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The map label for this gene is ppsA [H]

Identifier: 158334808

GI number: 158334808

Start: 1612017

End: 1614467

Strand: Direct

Name: ppsA [H]

Synonym: AM1_1645

Alternate gene names: 158334808

Gene position: 1612017-1614467 (Clockwise)

Preceding gene: 158334802

Following gene: 158334809

Centisome position: 24.79

GC content: 51.61

Gene sequence:

>2451_bases
ATGCTTCCTATCTCTCCCCAACAACCGCCAACAACCAAACATCAGTCCTTCATTCTTTGGTTTGATCAGGTTGGCATTGC
AGACATTCCCCTGGTTGGCGGCAAGAATGCCTCCTTAGGAGAGATGATCCAACAGCTAGGAGCGCAAGGTGTCCAAGTAC
CCACCGGCTTTGCCACCACAGCCCATGCCTATCGAACCTTTCTGCAACAATCCCAACTAGAAGCCCCCCTTCGTCAGCTC
TTACAAGGGTTAGACGTAGAAGATATTCCACAGCTCCGAGCACGGGCACAGCAGGCCCGCGAACTGATCCTGGCCACTCC
TTTTTCTCCCGGATTAGAACTAGCCATCAGTGAAGCGTATTCCAGACTCTGTCAGCACTGTGGTGAACAGTCATCCTTAT
CAGTAGCCGTTCGCTCCAGCGCCACCGCTGAGGATTTACCGGATGCCAGCTTCGCGGGGCAACAAGAAACCTATCTCAAT
GTCCAAGGCCTTCGCGGGGTCTTAGAAGCCTGTCATAAATGTTTTGCCTCTCTCTTTACCGATCGCGCAATTTCCTATCG
CACCTTAAAAGGGTTTGACCACTTCACAGTGGCCTTAGCTGTAGGGGTCCAGCAAATGGTGCGATCTGGCACCGCTGGGG
TGATGTTCTCCATCGATACCGAAACTGGGTTTGATCAAGCAGTCCTGATCACGGCAGCCTACGGGCTCGGGGAAACCGTG
GTCCAAGGTGCCGTTAACCCAGACGAATATTTGGTGTTTAAACCAACACTCCAACAGGGCTATCAGCCCATTCTATCTAA
GCGACTCGGCAGTAAAGAGATCAAAATGGTTTACGACCAAGAGGGCAGTAAACTCACGAAAAATGTGCCCGTATCTGCAG
GCGATCGCCAGAAATTTACCCTCACCAACCCAGAAATTCTTACCCTCGCCCAATGGGCTTGCAAAATTGAAGATCATTAT
TCTGCCGTGCGGGGCACCTACACCCCCATGGATATTGAATGGGCCCAGGATGAGTTCACCGGGGAGCTATACATCGTCCA
AGCCCGACCGGAGACCGTACAGTCGCAAAAATCAGACACCCTGCTAAGAACGTACCAGTTAGATACGACCGGGTCAGTCC
TGAGCACCGGTCGGGCCGTAGGGGAATTGATTGGCCAGGGGCGAGGGCAAATCATTCAACATGTCAGCCAAATCAGTGCG
TTTCAGCCTGGGGACGTCTTAATCACCCATAAAACGGATCCCGATTGGGAACCGATTATGAAGCAGGCCGCAGCCATCGT
TACCAACCAAGGAGGGCGCACCTGCCATGCCGCGATTATTGCTCGGGAATTGGGCATCCCTGCCATTGTCGGGTGTGGTA
ATGCCACCCACCAGATCCCCAACGGTCGGCTGGTCACGGTTGCCTGCTGTGAAGGGGAAGTCGGCCATGTTTACGATGGG
CTACTGTCCTTTACCGTTCAAGAAACCCAACTAGAACAGCTCCCCACCACCCAAACTAAAATCCTGCTCAATGTGGGGAA
TCCTGACCAAGCCTTTAGACTGGCAGCCCTGCCCTGTGATGGCGTGGGCATCGCCCGCTTAGAATTTATTATTGCCAACC
ATATCAAAGCCCATCCCCTGGCCTTAATCCATTTCGATCAACTGGACGATCCGTTAGCTCAACAAGAAATTACCCACCTG
ACTCGCCACTATCCCTACAAACCGGACTTCTTTGTGGATCAGCTCACCCATGGGGTAGGCATTATTGCCGCAGCGTTTTA
TCCCAAACCTGTCATTGTCAGACTGTCTGATTTCAAGAGTAATGAATATGCAAACTTACTCGGCGGTCAGCAATTTGAAC
CTCAGGAAGAAAATCCCATGCTGGGATGGCGGGGGGCATCTCGTTACTACGACGATAAGTTTCGAGAAGCATTTGGTTTG
GAGTGTAAAGCCCTCAAGATCGTCCGAGACGAGATGGGCTTAACCAACGTGATCCCCATGGTGCCCTTCTGCCGAACACC
AGAAGAAGGTCGCAAAGTTATTGCAGAAATGGAGAATGCTGGATTAGTCCAGGGGGAAAATGGCCTCCAGGTTTATGTGA
TGTGTGAAGTACCCAGCAACGTCATTCTGGCGGATCAATTCAGCCAAATCTTTGATGGGTTTTCCATCGGCTCCAACGAT
CTCACCCAATTAGCCCTAGGGATTGATCGAGACTCAGCCTTAGTCGCCCACCTCTTTGATGAACGTCATGAAGGTGTGCA
ATCCCTGATTCGTCAAGTGATTGACCAGGCCAAACGCCATCACTGCAAAGTCGGCATCTGTGGCCAAGCCCCTAGCGACT
ACCCAGAATTTGCCCAATTCCTCGTTGAACAAGGAATTGACTCCATCAGCCTCAATCCTGATTCAGTGCTCAAAACAAGG
TTCACTATTGCCCAAGTTGAAACACACCTGTCATCGTCTCAACCGTCCTAG

Upstream 100 bases:

>100_bases
GAGGTCTTCAAAAATCCTCACAAGTTTCTCAAATTAGCGGGATATAAAGAAGACAAATATTCTGATTCCGATCTTCTCCA
GAGAGCGGGACCTAGCCCCC

Downstream 100 bases:

>100_bases
TCTTATATCGTTTTCCACGCCCCTCGGAGGTACTCATCATGTTCCGCAAGATATTAGTTGCCCTTGACCATTCAGAAACA
GCTCTGGATGTCTTTGATCA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 816; Mature: 816

Protein sequence:

>816_residues
MLPISPQQPPTTKHQSFILWFDQVGIADIPLVGGKNASLGEMIQQLGAQGVQVPTGFATTAHAYRTFLQQSQLEAPLRQL
LQGLDVEDIPQLRARAQQARELILATPFSPGLELAISEAYSRLCQHCGEQSSLSVAVRSSATAEDLPDASFAGQQETYLN
VQGLRGVLEACHKCFASLFTDRAISYRTLKGFDHFTVALAVGVQQMVRSGTAGVMFSIDTETGFDQAVLITAAYGLGETV
VQGAVNPDEYLVFKPTLQQGYQPILSKRLGSKEIKMVYDQEGSKLTKNVPVSAGDRQKFTLTNPEILTLAQWACKIEDHY
SAVRGTYTPMDIEWAQDEFTGELYIVQARPETVQSQKSDTLLRTYQLDTTGSVLSTGRAVGELIGQGRGQIIQHVSQISA
FQPGDVLITHKTDPDWEPIMKQAAAIVTNQGGRTCHAAIIARELGIPAIVGCGNATHQIPNGRLVTVACCEGEVGHVYDG
LLSFTVQETQLEQLPTTQTKILLNVGNPDQAFRLAALPCDGVGIARLEFIIANHIKAHPLALIHFDQLDDPLAQQEITHL
TRHYPYKPDFFVDQLTHGVGIIAAAFYPKPVIVRLSDFKSNEYANLLGGQQFEPQEENPMLGWRGASRYYDDKFREAFGL
ECKALKIVRDEMGLTNVIPMVPFCRTPEEGRKVIAEMENAGLVQGENGLQVYVMCEVPSNVILADQFSQIFDGFSIGSND
LTQLALGIDRDSALVAHLFDERHEGVQSLIRQVIDQAKRHHCKVGICGQAPSDYPEFAQFLVEQGIDSISLNPDSVLKTR
FTIAQVETHLSSSQPS

Sequences:

>Translated_816_residues
MLPISPQQPPTTKHQSFILWFDQVGIADIPLVGGKNASLGEMIQQLGAQGVQVPTGFATTAHAYRTFLQQSQLEAPLRQL
LQGLDVEDIPQLRARAQQARELILATPFSPGLELAISEAYSRLCQHCGEQSSLSVAVRSSATAEDLPDASFAGQQETYLN
VQGLRGVLEACHKCFASLFTDRAISYRTLKGFDHFTVALAVGVQQMVRSGTAGVMFSIDTETGFDQAVLITAAYGLGETV
VQGAVNPDEYLVFKPTLQQGYQPILSKRLGSKEIKMVYDQEGSKLTKNVPVSAGDRQKFTLTNPEILTLAQWACKIEDHY
SAVRGTYTPMDIEWAQDEFTGELYIVQARPETVQSQKSDTLLRTYQLDTTGSVLSTGRAVGELIGQGRGQIIQHVSQISA
FQPGDVLITHKTDPDWEPIMKQAAAIVTNQGGRTCHAAIIARELGIPAIVGCGNATHQIPNGRLVTVACCEGEVGHVYDG
LLSFTVQETQLEQLPTTQTKILLNVGNPDQAFRLAALPCDGVGIARLEFIIANHIKAHPLALIHFDQLDDPLAQQEITHL
TRHYPYKPDFFVDQLTHGVGIIAAAFYPKPVIVRLSDFKSNEYANLLGGQQFEPQEENPMLGWRGASRYYDDKFREAFGL
ECKALKIVRDEMGLTNVIPMVPFCRTPEEGRKVIAEMENAGLVQGENGLQVYVMCEVPSNVILADQFSQIFDGFSIGSND
LTQLALGIDRDSALVAHLFDERHEGVQSLIRQVIDQAKRHHCKVGICGQAPSDYPEFAQFLVEQGIDSISLNPDSVLKTR
FTIAQVETHLSSSQPS
>Mature_816_residues
MLPISPQQPPTTKHQSFILWFDQVGIADIPLVGGKNASLGEMIQQLGAQGVQVPTGFATTAHAYRTFLQQSQLEAPLRQL
LQGLDVEDIPQLRARAQQARELILATPFSPGLELAISEAYSRLCQHCGEQSSLSVAVRSSATAEDLPDASFAGQQETYLN
VQGLRGVLEACHKCFASLFTDRAISYRTLKGFDHFTVALAVGVQQMVRSGTAGVMFSIDTETGFDQAVLITAAYGLGETV
VQGAVNPDEYLVFKPTLQQGYQPILSKRLGSKEIKMVYDQEGSKLTKNVPVSAGDRQKFTLTNPEILTLAQWACKIEDHY
SAVRGTYTPMDIEWAQDEFTGELYIVQARPETVQSQKSDTLLRTYQLDTTGSVLSTGRAVGELIGQGRGQIIQHVSQISA
FQPGDVLITHKTDPDWEPIMKQAAAIVTNQGGRTCHAAIIARELGIPAIVGCGNATHQIPNGRLVTVACCEGEVGHVYDG
LLSFTVQETQLEQLPTTQTKILLNVGNPDQAFRLAALPCDGVGIARLEFIIANHIKAHPLALIHFDQLDDPLAQQEITHL
TRHYPYKPDFFVDQLTHGVGIIAAAFYPKPVIVRLSDFKSNEYANLLGGQQFEPQEENPMLGWRGASRYYDDKFREAFGL
ECKALKIVRDEMGLTNVIPMVPFCRTPEEGRKVIAEMENAGLVQGENGLQVYVMCEVPSNVILADQFSQIFDGFSIGSND
LTQLALGIDRDSALVAHLFDERHEGVQSLIRQVIDQAKRHHCKVGICGQAPSDYPEFAQFLVEQGIDSISLNPDSVLKTR
FTIAQVETHLSSSQPS

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=795, Percent_Identity=55.2201257861635, Blast_Score=899, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=458, Percent_Identity=26.8558951965066, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI1789193, Length=408, Percent_Identity=25.7352941176471, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1788726, Length=191, Percent_Identity=32.9842931937173, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI48994992, Length=371, Percent_Identity=22.911051212938, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17564524, Length=240, Percent_Identity=29.5833333333333, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 89518; Mature: 89518

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPISPQQPPTTKHQSFILWFDQVGIADIPLVGGKNASLGEMIQQLGAQGVQVPTGFATT
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCHHHHHHHHCCCCCCCCCCCHHH
AHAYRTFLQQSQLEAPLRQLLQGLDVEDIPQLRARAQQARELILATPFSPGLELAISEAY
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHH
SRLCQHCGEQSSLSVAVRSSATAEDLPDASFAGQQETYLNVQGLRGVLEACHKCFASLFT
HHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
DRAISYRTLKGFDHFTVALAVGVQQMVRSGTAGVMFSIDTETGFDQAVLITAAYGLGETV
HHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCHHH
VQGAVNPDEYLVFKPTLQQGYQPILSKRLGSKEIKMVYDQEGSKLTKNVPVSAGDRQKFT
HHCCCCCCCEEEECCCHHHCHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCCCCCCEEE
LTNPEILTLAQWACKIEDHYSAVRGTYTPMDIEWAQDEFTGELYIVQARPETVQSQKSDT
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEECCCHHHHCCCCCC
LLRTYQLDTTGSVLSTGRAVGELIGQGRGQIIQHVSQISAFQPGDVLITHKTDPDWEPIM
EEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
KQAAAIVTNQGGRTCHAAIIARELGIPAIVGCGNATHQIPNGRLVTVACCEGEVGHVYDG
HHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHH
LLSFTVQETQLEQLPTTQTKILLNVGNPDQAFRLAALPCDGVGIARLEFIIANHIKAHPL
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCE
ALIHFDQLDDPLAQQEITHLTRHYPYKPDFFVDQLTHGVGIIAAAFYPKPVIVRLSDFKS
EEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCEEEEEECCCC
NEYANLLGGQQFEPQEENPMLGWRGASRYYDDKFREAFGLECKALKIVRDEMGLTNVIPM
CCHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCC
VPFCRTPEEGRKVIAEMENAGLVQGENGLQVYVMCEVPSNVILADQFSQIFDGFSIGSND
CCCCCCCHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCCH
LTQLALGIDRDSALVAHLFDERHEGVQSLIRQVIDQAKRHHCKVGICGQAPSDYPEFAQF
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
LVEQGIDSISLNPDSVLKTRFTIAQVETHLSSSQPS
HHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLPISPQQPPTTKHQSFILWFDQVGIADIPLVGGKNASLGEMIQQLGAQGVQVPTGFATT
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCHHHHHHHHCCCCCCCCCCCHHH
AHAYRTFLQQSQLEAPLRQLLQGLDVEDIPQLRARAQQARELILATPFSPGLELAISEAY
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHH
SRLCQHCGEQSSLSVAVRSSATAEDLPDASFAGQQETYLNVQGLRGVLEACHKCFASLFT
HHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
DRAISYRTLKGFDHFTVALAVGVQQMVRSGTAGVMFSIDTETGFDQAVLITAAYGLGETV
HHCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCHHH
VQGAVNPDEYLVFKPTLQQGYQPILSKRLGSKEIKMVYDQEGSKLTKNVPVSAGDRQKFT
HHCCCCCCCEEEECCCHHHCHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCCCCCCEEE
LTNPEILTLAQWACKIEDHYSAVRGTYTPMDIEWAQDEFTGELYIVQARPETVQSQKSDT
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEECCCHHHHCCCCCC
LLRTYQLDTTGSVLSTGRAVGELIGQGRGQIIQHVSQISAFQPGDVLITHKTDPDWEPIM
EEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
KQAAAIVTNQGGRTCHAAIIARELGIPAIVGCGNATHQIPNGRLVTVACCEGEVGHVYDG
HHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHH
LLSFTVQETQLEQLPTTQTKILLNVGNPDQAFRLAALPCDGVGIARLEFIIANHIKAHPL
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCE
ALIHFDQLDDPLAQQEITHLTRHYPYKPDFFVDQLTHGVGIIAAAFYPKPVIVRLSDFKS
EEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCEEEEEECCCC
NEYANLLGGQQFEPQEENPMLGWRGASRYYDDKFREAFGLECKALKIVRDEMGLTNVIPM
CCHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCC
VPFCRTPEEGRKVIAEMENAGLVQGENGLQVYVMCEVPSNVILADQFSQIFDGFSIGSND
CCCCCCCHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCCH
LTQLALGIDRDSALVAHLFDERHEGVQSLIRQVIDQAKRHHCKVGICGQAPSDYPEFAQF
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
LVEQGIDSISLNPDSVLKTRFTIAQVETHLSSSQPS
HHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]