Click here to switch to the map view.

The map label for this gene is kefA [C]

Identifier: 158334573

GI number: 158334573

Start: 1381017

End: 1382468

Strand: Reverse

Name: kefA [C]

Synonym: AM1_1400

Alternate gene names: 158334573

Gene position: 1382468-1381017 (Counterclockwise)

Preceding gene: 158334574

Following gene: 158334572

Centisome position: 21.26

GC content: 47.73

Gene sequence:

>1452_bases
ATGACTTCCTCTCTTCTTCAGCAATCCTGGTTCCTCTGGAGTTGCGGCTTGATGGTTGGGTTTCCCTGCCTGATGTTGGT
TTTAAATGAATCGATTGTCTATACCCAGAAACAGGGGAAACCGCTTGTTCACACCTTGAGAATCCTACGAAATTTGGTCT
TGCCGATTTTTGTTGGGTTTATTGTCCTGACCAAAATTGTCGGCTTAGATCCTGAACAAACGGCCATTCGCTTGGTTCAG
ACGTCTTTGTGGGTGTCCTTGATCCATGCGGCGTTGTCCTTTGTAAATGTTTTGCTGTTTGTGGAGGCCGGGGGCCATAC
TTGGCAAGCCCAGGTGCCTAAACTCCTGAGAGATTTAACTCGATTTTTCTTGATATTAATCGGGACGGCCTTTGTGCTCT
CTTTGGTTTGGAAAACGGATTTAGGCGGCCTCCTCACGGCCTTAGGGGTTAGCTCTATTGTTATTGGTTTGGCCCTGCAA
GATACCCTCGGCAACTTGTTTTCAGGAATTGCGCTGCTGTTTGGCCGCCCCTTTGTGATTGGCGATTGGCTCAAGTTTGG
GGACACGGTCGGCAAGGTGATTGAGATCAATTGGCGTGCAGTCCATTTGATTACCCGCAATAAAGAAATGCTGATTGTGC
CCAACTCTGTATTGGCCAAGGAAGTGTTCTATAACTATCGACGCCCCCAATCGTTGCATGGGGAACCGATGGAATTGGGG
TTTTCCTACGATGACCCACCCAATAAGGTTAAGCAGGTGATCCGGCAAGCGGCTCTAGAAACACCAGGTGTCTTATCGGA
TCCAGAACCGATTATCCAAACTAGTGCCTATGGGGATTCATCCATTAATTACTATGTCCGTTTATATTTGCGAGACTATG
ATCGGGTGCCCGAGATCCGCGATGCATTTATGACTCGGGTTTGGTATGCTGCCCAACGCCATGGCTTAACGATTCCATTT
CCCATCCGCACTCTGATTCATGAACGCCCCCCCAAACCTGAGTTGAATGGCGGTACGAATCGGATTATTACGGAGTTGCG
ATCGCTACCCTGTTTCATCACTACCCATACAGAAGTCTTGGAGAAACTGGTGACCAATGCAGACTTTAAATCCTTTGGCA
AAGGCGAGCGTATTATCTATCAAGGACAGGAAGAGGTAAATCTGCACTTTCTGTTGACTGGGCAGGTCCAGATGAACGTC
TTGAGCCAAACCAACCATGACCAAGAAGTGCTGAGGTTATCCAAAGGAGATTTTTTTGGGGCCACAGCTTTACTCTCTAG
AGAACCGAGTTCTAACTCCGTTGTTGCTCTGAGTGATGTGGATGTCATGATTTTAGAAATCGAAGCCATGCAGGTTTTGA
TTGAACGCACCCCCCAACTGGCAAGAGAGATGGATGAAATCATCGAAGCCCGCCGCAAAGCGATTCAAATTGCCCAACAG
ACCTATGCTTAA

Upstream 100 bases:

>100_bases
CCGTGAAGTAGAAATTAGCGGCGATAATAAGCTCCCTGCTTGGTTGCTCAAGATTTAGCACTCTTTTTTTCATCTCCCCT
GACATCTTTTTAGTGCAAGT

Downstream 100 bases:

>100_bases
CCGATGACATCAGGGTTTGATTAAGAATCGGGGTTAGGTCAAAGTTGAAGATTTTGTTAAACTGGTTACTGCGAAAGTGC
TCAAGTTTTTAAAATTTGCC

Product: mechanosensitive ion channel/cyclic nucleotide-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTSSLLQQSWFLWSCGLMVGFPCLMLVLNESIVYTQKQGKPLVHTLRILRNLVLPIFVGFIVLTKIVGLDPEQTAIRLVQ
TSLWVSLIHAALSFVNVLLFVEAGGHTWQAQVPKLLRDLTRFFLILIGTAFVLSLVWKTDLGGLLTALGVSSIVIGLALQ
DTLGNLFSGIALLFGRPFVIGDWLKFGDTVGKVIEINWRAVHLITRNKEMLIVPNSVLAKEVFYNYRRPQSLHGEPMELG
FSYDDPPNKVKQVIRQAALETPGVLSDPEPIIQTSAYGDSSINYYVRLYLRDYDRVPEIRDAFMTRVWYAAQRHGLTIPF
PIRTLIHERPPKPELNGGTNRIITELRSLPCFITTHTEVLEKLVTNADFKSFGKGERIIYQGQEEVNLHFLLTGQVQMNV
LSQTNHDQEVLRLSKGDFFGATALLSREPSSNSVVALSDVDVMILEIEAMQVLIERTPQLAREMDEIIEARRKAIQIAQQ
TYA

Sequences:

>Translated_483_residues
MTSSLLQQSWFLWSCGLMVGFPCLMLVLNESIVYTQKQGKPLVHTLRILRNLVLPIFVGFIVLTKIVGLDPEQTAIRLVQ
TSLWVSLIHAALSFVNVLLFVEAGGHTWQAQVPKLLRDLTRFFLILIGTAFVLSLVWKTDLGGLLTALGVSSIVIGLALQ
DTLGNLFSGIALLFGRPFVIGDWLKFGDTVGKVIEINWRAVHLITRNKEMLIVPNSVLAKEVFYNYRRPQSLHGEPMELG
FSYDDPPNKVKQVIRQAALETPGVLSDPEPIIQTSAYGDSSINYYVRLYLRDYDRVPEIRDAFMTRVWYAAQRHGLTIPF
PIRTLIHERPPKPELNGGTNRIITELRSLPCFITTHTEVLEKLVTNADFKSFGKGERIIYQGQEEVNLHFLLTGQVQMNV
LSQTNHDQEVLRLSKGDFFGATALLSREPSSNSVVALSDVDVMILEIEAMQVLIERTPQLAREMDEIIEARRKAIQIAQQ
TYA
>Mature_482_residues
TSSLLQQSWFLWSCGLMVGFPCLMLVLNESIVYTQKQGKPLVHTLRILRNLVLPIFVGFIVLTKIVGLDPEQTAIRLVQT
SLWVSLIHAALSFVNVLLFVEAGGHTWQAQVPKLLRDLTRFFLILIGTAFVLSLVWKTDLGGLLTALGVSSIVIGLALQD
TLGNLFSGIALLFGRPFVIGDWLKFGDTVGKVIEINWRAVHLITRNKEMLIVPNSVLAKEVFYNYRRPQSLHGEPMELGF
SYDDPPNKVKQVIRQAALETPGVLSDPEPIIQTSAYGDSSINYYVRLYLRDYDRVPEIRDAFMTRVWYAAQRHGLTIPFP
IRTLIHERPPKPELNGGTNRIITELRSLPCFITTHTEVLEKLVTNADFKSFGKGERIIYQGQEEVNLHFLLTGQVQMNVL
SQTNHDQEVLRLSKGDFFGATALLSREPSSNSVVALSDVDVMILEIEAMQVLIERTPQLAREMDEIIEARRKAIQIAQQT
YA

Specific function: Unknown

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=150, Percent_Identity=32.6666666666667, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787591, Length=202, Percent_Identity=29.2079207920792, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789291, Length=213, Percent_Identity=25.3521126760563, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI2367355, Length=206, Percent_Identity=26.6990291262136, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 54424; Mature: 54293

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSLLQQSWFLWSCGLMVGFPCLMLVLNESIVYTQKQGKPLVHTLRILRNLVLPIFVGF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
IVLTKIVGLDPEQTAIRLVQTSLWVSLIHAALSFVNVLLFVEAGGHTWQAQVPKLLRDLT
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
RFFLILIGTAFVLSLVWKTDLGGLLTALGVSSIVIGLALQDTLGNLFSGIALLFGRPFVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
GDWLKFGDTVGKVIEINWRAVHLITRNKEMLIVPNSVLAKEVFYNYRRPQSLHGEPMELG
EHHHHHCCHHCCEEEECEEEEEEEECCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCEEC
FSYDDPPNKVKQVIRQAALETPGVLSDPEPIIQTSAYGDSSINYYVRLYLRDYDRVPEIR
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCCCCCCEEEEEEEECCCCCCCHHH
DAFMTRVWYAAQRHGLTIPFPIRTLIHERPPKPELNGGTNRIITELRSLPCFITTHTEVL
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEECHHHHH
EKLVTNADFKSFGKGERIIYQGQEEVNLHFLLTGQVQMNVLSQTNHDQEVLRLSKGDFFG
HHHHCCCCHHHCCCCCEEEEECCCCCEEEEEEECCEEEHHHHCCCCHHHHHHHHCCCCCC
ATALLSREPSSNSVVALSDVDVMILEIEAMQVLIERTPQLAREMDEIIEARRKAIQIAQQ
HHHHHCCCCCCCCEEEEECCCEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
TYA
HCC
>Mature Secondary Structure 
TSSLLQQSWFLWSCGLMVGFPCLMLVLNESIVYTQKQGKPLVHTLRILRNLVLPIFVGF
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
IVLTKIVGLDPEQTAIRLVQTSLWVSLIHAALSFVNVLLFVEAGGHTWQAQVPKLLRDLT
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
RFFLILIGTAFVLSLVWKTDLGGLLTALGVSSIVIGLALQDTLGNLFSGIALLFGRPFVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
GDWLKFGDTVGKVIEINWRAVHLITRNKEMLIVPNSVLAKEVFYNYRRPQSLHGEPMELG
EHHHHHCCHHCCEEEECEEEEEEEECCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCEEC
FSYDDPPNKVKQVIRQAALETPGVLSDPEPIIQTSAYGDSSINYYVRLYLRDYDRVPEIR
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCCCCCCEEEEEEEECCCCCCCHHH
DAFMTRVWYAAQRHGLTIPFPIRTLIHERPPKPELNGGTNRIITELRSLPCFITTHTEVL
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEECHHHHH
EKLVTNADFKSFGKGERIIYQGQEEVNLHFLLTGQVQMNVLSQTNHDQEVLRLSKGDFFG
HHHHCCCCHHHCCCCCEEEEECCCCCEEEEEEECCEEEHHHHCCCCHHHHHHHHCCCCCC
ATALLSREPSSNSVVALSDVDVMILEIEAMQVLIERTPQLAREMDEIIEARRKAIQIAQQ
HHHHHCCCCCCCCEEEEECCCEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
TYA
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]