Click here to switch to the map view.

The map label for this gene is hisA

Identifier: 158334681

GI number: 158334681

Start: 1501652

End: 1502425

Strand: Direct

Name: hisA

Synonym: AM1_1514

Alternate gene names: 158334681

Gene position: 1501652-1502425 (Clockwise)

Preceding gene: 158334679

Following gene: 158334695

Centisome position: 23.09

GC content: 51.81

Gene sequence:

>774_bases
ATGGATGTTATCCCTGCTATTGACTTGTTAGAGGGACGATGCGTTCGTCTATATCAAGGGGACTATGACCAATCCCAGGT
TTTTTCTGAAAACCCTGTTGAAGTTGCCAAGGCATGGGAGGCTCAGGGTGCCCAATGGCTGCATTTGGTGGATTTAGATG
GTGCTAAAACAGGCCAGCCCGTGAATTTAGACACCATTGCTGCGGTGGTGGAGGCTTTAGAGATTCCGGTGCAAGTGGGG
GGTGGCTTGCGAGATCGCAACCGCGTTACCCAACTTCTAAATTTAGGTGTAAGAAGAGGTATTTTAGGTACGGTAGCCAT
CGAAAACCCCGATCTGGTGCAGGAACTATGCCAGGAATTTCCCGATCAGATTGTGGTGGGGATTGATGCAAGGGAAGGCA
GAGTAGCCACCCGAGGCTGGTTAGAAACCTCTGAGATTCTGGCGGTAGACTTAGCCAAACGCATGGTGACGGCGGGTGCA
GCGGCGATTATTTACACGGATATTCAGCGGGATGGTACATTGCAAGGTCCTAATATTCCGATGCTACGAGAAATGGCTGA
GGCGATTACGATTCCGGTGATTGCTTCCGGTGGCGTCAGCTCAATTACAGATCTACTAAACTTGCTGGCATTGGAAGCTA
TCGGGGTGACGGGCGCCATTGTAGGCAAGGCTTTATATACAGAAGATATCGCGTTGTCTGAAGCGCTCCGGGCCGTTGGT
CCTGGTCGCTGGCAGGATGTGCCCCCCGATTTAGGCTCTTCTGCGCTGGCCTGA

Upstream 100 bases:

>100_bases
GGCCCAGCTGAAGCCAGCCGTTCAGGATTTGAAATGGCATGGGGCCTGGACCGATACAGAGTGATTCACGAAAATTTGGC
AACTGAAAGGTAAACGGTTT

Downstream 100 bases:

>100_bases
AAGTGACAACACCATTCTGTAAACTATTTGATCTGAGATATTGAGAAGCTCAATGGCTATTCAAGCTCAAATTCAACATT
AAGAAGAGGATGGGAGTGCT

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MDVIPAIDLLEGRCVRLYQGDYDQSQVFSENPVEVAKAWEAQGAQWLHLVDLDGAKTGQPVNLDTIAAVVEALEIPVQVG
GGLRDRNRVTQLLNLGVRRGILGTVAIENPDLVQELCQEFPDQIVVGIDAREGRVATRGWLETSEILAVDLAKRMVTAGA
AAIIYTDIQRDGTLQGPNIPMLREMAEAITIPVIASGGVSSITDLLNLLALEAIGVTGAIVGKALYTEDIALSEALRAVG
PGRWQDVPPDLGSSALA

Sequences:

>Translated_257_residues
MDVIPAIDLLEGRCVRLYQGDYDQSQVFSENPVEVAKAWEAQGAQWLHLVDLDGAKTGQPVNLDTIAAVVEALEIPVQVG
GGLRDRNRVTQLLNLGVRRGILGTVAIENPDLVQELCQEFPDQIVVGIDAREGRVATRGWLETSEILAVDLAKRMVTAGA
AAIIYTDIQRDGTLQGPNIPMLREMAEAITIPVIASGGVSSITDLLNLLALEAIGVTGAIVGKALYTEDIALSEALRAVG
PGRWQDVPPDLGSSALA
>Mature_257_residues
MDVIPAIDLLEGRCVRLYQGDYDQSQVFSENPVEVAKAWEAQGAQWLHLVDLDGAKTGQPVNLDTIAAVVEALEIPVQVG
GGLRDRNRVTQLLNLGVRRGILGTVAIENPDLVQELCQEFPDQIVVGIDAREGRVATRGWLETSEILAVDLAKRMVTAGA
AAIIYTDIQRDGTLQGPNIPMLREMAEAITIPVIASGGVSSITDLLNLLALEAIGVTGAIVGKALYTEDIALSEALRAVG
PGRWQDVPPDLGSSALA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=251, Percent_Identity=36.2549800796813, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1788336, Length=243, Percent_Identity=24.6913580246914, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_ACAM1 (B0C904)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515853.1
- ProteinModelPortal:   B0C904
- SMR:   B0C904
- GeneID:   5680334
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1514
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   AMAR329726:AM1_1514-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 27351; Mature: 27351

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVIPAIDLLEGRCVRLYQGDYDQSQVFSENPVEVAKAWEAQGAQWLHLVDLDGAKTGQP
CCCCCCHHHHCCCEEEEECCCCCHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCC
VNLDTIAAVVEALEIPVQVGGGLRDRNRVTQLLNLGVRRGILGTVAIENPDLVQELCQEF
CCHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHC
PDQIVVGIDAREGRVATRGWLETSEILAVDLAKRMVTAGAAAIIYTDIQRDGTLQGPNIP
CCCEEEEECCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCH
MLREMAEAITIPVIASGGVSSITDLLNLLALEAIGVTGAIVGKALYTEDIALSEALRAVG
HHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
PGRWQDVPPDLGSSALA
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDVIPAIDLLEGRCVRLYQGDYDQSQVFSENPVEVAKAWEAQGAQWLHLVDLDGAKTGQP
CCCCCCHHHHCCCEEEEECCCCCHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCC
VNLDTIAAVVEALEIPVQVGGGLRDRNRVTQLLNLGVRRGILGTVAIENPDLVQELCQEF
CCHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHC
PDQIVVGIDAREGRVATRGWLETSEILAVDLAKRMVTAGAAAIIYTDIQRDGTLQGPNIP
CCCEEEEECCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCH
MLREMAEAITIPVIASGGVSSITDLLNLLALEAIGVTGAIVGKALYTEDIALSEALRAVG
HHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
PGRWQDVPPDLGSSALA
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA