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The map label for this gene is glyA

Identifier: 158335097

GI number: 158335097

Start: 1932053

End: 1933333

Strand: Direct

Name: glyA

Synonym: AM1_1938

Alternate gene names: 158335097

Gene position: 1932053-1933333 (Clockwise)

Preceding gene: 158335091

Following gene: 158335098

Centisome position: 29.71

GC content: 50.51

Gene sequence:

>1281_bases
ATGCAAACTAGCTTAGATATTTTGACAGAAACCGATCCTGCGATCGCAGGGATACTACAGCAGGAATTACAACGCCAGCG
CGATCATTTAGAGCTGATTGCCAGCGAGAACTTCACCTCAGCAGCAGTCTTGGCCGCGCAAGGGTCGGTCCTAACCAATA
AGTATGCAGAAGGGCTACCTGGCAAGCGGTATTACGGTGGCTGTGAATATATCGATGCGGCCGAACAGCTCGCTATTGAT
CGAGCCAAAGAACTATTTGGTGCAGCCCATGTAAATGTCCAGCCTCACTCTGGTGCTCAGGCAAACTTTGCCGTCTTTCT
CACCTTGCTGCAGCCAGGCGATACCTTTATGGGCATGGATTTGTCCCATGGGGGTCACTTAACCCATGGCTCTCCAGTGA
ATGTCTCAGGGAAATGGTTCAATGTCGTCCAGTACGGTGTCGATCCAAATTCAGAACAACTCAACTACGATACGATTCGC
GAGCTAGCCCTTAAGCATCGTCCCAAAATGATTGTCTGTGGATACTCTGCCTATCCTCGCATCATTGATTTTGAGAAGTT
TCGGGCCATCGCTGATGAGATCGATGCCTATTTAATGGCAGACATTGCCCATATTGCTGGCTTAGTCGCCTCAGGGCATC
ATCCCAATCCACTCCCCTTCTGCGACGTGGTCACCACCACCACGCACAAAACCCTCCGAGGTCCCCGAGGCGGCCTGATC
ATGACCAAGGATCTTGAGCTGGGTAAAAAGTTTGATAAATCCGTTTTCCCGGGTACCCAAGGCGGACCGCTAGAACATGT
CATTGCGGCTAAAGCCGTGGCCTTCGGCGAAGCCCTCAAGCCTGATTTCAGAGATTATTGTGGTCATGTGGTTGAGAATG
CCCAAACCTTAGCCCAACAACTGCAAGAGCGCGGCTTCAAAATCGTTTCTAACGGTACGGATAATCACCTCTTACTGGTT
GATTTACGATCTATTGGTATGACCGGAAAGCAGGCCGACCAGCGAGTCAGTCAAGTCAATATCACAGCCAACAAAAACAC
CGTTCCCTTCGATCCTGAATCTCCATTTGTCACGAGTGGACTCCGGTTAGGATCTCCTGCCATGACGACTCGGGGCATGG
GAACAGCAGAATTTACCGAGATAGCCAATATCATTGCGGATTGCCTGCTAAAACCTGAAGATGCTGCTGTAACAGAAGAC
TGCCGCCAGCGGGTTGCCAATCTTTGTTCACGATTCCCCCTCTATCCTCATCTCACCAGTCCTGTGCCCGCTTTAACTTA
G

Upstream 100 bases:

>100_bases
CGTTGGGAAAGCAAGAAAAATACGCAGCACAAGACCTATTCAATGGATTGATGCTGTTCGATCGTAGCTTTGTTGACACT
GGAAAAGGATAGGCTGTGCC

Downstream 100 bases:

>100_bases
CCAGGATCTCCCTTGGTTTGGCAACGTAGCTTGTGTTAATCCCCATTCCTCATGCATGATGTCATTGTGACAGTTCATTA
AGACTGTTTTCTATTCTGCA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MQTSLDILTETDPAIAGILQQELQRQRDHLELIASENFTSAAVLAAQGSVLTNKYAEGLPGKRYYGGCEYIDAAEQLAID
RAKELFGAAHVNVQPHSGAQANFAVFLTLLQPGDTFMGMDLSHGGHLTHGSPVNVSGKWFNVVQYGVDPNSEQLNYDTIR
ELALKHRPKMIVCGYSAYPRIIDFEKFRAIADEIDAYLMADIAHIAGLVASGHHPNPLPFCDVVTTTTHKTLRGPRGGLI
MTKDLELGKKFDKSVFPGTQGGPLEHVIAAKAVAFGEALKPDFRDYCGHVVENAQTLAQQLQERGFKIVSNGTDNHLLLV
DLRSIGMTGKQADQRVSQVNITANKNTVPFDPESPFVTSGLRLGSPAMTTRGMGTAEFTEIANIIADCLLKPEDAAVTED
CRQRVANLCSRFPLYPHLTSPVPALT

Sequences:

>Translated_426_residues
MQTSLDILTETDPAIAGILQQELQRQRDHLELIASENFTSAAVLAAQGSVLTNKYAEGLPGKRYYGGCEYIDAAEQLAID
RAKELFGAAHVNVQPHSGAQANFAVFLTLLQPGDTFMGMDLSHGGHLTHGSPVNVSGKWFNVVQYGVDPNSEQLNYDTIR
ELALKHRPKMIVCGYSAYPRIIDFEKFRAIADEIDAYLMADIAHIAGLVASGHHPNPLPFCDVVTTTTHKTLRGPRGGLI
MTKDLELGKKFDKSVFPGTQGGPLEHVIAAKAVAFGEALKPDFRDYCGHVVENAQTLAQQLQERGFKIVSNGTDNHLLLV
DLRSIGMTGKQADQRVSQVNITANKNTVPFDPESPFVTSGLRLGSPAMTTRGMGTAEFTEIANIIADCLLKPEDAAVTED
CRQRVANLCSRFPLYPHLTSPVPALT
>Mature_426_residues
MQTSLDILTETDPAIAGILQQELQRQRDHLELIASENFTSAAVLAAQGSVLTNKYAEGLPGKRYYGGCEYIDAAEQLAID
RAKELFGAAHVNVQPHSGAQANFAVFLTLLQPGDTFMGMDLSHGGHLTHGSPVNVSGKWFNVVQYGVDPNSEQLNYDTIR
ELALKHRPKMIVCGYSAYPRIIDFEKFRAIADEIDAYLMADIAHIAGLVASGHHPNPLPFCDVVTTTTHKTLRGPRGGLI
MTKDLELGKKFDKSVFPGTQGGPLEHVIAAKAVAFGEALKPDFRDYCGHVVENAQTLAQQLQERGFKIVSNGTDNHLLLV
DLRSIGMTGKQADQRVSQVNITANKNTVPFDPESPFVTSGLRLGSPAMTTRGMGTAEFTEIANIIADCLLKPEDAAVTED
CRQRVANLCSRFPLYPHLTSPVPALT

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:
GO:0003824: Serine hydroxymethyltransferase
GO:0004372: Serine hydroxymethyltransferase
GO:0005737: Serine hydroxymethyltransferase
GO:0006544: Serine hydroxymethyltransferase
GO:0006563: Serine hydroxymethyltransferase
GO:0006730: Serine hydroxymethyltransferase
GO:0016740: Serine hydroxymethyltransferase
GO:0030170: Serine hydroxymethyltransferase

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=456, Percent_Identity=43.2017543859649, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI261862350, Length=456, Percent_Identity=43.2017543859649, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI261862348, Length=456, Percent_Identity=43.2017543859649, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI19923315, Length=456, Percent_Identity=43.2017543859649, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI261862346, Length=451, Percent_Identity=43.6807095343681, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=46.6334164588529, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI22547189, Length=387, Percent_Identity=45.2196382428941, Blast_Score=298, Evalue=8e-81,
Organism=Escherichia coli, GI1788902, Length=410, Percent_Identity=57.8048780487805, Blast_Score=480, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI25144732, Length=403, Percent_Identity=46.1538461538462, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI25144729, Length=403, Percent_Identity=46.1538461538462, Blast_Score=354, Evalue=5e-98,
Organism=Saccharomyces cerevisiae, GI6319739, Length=434, Percent_Identity=45.1612903225806, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6323087, Length=431, Percent_Identity=42.2273781902552, Blast_Score=342, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24640005, Length=453, Percent_Identity=42.3841059602649, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=453, Percent_Identity=42.3841059602649, Blast_Score=361, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_ACAM1 (B0CEI9)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516269.1
- ProteinModelPortal:   B0CEI9
- SMR:   B0CEI9
- GeneID:   5680753
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1938
- HOGENOM:   HBG301263
- OMA:   GGLIMTK
- ProtClustDB:   PRK00011
- BioCyc:   AMAR329726:AM1_1938-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46307; Mature: 46307

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSLDILTETDPAIAGILQQELQRQRDHLELIASENFTSAAVLAAQGSVLTNKYAEGLP
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCHHHHHHHCCCC
GKRYYGGCEYIDAAEQLAIDRAKELFGAAHVNVQPHSGAQANFAVFLTLLQPGDTFMGMD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEEECCCCCEEEEE
LSHGGHLTHGSPVNVSGKWFNVVQYGVDPNSEQLNYDTIRELALKHRPKMIVCGYSAYPR
ECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCC
IIDFEKFRAIADEIDAYLMADIAHIAGLVASGHHPNPLPFCDVVTTTTHKTLRGPRGGLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEECCCHHHHCCCCCCEE
MTKDLELGKKFDKSVFPGTQGGPLEHVIAAKAVAFGEALKPDFRDYCGHVVENAQTLAQQ
EECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LQERGFKIVSNGTDNHLLLVDLRSIGMTGKQADQRVSQVNITANKNTVPFDPESPFVTSG
HHHCCCEEEECCCCCEEEEEEHHHCCCCCHHHHHHHHEEEEEECCCCCCCCCCCCCHHCC
LRLGSPAMTTRGMGTAEFTEIANIIADCLLKPEDAAVTEDCRQRVANLCSRFPLYPHLTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
PVPALT
CCCCCC
>Mature Secondary Structure
MQTSLDILTETDPAIAGILQQELQRQRDHLELIASENFTSAAVLAAQGSVLTNKYAEGLP
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCHHHHHHHCCCC
GKRYYGGCEYIDAAEQLAIDRAKELFGAAHVNVQPHSGAQANFAVFLTLLQPGDTFMGMD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEEECCCCCEEEEE
LSHGGHLTHGSPVNVSGKWFNVVQYGVDPNSEQLNYDTIRELALKHRPKMIVCGYSAYPR
ECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCC
IIDFEKFRAIADEIDAYLMADIAHIAGLVASGHHPNPLPFCDVVTTTTHKTLRGPRGGLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEECCCHHHHCCCCCCEE
MTKDLELGKKFDKSVFPGTQGGPLEHVIAAKAVAFGEALKPDFRDYCGHVVENAQTLAQQ
EECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LQERGFKIVSNGTDNHLLLVDLRSIGMTGKQADQRVSQVNITANKNTVPFDPESPFVTSG
HHHCCCEEEECCCCCEEEEEEHHHCCCCCHHHHHHHHEEEEEECCCCCCCCCCCCCHHCC
LRLGSPAMTTRGMGTAEFTEIANIIADCLLKPEDAAVTEDCRQRVANLCSRFPLYPHLTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
PVPALT
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA