Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 158334577

GI number: 158334577

Start: 1386865

End: 1388364

Strand: Reverse

Name: murE [H]

Synonym: AM1_1404

Alternate gene names: 158334577

Gene position: 1388364-1386865 (Counterclockwise)

Preceding gene: 158334578

Following gene: 158334575

Centisome position: 21.35

GC content: 51.87

Gene sequence:

>1500_bases
TTGGGCATGGTCAAATTACGTACGTTACTCGCTGAACTGTCTACCCACCTGTGGGAGACGCCCCCCACTGACCATCCAGC
CCTAGATGCAGAGATTACGGGGTTAACGACCAATTCCTGGGCAGTCCAGCCAGGATATTTATTTATCGGTATGCCGGGTA
CCCGCGTGGATGGTGGTAGTTTTTGGAAAAGTGCAATTGAGGCGGGTGCTGCTGCTGCGTTGGTTTCTGATCAAGTGGAT
CGGGCGGATGTGGGTACCCACTGTGTGTTGGCCCTGAAGGATATGCCTCGGGCTTGTGCTGAGGTGGCTGCCACCTTTTA
TGATCAGCCAAGCCAGAAATTAAAGCTGGTGGGTATGACCGGAACGAATGGGAAAACCACGACCACCCATCTGATTGAAT
ATTTATTGACAACGGCCCAAGCTCCCACCGCTTTATTTGGCACCCTGTATGCCCGCTGGCCTGGGCATCAGGTGACGGCT
GCCCATACGACGCCTTTTGCCGTGGATTTACAGCAGCAATTGTCTGATGCGGTGGCGGCCCAGTGTCAGTTTGGGGTGAT
GGAAGTCAGCTCCCATGCTTTAGCCCAGAAACGGGTTCTGGGCTGCCAGTTTACGGTGGCTGTGTTTTCGAATTTGACTC
AAGATCACCTGGATTACCACCAGGATATGGAAGATTACTTTGCCGCTAAGGCGCTATTGTTCTCCCCGGACTATTTACAA
GGGCGAGCCATTATCAATATTGACGATGTCTACGGTCAGCGCTTAGTAGACCAGATCGGTCCGAATCAATGCTGGACTTA
CAGTATTGAGGGACCGGCGGATTTATATACCAGTGGTCTGTCCTATGAAACGGCTGGGGTTAAAGGACAACTGCATACCC
CGAAAGGTACGGTGGAGTTCTTTTCGTCTTTGGTGGGACAGTTTAATGTCTCTAATCTATTGGCGGCTGTCGGGGCTGTC
CTGGAATTAGGACTGACCTTAGAGCAAATCACCCCAGCTTTAGCTCAATTCCCTGGGGTGCCCGGTCGAATGCAGCAAAT
TACCTATTCTGCAGACCAGGATATTAGCGTGATTGTTGATTACGCCCATACCCCCGATAGTCTAGAGAATTTGTTGAAGG
CAGCCCGTCCCTTTGTTAAAGGCCAAATGGCCTGTGTCTTCGGTTGCGGGGGCGATCGCGATCGGACTAAAAGACCCTTA
ATGGGAGAAATTGCTGCTCGTCTTTCGGATCAGGCCATTGTCACGTCCGATAATCCACGAACTGAAGATCCTCAGCAGAT
TTTGCAGGATGTGTTAGCGGGTATCCCGATGGAGGTGAAGCCCATAGTGGAGGTGGATCGGCGAGTGGCGATTCAGGCTG
CGATCGCACAAGCAAAACCCGGTGATACTATCCTGATTGCAGGCAAGGGCCATGAAGACTACCAAATCCTAGGCACCGAA
AAAATCCATTTCGACGATCGCGAAGAAGCCCAAGCCGCTCTAGCTCAGCGCTATGGTTAG

Upstream 100 bases:

>100_bases
ACGGAAACCCCCCTTCCGCGTCCGTCTCCTCGCTTGTCAGTTGAGCAATTGCAGCGATTTCTGCAAAAGTATCTTTAGTG
TTTTTATCGACATCAACGGA

Downstream 100 bases:

>100_bases
GACTTTTCCTGCTTGAAACGTTTATGAGACAGGCAGAGGCGGCCGAGGCAGCCATTCAGGAAGATTGGAGTCAGAGAATT
CAGCCAAAGCGCCTAGGGCA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MGMVKLRTLLAELSTHLWETPPTDHPALDAEITGLTTNSWAVQPGYLFIGMPGTRVDGGSFWKSAIEAGAAAALVSDQVD
RADVGTHCVLALKDMPRACAEVAATFYDQPSQKLKLVGMTGTNGKTTTTHLIEYLLTTAQAPTALFGTLYARWPGHQVTA
AHTTPFAVDLQQQLSDAVAAQCQFGVMEVSSHALAQKRVLGCQFTVAVFSNLTQDHLDYHQDMEDYFAAKALLFSPDYLQ
GRAIINIDDVYGQRLVDQIGPNQCWTYSIEGPADLYTSGLSYETAGVKGQLHTPKGTVEFFSSLVGQFNVSNLLAAVGAV
LELGLTLEQITPALAQFPGVPGRMQQITYSADQDISVIVDYAHTPDSLENLLKAARPFVKGQMACVFGCGGDRDRTKRPL
MGEIAARLSDQAIVTSDNPRTEDPQQILQDVLAGIPMEVKPIVEVDRRVAIQAAIAQAKPGDTILIAGKGHEDYQILGTE
KIHFDDREEAQAALAQRYG

Sequences:

>Translated_499_residues
MGMVKLRTLLAELSTHLWETPPTDHPALDAEITGLTTNSWAVQPGYLFIGMPGTRVDGGSFWKSAIEAGAAAALVSDQVD
RADVGTHCVLALKDMPRACAEVAATFYDQPSQKLKLVGMTGTNGKTTTTHLIEYLLTTAQAPTALFGTLYARWPGHQVTA
AHTTPFAVDLQQQLSDAVAAQCQFGVMEVSSHALAQKRVLGCQFTVAVFSNLTQDHLDYHQDMEDYFAAKALLFSPDYLQ
GRAIINIDDVYGQRLVDQIGPNQCWTYSIEGPADLYTSGLSYETAGVKGQLHTPKGTVEFFSSLVGQFNVSNLLAAVGAV
LELGLTLEQITPALAQFPGVPGRMQQITYSADQDISVIVDYAHTPDSLENLLKAARPFVKGQMACVFGCGGDRDRTKRPL
MGEIAARLSDQAIVTSDNPRTEDPQQILQDVLAGIPMEVKPIVEVDRRVAIQAAIAQAKPGDTILIAGKGHEDYQILGTE
KIHFDDREEAQAALAQRYG
>Mature_498_residues
GMVKLRTLLAELSTHLWETPPTDHPALDAEITGLTTNSWAVQPGYLFIGMPGTRVDGGSFWKSAIEAGAAAALVSDQVDR
ADVGTHCVLALKDMPRACAEVAATFYDQPSQKLKLVGMTGTNGKTTTTHLIEYLLTTAQAPTALFGTLYARWPGHQVTAA
HTTPFAVDLQQQLSDAVAAQCQFGVMEVSSHALAQKRVLGCQFTVAVFSNLTQDHLDYHQDMEDYFAAKALLFSPDYLQG
RAIINIDDVYGQRLVDQIGPNQCWTYSIEGPADLYTSGLSYETAGVKGQLHTPKGTVEFFSSLVGQFNVSNLLAAVGAVL
ELGLTLEQITPALAQFPGVPGRMQQITYSADQDISVIVDYAHTPDSLENLLKAARPFVKGQMACVFGCGGDRDRTKRPLM
GEIAARLSDQAIVTSDNPRTEDPQQILQDVLAGIPMEVKPIVEVDRRVAIQAAIAQAKPGDTILIAGKGHEDYQILGTEK
IHFDDREEAQAALAQRYG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=474, Percent_Identity=41.3502109704641, Blast_Score=287, Evalue=9e-79,
Organism=Escherichia coli, GI1786274, Length=334, Percent_Identity=26.6467065868263, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53979; Mature: 53848

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMVKLRTLLAELSTHLWETPPTDHPALDAEITGLTTNSWAVQPGYLFIGMPGTRVDGGS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEECCCEEEEECCCCCCCCHH
FWKSAIEAGAAAALVSDQVDRADVGTHCVLALKDMPRACAEVAATFYDQPSQKLKLVGMT
HHHHHHHHCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GTNGKTTTTHLIEYLLTTAQAPTALFGTLYARWPGHQVTAAHTTPFAVDLQQQLSDAVAA
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHH
QCQFGVMEVSSHALAQKRVLGCQFTVAVFSNLTQDHLDYHQDMEDYFAAKALLFSPDYLQ
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCC
GRAIINIDDVYGQRLVDQIGPNQCWTYSIEGPADLYTSGLSYETAGVKGQLHTPKGTVEF
CCEEEEEHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCEECCCCCCEEECCCHHHHH
FSSLVGQFNVSNLLAAVGAVLELGLTLEQITPALAQFPGVPGRMQQITYSADQDISVIVD
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHEEECCCCCEEEEEE
YAHTPDSLENLLKAARPFVKGQMACVFGCGGDRDRTKRPLMGEIAARLSDQAIVTSDNPR
ECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEECCCCC
TEDPQQILQDVLAGIPMEVKPIVEVDRRVAIQAAIAQAKPGDTILIAGKGHEDYQILGTE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCE
KIHFDDREEAQAALAQRYG
EECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GMVKLRTLLAELSTHLWETPPTDHPALDAEITGLTTNSWAVQPGYLFIGMPGTRVDGGS
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEECCCEEEEECCCCCCCCHH
FWKSAIEAGAAAALVSDQVDRADVGTHCVLALKDMPRACAEVAATFYDQPSQKLKLVGMT
HHHHHHHHCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GTNGKTTTTHLIEYLLTTAQAPTALFGTLYARWPGHQVTAAHTTPFAVDLQQQLSDAVAA
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHH
QCQFGVMEVSSHALAQKRVLGCQFTVAVFSNLTQDHLDYHQDMEDYFAAKALLFSPDYLQ
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCC
GRAIINIDDVYGQRLVDQIGPNQCWTYSIEGPADLYTSGLSYETAGVKGQLHTPKGTVEF
CCEEEEEHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCEECCCCCCEEECCCHHHHH
FSSLVGQFNVSNLLAAVGAVLELGLTLEQITPALAQFPGVPGRMQQITYSADQDISVIVD
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHEEECCCCCEEEEEE
YAHTPDSLENLLKAARPFVKGQMACVFGCGGDRDRTKRPLMGEIAARLSDQAIVTSDNPR
ECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEECCCCC
TEDPQQILQDVLAGIPMEVKPIVEVDRRVAIQAAIAQAKPGDTILIAGKGHEDYQILGTE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCE
KIHFDDREEAQAALAQRYG
EECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]