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The map label for this gene is ycgF [H]

Identifier: 158335093

GI number: 158335093

Start: 1929311

End: 1930075

Strand: Reverse

Name: ycgF [H]

Synonym: AM1_1934

Alternate gene names: 158335093

Gene position: 1930075-1929311 (Counterclockwise)

Preceding gene: 158335094

Following gene: 158335092

Centisome position: 29.68

GC content: 44.84

Gene sequence:

>765_bases
GTGGTGGATCAAGAAACTCTGTCAGTGGGATGTTCTGGGTGTCTGAGTACGCCTGAACTGCAGTTTGACTTCACAATGGC
GTTTCAGCCCATTGTGAACCTGGGGAACGGCACTATTTTTGCGCAAGAAGCCCTAGTTCGTGGTCTTAATAATGAGTCGG
CGGCGAGCATTCTTAGCCAGGTGACCGATCAAAGTCGATATCGTTTTGACCAAGCCTGCCGAGTTCGGGCGATTCAGCTA
GCGGCTGAGCTGGGAATCCAGTCAATGGTCAGTATCAATTTTCTCCCGAATGCCGTTTATCAGCCCGATCTTTGTATTCG
TACTACGATCGAAACGGCTAAACAATGCAATTTTCCGTTGAACAAAATTCTGTTTGAGTTAACGGAAGTTGAGAAGGTGA
AGGACTATGCTCATGTCCGCGCCATCATTGAGCATTACCAAAAATTAGGATTTAAAACGGCATTAGATGACTTTGGAGCT
GGCTATTCTGGCTTGAACTTTTTAGCTGATTTTCAGCCGGATTTTATTAAGCTGGATATGGCGTTAATCCGTAATATTGA
CCAAGATAAAGGTCGGCAAGCCATTGTCAGAGGGATTATTCAAGTCTGTCGTGAGCTAGCGATTGAGCCTATTGCAGAGG
GTATAGAGACCGAAGCGGAATTGGCTGTTCTCAAGCAAATGGATATTCAGCTATTTCAAGGCTATTATTTTGCCAAACCT
AGTTTCAGGTCGATTACCACTCATTTAAATCTGTCTCCTGGTTAG

Upstream 100 bases:

>100_bases
AGATCTTGATTCCATGTATTTGGATGAGATGGATTTCTACTTACATCAATAGCCATGTGCGGTAGCTTCGGTTTTTTATC
TGTGATTTCAAAACACTATC

Downstream 100 bases:

>100_bases
GGGTTGCCTGAAAAATTCCTTGGTGCCAGATAGGTAAAAGATGGTTTTTGCCAGGGCATTGAATGTTAGAATTCTAGATC
CTTGCTTCTTAAAGAGTTGG

Product: EAL domain-containing protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MVDQETLSVGCSGCLSTPELQFDFTMAFQPIVNLGNGTIFAQEALVRGLNNESAASILSQVTDQSRYRFDQACRVRAIQL
AAELGIQSMVSINFLPNAVYQPDLCIRTTIETAKQCNFPLNKILFELTEVEKVKDYAHVRAIIEHYQKLGFKTALDDFGA
GYSGLNFLADFQPDFIKLDMALIRNIDQDKGRQAIVRGIIQVCRELAIEPIAEGIETEAELAVLKQMDIQLFQGYYFAKP
SFRSITTHLNLSPG

Sequences:

>Translated_254_residues
MVDQETLSVGCSGCLSTPELQFDFTMAFQPIVNLGNGTIFAQEALVRGLNNESAASILSQVTDQSRYRFDQACRVRAIQL
AAELGIQSMVSINFLPNAVYQPDLCIRTTIETAKQCNFPLNKILFELTEVEKVKDYAHVRAIIEHYQKLGFKTALDDFGA
GYSGLNFLADFQPDFIKLDMALIRNIDQDKGRQAIVRGIIQVCRELAIEPIAEGIETEAELAVLKQMDIQLFQGYYFAKP
SFRSITTHLNLSPG
>Mature_254_residues
MVDQETLSVGCSGCLSTPELQFDFTMAFQPIVNLGNGTIFAQEALVRGLNNESAASILSQVTDQSRYRFDQACRVRAIQL
AAELGIQSMVSINFLPNAVYQPDLCIRTTIETAKQCNFPLNKILFELTEVEKVKDYAHVRAIIEHYQKLGFKTALDDFGA
GYSGLNFLADFQPDFIKLDMALIRNIDQDKGRQAIVRGIIQVCRELAIEPIAEGIETEAELAVLKQMDIQLFQGYYFAKP
SFRSITTHLNLSPG

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=238, Percent_Identity=34.0336134453782, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1787541, Length=167, Percent_Identity=35.9281437125749, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87081845, Length=226, Percent_Identity=30.5309734513274, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790496, Length=227, Percent_Identity=27.7533039647577, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI226510982, Length=230, Percent_Identity=25.2173913043478, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082096, Length=150, Percent_Identity=29.3333333333333, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081980, Length=234, Percent_Identity=26.4957264957265, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87081743, Length=225, Percent_Identity=28.4444444444444, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786507, Length=119, Percent_Identity=31.0924369747899, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787055, Length=228, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788849, Length=150, Percent_Identity=29.3333333333333, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788502, Length=229, Percent_Identity=22.2707423580786, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081921, Length=236, Percent_Identity=22.0338983050847, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 28355; Mature: 28355

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDQETLSVGCSGCLSTPELQFDFTMAFQPIVNLGNGTIFAQEALVRGLNNESAASILSQ
CCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHH
VTDQSRYRFDQACRVRAIQLAAELGIQSMVSINFLPNAVYQPDLCIRTTIETAKQCNFPL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCH
NKILFELTEVEKVKDYAHVRAIIEHYQKLGFKTALDDFGAGYSGLNFLADFQPDFIKLDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCHHHHHHH
ALIRNIDQDKGRQAIVRGIIQVCRELAIEPIAEGIETEAELAVLKQMDIQLFQGYYFAKP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
SFRSITTHLNLSPG
CHHHHEEEEECCCC
>Mature Secondary Structure
MVDQETLSVGCSGCLSTPELQFDFTMAFQPIVNLGNGTIFAQEALVRGLNNESAASILSQ
CCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHH
VTDQSRYRFDQACRVRAIQLAAELGIQSMVSINFLPNAVYQPDLCIRTTIETAKQCNFPL
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCH
NKILFELTEVEKVKDYAHVRAIIEHYQKLGFKTALDDFGAGYSGLNFLADFQPDFIKLDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCHHHHHHH
ALIRNIDQDKGRQAIVRGIIQVCRELAIEPIAEGIETEAELAVLKQMDIQLFQGYYFAKP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
SFRSITTHLNLSPG
CHHHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]