Click here to switch to the map view.

The map label for this gene is trmJ [H]

Identifier: 158334964

GI number: 158334964

Start: 1791691

End: 1792443

Strand: Direct

Name: trmJ [H]

Synonym: AM1_1801

Alternate gene names: 158334964

Gene position: 1791691-1792443 (Clockwise)

Preceding gene: 158334954

Following gene: 158334967

Centisome position: 27.55

GC content: 50.2

Gene sequence:

>753_bases
ATGAGCGAACCACGGTTAGCTTCGATCAAAATTGTTTTAGTCGAACCGGCTGGCCCCCTCAATGTGGGGAGCATCGCCCG
AGTGATGAAAAACATGGGATTAGGGCAGTTAGTGCTCGTTAAACCCCACTGTAATCCTTTAGGCCCAGAAGCCAAACAGA
TGTCGGTTCACGCCGTTGACGTCCTATACCAGGCTAAAACGGTTCAAACGTTATCGGAAGCACTCGTGGGATGTCAGCGA
GTGGTGGCTACCACCGCTCGACAACGGCGGTTACAGGTGGTAACTCAAGCCCCTAAACAAATCTTGCCCTGGATACTAGA
AGGTGAAGCCGCCTTGATCTTTGGCCCCGAGGATCGAGGGCTCAGCAATGAAGAGCTTTACCACGCCCAAGCTTTCCTTC
AAATCCCGACAAGCTCTGACTATGCAGCCTTGAATCTCGCTCAGGCCGTCACAATTTGCTGTTACGAGTTGCGTCAATGC
CTACTGATGGGCCTTGACAACCGGCCCACTGAGCCTAGAATTGCAGAGTCTGATCATCTCCAAGTAGAATTGGCCCCCTT
TCAACAAATGGAAGCGTTCTATCAAGCCCTAGAAGTAGCTTTATTGGACATCGGCTATCTACATCCTCATACAGCAACCG
CAAGGATGCAGAAACTACGGCAGCTCTTTAATCGCAGTCAGCTGTCAACCCAGGAATTAGCTATGTTAAGAGGGATGATT
CGTCAGGTACACTGGAAAACTCAGGCAAATTGA

Upstream 100 bases:

>100_bases
GAACGCCCCACTAACAATTTGCAAAATCCGAATATGTAATAGTCGGCATGGTATTGTTTGCGATCAAATCTCCAAGACAT
CCACCTGAGTTAAGTTACCC

Downstream 100 bases:

>100_bases
TTAAAATACTCATAATCTTTTACTGTCGGCAATGCATTAAAGATTGAATAAAACTTTGAGGCTTGCCGATCCAGAGTGGA
TACAGCTTCAAGTCCTCAGC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSEPRLASIKIVLVEPAGPLNVGSIARVMKNMGLGQLVLVKPHCNPLGPEAKQMSVHAVDVLYQAKTVQTLSEALVGCQR
VVATTARQRRLQVVTQAPKQILPWILEGEAALIFGPEDRGLSNEELYHAQAFLQIPTSSDYAALNLAQAVTICCYELRQC
LLMGLDNRPTEPRIAESDHLQVELAPFQQMEAFYQALEVALLDIGYLHPHTATARMQKLRQLFNRSQLSTQELAMLRGMI
RQVHWKTQAN

Sequences:

>Translated_250_residues
MSEPRLASIKIVLVEPAGPLNVGSIARVMKNMGLGQLVLVKPHCNPLGPEAKQMSVHAVDVLYQAKTVQTLSEALVGCQR
VVATTARQRRLQVVTQAPKQILPWILEGEAALIFGPEDRGLSNEELYHAQAFLQIPTSSDYAALNLAQAVTICCYELRQC
LLMGLDNRPTEPRIAESDHLQVELAPFQQMEAFYQALEVALLDIGYLHPHTATARMQKLRQLFNRSQLSTQELAMLRGMI
RQVHWKTQAN
>Mature_249_residues
SEPRLASIKIVLVEPAGPLNVGSIARVMKNMGLGQLVLVKPHCNPLGPEAKQMSVHAVDVLYQAKTVQTLSEALVGCQRV
VATTARQRRLQVVTQAPKQILPWILEGEAALIFGPEDRGLSNEELYHAQAFLQIPTSSDYAALNLAQAVTICCYELRQCL
LMGLDNRPTEPRIAESDHLQVELAPFQQMEAFYQALEVALLDIGYLHPHTATARMQKLRQLFNRSQLSTQELAMLRGMIR
QVHWKTQAN

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=252, Percent_Identity=36.9047619047619, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1790865, Length=154, Percent_Identity=38.961038961039, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27857; Mature: 27726

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPRLASIKIVLVEPAGPLNVGSIARVMKNMGLGQLVLVKPHCNPLGPEAKQMSVHAVD
CCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHH
VLYQAKTVQTLSEALVGCQRVVATTARQRRLQVVTQAPKQILPWILEGEAALIFGPEDRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCEEEEECCCCCC
LSNEELYHAQAFLQIPTSSDYAALNLAQAVTICCYELRQCLLMGLDNRPTEPRIAESDHL
CCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
QVELAPFQQMEAFYQALEVALLDIGYLHPHTATARMQKLRQLFNRSQLSTQELAMLRGMI
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RQVHWKTQAN
HHHHHHCCCC
>Mature Secondary Structure 
SEPRLASIKIVLVEPAGPLNVGSIARVMKNMGLGQLVLVKPHCNPLGPEAKQMSVHAVD
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHH
VLYQAKTVQTLSEALVGCQRVVATTARQRRLQVVTQAPKQILPWILEGEAALIFGPEDRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCEEEEECCCCCC
LSNEELYHAQAFLQIPTSSDYAALNLAQAVTICCYELRQCLLMGLDNRPTEPRIAESDHL
CCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
QVELAPFQQMEAFYQALEVALLDIGYLHPHTATARMQKLRQLFNRSQLSTQELAMLRGMI
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RQVHWKTQAN
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA