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The map label for this gene is dapL

Identifier: 158335042

GI number: 158335042

Start: 1874156

End: 1875385

Strand: Direct

Name: dapL

Synonym: AM1_1880

Alternate gene names: 158335042

Gene position: 1874156-1875385 (Clockwise)

Preceding gene: 158335041

Following gene: 158335043

Centisome position: 28.82

GC content: 50.24

Gene sequence:

>1230_bases
ATGGCAACGATTAACGACAATTATTTAAAGCTCAAAGCAGGTTACTTATTTCCTGAGATTGGTCGCCGAGTCAGCGCATT
TGCTGAGGCCAATCCCGACGCACCGATTATCAAGCTTGGTATTGGGGATGTGACCGAACCCCTACCTGAAGCCTGTCGAA
GTGCCATGGTCACCGCCGTTGAAGACATGGGGAATCGCGATTCCTTCAAAGGCTATGGTCCTGAGCAGGGCTATGGCTGG
TTACGGGAAAAAATTGCCGCCCATGATTTTCAGGCCCGAGGTTGTGACGTGGATGCTGGGGAGATTTTTATCTCTGACGG
TTCCAAGTGTGACTGCGGCAATATCCTCGATATTTTTGGCGATAACAACACCATTGCCGTCACCGATCCGGTGTATCCCG
TCTATGTCGATACCAACGTCATGGCCGGACATACGGGTCCTTGCAATGATCAGGGCGAATATGAAGGACTCACCTATTTA
CCGATTAATGCGGGCAATAATTTCACGGCTCAGATTCCCAGCCAAAAGGTGGATTTGATCTATCTCTGTTTCCCCAATAA
CCCCACTGGAGCGGTGGCGAGCAAGAGCCATCTGCAAGATTGGGTGAACTATGCCAAATCCCACGGCTCCATCATTCTGT
TTGATGCCGCCTATGAAGCCTTTATTACCGATCCCGAGATTCCCCACTCCATTTATGAAATTGAGGGGGCCAGAGACTGT
GCGATTGAGTTTCGTTCCTTCTCTAAAAATGCCGGATTTACCGGAACTCGCTGTGCCCTAACGGTTGTCCCCAAAACCTT
AATGGCCAAAGCAGCGGATGAATCCGATGTCGAACTCTGGAAGCTGTGGAACCGTCGCCAATCCACCAAATTTAACGGCG
TCTCCTACATCGTCCAGCGCGGGGCAGAAGCCGTCTATTCTGAAGCGGGTCAGGCCCAAACTAAAGCCCTAATCAGCTTT
TACTTGGAAAATGCCAAAATCATTCGTGAGAAATTAACGGCTGCTGGACTACAAGTGTTTGGTGGCGTTAATGCGCCCTA
TGTGTGGGTGCAGACGCCCAATGGTATTTCTAGCTGGGACTTTTTCGATCAGTTGTTGCATAAAACCAATGTGGTTGGCA
CCCCTGGATCCGGCTTTGGGGCAGCGGGTGAGGGCTATTTTCGGATTTCAGCCTTTAATAGCCGGGCCAATGTGGAAGAA
GCCATGCGGCGGATTACAGCAAACCTCTAA

Upstream 100 bases:

>100_bases
CAGACTGTAAGTTAGGGAATGATGTTGTCAATTTAGCGAAACCGACTCAGTGGAACAGTAACGTTCTAAGCTGGTTGAGA
GTGTTCATCGCTAAGACAAC

Downstream 100 bases:

>100_bases
GGAATTGGGGCTGGACCAATGGCACATGTCGGTAATGTGGTCTCATCGCAATGGCTGCATGAGCATCATGAAGATGCACA
AGTAGTGATTGTGGACTGTC

Product: L,L-diaminopimelate aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MATINDNYLKLKAGYLFPEIGRRVSAFAEANPDAPIIKLGIGDVTEPLPEACRSAMVTAVEDMGNRDSFKGYGPEQGYGW
LREKIAAHDFQARGCDVDAGEIFISDGSKCDCGNILDIFGDNNTIAVTDPVYPVYVDTNVMAGHTGPCNDQGEYEGLTYL
PINAGNNFTAQIPSQKVDLIYLCFPNNPTGAVASKSHLQDWVNYAKSHGSIILFDAAYEAFITDPEIPHSIYEIEGARDC
AIEFRSFSKNAGFTGTRCALTVVPKTLMAKAADESDVELWKLWNRRQSTKFNGVSYIVQRGAEAVYSEAGQAQTKALISF
YLENAKIIREKLTAAGLQVFGGVNAPYVWVQTPNGISSWDFFDQLLHKTNVVGTPGSGFGAAGEGYFRISAFNSRANVEE
AMRRITANL

Sequences:

>Translated_409_residues
MATINDNYLKLKAGYLFPEIGRRVSAFAEANPDAPIIKLGIGDVTEPLPEACRSAMVTAVEDMGNRDSFKGYGPEQGYGW
LREKIAAHDFQARGCDVDAGEIFISDGSKCDCGNILDIFGDNNTIAVTDPVYPVYVDTNVMAGHTGPCNDQGEYEGLTYL
PINAGNNFTAQIPSQKVDLIYLCFPNNPTGAVASKSHLQDWVNYAKSHGSIILFDAAYEAFITDPEIPHSIYEIEGARDC
AIEFRSFSKNAGFTGTRCALTVVPKTLMAKAADESDVELWKLWNRRQSTKFNGVSYIVQRGAEAVYSEAGQAQTKALISF
YLENAKIIREKLTAAGLQVFGGVNAPYVWVQTPNGISSWDFFDQLLHKTNVVGTPGSGFGAAGEGYFRISAFNSRANVEE
AMRRITANL
>Mature_408_residues
ATINDNYLKLKAGYLFPEIGRRVSAFAEANPDAPIIKLGIGDVTEPLPEACRSAMVTAVEDMGNRDSFKGYGPEQGYGWL
REKIAAHDFQARGCDVDAGEIFISDGSKCDCGNILDIFGDNNTIAVTDPVYPVYVDTNVMAGHTGPCNDQGEYEGLTYLP
INAGNNFTAQIPSQKVDLIYLCFPNNPTGAVASKSHLQDWVNYAKSHGSIILFDAAYEAFITDPEIPHSIYEIEGARDCA
IEFRSFSKNAGFTGTRCALTVVPKTLMAKAADESDVELWKLWNRRQSTKFNGVSYIVQRGAEAVYSEAGQAQTKALISFY
LENAKIIREKLTAAGLQVFGGVNAPYVWVQTPNGISSWDFFDQLLHKTNVVGTPGSGFGAAGEGYFRISAFNSRANVEEA
MRRITANL

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily

Homologues:

Organism=Escherichia coli, GI1788722, Length=356, Percent_Identity=25.2808988764045, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPAT_ACAM1 (B0CDH5)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516214.1
- ProteinModelPortal:   B0CDH5
- SMR:   B0CDH5
- GeneID:   5680696
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1880
- HOGENOM:   HBG645860
- OMA:   VDLIYLC
- ProtClustDB:   PRK07590
- BioCyc:   AMAR329726:AM1_1880-MONOMER
- HAMAP:   MF_01642
- InterPro:   IPR004839
- InterPro:   IPR019942
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03542

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.83

Molecular weight: Translated: 44620; Mature: 44489

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 42-42 BINDING 72-72 BINDING 75-75 BINDING 109-109 BINDING 132-132 BINDING 187-187 BINDING 187-187 BINDING 215-215 BINDING 218-218 BINDING 246-246 BINDING 248-248 BINDING 257-257 BINDING 292-292 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATINDNYLKLKAGYLFPEIGRRVSAFAEANPDAPIIKLGIGDVTEPLPEACRSAMVTAV
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
EDMGNRDSFKGYGPEQGYGWLREKIAAHDFQARGCDVDAGEIFISDGSKCDCGNILDIFG
HHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEC
DNNTIAVTDPVYPVYVDTNVMAGHTGPCNDQGEYEGLTYLPINAGNNFTAQIPSQKVDLI
CCCEEEEECCEEEEEEECCEEECCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCEE
YLCFPNNPTGAVASKSHLQDWVNYAKSHGSIILFDAAYEAFITDPEIPHSIYEIEGARDC
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHEECCCCCCCHHEEECCCHHH
AIEFRSFSKNAGFTGTRCALTVVPKTLMAKAADESDVELWKLWNRRQSTKFNGVSYIVQR
HHHHHHCCCCCCCCCCEEEEEECCHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHH
GAEAVYSEAGQAQTKALISFYLENAKIIREKLTAAGLQVFGGVNAPYVWVQTPNGISSWD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHH
FFDQLLHKTNVVGTPGSGFGAAGEGYFRISAFNSRANVEEAMRRITANL
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ATINDNYLKLKAGYLFPEIGRRVSAFAEANPDAPIIKLGIGDVTEPLPEACRSAMVTAV
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
EDMGNRDSFKGYGPEQGYGWLREKIAAHDFQARGCDVDAGEIFISDGSKCDCGNILDIFG
HHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEC
DNNTIAVTDPVYPVYVDTNVMAGHTGPCNDQGEYEGLTYLPINAGNNFTAQIPSQKVDLI
CCCEEEEECCEEEEEEECCEEECCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCEE
YLCFPNNPTGAVASKSHLQDWVNYAKSHGSIILFDAAYEAFITDPEIPHSIYEIEGARDC
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHEECCCCCCCHHEEECCCHHH
AIEFRSFSKNAGFTGTRCALTVVPKTLMAKAADESDVELWKLWNRRQSTKFNGVSYIVQR
HHHHHHCCCCCCCCCCEEEEEECCHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHH
GAEAVYSEAGQAQTKALISFYLENAKIIREKLTAAGLQVFGGVNAPYVWVQTPNGISSWD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHH
FFDQLLHKTNVVGTPGSGFGAAGEGYFRISAFNSRANVEEAMRRITANL
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA