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The map label for this gene is trmB [H]

Identifier: 158335088

GI number: 158335088

Start: 1924565

End: 1925209

Strand: Direct

Name: trmB [H]

Synonym: AM1_1930

Alternate gene names: 158335088

Gene position: 1924565-1925209 (Clockwise)

Preceding gene: 158335083

Following gene: 158335091

Centisome position: 29.59

GC content: 51.47

Gene sequence:

>645_bases
ATGGGTGTTCGAGTTCGTCAGCACGTTAACCCCCTGAGCAAAAAATTCCAACATGCGATCGCACCCCCGGATTGGCCTGA
AATCTTCGCCCAACCAGAGCAGCCCCTTTACTTAGATATTGGCTGTGCCCGTGGGCAGTTTTTACTAGAGATGGCTCAAC
TGCACCCTTACCATAATTTCTTAGGCGTCGAAATTCGGCGGCCCATGGTTGAATCTGCCCTGAAGCGCCGTGATGCCTTA
CAGCTCACCAATCTCCATTTTCTGTTCGGCAACATCAATACGTCCCTCGATTCCCTGGTGGGTGCTCAATCGGTTACGGG
TGTGACGATCCAATTTCCTGACCCTTGGTTTAAGCGTCGCCATCAAAAACGGCGCGTCGTCCAACCCCAGTTAGTGAATG
AACTCGCCATTTGCCTTAAGCCAGAAGGGTTATTGCTGATCCAGTCGGATGTATTGGAGGTGGCGATTGATATGTGCGAT
CGCATCACCGAACATCCTGCCTTTACAGGCACAACGCCGCCCCACCATTGGCTCACAGAAAGCCCGTTTCCCGCTGCAAC
TGAGCGGGAAAAGTTGACCTTATCAGAAGGGTTGCCGGTGTACCGTTATTTATTTCAACGGCGAGTCGAGCCAAAGTCTA
AATAA

Upstream 100 bases:

>100_bases
ACCACGGTGTCGGGGGTTCGAATCCCTCCTGGCCCGTTTTTTAATTAACTCAAGTGTTATCGCTTGGGTGCATTGTCGTT
TCTATCGTTGTCCTCACAGT

Downstream 100 bases:

>100_bases
TCGTGTTATCGGGAATCTCAGCATTTTTCAGCACCACAACAATACCGCTGCGGATGTAAAAGCCATGCTCTTCTTGGTTG
GATTCCTCAACATTGTCTTT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MGVRVRQHVNPLSKKFQHAIAPPDWPEIFAQPEQPLYLDIGCARGQFLLEMAQLHPYHNFLGVEIRRPMVESALKRRDAL
QLTNLHFLFGNINTSLDSLVGAQSVTGVTIQFPDPWFKRRHQKRRVVQPQLVNELAICLKPEGLLLIQSDVLEVAIDMCD
RITEHPAFTGTTPPHHWLTESPFPAATEREKLTLSEGLPVYRYLFQRRVEPKSK

Sequences:

>Translated_214_residues
MGVRVRQHVNPLSKKFQHAIAPPDWPEIFAQPEQPLYLDIGCARGQFLLEMAQLHPYHNFLGVEIRRPMVESALKRRDAL
QLTNLHFLFGNINTSLDSLVGAQSVTGVTIQFPDPWFKRRHQKRRVVQPQLVNELAICLKPEGLLLIQSDVLEVAIDMCD
RITEHPAFTGTTPPHHWLTESPFPAATEREKLTLSEGLPVYRYLFQRRVEPKSK
>Mature_213_residues
GVRVRQHVNPLSKKFQHAIAPPDWPEIFAQPEQPLYLDIGCARGQFLLEMAQLHPYHNFLGVEIRRPMVESALKRRDALQ
LTNLHFLFGNINTSLDSLVGAQSVTGVTIQFPDPWFKRRHQKRRVVQPQLVNELAICLKPEGLLLIQSDVLEVAIDMCDR
ITEHPAFTGTTPPHHWLTESPFPAATEREKLTLSEGLPVYRYLFQRRVEPKSK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:
GO:0006400: tRNA (guanine-N(7)-)-methyltransferase
GO:0008033: tRNA (guanine-N(7)-)-methyltransferase
GO:0008168: tRNA (guanine-N(7)-)-methyltransferase
GO:0008176: tRNA (guanine-N(7)-)-methyltransferase
GO:0016740: tRNA (guanine-N(7)-)-methyltransferase
GO:0032259: tRNA (guanine-N(7)-)-methyltransferase

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI167004078, Length=243, Percent_Identity=29.2181069958848, Blast_Score=85, Evalue=6e-17,
Organism=Escherichia coli, GI1789330, Length=184, Percent_Identity=27.1739130434783, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536689, Length=214, Percent_Identity=26.6355140186916, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320000, Length=185, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20128915, Length=179, Percent_Identity=29.0502793296089, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 24573; Mature: 24442

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVRVRQHVNPLSKKFQHAIAPPDWPEIFAQPEQPLYLDIGCARGQFLLEMAQLHPYHNF
CCCCHHHHCCHHHHHHHHHCCCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCHHH
LGVEIRRPMVESALKRRDALQLTNLHFLFGNINTSLDSLVGAQSVTGVTIQFPDPWFKRR
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCEEEEECCCHHHHHH
HQKRRVVQPQLVNELAICLKPEGLLLIQSDVLEVAIDMCDRITEHPAFTGTTPPHHWLTE
HHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SPFPAATEREKLTLSEGLPVYRYLFQRRVEPKSK
CCCCCCCCCCCEEHHCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GVRVRQHVNPLSKKFQHAIAPPDWPEIFAQPEQPLYLDIGCARGQFLLEMAQLHPYHNF
CCCHHHHCCHHHHHHHHHCCCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCHHH
LGVEIRRPMVESALKRRDALQLTNLHFLFGNINTSLDSLVGAQSVTGVTIQFPDPWFKRR
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCEEEEECCCHHHHHH
HQKRRVVQPQLVNELAICLKPEGLLLIQSDVLEVAIDMCDRITEHPAFTGTTPPHHWLTE
HHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SPFPAATEREKLTLSEGLPVYRYLFQRRVEPKSK
CCCCCCCCCCCEEHHCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]