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The map label for this gene is speE [H]

Identifier: 158334894

GI number: 158334894

Start: 1716867

End: 1718435

Strand: Direct

Name: speE [H]

Synonym: AM1_1731

Alternate gene names: 158334894

Gene position: 1716867-1718435 (Clockwise)

Preceding gene: 158334893

Following gene: 158334895

Centisome position: 26.4

GC content: 52.84

Gene sequence:

>1569_bases
ATGGCCGAGTCAGAATCAACCTCTGGGGGAGTCCAGAGGCCTGAATCTATTCCCCTCAGCTCTAGTCAGCAACGCTGGTT
ACTGGCAGCCGCTGCCATCTCTTCCAGTGTGGGCCTCGCCGCAGAATTACTCTTAGGAACCCTAGCCAGTTATTTAGTCG
GCAATACTGCATTAGCTTACGGTGTAGCCGTAGGAGGCTTTCTAGCTGCCATGGGATTGGGAGCCTATATCAGTCAATTT
ATTGCCGTAGACAATCAACAAAGCCAGTTAATCAAAGTTTTCTTACAAATTGAGTTGTGGGTTGCCCCATTAACAGCTGG
CTTACCCATTGGACTGTTTGTCGTTTTTGTCGCCGATGGACCGATTTGGCTAGGGCTCTTTCTTGTAACCCTTATTTTGG
GTATTCTATCGGGCCTAGAAGTCCCCATTTTGACTCGCATTATCGAACAAGATCGGGACGTCAAGTTCGCCCTTGCTGGA
GTCTTGGCCTTAGATTATGTTGGCGCTTTAATCGGTTCCTTGGCGTTTCCGGTCCTACTTCTGCCCCTGTTGGGATTATT
TCCCACCGCCGTGCTGATCGGGTCTTTACCTGCCTTTATGGTCTTTCGGTTAGGACGCTTATTTCGAGGCATGCGATCCT
GGAGTTATTGGGGCCTCTGCATTGGCGTTGGCTTATGTGTGTTTGCTCCGTTCGTGATACCGTTTAGCGATCGCTTAGAA
AATACCCTGTACCGAGCCCCCATTGTTAGTCGTATTCAGTCCACCTACCAGCGCATCGTCCTCACCCGCTCTGGCAACGA
CACCCGGCTCTATCTCGATGGCGACCTCCAGCTATCCACCGTCGATGAATACCGCTATCACGAAGCCCTGGTCCATCCAG
CCCTGAGCACCCATCCCCAGCGACGCCAAGTGCTGGTGCTCGGAGCTGGAGACGGCATGGCCGTAAGGGAAATCCTTAAA
TGGCCGGAAGTTGAAAAAGTGGTGCTTGTTGAGCTGGATCCTGCCGTCGTGAAACTCGCCCGTCAGTATCCTGTGCTCGT
CGATCGCAACGCCCATGCCCTAGACGATCCTCGGGTAGCCGTGGTGTATGGAGATGCCTTTAAGCTCGCCCCTCAGCTGC
CGGATACCTTTGACGTCATCATTGCCGATTTCCCAGATCCCGATCGCCCTGCCCTCGCCAAGCTCTACTCCCAAGGATTC
TATCGACAACTGTTCGCTCGCCTTGCCCCCAACGGCATCTTCGTCACCCAGGCATCCAGTCCTTTTTTCGCCCCAAAGGT
TATGGACTGTATCGCCACAACCCTAACAGCTACTGATTTACAGGTTTATCCCTACACCGTCAATGTGCCGAGCTTTGGTC
CCTGGGGGTTTGTCTTGGCGTCGCCGAAGCCAGTCCAGTTTTCATCCCAAAGCCTAACGGTTCCCACTCGGTTCCTGACC
CCAGCCCTCCTCCCTGAACTCTTTCAACTTCCCAAAGATATTTCTATCGGTAACGCCAAGATCAATCGGCTCACCGATCC
AGTTATAGTTCAGTACCAAGCCGATCCACGCTGGTCGGCCTATTATTGA

Upstream 100 bases:

>100_bases
CTGGGCGCTATCGCGGTGGTGTTTGGATCGTCTCTCCGGCCCGGTCTTCCTACAGCGGATTCCGCGGTGGCAGTCTGGGT
GTAGGTAAATAAGTTGCGGT

Downstream 100 bases:

>100_bases
AACACTCTAGAGGCAGACTATGGCTTACCTTTGGCTATTTATCATCATCATGGGCGTATTGGGTGTTGTCATCTATATGA
GACAACAGTCTGGTAAATCC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MAESESTSGGVQRPESIPLSSSQQRWLLAAAAISSSVGLAAELLLGTLASYLVGNTALAYGVAVGGFLAAMGLGAYISQF
IAVDNQQSQLIKVFLQIELWVAPLTAGLPIGLFVVFVADGPIWLGLFLVTLILGILSGLEVPILTRIIEQDRDVKFALAG
VLALDYVGALIGSLAFPVLLLPLLGLFPTAVLIGSLPAFMVFRLGRLFRGMRSWSYWGLCIGVGLCVFAPFVIPFSDRLE
NTLYRAPIVSRIQSTYQRIVLTRSGNDTRLYLDGDLQLSTVDEYRYHEALVHPALSTHPQRRQVLVLGAGDGMAVREILK
WPEVEKVVLVELDPAVVKLARQYPVLVDRNAHALDDPRVAVVYGDAFKLAPQLPDTFDVIIADFPDPDRPALAKLYSQGF
YRQLFARLAPNGIFVTQASSPFFAPKVMDCIATTLTATDLQVYPYTVNVPSFGPWGFVLASPKPVQFSSQSLTVPTRFLT
PALLPELFQLPKDISIGNAKINRLTDPVIVQYQADPRWSAYY

Sequences:

>Translated_522_residues
MAESESTSGGVQRPESIPLSSSQQRWLLAAAAISSSVGLAAELLLGTLASYLVGNTALAYGVAVGGFLAAMGLGAYISQF
IAVDNQQSQLIKVFLQIELWVAPLTAGLPIGLFVVFVADGPIWLGLFLVTLILGILSGLEVPILTRIIEQDRDVKFALAG
VLALDYVGALIGSLAFPVLLLPLLGLFPTAVLIGSLPAFMVFRLGRLFRGMRSWSYWGLCIGVGLCVFAPFVIPFSDRLE
NTLYRAPIVSRIQSTYQRIVLTRSGNDTRLYLDGDLQLSTVDEYRYHEALVHPALSTHPQRRQVLVLGAGDGMAVREILK
WPEVEKVVLVELDPAVVKLARQYPVLVDRNAHALDDPRVAVVYGDAFKLAPQLPDTFDVIIADFPDPDRPALAKLYSQGF
YRQLFARLAPNGIFVTQASSPFFAPKVMDCIATTLTATDLQVYPYTVNVPSFGPWGFVLASPKPVQFSSQSLTVPTRFLT
PALLPELFQLPKDISIGNAKINRLTDPVIVQYQADPRWSAYY
>Mature_521_residues
AESESTSGGVQRPESIPLSSSQQRWLLAAAAISSSVGLAAELLLGTLASYLVGNTALAYGVAVGGFLAAMGLGAYISQFI
AVDNQQSQLIKVFLQIELWVAPLTAGLPIGLFVVFVADGPIWLGLFLVTLILGILSGLEVPILTRIIEQDRDVKFALAGV
LALDYVGALIGSLAFPVLLLPLLGLFPTAVLIGSLPAFMVFRLGRLFRGMRSWSYWGLCIGVGLCVFAPFVIPFSDRLEN
TLYRAPIVSRIQSTYQRIVLTRSGNDTRLYLDGDLQLSTVDEYRYHEALVHPALSTHPQRRQVLVLGAGDGMAVREILKW
PEVEKVVLVELDPAVVKLARQYPVLVDRNAHALDDPRVAVVYGDAFKLAPQLPDTFDVIIADFPDPDRPALAKLYSQGFY
RQLFARLAPNGIFVTQASSPFFAPKVMDCIATTLTATDLQVYPYTVNVPSFGPWGFVLASPKPVQFSSQSLTVPTRFLTP
ALLPELFQLPKDISIGNAKINRLTDPVIVQYQADPRWSAYY

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:
GO:0003824: Spermidine synthase, putative
GO:0004766: Spermidine synthase, putative
GO:0008295: Spermidine synthase, putative

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=222, Percent_Identity=31.5315315315315, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786312, Length=266, Percent_Identity=30.4511278195489, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI212645616, Length=222, Percent_Identity=33.7837837837838, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25154106, Length=238, Percent_Identity=30.672268907563, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325326, Length=255, Percent_Identity=30.5882352941176, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323175, Length=268, Percent_Identity=30.2238805970149, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24645443, Length=265, Percent_Identity=29.811320754717, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21355941, Length=211, Percent_Identity=30.8056872037915, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 56983; Mature: 56852

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESESTSGGVQRPESIPLSSSQQRWLLAAAAISSSVGLAAELLLGTLASYLVGNTALAY
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
GVAVGGFLAAMGLGAYISQFIAVDNQQSQLIKVFLQIELWVAPLTAGLPIGLFVVFVADG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECC
PIWLGLFLVTLILGILSGLEVPILTRIIEQDRDVKFALAGVLALDYVGALIGSLAFPVLL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPLLGLFPTAVLIGSLPAFMVFRLGRLFRGMRSWSYWGLCIGVGLCVFAPFVIPFSDRLE
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
NTLYRAPIVSRIQSTYQRIVLTRSGNDTRLYLDGDLQLSTVDEYRYHEALVHPALSTHPQ
HHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHCCCCC
RRQVLVLGAGDGMAVREILKWPEVEKVVLVELDPAVVKLARQYPVLVDRNAHALDDPRVA
CCEEEEEECCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCEEEECCCCCCCCCCEE
VVYGDAFKLAPQLPDTFDVIIADFPDPDRPALAKLYSQGFYRQLFARLAPNGIFVTQASS
EEECCHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PFFAPKVMDCIATTLTATDLQVYPYTVNVPSFGPWGFVLASPKPVQFSSQSLTVPTRFLT
CCCCHHHHHHHHHHHEECCEEEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCHHHHC
PALLPELFQLPKDISIGNAKINRLTDPVIVQYQADPRWSAYY
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
AESESTSGGVQRPESIPLSSSQQRWLLAAAAISSSVGLAAELLLGTLASYLVGNTALAY
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
GVAVGGFLAAMGLGAYISQFIAVDNQQSQLIKVFLQIELWVAPLTAGLPIGLFVVFVADG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECC
PIWLGLFLVTLILGILSGLEVPILTRIIEQDRDVKFALAGVLALDYVGALIGSLAFPVLL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPLLGLFPTAVLIGSLPAFMVFRLGRLFRGMRSWSYWGLCIGVGLCVFAPFVIPFSDRLE
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
NTLYRAPIVSRIQSTYQRIVLTRSGNDTRLYLDGDLQLSTVDEYRYHEALVHPALSTHPQ
HHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHCCCCC
RRQVLVLGAGDGMAVREILKWPEVEKVVLVELDPAVVKLARQYPVLVDRNAHALDDPRVA
CCEEEEEECCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCEEEECCCCCCCCCCEE
VVYGDAFKLAPQLPDTFDVIIADFPDPDRPALAKLYSQGFYRQLFARLAPNGIFVTQASS
EEECCHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PFFAPKVMDCIATTLTATDLQVYPYTVNVPSFGPWGFVLASPKPVQFSSQSLTVPTRFLT
CCCCHHHHHHHHHHHEECCEEEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCHHHHC
PALLPELFQLPKDISIGNAKINRLTDPVIVQYQADPRWSAYY
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]