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The map label for this gene is dacB [H]

Identifier: 158334994

GI number: 158334994

Start: 1819143

End: 1820609

Strand: Reverse

Name: dacB [H]

Synonym: AM1_1832

Alternate gene names: 158334994

Gene position: 1820609-1819143 (Counterclockwise)

Preceding gene: 158334998

Following gene: 158334993

Centisome position: 27.99

GC content: 51.4

Gene sequence:

>1467_bases
ATGTCTGGGGACAATGTTATTAAGGGTGGCTTGGTCGTTGGGCTGCTAAGTATGTCCGTCGCCGCCCAAGGTGCCCCATC
CCTGAAGCCCCAATGCGTGGCACAACTGCAGCGAGACATGGATGAGATCGCATCCCAACCCCTCTTCACTCAAGCTCGTT
TAGGGATCGCCGTTCAACCCCTTCAAGCCTCCCAACCGCTTTACACCAAGGACGCCCAGCGCTATTTCCTGCCCGCGTCT
ACCACCAAGGTGATGACGACAGCCGCGGCACTGACTCGGCTAGGTCCTCAATTTCGGATTCAGACCGATATTTATGGTTC
TGGGACAGGTCCTGCTCTTGACCATTTACGCATCGTAGGTCGAGGCGACCCCACCCTTTCTGATCAGCAGCTACAGCAAA
TCGCCACCCAACTTAAACAAAAAGGCATCCGCCAAATCAACCATCTCCTGGGAGATGACACCTATTTTCAGGGGCCAGGC
ATCGTTCCGTCCTGGGAATGGGAAGATCTGCAGGCGGGCTATGGCGCTCCCGTTAATAGCCTGATCCTCAATCAAAATGC
CTATGAGCTAAAAATCTGGCCCCAAGCACTAGGCCAGCCCCTCCGCGTCGAATGGCTTTCTCCTCACCCCATTGGCCCAT
GGCGACAGATCAATCGCACCCGCACCGTAGCTCAAAATGCCCCAGAATATATAGAAATTGTCCGCCGCCTCAAGGGAAAT
ACTTTAGAAATTGAGATTACAGGACAGCTGCGAGCCGGTGCCCCTCACGATCGCTCTGCCGTAGCCGTTATTGATCCAGG
CTGGTTTTTACTCAAGCGATTTCGGGCCATCTTGGCGGCTCAACAGATTAAAGTAGGGCGATTATCACTTCTATCGACCC
CTCAAGAACGCAATGCTGCTTTAAAAGGCAAATCAGAACAGCGCCTCACGCAGATCCAATCGTCGCCTCTGACAACGCTG
ATTAAAATTACGAACCAAGAGAGCAATAATCTTTACGCAGAAGCTCTAGTCAAAGCCTTGGGGGCAGCCCAAAAGCAAAC
TTCACAAAATACCCATCAGCAAGGTTTAAAGGCTTTAAAACAAACTCTCACCCAACTAGGGGTCAATGCGTCCACCTATG
CAGTGGCCGATGGCTCTGGTTTATCTCGACAAAACTTGGTCAGCCCCCTGGCCCTAACTCAAACTTTAAATGGCATTCTC
AAATCTCCCCACGCCTCTACCTTTCAGACGTCTTTACCTAAAGACTCTATTGGGCAAAATTCACCCTCAGCCCTCTGGAG
TAAAAGTGGGGGGATGCGCGGGGTGTCGACCTTAACGGGCTACCTCCAACACCCTCAATCTTCACCGGTACTGTTTAGTC
TGATGTTTAATCAATACAATCAATCGAGCAGCAAACGGCGGCAAGCTATGGATGCCATGCTGTTATCAATCAATCGTTGG
CAATCTTGTCAGGGTAGAAACGGATGA

Upstream 100 bases:

>100_bases
AGAAAGGGATTGAAGACATCAATCCTAAAGTCGGTCTGAAGGCTGAATAACCCCTTCTCAATTCACGTTAAGCTGGCTCC
TATCATCAGGAGAGCATGGA

Downstream 100 bases:

>100_bases
CTTCTCTATCGCCTCCCCCCTCCACCCAACAGGTGGTTCTCGCGGTTTCCGGTATGAAATGCGCAGGATGCGTCCAGGCC
GTGGAAAAACGCCTGCAGCA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein; PBP [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSGDNVIKGGLVVGLLSMSVAAQGAPSLKPQCVAQLQRDMDEIASQPLFTQARLGIAVQPLQASQPLYTKDAQRYFLPAS
TTKVMTTAAALTRLGPQFRIQTDIYGSGTGPALDHLRIVGRGDPTLSDQQLQQIATQLKQKGIRQINHLLGDDTYFQGPG
IVPSWEWEDLQAGYGAPVNSLILNQNAYELKIWPQALGQPLRVEWLSPHPIGPWRQINRTRTVAQNAPEYIEIVRRLKGN
TLEIEITGQLRAGAPHDRSAVAVIDPGWFLLKRFRAILAAQQIKVGRLSLLSTPQERNAALKGKSEQRLTQIQSSPLTTL
IKITNQESNNLYAEALVKALGAAQKQTSQNTHQQGLKALKQTLTQLGVNASTYAVADGSGLSRQNLVSPLALTQTLNGIL
KSPHASTFQTSLPKDSIGQNSPSALWSKSGGMRGVSTLTGYLQHPQSSPVLFSLMFNQYNQSSSKRRQAMDAMLLSINRW
QSCQGRNG

Sequences:

>Translated_488_residues
MSGDNVIKGGLVVGLLSMSVAAQGAPSLKPQCVAQLQRDMDEIASQPLFTQARLGIAVQPLQASQPLYTKDAQRYFLPAS
TTKVMTTAAALTRLGPQFRIQTDIYGSGTGPALDHLRIVGRGDPTLSDQQLQQIATQLKQKGIRQINHLLGDDTYFQGPG
IVPSWEWEDLQAGYGAPVNSLILNQNAYELKIWPQALGQPLRVEWLSPHPIGPWRQINRTRTVAQNAPEYIEIVRRLKGN
TLEIEITGQLRAGAPHDRSAVAVIDPGWFLLKRFRAILAAQQIKVGRLSLLSTPQERNAALKGKSEQRLTQIQSSPLTTL
IKITNQESNNLYAEALVKALGAAQKQTSQNTHQQGLKALKQTLTQLGVNASTYAVADGSGLSRQNLVSPLALTQTLNGIL
KSPHASTFQTSLPKDSIGQNSPSALWSKSGGMRGVSTLTGYLQHPQSSPVLFSLMFNQYNQSSSKRRQAMDAMLLSINRW
QSCQGRNG
>Mature_487_residues
SGDNVIKGGLVVGLLSMSVAAQGAPSLKPQCVAQLQRDMDEIASQPLFTQARLGIAVQPLQASQPLYTKDAQRYFLPAST
TKVMTTAAALTRLGPQFRIQTDIYGSGTGPALDHLRIVGRGDPTLSDQQLQQIATQLKQKGIRQINHLLGDDTYFQGPGI
VPSWEWEDLQAGYGAPVNSLILNQNAYELKIWPQALGQPLRVEWLSPHPIGPWRQINRTRTVAQNAPEYIEIVRRLKGNT
LEIEITGQLRAGAPHDRSAVAVIDPGWFLLKRFRAILAAQQIKVGRLSLLSTPQERNAALKGKSEQRLTQIQSSPLTTLI
KITNQESNNLYAEALVKALGAAQKQTSQNTHQQGLKALKQTLTQLGVNASTYAVADGSGLSRQNLVSPLALTQTLNGILK
SPHASTFQTSLPKDSIGQNSPSALWSKSGGMRGVSTLTGYLQHPQSSPVLFSLMFNQYNQSSSKRRQAMDAMLLSINRWQ
SCQGRNG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:
GO:0004180: D-alanyl-D-alanine carboxypeptidase, putative
GO:0004185: D-alanyl-D-alanine carboxypeptidase, putative
GO:0006508: D-alanyl-D-alanine carboxypeptidase, putative
GO:0008233: D-alanyl-D-alanine carboxypeptidase, putative

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=425, Percent_Identity=28.2352941176471, Blast_Score=126, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 53235; Mature: 53103

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDNVIKGGLVVGLLSMSVAAQGAPSLKPQCVAQLQRDMDEIASQPLFTQARLGIAVQP
CCCCCEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEC
LQASQPLYTKDAQRYFLPASTTKVMTTAAALTRLGPQFRIQTDIYGSGTGPALDHLRIVG
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHEEEEE
RGDPTLSDQQLQQIATQLKQKGIRQINHLLGDDTYFQGPGIVPSWEWEDLQAGYGAPVNS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHCCCCCCCHHH
LILNQNAYELKIWPQALGQPLRVEWLSPHPIGPWRQINRTRTVAQNAPEYIEIVRRLKGN
HEECCCCEEEEECHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCC
TLEIEITGQLRAGAPHDRSAVAVIDPGWFLLKRFRAILAAQQIKVGRLSLLSTPQERNAA
EEEEEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LKGKSEQRLTQIQSSPLTTLIKITNQESNNLYAEALVKALGAAQKQTSQNTHQQGLKALK
HCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTLTQLGVNASTYAVADGSGLSRQNLVSPLALTQTLNGILKSPHASTFQTSLPKDSIGQN
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHCCCC
SPSALWSKSGGMRGVSTLTGYLQHPQSSPVLFSLMFNQYNQSSSKRRQAMDAMLLSINRW
CCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QSCQGRNG
HCCCCCCC
>Mature Secondary Structure 
SGDNVIKGGLVVGLLSMSVAAQGAPSLKPQCVAQLQRDMDEIASQPLFTQARLGIAVQP
CCCCEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEC
LQASQPLYTKDAQRYFLPASTTKVMTTAAALTRLGPQFRIQTDIYGSGTGPALDHLRIVG
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHEEEEE
RGDPTLSDQQLQQIATQLKQKGIRQINHLLGDDTYFQGPGIVPSWEWEDLQAGYGAPVNS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHCCCCCCCHHH
LILNQNAYELKIWPQALGQPLRVEWLSPHPIGPWRQINRTRTVAQNAPEYIEIVRRLKGN
HEECCCCEEEEECHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCC
TLEIEITGQLRAGAPHDRSAVAVIDPGWFLLKRFRAILAAQQIKVGRLSLLSTPQERNAA
EEEEEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LKGKSEQRLTQIQSSPLTTLIKITNQESNNLYAEALVKALGAAQKQTSQNTHQQGLKALK
HCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTLTQLGVNASTYAVADGSGLSRQNLVSPLALTQTLNGILKSPHASTFQTSLPKDSIGQN
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHCCCC
SPSALWSKSGGMRGVSTLTGYLQHPQSSPVLFSLMFNQYNQSSSKRRQAMDAMLLSINRW
CCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QSCQGRNG
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1554361; 7305936 [H]