Click here to switch to the map view.

The map label for this gene is amyB [H]

Identifier: 158334461

GI number: 158334461

Start: 1274990

End: 1276255

Strand: Direct

Name: amyB [H]

Synonym: AM1_1282

Alternate gene names: 158334461

Gene position: 1274990-1276255 (Clockwise)

Preceding gene: 158334460

Following gene: 158334463

Centisome position: 19.6

GC content: 51.03

Gene sequence:

>1266_bases
ATGCTGCGTGTTTCCCCACGATTTTCTAGAATTGCCCTGACCTGGGGAGTATCCCTCAGCGCCATTGCGCCCATTTTCTC
AGCACTCCCTGCATCGGCCCAAGCCAATTTTTCTGATGTCAGCCTTGGATATTGGGCGCGCCCATTTATCGAAAAACTAG
CTGAGGAGAATGTCATTAAAGGCTTCCCGGACGGGACCTTCAAACCTGATCAGCCTGTCACCCGTGCTCAATTTGCAGCG
ATTGTTCGCCAGGCATTTGATCGTGAGTCTACCCGCCAGTATCGAGGTTTTGCGGATGTCCCCACCAATCATTGGGCCCA
GCCTGCCATTGGTAAGGCTTACAGCACTCGCTTTATGTCAGGTTACCCGGGAAATTTATTTCAACCCAATCAAAGAATCC
CCAAAGTCCAAGCCCTAGTCTCTTTAGCGAGTGGTCTAGAGTTAGAACCCGATGCTCCCACGGATGAGGTACTCGCGACT
TTTCGGGATGCAGCAGATATCCCTGGCTATGCCGACAAAGGTGTCACAGCAGCGACGGAAGCAGGTTTAGTTGTCAACTA
TCCCAATGCCAACTTTTTAAATCCCAATCAGCAAGCCACTCGGGCCGAAATTGCAGCCTTTGTCTACCAAGCCTTGGTCG
ATCAAGGGGATTTAGATCCGATCTCTGACAAAGCGCGGGCCAATAACTACATTGTCAAACTAGACGGCACCGCCTCCCCA
GAGCAACCCAGCACACCAATCAATACGGGGAGTATCATTGCCTCTGGCTCCAAGATTCCTGTCCGATATCCAGGACCCAA
CAAAGTCAATCTGATTGTTGCCCCCGGAGAAACGGTAGACACTAAGCTAGAAGTCGCAGAGGACATTGTCAATACTGCAG
GTACAGTGCTTATTCCCAAAGGCAGTCTGATCGAAGGGACACTGGTACCGGTTAATGTCGGCACAACCCCTGGGACACAG
TTTGTAGCTAAAACCCTCAAAGTAGGGACTCGTAGCTACGACATCAGAGCCAACTCTGATCCCCAGGTAGCGGTTTCTCG
GCAATCCGTCAAACCCAATGATATTAGAGGTGCTATTAGTACAACAGCCGGTCGAACCATTCTGAACTCCGTCCTAGGCA
GTGGGTTTAACTTAGGCAGTTTACTCACTGGTGCCTTGCTAAGTGGGGTAGCCACCAGCCCCTCTGCCCCTAAAGACAGC
GTGATAGTCGTCGATCCAGCCGCATTGGAATTAACCATCCAATCGAGTTTGGATCTAACCACCTAG

Upstream 100 bases:

>100_bases
ATAAAATAAATTCTGCACTTTCTGGTTTCTGGACCGATCCCTTCGATACTCAAAGGGAAGAAAACTCTATTTTTATAAAT
TACTTGGAGGGTCCTTTCGC

Downstream 100 bases:

>100_bases
ACGACAATGTTTCCCCAGCCCTTGAACAATACCTCTACTCTCAAGGACTGGGAAACAGCTTATGGATCTAAAGCCTGCCA
AATCGCCGTCAGAATCGTGT

Product: S-layer region-like protein

Products: NA

Alternate protein names: Alpha-amylase/pullulanase; Pullulanase type II; Alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase; Pullulanase; 1,4-alpha-D-glucan glucanohydrolase; Alpha-dextrin endo-1,6-alpha-glucosidase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MLRVSPRFSRIALTWGVSLSAIAPIFSALPASAQANFSDVSLGYWARPFIEKLAEENVIKGFPDGTFKPDQPVTRAQFAA
IVRQAFDRESTRQYRGFADVPTNHWAQPAIGKAYSTRFMSGYPGNLFQPNQRIPKVQALVSLASGLELEPDAPTDEVLAT
FRDAADIPGYADKGVTAATEAGLVVNYPNANFLNPNQQATRAEIAAFVYQALVDQGDLDPISDKARANNYIVKLDGTASP
EQPSTPINTGSIIASGSKIPVRYPGPNKVNLIVAPGETVDTKLEVAEDIVNTAGTVLIPKGSLIEGTLVPVNVGTTPGTQ
FVAKTLKVGTRSYDIRANSDPQVAVSRQSVKPNDIRGAISTTAGRTILNSVLGSGFNLGSLLTGALLSGVATSPSAPKDS
VIVVDPAALELTIQSSLDLTT

Sequences:

>Translated_421_residues
MLRVSPRFSRIALTWGVSLSAIAPIFSALPASAQANFSDVSLGYWARPFIEKLAEENVIKGFPDGTFKPDQPVTRAQFAA
IVRQAFDRESTRQYRGFADVPTNHWAQPAIGKAYSTRFMSGYPGNLFQPNQRIPKVQALVSLASGLELEPDAPTDEVLAT
FRDAADIPGYADKGVTAATEAGLVVNYPNANFLNPNQQATRAEIAAFVYQALVDQGDLDPISDKARANNYIVKLDGTASP
EQPSTPINTGSIIASGSKIPVRYPGPNKVNLIVAPGETVDTKLEVAEDIVNTAGTVLIPKGSLIEGTLVPVNVGTTPGTQ
FVAKTLKVGTRSYDIRANSDPQVAVSRQSVKPNDIRGAISTTAGRTILNSVLGSGFNLGSLLTGALLSGVATSPSAPKDS
VIVVDPAALELTIQSSLDLTT
>Mature_421_residues
MLRVSPRFSRIALTWGVSLSAIAPIFSALPASAQANFSDVSLGYWARPFIEKLAEENVIKGFPDGTFKPDQPVTRAQFAA
IVRQAFDRESTRQYRGFADVPTNHWAQPAIGKAYSTRFMSGYPGNLFQPNQRIPKVQALVSLASGLELEPDAPTDEVLAT
FRDAADIPGYADKGVTAATEAGLVVNYPNANFLNPNQQATRAEIAAFVYQALVDQGDLDPISDKARANNYIVKLDGTASP
EQPSTPINTGSIIASGSKIPVRYPGPNKVNLIVAPGETVDTKLEVAEDIVNTAGTVLIPKGSLIEGTLVPVNVGTTPGTQ
FVAKTLKVGTRSYDIRANSDPQVAVSRQSVKPNDIRGAISTTAGRTILNSVLGSGFNLGSLLTGALLSGVATSPSAPKDS
VIVVDPAALELTIQSSLDLTT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted, cell wall. Note=It C-terminus may serve as an S-layer anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013784
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR002044
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR001119 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N; PF00395 SLH [H]

EC number: =3.2.1.1; =3.2.1.41 [H]

Molecular weight: Translated: 44506; Mature: 44506

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS01072 SLH_DOMAIN ; PS00359 RIBOSOMAL_L11

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVSPRFSRIALTWGVSLSAIAPIFSALPASAQANFSDVSLGYWARPFIEKLAEENVIK
CCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GFPDGTFKPDQPVTRAQFAAIVRQAFDRESTRQYRGFADVPTNHWAQPAIGKAYSTRFMS
CCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHC
GYPGNLFQPNQRIPKVQALVSLASGLELEPDAPTDEVLATFRDAADIPGYADKGVTAATE
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEECC
AGLVVNYPNANFLNPNQQATRAEIAAFVYQALVDQGDLDPISDKARANNYIVKLDGTASP
CCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEECCCCCC
EQPSTPINTGSIIASGSKIPVRYPGPNKVNLIVAPGETVDTKLEVAEDIVNTAGTVLIPK
CCCCCCCCCCCEEECCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEECC
GSLIEGTLVPVNVGTTPGTQFVAKTLKVGTRSYDIRANSDPQVAVSRQSVKPNDIRGAIS
CCEEECEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCHHHHHHH
TTAGRTILNSVLGSGFNLGSLLTGALLSGVATSPSAPKDSVIVVDPAALELTIQSSLDLT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEEECCCCCC
T
C
>Mature Secondary Structure
MLRVSPRFSRIALTWGVSLSAIAPIFSALPASAQANFSDVSLGYWARPFIEKLAEENVIK
CCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GFPDGTFKPDQPVTRAQFAAIVRQAFDRESTRQYRGFADVPTNHWAQPAIGKAYSTRFMS
CCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHC
GYPGNLFQPNQRIPKVQALVSLASGLELEPDAPTDEVLATFRDAADIPGYADKGVTAATE
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEECC
AGLVVNYPNANFLNPNQQATRAEIAAFVYQALVDQGDLDPISDKARANNYIVKLDGTASP
CCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEECCCCCC
EQPSTPINTGSIIASGSKIPVRYPGPNKVNLIVAPGETVDTKLEVAEDIVNTAGTVLIPK
CCCCCCCCCCCEEECCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEECC
GSLIEGTLVPVNVGTTPGTQFVAKTLKVGTRSYDIRANSDPQVAVSRQSVKPNDIRGAIS
CCEEECEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCHHHHHHH
TTAGRTILNSVLGSGFNLGSLLTGALLSGVATSPSAPKDSVIVVDPAALELTIQSSLDLT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEEECCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8195085 [H]