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The map label for this gene is rpoD [H]

Identifier: 158334991

GI number: 158334991

Start: 1815011

End: 1816126

Strand: Reverse

Name: rpoD [H]

Synonym: AM1_1829

Alternate gene names: 158334991

Gene position: 1816126-1815011 (Counterclockwise)

Preceding gene: 158334993

Following gene: 158334988

Centisome position: 27.92

GC content: 48.66

Gene sequence:

>1116_bases
ATGACCCAAGCCAATAATGTACTAGAAGCTATCCAAGCTCCGGCTAATGATTTAGATAATCTGGTTGAGGATGCTGCCAG
TAGCACTAGTGAGGATAACCAAGCTGGCGAAGGTGGGAAAACGACAAAAGCTCGTACTGCCCGTCGACGCGGTCAATCCA
AGAAGAAGCATTACACGGAAGATTCCATCCGGCTGTATCTACAAGAAATCGGTAGAATTCGATTACTCCGAGCTGATGAA
GAGATTGAACTGGCTCGTAAAATTGCAGACTTGCTAGAGCTAGAGCGCATTTATGACGATCTGTTTGATCGACTAGAGCG
TGAGCCTCAGCTTCAAGAGTGGGCATTAGAAGTGGATATGCCCCTTAACCGTTTCCGCCATCGTCTGCATGCAGGCCGCA
GAGCTAAAGACAAGATGGTCCAGTCCAACCTCCGATTAGTCGTATCCATCGCTAAGAAATATATGAATCGGGGCCTGTCT
TTCCAGGACTTGATTCAAGAGGGTAGCCTGGGTTTGATCCGGGCCGCTGAAAAGTTTGACCACGAGAAAGGATACAAGTT
TTCCACCTATGCTACCTGGTGGATTCGTCAGGCGATCACCCGTGCGATCGCGGATCAATCCCGCACGATTCGCCTCCCCG
TGCACCTGTACGAAACTATTTCTCGCATTAAGAAAACCACAAAATTGCTGTCCCAAGAAATGGGTCGCAAGCCTACGGAA
GAAGAAATTGCCACTCGCATGGAAATGACCATCGAGAAGCTGCGTTTCATCGCCAAGTCCGCCCAATTACCCATCTCTTT
AGAAACCCCCATTGGTAAAGAAGAAGACTCCCGCTTAGGTGACTTTATCGAGTCCGATGGTGAGACGCCTGAGGATCAGG
TATCCAAGAGCTTACTTCGAGAAGATCTAGAAACGGTTCTAGATACCCTCAGCCCTCGTGAGCGTGATGTGTTGAAGTTA
CGCTACGGCCTAGATGATGGTCGAATGAAGACCCTAGAAGAAATTGGCCAGATCTTCAATGTTACCCGTGAGCGGATTCG
CCAAATTGAAGCCAAGGCTTTGCGCAAGTTGCGCCATCCTAACCGCAATAGTGTTTTGAAAGAGTATATCCGCTAG

Upstream 100 bases:

>100_bases
ATCTAGATCCACATCCCTAACTGAATTTGTTAGATTCGGTTGTAGTGAGTAAAAAATGGTTCTCCTATCGTCTTAATTAG
GGAGTATTGAGGAATCCGGC

Downstream 100 bases:

>100_bases
TTTCAATCATTCATCCATCAAGAAAGAGGCTGCTGTTCTTCAAATGGCAGCCTCTTTCTCATGACAACATTATGATCAAT
TAAACAGCTAGACTTCCTTT

Product: RNA polymerase sigma-70 factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTQANNVLEAIQAPANDLDNLVEDAASSTSEDNQAGEGGKTTKARTARRRGQSKKKHYTEDSIRLYLQEIGRIRLLRADE
EIELARKIADLLELERIYDDLFDRLEREPQLQEWALEVDMPLNRFRHRLHAGRRAKDKMVQSNLRLVVSIAKKYMNRGLS
FQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPTE
EEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGETPEDQVSKSLLREDLETVLDTLSPRERDVLKL
RYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR

Sequences:

>Translated_371_residues
MTQANNVLEAIQAPANDLDNLVEDAASSTSEDNQAGEGGKTTKARTARRRGQSKKKHYTEDSIRLYLQEIGRIRLLRADE
EIELARKIADLLELERIYDDLFDRLEREPQLQEWALEVDMPLNRFRHRLHAGRRAKDKMVQSNLRLVVSIAKKYMNRGLS
FQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPTE
EEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGETPEDQVSKSLLREDLETVLDTLSPRERDVLKL
RYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR
>Mature_370_residues
TQANNVLEAIQAPANDLDNLVEDAASSTSEDNQAGEGGKTTKARTARRRGQSKKKHYTEDSIRLYLQEIGRIRLLRADEE
IELARKIADLLELERIYDDLFDRLEREPQLQEWALEVDMPLNRFRHRLHAGRRAKDKMVQSNLRLVVSIAKKYMNRGLSF
QDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPTEE
EIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGETPEDQVSKSLLREDLETVLDTLSPRERDVLKLR
YGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=240, Percent_Identity=59.5833333333333, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1789098, Length=299, Percent_Identity=38.7959866220736, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1789871, Length=248, Percent_Identity=28.6290322580645, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=29.064039408867, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 43087; Mature: 42956

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQANNVLEAIQAPANDLDNLVEDAASSTSEDNQAGEGGKTTKARTARRRGQSKKKHYTE
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCH
DSIRLYLQEIGRIRLLRADEEIELARKIADLLELERIYDDLFDRLEREPQLQEWALEVDM
HHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
PLNRFRHRLHAGRRAKDKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFD
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHH
HEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPTE
HHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGETPEDQVSKSLLR
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
EDLETVLDTLSPRERDVLKLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHP
HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRNSVLKEYIR
CHHHHHHHHHC
>Mature Secondary Structure 
TQANNVLEAIQAPANDLDNLVEDAASSTSEDNQAGEGGKTTKARTARRRGQSKKKHYTE
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCH
DSIRLYLQEIGRIRLLRADEEIELARKIADLLELERIYDDLFDRLEREPQLQEWALEVDM
HHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
PLNRFRHRLHAGRRAKDKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFD
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHH
HEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISRIKKTTKLLSQEMGRKPTE
HHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGETPEDQVSKSLLR
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
EDLETVLDTLSPRERDVLKLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHP
HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRNSVLKEYIR
CHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]