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The map label for this gene is aprX [H]

Identifier: 158334833

GI number: 158334833

Start: 1649058

End: 1650737

Strand: Direct

Name: aprX [H]

Synonym: AM1_1670

Alternate gene names: 158334833

Gene position: 1649058-1650737 (Clockwise)

Preceding gene: 158334832

Following gene: 158334840

Centisome position: 25.36

GC content: 45.54

Gene sequence:

>1680_bases
TTGAGTGATGCTGAAACACCAACATTACCTCAGGATATTGTGGCGGTCAGTATTAAGGTTAGTGATAAAACAGCCTTTGA
TGCTAGTTACCCAACATTAGCGCCAGAAGAACAGCTTGTTCCTATTGGGGATGAATTCTACTCAGGTAAATTAAGTGTCT
CCCACCTCCAAGGATTACTTAGTCATGCGAATGTAGAGCGCATCGAAACCAAAAAGACTCATAGTCTGACTTTGGATAGA
GCCTCTACGGATATTGAGCTGTTAACAGCGGGAGGAAGTCGATCTATTCCCCAGACAGGAAAAGATGTTTTGGTGGGAAT
CGTAGATTCGGGATTCGATCTGTCTCATCCTATGTTCCGAGACGTTAGTGGTAATCTCCGAGTCGTGGCCTTATTGGATC
AGACCAATGGTAATCAAGAATACAATACAAGCCAGTTGCAAACGCAATGGGGGGCCGGTGGCTCTAGACCTGGCGAAGAT
AAGGATGGCCATGGTACTCATGTTGCATCTATCGCTGGAGGGAGTAAGTTAGACCAATATGAGGGAATCGCGCCTGATGC
TAAATTCTTGCTGGTTAAAACAGATTTTACCAATGTGGATTTAGCCGTATCTTGGATTTACGATCAAGCAGATGGCAATC
CATGTGTCGTCAATCTCAGCCTTGGGTATCATTTCGGCGCACATGATGGAACGAGTAATGAAGAACGTTTGTACGATACC
TTGGTCGGTCCTGGAAAAATCTTGGTTGTGTCCGCCGGGAATGAGCGGAATGACTCGATTCATCTGAATGGCATGTTTGT
CCCCAACCAATTGGAAGAAGTTGTCTTTGATATTTTGCCTCGCAATTCCGCCGGCGTTCCTCCCTTTGTGGCTACGACTT
TCTGGTATCCGGATAATGATGACTACGAACTAGATTTAATCACACCATCTGGTCAGGCACTTCCAACCCCGACTCTGGGT
AATACGGATAGCTATAGCGCTGGAAATCTGAGTATAGAGATTAGCCGAACAGCTTACACCATCAGCGATCTAACACAAAT
TCAGATCTCTATTAGCTTTAGTAACTTTAGTATTCCTCCTGGTAGCTTGCAGAATTGGCGTTTTCGTATTCGTTGTGTGT
CGGCAACTACAGGCAGATTTGATGCCTGGTTTCATAATTCAGGCTTTGCACGATTCCGATCTCATCCTTTAGTACAGGCT
CAAGGGACTATTGGTATCGCCGCAACTGCCCGTGGTTGTATCGCTGTTGCTAGCCATGTTTCCAAGAATAGTTGGGATAG
TGATGATGGTCCGCAACAAGATCTTATGGCAGTGATGGGTCGCTCTAGTAGTTTTAGTAGTCTGGGTCCCACTCGCGATG
GACGATTGAAACCTGAAATTTCTGCTCCGGGCCAATATATTACTGCGGCACTGGCTGAGAACTCAGACTTATCAGATGTT
AGTGTCCGAGCTGATGAGACCAATAGTTTGCTGACGATTGAAGGCACAAGCATGTCAGCTCCAGTTGTGACTGGAGCAAT
TGCATTGATGCTGCAAAAAAATCCTAGTCTAACGCCGGAACAAGTGAAAGAGATTTTGAAAGAGTCTGCTAAAAAAGATG
CACATACGCAAGATACAGCTTGGACGCCTGCCTACGGTTATGGAAAGTTGGACATTGCCGCAGCAGTAAGCAGAGTTTAA

Upstream 100 bases:

>100_bases
TTTTTCGATTACTAATAAAGCTTCTGTCTCTTTATCTTCAATTTTAGATAGTCAGCGTCAACATCAAAGACCTACACCAG
CGGCCAGATCGACTGGCTTT

Downstream 100 bases:

>100_bases
TCCCAACATAATCTCCCCAAGAGAATTTATTTAACATCTTTTTGGGGAGCTTGATACATTTGATGAGCTTGCTTGATCTC
GACTATCGCGAGATCTTCTA

Product: subtilase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MSDAETPTLPQDIVAVSIKVSDKTAFDASYPTLAPEEQLVPIGDEFYSGKLSVSHLQGLLSHANVERIETKKTHSLTLDR
ASTDIELLTAGGSRSIPQTGKDVLVGIVDSGFDLSHPMFRDVSGNLRVVALLDQTNGNQEYNTSQLQTQWGAGGSRPGED
KDGHGTHVASIAGGSKLDQYEGIAPDAKFLLVKTDFTNVDLAVSWIYDQADGNPCVVNLSLGYHFGAHDGTSNEERLYDT
LVGPGKILVVSAGNERNDSIHLNGMFVPNQLEEVVFDILPRNSAGVPPFVATTFWYPDNDDYELDLITPSGQALPTPTLG
NTDSYSAGNLSIEISRTAYTISDLTQIQISISFSNFSIPPGSLQNWRFRIRCVSATTGRFDAWFHNSGFARFRSHPLVQA
QGTIGIAATARGCIAVASHVSKNSWDSDDGPQQDLMAVMGRSSSFSSLGPTRDGRLKPEISAPGQYITAALAENSDLSDV
SVRADETNSLLTIEGTSMSAPVVTGAIALMLQKNPSLTPEQVKEILKESAKKDAHTQDTAWTPAYGYGKLDIAAAVSRV

Sequences:

>Translated_559_residues
MSDAETPTLPQDIVAVSIKVSDKTAFDASYPTLAPEEQLVPIGDEFYSGKLSVSHLQGLLSHANVERIETKKTHSLTLDR
ASTDIELLTAGGSRSIPQTGKDVLVGIVDSGFDLSHPMFRDVSGNLRVVALLDQTNGNQEYNTSQLQTQWGAGGSRPGED
KDGHGTHVASIAGGSKLDQYEGIAPDAKFLLVKTDFTNVDLAVSWIYDQADGNPCVVNLSLGYHFGAHDGTSNEERLYDT
LVGPGKILVVSAGNERNDSIHLNGMFVPNQLEEVVFDILPRNSAGVPPFVATTFWYPDNDDYELDLITPSGQALPTPTLG
NTDSYSAGNLSIEISRTAYTISDLTQIQISISFSNFSIPPGSLQNWRFRIRCVSATTGRFDAWFHNSGFARFRSHPLVQA
QGTIGIAATARGCIAVASHVSKNSWDSDDGPQQDLMAVMGRSSSFSSLGPTRDGRLKPEISAPGQYITAALAENSDLSDV
SVRADETNSLLTIEGTSMSAPVVTGAIALMLQKNPSLTPEQVKEILKESAKKDAHTQDTAWTPAYGYGKLDIAAAVSRV
>Mature_558_residues
SDAETPTLPQDIVAVSIKVSDKTAFDASYPTLAPEEQLVPIGDEFYSGKLSVSHLQGLLSHANVERIETKKTHSLTLDRA
STDIELLTAGGSRSIPQTGKDVLVGIVDSGFDLSHPMFRDVSGNLRVVALLDQTNGNQEYNTSQLQTQWGAGGSRPGEDK
DGHGTHVASIAGGSKLDQYEGIAPDAKFLLVKTDFTNVDLAVSWIYDQADGNPCVVNLSLGYHFGAHDGTSNEERLYDTL
VGPGKILVVSAGNERNDSIHLNGMFVPNQLEEVVFDILPRNSAGVPPFVATTFWYPDNDDYELDLITPSGQALPTPTLGN
TDSYSAGNLSIEISRTAYTISDLTQIQISISFSNFSIPPGSLQNWRFRIRCVSATTGRFDAWFHNSGFARFRSHPLVQAQ
GTIGIAATARGCIAVASHVSKNSWDSDDGPQQDLMAVMGRSSSFSSLGPTRDGRLKPEISAPGQYITAALAENSDLSDVS
VRADETNSLLTIEGTSMSAPVVTGAIALMLQKNPSLTPEQVKEILKESAKKDAHTQDTAWTPAYGYGKLDIAAAVSRV

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 59996; Mature: 59865

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAETPTLPQDIVAVSIKVSDKTAFDASYPTLAPEEQLVPIGDEFYSGKLSVSHLQGLL
CCCCCCCCCCCCEEEEEEEECCCCEECCCCCCCCCCCCEEECCCHHCCCCEEHHHHHHHH
SHANVERIETKKTHSLTLDRASTDIELLTAGGSRSIPQTGKDVLVGIVDSGFDLSHPMFR
HHCCHHHHHCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCHHH
DVSGNLRVVALLDQTNGNQEYNTSQLQTQWGAGGSRPGEDKDGHGTHVASIAGGSKLDQY
CCCCCEEEEEEEECCCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHH
EGIAPDAKFLLVKTDFTNVDLAVSWIYDQADGNPCVVNLSLGYHFGAHDGTSNEERLYDT
CCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEEEEEECEEECCCCCCCCHHHHHHH
LVGPGKILVVSAGNERNDSIHLNGMFVPNQLEEVVFDILPRNSAGVPPFVATTFWYPDND
HCCCCEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
DYELDLITPSGQALPTPTLGNTDSYSAGNLSIEISRTAYTISDLTQIQISISFSNFSIPP
CEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECEEEEECCEEEEEEEEEECCCCCCC
GSLQNWRFRIRCVSATTGRFDAWFHNSGFARFRSHPLVQAQGTIGIAATARGCIAVASHV
CCCCCCEEEEEEEEECCCCEEEEEECCCCHHHCCCCCEEECCCEEEEECCCHHHHHHHHH
SKNSWDSDDGPQQDLMAVMGRSSSFSSLGPTRDGRLKPEISAPGQYITAALAENSDLSDV
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCE
SVRADETNSLLTIEGTSMSAPVVTGAIALMLQKNPSLTPEQVKEILKESAKKDAHTQDTA
EEEECCCCCEEEEECCCCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCC
WTPAYGYGKLDIAAAVSRV
CCCCCCCCCEEHHHHHHCC
>Mature Secondary Structure 
SDAETPTLPQDIVAVSIKVSDKTAFDASYPTLAPEEQLVPIGDEFYSGKLSVSHLQGLL
CCCCCCCCCCCEEEEEEEECCCCEECCCCCCCCCCCCEEECCCHHCCCCEEHHHHHHHH
SHANVERIETKKTHSLTLDRASTDIELLTAGGSRSIPQTGKDVLVGIVDSGFDLSHPMFR
HHCCHHHHHCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCHHH
DVSGNLRVVALLDQTNGNQEYNTSQLQTQWGAGGSRPGEDKDGHGTHVASIAGGSKLDQY
CCCCCEEEEEEEECCCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHH
EGIAPDAKFLLVKTDFTNVDLAVSWIYDQADGNPCVVNLSLGYHFGAHDGTSNEERLYDT
CCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEEEEEECEEECCCCCCCCHHHHHHH
LVGPGKILVVSAGNERNDSIHLNGMFVPNQLEEVVFDILPRNSAGVPPFVATTFWYPDND
HCCCCEEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
DYELDLITPSGQALPTPTLGNTDSYSAGNLSIEISRTAYTISDLTQIQISISFSNFSIPP
CEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECEEEEECCEEEEEEEEEECCCCCCC
GSLQNWRFRIRCVSATTGRFDAWFHNSGFARFRSHPLVQAQGTIGIAATARGCIAVASHV
CCCCCCEEEEEEEEECCCCEEEEEECCCCHHHCCCCCEEECCCEEEEECCCHHHHHHHHH
SKNSWDSDDGPQQDLMAVMGRSSSFSSLGPTRDGRLKPEISAPGQYITAALAENSDLSDV
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCE
SVRADETNSLLTIEGTSMSAPVVTGAIALMLQKNPSLTPEQVKEILKESAKKDAHTQDTA
EEEECCCCCEEEEECCCCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCC
WTPAYGYGKLDIAAAVSRV
CCCCCCCCCEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]