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The map label for this gene is glpK

Identifier: 158334851

GI number: 158334851

Start: 1663836

End: 1665344

Strand: Direct

Name: glpK

Synonym: AM1_1688

Alternate gene names: 158334851

Gene position: 1663836-1665344 (Clockwise)

Preceding gene: 158334850

Following gene: 158334852

Centisome position: 25.58

GC content: 52.75

Gene sequence:

>1509_bases
ATGACTTCAGCTACCTACATCATGGCCTTGGATTTGGGGACCACTGGCAATCGTGCCATTCTCTTCGACCAAGACGGCCA
GATTGTCGATCAAGCCTATAAAGAACTTCCTCAGTATTATCCTCAGCCGGGATGGGTGGAACATGATGCCTTAGAAATCT
GGCGCGATACCCGTTGGGCCATGGAAACGGTGGTCACCCAAGCCCACATTGATCCCGCTCAGATCGCAGCCATCGGCCTC
ACTGTGCAGCGAGAAACCTGCCTGCTCTGGGATAAAACCACGGGCCAGCCCTTGCACAAAGCCATTGTTTGGCAAGACCG
ACGCACGGCAGCCTACTGTAACCAACTGGCTGAGCAAGGCCATACCCAGGATATTTATGATCGCACTGGTTTAGTCCTGG
ATGCCTATTTTTCCGGGAGCAAGTTAGCCTGGCTGCTTACAGAAGTGAAGAGGCAAAACCCCAATCTCAATCTAGACAAT
GTTATTGCTGGCACCATCGATACCTGGGCCTTGTGGAATCTTACAGGCGGCAAGGTTCACGCCACGGATCACAGTAATGC
CAGTCGGACCCTACTGCTGAATTTAAGCCAAGGCACCTGGGATGACCATCTTCTCGATCTATTTGGCATTCCCAAGTCAT
TTATGCCCGCCATTCAGCCCAGCTTAGGTATCTTTGGCAAAACCGATACCAAGTTCCTGGGTCAAGAAATTCCCATTGCC
GCCATCTTTGGGGACCAGCAGGCCGCCTTATTTGCCCATGGCTGCGATCGCCCTGGTGCCCTCAAATGCACCTATGGCAC
CGGGTCTTTTCTAGTCGCCCATACGGGGAGTAACATTGCTCGCTCCAAGAACCGTCTGCTGTCCACCGTGGCTTGGACCC
AAACCGATAAGGGACAGACCCAAACTGGCTATGCCATCGAAGGCAGTATGTTTACCTCCGGGGCTTGTATCCAGTGGCTG
CGAGATGGCCTTAAGCTGATTGCCAATGCTGCTGAAACAGAAGGATTAGCCCAAGCAGTCGAAGATAACGGTGGCGTTTA
TTTTGTCCCTGCCCTAAGCGGTCTCGGTGCTCCCCACTGGGATATGAGCGCTCGGGGGGCCTTTTTGGGAATTACGCGCG
GGGCTCAACGAGAGCACATGGTACGGGCTGTCTTAGAAGCGATCGCTTTCCAAACCAAGGAAGTGGTTGATGCGGTCAAT
CAAGATTGTGGCTCTCCTATTCAGCAACTCAAAGTCGATGGTGGCGCTTGCCAGAACAATTTCTTGATGCAGTACCAAGC
TGATGTTTTGGGCATTCCGGTTGAACGTCCTGCGGTTTTGGATGCCACTGCCCAAGGAGCAGCGTTCGGGGCAGGGTTAG
CCATAGGGGTGTGGAAAGACTATGCAGGGTTAGTCGCTGCTCGCAAAATCGATCAAGTCTTTAAACCAGGTGCGAATGCC
CAAACCGCTCAAGCCAATTTTAAAACCTGGCAAAAAGCAGTGGAACGCGCTAAAAATTGGGCCGACTAG

Upstream 100 bases:

>100_bases
TAGCAATCGGGAATGATGTCGAAAGCTTAAGATCTTTTGAACTAAAGGACACACAAGGGGTACTCCTAGAGAAGATTGTG
TAAACCCTTCGAGGAAGATC

Downstream 100 bases:

>100_bases
TATCAATCGCGACATTTTTTCTCGTCTGTAACTCGTTAACATAAGGGTCGTCCAAATTTCCCCAACGAGAATCAAAATCG
CCATGGTAGAACTATTAAAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTSATYIMALDLGTTGNRAILFDQDGQIVDQAYKELPQYYPQPGWVEHDALEIWRDTRWAMETVVTQAHIDPAQIAAIGL
TVQRETCLLWDKTTGQPLHKAIVWQDRRTAAYCNQLAEQGHTQDIYDRTGLVLDAYFSGSKLAWLLTEVKRQNPNLNLDN
VIAGTIDTWALWNLTGGKVHATDHSNASRTLLLNLSQGTWDDHLLDLFGIPKSFMPAIQPSLGIFGKTDTKFLGQEIPIA
AIFGDQQAALFAHGCDRPGALKCTYGTGSFLVAHTGSNIARSKNRLLSTVAWTQTDKGQTQTGYAIEGSMFTSGACIQWL
RDGLKLIANAAETEGLAQAVEDNGGVYFVPALSGLGAPHWDMSARGAFLGITRGAQREHMVRAVLEAIAFQTKEVVDAVN
QDCGSPIQQLKVDGGACQNNFLMQYQADVLGIPVERPAVLDATAQGAAFGAGLAIGVWKDYAGLVAARKIDQVFKPGANA
QTAQANFKTWQKAVERAKNWAD

Sequences:

>Translated_502_residues
MTSATYIMALDLGTTGNRAILFDQDGQIVDQAYKELPQYYPQPGWVEHDALEIWRDTRWAMETVVTQAHIDPAQIAAIGL
TVQRETCLLWDKTTGQPLHKAIVWQDRRTAAYCNQLAEQGHTQDIYDRTGLVLDAYFSGSKLAWLLTEVKRQNPNLNLDN
VIAGTIDTWALWNLTGGKVHATDHSNASRTLLLNLSQGTWDDHLLDLFGIPKSFMPAIQPSLGIFGKTDTKFLGQEIPIA
AIFGDQQAALFAHGCDRPGALKCTYGTGSFLVAHTGSNIARSKNRLLSTVAWTQTDKGQTQTGYAIEGSMFTSGACIQWL
RDGLKLIANAAETEGLAQAVEDNGGVYFVPALSGLGAPHWDMSARGAFLGITRGAQREHMVRAVLEAIAFQTKEVVDAVN
QDCGSPIQQLKVDGGACQNNFLMQYQADVLGIPVERPAVLDATAQGAAFGAGLAIGVWKDYAGLVAARKIDQVFKPGANA
QTAQANFKTWQKAVERAKNWAD
>Mature_501_residues
TSATYIMALDLGTTGNRAILFDQDGQIVDQAYKELPQYYPQPGWVEHDALEIWRDTRWAMETVVTQAHIDPAQIAAIGLT
VQRETCLLWDKTTGQPLHKAIVWQDRRTAAYCNQLAEQGHTQDIYDRTGLVLDAYFSGSKLAWLLTEVKRQNPNLNLDNV
IAGTIDTWALWNLTGGKVHATDHSNASRTLLLNLSQGTWDDHLLDLFGIPKSFMPAIQPSLGIFGKTDTKFLGQEIPIAA
IFGDQQAALFAHGCDRPGALKCTYGTGSFLVAHTGSNIARSKNRLLSTVAWTQTDKGQTQTGYAIEGSMFTSGACIQWLR
DGLKLIANAAETEGLAQAVEDNGGVYFVPALSGLGAPHWDMSARGAFLGITRGAQREHMVRAVLEAIAFQTKEVVDAVNQ
DCGSPIQQLKVDGGACQNNFLMQYQADVLGIPVERPAVLDATAQGAAFGAGLAIGVWKDYAGLVAARKIDQVFKPGANAQ
TAQANFKTWQKAVERAKNWAD

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:
GO:0000166: Glycerol kinase
GO:0004370: Glycerol kinase
GO:0005524: Glycerol kinase
GO:0005975: Glycerol kinase
GO:0006071: Glycerol kinase
GO:0006072: Glycerol kinase
GO:0016301: Glycerol kinase
GO:0016310: Glycerol kinase
GO:0016740: Glycerol kinase
GO:0016773: Glycerol kinase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI4504007, Length=505, Percent_Identity=40.5940594059406, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI189409120, Length=505, Percent_Identity=40.5940594059406, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI42794763, Length=510, Percent_Identity=40, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI41393575, Length=501, Percent_Identity=38.9221556886228, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI88196792, Length=513, Percent_Identity=32.943469785575, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1790361, Length=503, Percent_Identity=46.1232604373757, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1790005, Length=464, Percent_Identity=24.3534482758621, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1789987, Length=494, Percent_Identity=25.3036437246964, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1787789, Length=489, Percent_Identity=26.3803680981595, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=36.5079365079365, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI71986140, Length=532, Percent_Identity=27.4436090225564, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71986147, Length=303, Percent_Identity=32.013201320132, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321755, Length=547, Percent_Identity=31.9926873857404, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI17864214, Length=512, Percent_Identity=37.890625, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24654573, Length=435, Percent_Identity=40.4597701149425, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24655555, Length=476, Percent_Identity=36.9747899159664, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24655560, Length=476, Percent_Identity=36.9747899159664, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24655570, Length=476, Percent_Identity=36.9747899159664, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24655574, Length=476, Percent_Identity=36.9747899159664, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24655565, Length=476, Percent_Identity=36.9747899159664, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI19922058, Length=531, Percent_Identity=31.0734463276836, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24652871, Length=496, Percent_Identity=31.8548387096774, Blast_Score=235, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=30.0387596899225, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=29.6786389413989, Blast_Score=232, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24656372, Length=350, Percent_Identity=32.2857142857143, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI45552909, Length=288, Percent_Identity=30.5555555555556, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_ACAM1 (B0CB70)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516023.1
- ProteinModelPortal:   B0CB70
- GeneID:   5680504
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1688
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   CLSK893231
- BioCyc:   AMAR329726:AM1_1688-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54636; Mature: 54505

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 14-14 BINDING 18-18 BINDING 84-84 BINDING 136-136 BINDING 245-245 BINDING 267-267 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSATYIMALDLGTTGNRAILFDQDGQIVDQAYKELPQYYPQPGWVEHDALEIWRDTRWA
CCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
METVVTQAHIDPAQIAAIGLTVQRETCLLWDKTTGQPLHKAIVWQDRRTAAYCNQLAEQG
HHHHHHHCCCCHHHEEEEEEEEECCEEEEEECCCCCHHHHHHEECCCHHHHHHHHHHHCC
HTQDIYDRTGLVLDAYFSGSKLAWLLTEVKRQNPNLNLDNVIAGTIDTWALWNLTGGKVH
CCCHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHEEECCCCCEEEEECCCCEEE
ATDHSNASRTLLLNLSQGTWDDHLLDLFGIPKSFMPAIQPSLGIFGKTDTKFLGQEIPIA
ECCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHCCCCCCEE
AIFGDQQAALFAHGCDRPGALKCTYGTGSFLVAHTGSNIARSKNRLLSTVAWTQTDKGQT
EEECCCCCEEEECCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHEECCCCCCCC
QTGYAIEGSMFTSGACIQWLRDGLKLIANAAETEGLAQAVEDNGGVYFVPALSGLGAPHW
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
DMSARGAFLGITRGAQREHMVRAVLEAIAFQTKEVVDAVNQDCGSPIQQLKVDGGACQNN
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCC
FLMQYQADVLGIPVERPAVLDATAQGAAFGAGLAIGVWKDYAGLVAARKIDQVFKPGANA
EEEEEECCEEECCCCCCCEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCC
QTAQANFKTWQKAVERAKNWAD
CCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSATYIMALDLGTTGNRAILFDQDGQIVDQAYKELPQYYPQPGWVEHDALEIWRDTRWA
CCCEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
METVVTQAHIDPAQIAAIGLTVQRETCLLWDKTTGQPLHKAIVWQDRRTAAYCNQLAEQG
HHHHHHHCCCCHHHEEEEEEEEECCEEEEEECCCCCHHHHHHEECCCHHHHHHHHHHHCC
HTQDIYDRTGLVLDAYFSGSKLAWLLTEVKRQNPNLNLDNVIAGTIDTWALWNLTGGKVH
CCCHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHEEECCCCCEEEEECCCCEEE
ATDHSNASRTLLLNLSQGTWDDHLLDLFGIPKSFMPAIQPSLGIFGKTDTKFLGQEIPIA
ECCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHCCCCCCEE
AIFGDQQAALFAHGCDRPGALKCTYGTGSFLVAHTGSNIARSKNRLLSTVAWTQTDKGQT
EEECCCCCEEEECCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHEECCCCCCCC
QTGYAIEGSMFTSGACIQWLRDGLKLIANAAETEGLAQAVEDNGGVYFVPALSGLGAPHW
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
DMSARGAFLGITRGAQREHMVRAVLEAIAFQTKEVVDAVNQDCGSPIQQLKVDGGACQNN
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCC
FLMQYQADVLGIPVERPAVLDATAQGAAFGAGLAIGVWKDYAGLVAARKIDQVFKPGANA
EEEEEECCEEECCCCCCCEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCC
QTAQANFKTWQKAVERAKNWAD
CCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA