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The map label for this gene is rimO

Identifier: 158334864

GI number: 158334864

Start: 1680368

End: 1681699

Strand: Direct

Name: rimO

Synonym: AM1_1701

Alternate gene names: 158334864

Gene position: 1680368-1681699 (Clockwise)

Preceding gene: 158334863

Following gene: 158334865

Centisome position: 25.84

GC content: 52.03

Gene sequence:

>1332_bases
ATGGCCAATCAGCCCTCCGTCGCCGTTGCTCATCTCGGCTGTGAAAAAAATCGTGTTGACACTGAACATATGCTGGGATT
GCTGGTGGAAGCAGGCTATCCCGTCGACAGCGATGAAGCCTTTGCCGACTACGTGATCGTTAACACCTGCAGCTTTATCC
AAGCAGCCCGGGAAGAGTCTGTACGCACCCTAGTCGAACTAGCCGAAGCCAACAAAAAAATTGTGATTACAGGCTGCATG
GCTCAGCATTTCCAAGAGGAATTGCTAGAAGAACTCCCCGAAGCCGTTGCCCTCGTCGGTACCGGAGACTATCACAAAAT
CGTAGATGTGATTCAGCGCGCCGAAGCGGGAGAGCGGGTGAAAGAAGTCTCACCTGAACCGACCTATATCGCCGATGAAA
CCGTTCCTCGGTATCGCACCACCACCGAAGGCACTGCCTATGTCCGCATTGCTGAAGGCTGTGACTATGGCTGTGCCTTT
TGCATCATTCCCCATTTGCGTGGCAAACAGCGGTCGCGCTCGATCGAGTCCATCGTGGCGGAAGCCCAACAATTAGCCGC
CGAAGGCGTGCAGGAGCTAATTTTAATTTCCCAAATTACCACTAACTACGGCATTGACCTCTACGGCAAACCCCAGCTCG
CTGAACTACTTCGGGCTCTAGGCGAAGTTGATGTCCCTTGGATTCGCATGCATTACGCCTATCCCACGGGCTTAACCCCA
GAAGTAATGGCGGCCATTCAAGAAACGGATAACGTTCTGCCCTACTTAGATTTGCCTTTGCAACATTCCCACCCAGAAGT
CTTGCGGGCTATGAATCGACCTTGGCAAGGTCAAGTGAACGATCAAATTATTGAGAAAATCAAAACTGCCTTGCCGGATG
CGGTTCTCCGCACCACATTTATTGTGGGATTCCCCGGAGAAACAGACGAGCATTTTGAGCATCTCTGTGAGTTTGTCCAG
CGGCATGAATTCGATCACGTAGGCGTGTTTACCTTCTCGCCAGAAGAAGGCACCCCCGCGTTTGATTTGCCCAATCAATT
GCCTCAAGACGTGATGGATGCACGACGCGATCGCTTGATGGCATTGCAACAACCCATCTCCTGGCAACAAAACCAGCAAG
AAGTTGGCAAAACGGTACAAGTCTTAATCGAGCAAGAACATCCTGGCTCTGGTCAGCTCATCGGTCGCTCGCCTCGGTTC
TCTGCCGATGTGGACGGTCTCGTCTATATCGATCCTGGTGAGGCTCCATCACCTCGTTTAGGCAGCTTAACCCCTGTCCA
GATTACAGATGCAGATGCCTACGACCTCCAGGGACAACTGGTGAGCCAATAA

Upstream 100 bases:

>100_bases
ATCCTGCAAGACTCGGAGGCCATCTTCGCCCAAGCTGACTGAATTTCAGATCATGAGATAGGATCACGAAGTATTCTTTC
TCAAAATTGATCTACTCTGC

Downstream 100 bases:

>100_bases
TCATTGCCCTTGGCATTGGAATGTTTTTTTAGACAGTTGGAGTCGCTCATCTGTCTAACCAAATGTCATGATTACTTGAC
TTATAATGCATTTTGTTACA

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MANQPSVAVAHLGCEKNRVDTEHMLGLLVEAGYPVDSDEAFADYVIVNTCSFIQAAREESVRTLVELAEANKKIVITGCM
AQHFQEELLEELPEAVALVGTGDYHKIVDVIQRAEAGERVKEVSPEPTYIADETVPRYRTTTEGTAYVRIAEGCDYGCAF
CIIPHLRGKQRSRSIESIVAEAQQLAAEGVQELILISQITTNYGIDLYGKPQLAELLRALGEVDVPWIRMHYAYPTGLTP
EVMAAIQETDNVLPYLDLPLQHSHPEVLRAMNRPWQGQVNDQIIEKIKTALPDAVLRTTFIVGFPGETDEHFEHLCEFVQ
RHEFDHVGVFTFSPEEGTPAFDLPNQLPQDVMDARRDRLMALQQPISWQQNQQEVGKTVQVLIEQEHPGSGQLIGRSPRF
SADVDGLVYIDPGEAPSPRLGSLTPVQITDADAYDLQGQLVSQ

Sequences:

>Translated_443_residues
MANQPSVAVAHLGCEKNRVDTEHMLGLLVEAGYPVDSDEAFADYVIVNTCSFIQAAREESVRTLVELAEANKKIVITGCM
AQHFQEELLEELPEAVALVGTGDYHKIVDVIQRAEAGERVKEVSPEPTYIADETVPRYRTTTEGTAYVRIAEGCDYGCAF
CIIPHLRGKQRSRSIESIVAEAQQLAAEGVQELILISQITTNYGIDLYGKPQLAELLRALGEVDVPWIRMHYAYPTGLTP
EVMAAIQETDNVLPYLDLPLQHSHPEVLRAMNRPWQGQVNDQIIEKIKTALPDAVLRTTFIVGFPGETDEHFEHLCEFVQ
RHEFDHVGVFTFSPEEGTPAFDLPNQLPQDVMDARRDRLMALQQPISWQQNQQEVGKTVQVLIEQEHPGSGQLIGRSPRF
SADVDGLVYIDPGEAPSPRLGSLTPVQITDADAYDLQGQLVSQ
>Mature_442_residues
ANQPSVAVAHLGCEKNRVDTEHMLGLLVEAGYPVDSDEAFADYVIVNTCSFIQAAREESVRTLVELAEANKKIVITGCMA
QHFQEELLEELPEAVALVGTGDYHKIVDVIQRAEAGERVKEVSPEPTYIADETVPRYRTTTEGTAYVRIAEGCDYGCAFC
IIPHLRGKQRSRSIESIVAEAQQLAAEGVQELILISQITTNYGIDLYGKPQLAELLRALGEVDVPWIRMHYAYPTGLTPE
VMAAIQETDNVLPYLDLPLQHSHPEVLRAMNRPWQGQVNDQIIEKIKTALPDAVLRTTFIVGFPGETDEHFEHLCEFVQR
HEFDHVGVFTFSPEEGTPAFDLPNQLPQDVMDARRDRLMALQQPISWQQNQQEVGKTVQVLIEQEHPGSGQLIGRSPRFS
ADVDGLVYIDPGEAPSPRLGSLTPVQITDADAYDLQGQLVSQ

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=496, Percent_Identity=26.8145161290323, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI28872782, Length=496, Percent_Identity=26.8145161290323, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI93277076, Length=446, Percent_Identity=26.9058295964126, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1787057, Length=457, Percent_Identity=36.7614879649891, Blast_Score=281, Evalue=7e-77,
Organism=Escherichia coli, GI1786882, Length=463, Percent_Identity=29.8056155507559, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=482, Percent_Identity=26.5560165975104, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71996771, Length=366, Percent_Identity=25.6830601092896, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356207, Length=470, Percent_Identity=27.0212765957447, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI19922432, Length=455, Percent_Identity=26.3736263736264, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_ACAM1 (B0CB83)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516036.1
- ProteinModelPortal:   B0CB83
- SMR:   B0CB83
- GeneID:   5680517
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1701
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK893584
- BioCyc:   AMAR329726:AM1_1701-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49235; Mature: 49104

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQPSVAVAHLGCEKNRVDTEHMLGLLVEAGYPVDSDEAFADYVIVNTCSFIQAAREES
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
VRTLVELAEANKKIVITGCMAQHFQEELLEELPEAVALVGTGDYHKIVDVIQRAEAGERV
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHH
KEVSPEPTYIADETVPRYRTTTEGTAYVRIAEGCDYGCAFCIIPHLRGKQRSRSIESIVA
HHCCCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHH
EAQQLAAEGVQELILISQITTNYGIDLYGKPQLAELLRALGEVDVPWIRMHYAYPTGLTP
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCH
EVMAAIQETDNVLPYLDLPLQHSHPEVLRAMNRPWQGQVNDQIIEKIKTALPDAVLRTTF
HHHHHHHHHCCCCCEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHEE
IVGFPGETDEHFEHLCEFVQRHEFDHVGVFTFSPEEGTPAFDLPNQLPQDVMDARRDRLM
EEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALQQPISWQQNQQEVGKTVQVLIEQEHPGSGQLIGRSPRFSADVDGLVYIDPGEAPSPRL
HHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCEEEECCCCCCCCCC
GSLTPVQITDADAYDLQGQLVSQ
CCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
ANQPSVAVAHLGCEKNRVDTEHMLGLLVEAGYPVDSDEAFADYVIVNTCSFIQAAREES
CCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
VRTLVELAEANKKIVITGCMAQHFQEELLEELPEAVALVGTGDYHKIVDVIQRAEAGERV
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHH
KEVSPEPTYIADETVPRYRTTTEGTAYVRIAEGCDYGCAFCIIPHLRGKQRSRSIESIVA
HHCCCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHH
EAQQLAAEGVQELILISQITTNYGIDLYGKPQLAELLRALGEVDVPWIRMHYAYPTGLTP
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCH
EVMAAIQETDNVLPYLDLPLQHSHPEVLRAMNRPWQGQVNDQIIEKIKTALPDAVLRTTF
HHHHHHHHHCCCCCEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHEE
IVGFPGETDEHFEHLCEFVQRHEFDHVGVFTFSPEEGTPAFDLPNQLPQDVMDARRDRLM
EEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALQQPISWQQNQQEVGKTVQVLIEQEHPGSGQLIGRSPRFSADVDGLVYIDPGEAPSPRL
HHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCEEEECCCCCCCCCC
GSLTPVQITDADAYDLQGQLVSQ
CCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA