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The map label for this gene is pilG [H]

Identifier: 158334811

GI number: 158334811

Start: 1615936

End: 1617081

Strand: Reverse

Name: pilG [H]

Synonym: AM1_1648

Alternate gene names: 158334811

Gene position: 1617081-1615936 (Counterclockwise)

Preceding gene: 158334812

Following gene: 158334810

Centisome position: 24.86

GC content: 47.56

Gene sequence:

>1146_bases
ATGACCTATACCTCCAGTCTTCCAACACCCACCCAACAGGACAAACCACAGGTCGCACCTGCCAAAGTGCTACAGAAAGT
GATCTCTGAGCAACGGTCTGGCCGCATCACTATCACTGATTCTCGTGATCCGTCGATTAGCTGGCGGGTGTATCTAGGCG
GTGGGCAAATGCATTTTGCAGAGAGCACAATGGGACATGCCGAGCGATTACCCTACGTGCTCAAAAAGCAGCTACCCGAC
TTTAATTTGTCTGAGCCTCTACCGAATAATACGTCAGAGTATTCCTATCTGTGCAAGTACTGGCAGGCCAATAATTTACC
CCTAAATTTCTTCCGAAAACTGCTCGCGATTCTGACCCAAGAAGCATTAATGCAGTTCTTAGCCCTCCCCCAAGGCTACT
TAGAATTTGAGCCCAATGTGGGGTTGGATCCACTATTGCTTTCTGCTCCGTTTCGCCAGCTCGTTTTACCGGTCCGAGAT
CAAATTAATCAATGGGCACAGCTGCAGGCTGTGATTGGCTCTCCCTTCCAAAAGCCTCATTTAGAGGATCCCGATCAATT
CTTGAAATTAGCTTGGAAGGAAAATGGCCAAGGACGACACTTACAAAATATTGTCAATCTATTAGAACAAGACCTTTGCT
TCTATGAGTTAGCCACCCAGGCAGATTTGGATGTCTTGACTCTGGTGACTGATTTAGCGCCGATGGTTCAGGCTGGAGCC
ATCAGTCTGAACCCTTATCGAGTCATCGAAGAGCAAGTTCGCCCCGTGGTTGCTTGTGTAGATGATAGTCAAACCATTCA
ACAATTTGTGAAACTGTCCCTAGAGGCAGAAGGCTTTGAAGTCCTGCAATTGATGGATCCGAAACAGGCATTAACGGTCT
TAATTGAGCAACAACCCAACGTCATCCTTATGGACATTGAAATGCCGAAGATGGATGGGTATGAGCTGTGTCGCATGGTT
CGCCAAGTCGATATGCTCAAGGAAATTCCTGTTGTGATGTTGACAGGTCGAGAAGGTCTGATTGACCGGATGAGAGCCCG
GATGTCTGGTTGTACGGCCTATTTGACCAAGCCATTTAATCCTCAAGAACTATTAGCCTTGGTACAAAAACTGTCGGCTG
AAGGCGCAGCCGTATCCCAGATGTAG

Upstream 100 bases:

>100_bases
GTACCTGAAAAAGTTGTTCTAGATCAAATCTGCCACTTGCCATCTTGCTGAGCTGGTAACTGCCAATGCTCTGACTATTA
ACTACCAAATCAGTTAGTTT

Downstream 100 bases:

>100_bases
TGTTCAAGAGTTACTGTGCACAGTAATCGCTGCATCACAACCATTAGTGCAATCGCCCAACAGGGCTTATTACCACGCAA
GTTCCTTTTCAGTGCGCGTC

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTYTSSLPTPTQQDKPQVAPAKVLQKVISEQRSGRITITDSRDPSISWRVYLGGGQMHFAESTMGHAERLPYVLKKQLPD
FNLSEPLPNNTSEYSYLCKYWQANNLPLNFFRKLLAILTQEALMQFLALPQGYLEFEPNVGLDPLLLSAPFRQLVLPVRD
QINQWAQLQAVIGSPFQKPHLEDPDQFLKLAWKENGQGRHLQNIVNLLEQDLCFYELATQADLDVLTLVTDLAPMVQAGA
ISLNPYRVIEEQVRPVVACVDDSQTIQQFVKLSLEAEGFEVLQLMDPKQALTVLIEQQPNVILMDIEMPKMDGYELCRMV
RQVDMLKEIPVVMLTGREGLIDRMRARMSGCTAYLTKPFNPQELLALVQKLSAEGAAVSQM

Sequences:

>Translated_381_residues
MTYTSSLPTPTQQDKPQVAPAKVLQKVISEQRSGRITITDSRDPSISWRVYLGGGQMHFAESTMGHAERLPYVLKKQLPD
FNLSEPLPNNTSEYSYLCKYWQANNLPLNFFRKLLAILTQEALMQFLALPQGYLEFEPNVGLDPLLLSAPFRQLVLPVRD
QINQWAQLQAVIGSPFQKPHLEDPDQFLKLAWKENGQGRHLQNIVNLLEQDLCFYELATQADLDVLTLVTDLAPMVQAGA
ISLNPYRVIEEQVRPVVACVDDSQTIQQFVKLSLEAEGFEVLQLMDPKQALTVLIEQQPNVILMDIEMPKMDGYELCRMV
RQVDMLKEIPVVMLTGREGLIDRMRARMSGCTAYLTKPFNPQELLALVQKLSAEGAAVSQM
>Mature_380_residues
TYTSSLPTPTQQDKPQVAPAKVLQKVISEQRSGRITITDSRDPSISWRVYLGGGQMHFAESTMGHAERLPYVLKKQLPDF
NLSEPLPNNTSEYSYLCKYWQANNLPLNFFRKLLAILTQEALMQFLALPQGYLEFEPNVGLDPLLLSAPFRQLVLPVRDQ
INQWAQLQAVIGSPFQKPHLEDPDQFLKLAWKENGQGRHLQNIVNLLEQDLCFYELATQADLDVLTLVTDLAPMVQAGAI
SLNPYRVIEEQVRPVVACVDDSQTIQQFVKLSLEAEGFEVLQLMDPKQALTVLIEQQPNVILMDIEMPKMDGYELCRMVR
QVDMLKEIPVVMLTGREGLIDRMRARMSGCTAYLTKPFNPQELLALVQKLSAEGAAVSQM

Specific function: Is involved in pilus biosynthesis and twitching motility. May act as a single-domain response regulator whose function may be to receive a certain environmental signal(s) and then transduce that signal to the pilus assembly machinery via specific protein-

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=34.7826086956522, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082012, Length=113, Percent_Identity=32.7433628318584, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790863, Length=117, Percent_Identity=31.6239316239316, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786911, Length=110, Percent_Identity=32.7272727272727, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787375, Length=123, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=31.6239316239316, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=33.0508474576271, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789809, Length=113, Percent_Identity=28.3185840707965, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788394, Length=117, Percent_Identity=29.9145299145299, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 43088; Mature: 42956

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYTSSLPTPTQQDKPQVAPAKVLQKVISEQRSGRITITDSRDPSISWRVYLGGGQMHFA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCCEEEEH
ESTMGHAERLPYVLKKQLPDFNLSEPLPNNTSEYSYLCKYWQANNLPLNFFRKLLAILTQ
HHHHCHHHHCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EALMQFLALPQGYLEFEPNVGLDPLLLSAPFRQLVLPVRDQINQWAQLQAVIGSPFQKPH
HHHHHHHHCCCCCEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LEDPDQFLKLAWKENGQGRHLQNIVNLLEQDLCFYELATQADLDVLTLVTDLAPMVQAGA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
ISLNPYRVIEEQVRPVVACVDDSQTIQQFVKLSLEAEGFEVLQLMDPKQALTVLIEQQPN
CCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCC
VILMDIEMPKMDGYELCRMVRQVDMLKEIPVVMLTGREGLIDRMRARMSGCTAYLTKPFN
EEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCEEECCCCC
PQELLALVQKLSAEGAAVSQM
HHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure 
TYTSSLPTPTQQDKPQVAPAKVLQKVISEQRSGRITITDSRDPSISWRVYLGGGQMHFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCCEEEEH
ESTMGHAERLPYVLKKQLPDFNLSEPLPNNTSEYSYLCKYWQANNLPLNFFRKLLAILTQ
HHHHCHHHHCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EALMQFLALPQGYLEFEPNVGLDPLLLSAPFRQLVLPVRDQINQWAQLQAVIGSPFQKPH
HHHHHHHHCCCCCEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LEDPDQFLKLAWKENGQGRHLQNIVNLLEQDLCFYELATQADLDVLTLVTDLAPMVQAGA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
ISLNPYRVIEEQVRPVVACVDDSQTIQQFVKLSLEAEGFEVLQLMDPKQALTVLIEQQPN
CCCCHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCC
VILMDIEMPKMDGYELCRMVRQVDMLKEIPVVMLTGREGLIDRMRARMSGCTAYLTKPFN
EEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCEEECCCCC
PQELLALVQKLSAEGAAVSQM
HHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8104179; 10984043 [H]