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The map label for this gene is yfiK [H]

Identifier: 158334732

GI number: 158334732

Start: 1550517

End: 1551164

Strand: Direct

Name: yfiK [H]

Synonym: AM1_1566

Alternate gene names: 158334732

Gene position: 1550517-1551164 (Clockwise)

Preceding gene: 158334731

Following gene: 158334733

Centisome position: 23.84

GC content: 49.23

Gene sequence:

>648_bases
ATGATTCGGCTACTGCTTGCAGATGATCAGGCTCTATTTCGCCAAGGGCTGGCCTCACTTTTAGAGCTAGAGGAAGATTT
ATCCGTTGTGGGGCAGGCACAAGATGGACGAGATGCCATCCTATTGACCCAAACCCTACAGCCTGATGTGATTCTTATGG
ATGTACGGATGCCAATCTGTGATGGTGTGGAGGCTACCCGAGAGATCCATCAACGGTTTCCTTGGATTCGTATTTTGGTC
TTGACCACCTTTGATGAAGACGAATATATCTGGACATCATTGCAGGCAGGGGCTTTGGGTTATTTACTGAAGAGTACACC
CGCTAGTCAGCTTGCAGCAGCAATACGGATGCTGCACGATGGCCACTTTCAACTCGGACCGACTATTGCCCCAAAGGTGA
TTGCCCAATTGCGCCCCCCTCCTCTCAAAAATGAGCAAAACCACTTGGAACGGTTGAGTGACCGCGAACTGGATGTCCTT
AACTTGATCGGCCAGGGCAAGAGTAATCGGGAAATCTCACAGATTTTGCATTTAACCGAAGGCACCGTTAAAAATTATGT
GAGTCAGTTGTTAGGGAGTTTGGGGGTTCGTGATCGCACCCAAGCGGCCCTATGGGCACAGCAATATCTATCCCCACCTC
AAACGTAA

Upstream 100 bases:

>100_bases
ACGAGCGCACTCAAATCCTAGGTGGAGTGTTTACCATTCAAAGTTATCCGGGTGAAGGAACCACCTTACAGATCGTCATT
CCATATCCGTCAGAGTCATC

Downstream 100 bases:

>100_bases
ATGGCTGATGCCAAATAGAAGAAGTGACGAATGTCACCTTTATCTATGACTTTTAGGAGCGGTAGAACCTATCAGCCAAC
CCCTAAGGTAATCAGGAGAT

Product: two-component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIRLLLADDQALFRQGLASLLELEEDLSVVGQAQDGRDAILLTQTLQPDVILMDVRMPICDGVEATREIHQRFPWIRILV
LTTFDEDEYIWTSLQAGALGYLLKSTPASQLAAAIRMLHDGHFQLGPTIAPKVIAQLRPPPLKNEQNHLERLSDRELDVL
NLIGQGKSNREISQILHLTEGTVKNYVSQLLGSLGVRDRTQAALWAQQYLSPPQT

Sequences:

>Translated_215_residues
MIRLLLADDQALFRQGLASLLELEEDLSVVGQAQDGRDAILLTQTLQPDVILMDVRMPICDGVEATREIHQRFPWIRILV
LTTFDEDEYIWTSLQAGALGYLLKSTPASQLAAAIRMLHDGHFQLGPTIAPKVIAQLRPPPLKNEQNHLERLSDRELDVL
NLIGQGKSNREISQILHLTEGTVKNYVSQLLGSLGVRDRTQAALWAQQYLSPPQT
>Mature_215_residues
MIRLLLADDQALFRQGLASLLELEEDLSVVGQAQDGRDAILLTQTLQPDVILMDVRMPICDGVEATREIHQRFPWIRILV
LTTFDEDEYIWTSLQAGALGYLLKSTPASQLAAAIRMLHDGHFQLGPTIAPKVIAQLRPPPLKNEQNHLERLSDRELDVL
NLIGQGKSNREISQILHLTEGTVKNYVSQLLGSLGVRDRTQAALWAQQYLSPPQT

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=197, Percent_Identity=34.5177664974619, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=34.9282296650718, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787473, Length=205, Percent_Identity=34.6341463414634, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1786747, Length=207, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790102, Length=203, Percent_Identity=30.5418719211823, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=24.0196078431373, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082052, Length=116, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24063; Mature: 24063

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLLLADDQALFRQGLASLLELEEDLSVVGQAQDGRDAILLTQTLQPDVILMDVRMPIC
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEECCCCCC
DGVEATREIHQRFPWIRILVLTTFDEDEYIWTSLQAGALGYLLKSTPASQLAAAIRMLHD
CCHHHHHHHHHHCCEEEEEEEEECCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
GHFQLGPTIAPKVIAQLRPPPLKNEQNHLERLSDRELDVLNLIGQGKSNREISQILHLTE
CCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHH
GTVKNYVSQLLGSLGVRDRTQAALWAQQYLSPPQT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIRLLLADDQALFRQGLASLLELEEDLSVVGQAQDGRDAILLTQTLQPDVILMDVRMPIC
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEECCCCCC
DGVEATREIHQRFPWIRILVLTTFDEDEYIWTSLQAGALGYLLKSTPASQLAAAIRMLHD
CCHHHHHHHHHHCCEEEEEEEEECCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
GHFQLGPTIAPKVIAQLRPPPLKNEQNHLERLSDRELDVLNLIGQGKSNREISQILHLTE
CCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHH
GTVKNYVSQLLGSLGVRDRTQAALWAQQYLSPPQT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]