAVAVDADGVHLGQQDMTMAVARQMLGPGKLVGR
STTNPQEMERAIQEEADYIGVGPIYETPTKAGKAAVGHEYIRYANQHAPMPYYAIGGINESNLGEVLAAGAQRVAVVRAI
MHAPSPTQAVQQMLKQFPQTSAPAP
>Mature_345_residues
MMNANSTLYRILDANLDRAREGLRVIEEWCRFGLEDSQMSAQCKQLRQELAHWHHPPLRMARDTPNDPGTQLTHPQEAIR
TDLPTVVQANLCRVQEALRVLEEYSKIYDTQMAAACKQMRYQVYTLDTQLQPRHRLQQLLDAPLYLVTSPSDQLTAVVEA
ALKGGLKLVQYREKQADDDQRLAVAQTLCRLCHQYNALFIVNDRIDIAVAVDADGVHLGQQDMTMAVARQMLGPGKLVGR
STTNPQEMERAIQEEADYIGVGPIYETPTKAGKAAVGHEYIRYANQHAPMPYYAIGGINESNLGEVLAAGAQRVAVVRAI
MHAPSPTQAVQQMLKQFPQTSAPAP

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=180, Percent_Identity=32.2222222222222, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=218, Percent_Identity=36.697247706422, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 38524; Mature: 38524

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNANSTLYRILDANLDRAREGLRVIEEWCRFGLEDSQMSAQCKQLRQELAHWHHPPLRM
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
ARDTPNDPGTQLTHPQEAIRTDLPTVVQANLCRVQEALRVLEEYSKIYDTQMAAACKQMR
CCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQVYTLDTQLQPRHRLQQLLDAPLYLVTSPSDQLTAVVEAALKGGLKLVQYREKQADDDQ
HEEEEECCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHH
RLAVAQTLCRLCHQYNALFIVNDRIDIAVAVDADGVHLGQQDMTMAVARQMLGPGKLVGR
HHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCC
STTNPQEMERAIQEEADYIGVGPIYETPTKAGKAAVGHEYIRYANQHAPMPYYAIGGINE
CCCCHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCC
SNLGEVLAAGAQRVAVVRAIMHAPSPTQAVQQMLKQFPQTSAPAP
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMNANSTLYRILDANLDRAREGLRVIEEWCRFGLEDSQMSAQCKQLRQELAHWHHPPLRM
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
ARDTPNDPGTQLTHPQEAIRTDLPTVVQANLCRVQEALRVLEEYSKIYDTQMAAACKQMR
CCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQVYTLDTQLQPRHRLQQLLDAPLYLVTSPSDQLTAVVEAALKGGLKLVQYREKQADDDQ
HEEEEECCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHH
RLAVAQTLCRLCHQYNALFIVNDRIDIAVAVDADGVHLGQQDMTMAVARQMLGPGKLVGR
HHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCC
STTNPQEMERAIQEEADYIGVGPIYETPTKAGKAAVGHEYIRYANQHAPMPYYAIGGINE
CCCCHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCC
SNLGEVLAAGAQRVAVVRAIMHAPSPTQAVQQMLKQFPQTSAPAP
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA