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The map label for this gene is tufB

Identifier: 158334957

GI number: 158334957

Start: 1783826

End: 1785055

Strand: Reverse

Name: tufB

Synonym: AM1_1794

Alternate gene names: 158334957

Gene position: 1785055-1783826 (Counterclockwise)

Preceding gene: 158334958

Following gene: 158334956

Centisome position: 27.45

GC content: 47.48

Gene sequence:

>1230_bases
ATGGCACGCGCAAAATTTGAACGAACAAAACCCCACGTCAACATTGGCACCATTGGTCACGTTGATCATGGTAAAACCAC
TTTGACAGCAGCAATTACCATGTCTCTCGCTGCCCTTGGCCAAGCTAAAGCCAGGAAGTACGATGACATTGATGCGGCCC
CAGAAGAGCGGGAAAGAGGCATTACCATCAATACAGCTCACGTTGAGTATGAGACGCAAGACCGTCACTATGCCCACGTG
GACTGTCCTGGGCACGCTGACTATGTCAAGAACATGATTACTGGGGCTGCCCAGATGGACGGGGCAGTATTAGTTGTCTC
TGCTGCTGATGGACCCATGCCCCAAACTCGTGAACATATTCTTTTGGCTAAGCAAGTTGGGGTTCCCAACATTGTGGTCT
TTATGAATAAGCAAGACCAGGTAGATGATGAAGAGTTGCTGGAGTTGGTTGAGCTAGAAGTTCGAGAACTCTTAAACGAC
TATGATTTCCCAGGTGATGATATTCCCATCGTTTCAGGCTCTGCTCTAATGGCGTTGGAAGCGCTCAACGGTGCTGACTC
CATGAAAAAAGGGGATAACGAATGGGTGGATAAGATTTATAAGTTGATGGAAGAAGTGGATGCCTATATTCCTACTCCTG
AAAGAGATGTTGATAAGCCTTTCTTAATGGCTGTTGAAGATGTTTTCTCTATCACAGGTCGTGGAACTGTTGCGACTGGC
CGGATCGAGCGAGGCAAGGTTGTCGTTGGTGAGACCGTTGAGTTAGTTGGGATTAGAGATACACGCAGCACTACGGTGAC
TGGTGTTGAAATGTTCCAAAAAACCCTTGACGAAGGAATGGCTGGTGATAACGTTGGCTTGCTCTTGCGAGGTGTTCAGA
AAGAAGACATTGAGCGCGGCATGGTATTGGCTAAGCCGGGTTCCATTACCCCTCATACTCAATTTGAGTCTGAAGTCTAT
ATCCTTAAGAAGGATGAGGGAGGGCGTCACACACCGTTTTTCCCTGGCTACCGCCCTCAGTTCTATGTGAGAACAACAGA
TGTAACGGGTACAATCAGTGCTTTTACAGCAGATGATGGCAGTGCTGCTGAAATGGTTATGCCTGGTGATCGCATTAAAA
TGACGGTTGAATTAATCAACCCCATTGCGATTGAGCAAGGGATGCGATTTGCGATTCGTGAAGGTGGACGGACTGTTGGA
GCTGGTGTTGTCTCCAAAATCCTTAAATAG

Upstream 100 bases:

>100_bases
GAGTTTGGCAACTACGAGGACGTTCCTCGTAATGTGGCTGAACCCATTATCGAAAAAAACAAAGGGAACGCATAATTCAT
CGAAAAGGAATAGTTGATAC

Downstream 100 bases:

>100_bases
TCTGCTAAGCAGCCTTTAAGGATGAGCTGAATGCGGGCGCTATCGCCATACCTTCAGCTCATCCTTAAGGACTGAATTCC
AACAGCACTGCTATAAAGCT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MARAKFERTKPHVNIGTIGHVDHGKTTLTAAITMSLAALGQAKARKYDDIDAAPEERERGITINTAHVEYETQDRHYAHV
DCPGHADYVKNMITGAAQMDGAVLVVSAADGPMPQTREHILLAKQVGVPNIVVFMNKQDQVDDEELLELVELEVRELLND
YDFPGDDIPIVSGSALMALEALNGADSMKKGDNEWVDKIYKLMEEVDAYIPTPERDVDKPFLMAVEDVFSITGRGTVATG
RIERGKVVVGETVELVGIRDTRSTTVTGVEMFQKTLDEGMAGDNVGLLLRGVQKEDIERGMVLAKPGSITPHTQFESEVY
ILKKDEGGRHTPFFPGYRPQFYVRTTDVTGTISAFTADDGSAAEMVMPGDRIKMTVELINPIAIEQGMRFAIREGGRTVG
AGVVSKILK

Sequences:

>Translated_409_residues
MARAKFERTKPHVNIGTIGHVDHGKTTLTAAITMSLAALGQAKARKYDDIDAAPEERERGITINTAHVEYETQDRHYAHV
DCPGHADYVKNMITGAAQMDGAVLVVSAADGPMPQTREHILLAKQVGVPNIVVFMNKQDQVDDEELLELVELEVRELLND
YDFPGDDIPIVSGSALMALEALNGADSMKKGDNEWVDKIYKLMEEVDAYIPTPERDVDKPFLMAVEDVFSITGRGTVATG
RIERGKVVVGETVELVGIRDTRSTTVTGVEMFQKTLDEGMAGDNVGLLLRGVQKEDIERGMVLAKPGSITPHTQFESEVY
ILKKDEGGRHTPFFPGYRPQFYVRTTDVTGTISAFTADDGSAAEMVMPGDRIKMTVELINPIAIEQGMRFAIREGGRTVG
AGVVSKILK
>Mature_408_residues
ARAKFERTKPHVNIGTIGHVDHGKTTLTAAITMSLAALGQAKARKYDDIDAAPEERERGITINTAHVEYETQDRHYAHVD
CPGHADYVKNMITGAAQMDGAVLVVSAADGPMPQTREHILLAKQVGVPNIVVFMNKQDQVDDEELLELVELEVRELLNDY
DFPGDDIPIVSGSALMALEALNGADSMKKGDNEWVDKIYKLMEEVDAYIPTPERDVDKPFLMAVEDVFSITGRGTVATGR
IERGKVVVGETVELVGIRDTRSTTVTGVEMFQKTLDEGMAGDNVGLLLRGVQKEDIERGMVLAKPGSITPHTQFESEVYI
LKKDEGGRHTPFFPGYRPQFYVRTTDVTGTISAFTADDGSAAEMVMPGDRIKMTVELINPIAIEQGMRFAIREGGRTVGA
GVVSKILK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=407, Percent_Identity=53.8083538083538, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=30.2961275626424, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=30.2961275626424, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI4503475, Length=461, Percent_Identity=30.8026030368764, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI4503471, Length=457, Percent_Identity=29.3216630196937, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI194018522, Length=442, Percent_Identity=27.1493212669683, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI46094014, Length=443, Percent_Identity=27.5395033860045, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI194097354, Length=442, Percent_Identity=27.1493212669683, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI194018520, Length=442, Percent_Identity=27.1493212669683, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI54607086, Length=323, Percent_Identity=27.8637770897833, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4503507, Length=286, Percent_Identity=28.3216783216783, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=33.5820895522388, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI157426893, Length=297, Percent_Identity=24.5791245791246, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI18390331, Length=133, Percent_Identity=33.8345864661654, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=408, Percent_Identity=71.8137254901961, Blast_Score=569, Evalue=1e-164,
Organism=Escherichia coli, GI1789737, Length=408, Percent_Identity=71.8137254901961, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI2367247, Length=355, Percent_Identity=29.5774647887324, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994988, Length=245, Percent_Identity=32.2448979591837, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789108, Length=345, Percent_Identity=29.8550724637681, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790835, Length=149, Percent_Identity=30.2013422818792, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=147, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=412, Percent_Identity=49.5145631067961, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25141371, Length=400, Percent_Identity=43.25, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=30.1552106430155, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=30.1552106430155, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI32566629, Length=455, Percent_Identity=27.9120879120879, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=26.6514806378132, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=26.6514806378132, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32566303, Length=444, Percent_Identity=27.9279279279279, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=282, Percent_Identity=29.4326241134752, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=38.6206896551724, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=245, Percent_Identity=26.9387755102041, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=407, Percent_Identity=61.1793611793612, Blast_Score=508, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=29.3064876957494, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=29.3064876957494, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=460, Percent_Identity=25.2173913043478, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320377, Length=441, Percent_Identity=27.437641723356, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=290, Percent_Identity=26.8965517241379, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=392, Percent_Identity=25.765306122449, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=227, Percent_Identity=31.2775330396476, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363316, Length=403, Percent_Identity=56.8238213399504, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=403, Percent_Identity=56.8238213399504, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=402, Percent_Identity=47.2636815920398, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=442, Percent_Identity=28.5067873303167, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI45553816, Length=442, Percent_Identity=28.5067873303167, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24651721, Length=442, Percent_Identity=28.5067873303167, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI17864154, Length=442, Percent_Identity=28.5067873303167, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24652838, Length=444, Percent_Identity=28.3783783783784, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137572, Length=444, Percent_Identity=28.3783783783784, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45550900, Length=452, Percent_Identity=26.3274336283186, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI19922690, Length=313, Percent_Identity=30.3514376996805, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17137380, Length=445, Percent_Identity=27.8651685393258, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24647048, Length=277, Percent_Identity=27.4368231046931, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24647046, Length=277, Percent_Identity=27.4368231046931, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=34.4594594594595, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=35.9756097560976, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_ACAM1 (B0CCD0)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516129.1
- ProteinModelPortal:   B0CCD0
- SMR:   B0CCD0
- GeneID:   5680610
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1794
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- BioCyc:   AMAR329726:AM1_1794-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44775; Mature: 44643

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAKFERTKPHVNIGTIGHVDHGKTTLTAAITMSLAALGQAKARKYDDIDAAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
ITINTAHVEYETQDRHYAHVDCPGHADYVKNMITGAAQMDGAVLVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLAKQVGVPNIVVFMNKQDQVDDEELLELVELEVRELLNDYDFPGDDIPIVSGSALMALE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEE
ALNGADSMKKGDNEWVDKIYKLMEEVDAYIPTPERDVDKPFLMAVEDVFSITGRGTVATG
HHCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEC
RIERGKVVVGETVELVGIRDTRSTTVTGVEMFQKTLDEGMAGDNVGLLLRGVQKEDIERG
CCCCCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCC
MVLAKPGSITPHTQFESEVYILKKDEGGRHTPFFPGYRPQFYVRTTDVTGTISAFTADDG
EEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCC
SAAEMVMPGDRIKMTVELINPIAIEQGMRFAIREGGRTVGAGVVSKILK
CCEEEECCCCCEEEEEEHHCCHHHHHCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
ARAKFERTKPHVNIGTIGHVDHGKTTLTAAITMSLAALGQAKARKYDDIDAAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCC
ITINTAHVEYETQDRHYAHVDCPGHADYVKNMITGAAQMDGAVLVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLAKQVGVPNIVVFMNKQDQVDDEELLELVELEVRELLNDYDFPGDDIPIVSGSALMALE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEE
ALNGADSMKKGDNEWVDKIYKLMEEVDAYIPTPERDVDKPFLMAVEDVFSITGRGTVATG
HHCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEC
RIERGKVVVGETVELVGIRDTRSTTVTGVEMFQKTLDEGMAGDNVGLLLRGVQKEDIERG
CCCCCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCC
MVLAKPGSITPHTQFESEVYILKKDEGGRHTPFFPGYRPQFYVRTTDVTGTISAFTADDG
EEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCC
SAAEMVMPGDRIKMTVELINPIAIEQGMRFAIREGGRTVGAGVVSKILK
CCEEEECCCCCEEEEEEHHCCHHHHHCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA