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The map label for this gene is ilvB [H]

Identifier: 158335027

GI number: 158335027

Start: 1848993

End: 1850630

Strand: Direct

Name: ilvB [H]

Synonym: AM1_1865

Alternate gene names: 158335027

Gene position: 1848993-1850630 (Clockwise)

Preceding gene: 158335026

Following gene: 158335028

Centisome position: 28.43

GC content: 48.6

Gene sequence:

>1638_bases
ATGAATACAGCAGAATTGTTGGTCAAATGTTTGGAAAACGAGGGTGTTGAGTATATTTTTGGGTTGCCAGGAGAAGAAAA
CCTCCAAGTCTTAGAAGCCCTGCGAGACTCCTCGATCCAGTTCATCACCACCCGCCATGAACAGGGGGCTGCGTTTATGG
CCGATGTATACGGTCGATTAACCGGCCGAGCAGGAGTTTGCCTTTCAACCCTAGGCCCAGGGGCCACCAACTTGATGACG
GGGGTTGCCGATGCCAACTTAGATGGAGCCCCCTTAGTGGCGATTACCGGGCAAGTCGGCACCGATCGCATGCATATCGA
GTCTCACCAGTATTTAGATTTGGTGGCCATGTTTAATCCCGTCACTAAATGGAACTCACAAATTGTTCGCCCCAGCAACA
CCCCAGAAATTGTGCGACGAGCCTTCAAACGGGCCCAAACAGAAAAACCGGGAGCGGTGCATATCGATTTACCGGAAAAT
ATTGCTGCTATGCCCGCCGAAGGCATGCCCCTACAGCAGGATAGCCTGAAGCGGAGTTTTGCCTCTCCTCGTAACCTTGA
GCAGGCAGCTGAAATCATTTCTCAGGCCACTAATCCTTTAATCTTGGTGGGCAATGGAGCCATTCGAGCCAATGCCAGCG
AGGCCCTCACCCAATTTGCCACCGAATTGAATATTCCTGTAGCCAATACGTTTATGGGTAAAGGGGTGATTCGCTATACC
CATCCCCTAGCTCTGTGGACCGTGGGATTACAACAGCGAGACTATATCAGCTGTGGCTTTGATCGAACCGATCTCGTCAT
TGCCGTGGGTTATGACTTAATCGAGTTTTCGCCTAAAAAGTGGAATCCGGATGGCACCATCCCCATTGTGCATGTGGGAG
CAGAGCCAGCTGAGATTGACAGTAGCTATATCCCTAAAACTGAAGTGGTCGGGGATATCTCTGATTCTCTGATCGAAATT
CTCCGACGGGCAGACCGCCAGGGCAAGCCCGATCCCTACGGGGTTGAGCTTAGACCTCAAATCCGCGCAGACTATGAGGA
ATATGCCAAGGACGAGCACTTTCCCTTACGTCCGCAAAAGCTGATTTATGATCTCCGCGAAGTTCTCGCATCCGAAGATA
TTGTGATCTCTGATGTGGGGGCTCACAAAATGTGGATGGCTCGCCATTATCATTGTGATCGCCCGAACACCTGCCTAATT
TCCAATGGATTTGCAGCGATGGGAATTGCCATTCCTGGCGCTTTAGCTGCCAAATTAGTCTATCCCGATCGCCATGTGGT
GGCTGTAACTGGCGACGGTGGTTTCATGATCAATATGCAGGAACTGGAAACAGCATTGCGTCTGGGTGTGGCTTTTACCA
CCATTATTTTTAACGATGGCGGCTATGGTTTGATTGAGTGGAAACAACAACAATATTACGGAGAAGCTTCTTTTATTAAG
TTTGGCAACCCTGATTTTGTGCGGCTCGCAGAAAGTATGGGACTCAAAGGTTATAGAGTAGAATCGAGTGCTGATTTTAT
GCCTTGTCTAAAGACTGCATTGGCACAAGATGTGCCAACAATTGTGGACTGTCCAATTGATTACAAAGAGAATTTAATGT
TTAGCCAAAAATCAGGTGAGATTGCTTGTATTAACTAA

Upstream 100 bases:

>100_bases
GGTGGCATCAAACGCTCTGGCTATGGCCGAGAATTAGGCATCCAAGGCATTCATGAATTCGTTAACATCAAAACCGTATG
GGTGAAGTGAGCCATTTTTA

Downstream 100 bases:

>100_bases
ACTTTTTATCCAGGGTAGAGAAACATTTGTTTGAGTTTTGTAGGAAACGATCATTTTTTAATTCATCAGGGATTAGGATT
TGATCGACAGCCCGGTTAGG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNTAELLVKCLENEGVEYIFGLPGEENLQVLEALRDSSIQFITTRHEQGAAFMADVYGRLTGRAGVCLSTLGPGATNLMT
GVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFNPVTKWNSQIVRPSNTPEIVRRAFKRAQTEKPGAVHIDLPEN
IAAMPAEGMPLQQDSLKRSFASPRNLEQAAEIISQATNPLILVGNGAIRANASEALTQFATELNIPVANTFMGKGVIRYT
HPLALWTVGLQQRDYISCGFDRTDLVIAVGYDLIEFSPKKWNPDGTIPIVHVGAEPAEIDSSYIPKTEVVGDISDSLIEI
LRRADRQGKPDPYGVELRPQIRADYEEYAKDEHFPLRPQKLIYDLREVLASEDIVISDVGAHKMWMARHYHCDRPNTCLI
SNGFAAMGIAIPGALAAKLVYPDRHVVAVTGDGGFMINMQELETALRLGVAFTTIIFNDGGYGLIEWKQQQYYGEASFIK
FGNPDFVRLAESMGLKGYRVESSADFMPCLKTALAQDVPTIVDCPIDYKENLMFSQKSGEIACIN

Sequences:

>Translated_545_residues
MNTAELLVKCLENEGVEYIFGLPGEENLQVLEALRDSSIQFITTRHEQGAAFMADVYGRLTGRAGVCLSTLGPGATNLMT
GVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFNPVTKWNSQIVRPSNTPEIVRRAFKRAQTEKPGAVHIDLPEN
IAAMPAEGMPLQQDSLKRSFASPRNLEQAAEIISQATNPLILVGNGAIRANASEALTQFATELNIPVANTFMGKGVIRYT
HPLALWTVGLQQRDYISCGFDRTDLVIAVGYDLIEFSPKKWNPDGTIPIVHVGAEPAEIDSSYIPKTEVVGDISDSLIEI
LRRADRQGKPDPYGVELRPQIRADYEEYAKDEHFPLRPQKLIYDLREVLASEDIVISDVGAHKMWMARHYHCDRPNTCLI
SNGFAAMGIAIPGALAAKLVYPDRHVVAVTGDGGFMINMQELETALRLGVAFTTIIFNDGGYGLIEWKQQQYYGEASFIK
FGNPDFVRLAESMGLKGYRVESSADFMPCLKTALAQDVPTIVDCPIDYKENLMFSQKSGEIACIN
>Mature_545_residues
MNTAELLVKCLENEGVEYIFGLPGEENLQVLEALRDSSIQFITTRHEQGAAFMADVYGRLTGRAGVCLSTLGPGATNLMT
GVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFNPVTKWNSQIVRPSNTPEIVRRAFKRAQTEKPGAVHIDLPEN
IAAMPAEGMPLQQDSLKRSFASPRNLEQAAEIISQATNPLILVGNGAIRANASEALTQFATELNIPVANTFMGKGVIRYT
HPLALWTVGLQQRDYISCGFDRTDLVIAVGYDLIEFSPKKWNPDGTIPIVHVGAEPAEIDSSYIPKTEVVGDISDSLIEI
LRRADRQGKPDPYGVELRPQIRADYEEYAKDEHFPLRPQKLIYDLREVLASEDIVISDVGAHKMWMARHYHCDRPNTCLI
SNGFAAMGIAIPGALAAKLVYPDRHVVAVTGDGGFMINMQELETALRLGVAFTTIIFNDGGYGLIEWKQQQYYGEASFIK
FGNPDFVRLAESMGLKGYRVESSADFMPCLKTALAQDVPTIVDCPIDYKENLMFSQKSGEIACIN

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:
GO:0000287: Acetolactate synthase, large subunit, biosynthetic type
GO:0003824: Acetolactate synthase, large subunit, biosynthetic type
GO:0008152: Acetolactate synthase, large subunit, biosynthetic type
GO:0016740: Acetolactate synthase, large subunit, biosynthetic type
GO:0030976: Acetolactate synthase, large subunit, biosynthetic type
GO:0003984: Acetolactate synthase
GO:0008652: Acetolactate synthase
GO:0009082: Acetolactate synthase
GO:0046872: Acetolactate synthase
GO:0050660: Acetolactate synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=555, Percent_Identity=25.4054054054054, Blast_Score=179, Evalue=7e-45,
Organism=Homo sapiens, GI21361361, Length=538, Percent_Identity=25.092936802974, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI87081685, Length=561, Percent_Identity=31.5508021390374, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790104, Length=537, Percent_Identity=30.1675977653631, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=28.141592920354, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1787096, Length=542, Percent_Identity=26.1992619926199, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788716, Length=545, Percent_Identity=26.605504587156, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=483, Percent_Identity=27.3291925465839, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=556, Percent_Identity=25.8992805755396, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=555, Percent_Identity=24.3243243243243, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=28.6219081272085, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=26.4344262295082, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323163, Length=523, Percent_Identity=22.5621414913958, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=528, Percent_Identity=23.1060606060606, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=519, Percent_Identity=22.7360308285164, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320123, Length=533, Percent_Identity=22.3264540337711, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922626, Length=557, Percent_Identity=27.1095152603232, Blast_Score=182, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59955; Mature: 59955

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAELLVKCLENEGVEYIFGLPGEENLQVLEALRDSSIQFITTRHEQGAAFMADVYGRL
CCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
TGRAGVCLSTLGPGATNLMTGVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFNP
CCCCCEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHCC
VTKWNSQIVRPSNTPEIVRRAFKRAQTEKPGAVHIDLPENIAAMPAEGMPLQQDSLKRSF
HHHCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCCCCCCCHHHHHHHH
ASPRNLEQAAEIISQATNPLILVGNGAIRANASEALTQFATELNIPVANTFMGKGVIRYT
CCCCCHHHHHHHHHHCCCCEEEEECCCEECCHHHHHHHHHHHCCCCCHHHHHCCCEEEEE
HPLALWTVGLQQRDYISCGFDRTDLVIAVGYDLIEFSPKKWNPDGTIPIVHVGAEPAEID
CCEEEEEECCCCCCCEECCCCCCCEEEEECCHHEECCCCCCCCCCCEEEEEECCCCHHCC
SSYIPKTEVVGDISDSLIEILRRADRQGKPDPYGVELRPQIRADYEEYAKDEHFPLRPQK
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHCCHHHHCCCCCCCCCHHH
LIYDLREVLASEDIVISDVGAHKMWMARHYHCDRPNTCLISNGFAAMGIAIPGALAAKLV
HHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCEEEEECCCEEEHHHCCCHHHHEEE
YPDRHVVAVTGDGGFMINMQELETALRLGVAFTTIIFNDGGYGLIEWKQQQYYGEASFIK
CCCCEEEEEECCCCEEEEHHHHHHHHHHHEEEEEEEECCCCCEEEEEHHHHHCCCEEEEE
FGNPDFVRLAESMGLKGYRVESSADFMPCLKTALAQDVPTIVDCPIDYKENLMFSQKSGE
CCCCHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCC
IACIN
EEEEC
>Mature Secondary Structure
MNTAELLVKCLENEGVEYIFGLPGEENLQVLEALRDSSIQFITTRHEQGAAFMADVYGRL
CCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
TGRAGVCLSTLGPGATNLMTGVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFNP
CCCCCEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHCC
VTKWNSQIVRPSNTPEIVRRAFKRAQTEKPGAVHIDLPENIAAMPAEGMPLQQDSLKRSF
HHHCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCCCCCCCHHHHHHHH
ASPRNLEQAAEIISQATNPLILVGNGAIRANASEALTQFATELNIPVANTFMGKGVIRYT
CCCCCHHHHHHHHHHCCCCEEEEECCCEECCHHHHHHHHHHHCCCCCHHHHHCCCEEEEE
HPLALWTVGLQQRDYISCGFDRTDLVIAVGYDLIEFSPKKWNPDGTIPIVHVGAEPAEID
CCEEEEEECCCCCCCEECCCCCCCEEEEECCHHEECCCCCCCCCCCEEEEEECCCCHHCC
SSYIPKTEVVGDISDSLIEILRRADRQGKPDPYGVELRPQIRADYEEYAKDEHFPLRPQK
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHCCHHHHCCCCCCCCCHHH
LIYDLREVLASEDIVISDVGAHKMWMARHYHCDRPNTCLISNGFAAMGIAIPGALAAKLV
HHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCEEEEECCCEEEHHHCCCHHHHEEE
YPDRHVVAVTGDGGFMINMQELETALRLGVAFTTIIFNDGGYGLIEWKQQQYYGEASFIK
CCCCEEEEEECCCCEEEEHHHHHHHHHHHEEEEEEEECCCCCEEEEEHHHHHCCCEEEEE
FGNPDFVRLAESMGLKGYRVESSADFMPCLKTALAQDVPTIVDCPIDYKENLMFSQKSGE
CCCCHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCC
IACIN
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]