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The map label for this gene is rpoA [H]

Identifier: 158334432

GI number: 158334432

Start: 1236464

End: 1237405

Strand: Reverse

Name: rpoA [H]

Synonym: AM1_1253

Alternate gene names: 158334432

Gene position: 1237405-1236464 (Counterclockwise)

Preceding gene: 158334433

Following gene: 158334431

Centisome position: 19.03

GC content: 47.77

Gene sequence:

>942_bases
GTGGCGCAATTTCAGGTTGAATGTCTTGAAACTACAGTTGAAGAGGATTTTAGCCAGTATAGCAGGTTTATTCTGGAGCC
CTTAGATCGGGGGCAAGGCACGACAGTTGGAAATGCTCTTAGACGCGTTTTACTTGCAAATCTGAGAGGAACTGCGGTCA
CGGCTGTTCGTATTGCTGGTGTGAACCATGAATTTGCTACCATTCCTGGGGTTCGTGAGGATGTTCTCGACATTCTGCTG
AATATGAAGCAGGTTGTCTTGAGAAGCCATACCAACGATCCGCAGATTGGTCGCTTATTTGTCCAAGGTCCAGCGACGGT
CACGACCGATGACTTTGAATTACCCACTGAAGTTGAGATTGTAAATCCAACCCAGTACGTCGCGACTCTGTCTTCGGATG
CCACCCTAGAGATGGAGTTTAGAATCGAGCAGGGTAAGGGATACCATGCGGTAGGTAAAAACCGAGATGAAGCCGCAGCC
CTTGACTTTTTAGAGATTGATGCGATTTATATGCCCGTCCGACGGGTCAACTACGAAGTAGAAGACGCTCGGGTCGGTGG
TGCCTTAGAAAAGGATCGCCTGATTCTGGATATCTGGACCAATGGCAATCTGACGCCCCAGGAATCCTTAAGTCAGGCTG
CTACCATTTTGGTTGATCTGTTCAACCCCCTCAAAGATGCCACCTTTAACGTGGTTGAGGATATTGAGCTGGAAGAAGAA
GACGAGACCAATCAAATCCCCATTGAAGGTCTAAACCTCTCTGTCCGGGCCTATAACTGCCTCAAGCGGGCTCAAATCAA
TAGCGTGGCGGATCTTCTGGAATATTCCCAAGAAGACCTCCTCGAAATCAAGAATTTTGGAGCCAAGTCTGCGGATGAAG
TGATCGCAGCTCTCCAAGATCATCTTGGTATTACTATGCCGACTGAAAAATCGAAAAGTTAA

Upstream 100 bases:

>100_bases
ACCTCAAGCTAAAGCTAACTTTTAAAAACTTTTTTCTTCACTCCACAGGTTCCAAAGAACCGACCTACATTTCGAGCACA
GCAACAAGGGAGGGATTTTC

Downstream 100 bases:

>100_bases
GGTTAATTTCCATGCGTCATCGTTGTCGAGTTCATAAGTTAGGCAAACCTGCCGACCAGCGCAAGGCTCTCCTCAGATCC
TTGTCTACTCAGCTGATTCG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MAQFQVECLETTVEEDFSQYSRFILEPLDRGQGTTVGNALRRVLLANLRGTAVTAVRIAGVNHEFATIPGVREDVLDILL
NMKQVVLRSHTNDPQIGRLFVQGPATVTTDDFELPTEVEIVNPTQYVATLSSDATLEMEFRIEQGKGYHAVGKNRDEAAA
LDFLEIDAIYMPVRRVNYEVEDARVGGALEKDRLILDIWTNGNLTPQESLSQAATILVDLFNPLKDATFNVVEDIELEEE
DETNQIPIEGLNLSVRAYNCLKRAQINSVADLLEYSQEDLLEIKNFGAKSADEVIAALQDHLGITMPTEKSKS

Sequences:

>Translated_313_residues
MAQFQVECLETTVEEDFSQYSRFILEPLDRGQGTTVGNALRRVLLANLRGTAVTAVRIAGVNHEFATIPGVREDVLDILL
NMKQVVLRSHTNDPQIGRLFVQGPATVTTDDFELPTEVEIVNPTQYVATLSSDATLEMEFRIEQGKGYHAVGKNRDEAAA
LDFLEIDAIYMPVRRVNYEVEDARVGGALEKDRLILDIWTNGNLTPQESLSQAATILVDLFNPLKDATFNVVEDIELEEE
DETNQIPIEGLNLSVRAYNCLKRAQINSVADLLEYSQEDLLEIKNFGAKSADEVIAALQDHLGITMPTEKSKS
>Mature_312_residues
AQFQVECLETTVEEDFSQYSRFILEPLDRGQGTTVGNALRRVLLANLRGTAVTAVRIAGVNHEFATIPGVREDVLDILLN
MKQVVLRSHTNDPQIGRLFVQGPATVTTDDFELPTEVEIVNPTQYVATLSSDATLEMEFRIEQGKGYHAVGKNRDEAAAL
DFLEIDAIYMPVRRVNYEVEDARVGGALEKDRLILDIWTNGNLTPQESLSQAATILVDLFNPLKDATFNVVEDIELEEED
ETNQIPIEGLNLSVRAYNCLKRAQINSVADLLEYSQEDLLEIKNFGAKSADEVIAALQDHLGITMPTEKSKS

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:
GO:0003677: DNA-directed RNA polymerase subunit alpha
GO:0003899: DNA-directed RNA polymerase subunit alpha
GO:0006281: DNA-directed RNA polymerase subunit alpha
GO:0006351: DNA-directed RNA polymerase subunit alpha
GO:0016740: DNA-directed RNA polymerase subunit alpha
GO:0016779: DNA-directed RNA polymerase subunit alpha
GO:0046983: DNA-directed RNA polymerase subunit alpha

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=292, Percent_Identity=37.6712328767123, Blast_Score=184, Evalue=8e-48,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR003583
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 34811; Mature: 34679

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFQVECLETTVEEDFSQYSRFILEPLDRGQGTTVGNALRRVLLANLRGTAVTAVRIAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEC
VNHEFATIPGVREDVLDILLNMKQVVLRSHTNDPQIGRLFVQGPATVTTDDFELPTEVEI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEECCCCCCCCEEEE
VNPTQYVATLSSDATLEMEFRIEQGKGYHAVGKNRDEAAALDFLEIDAIYMPVRRVNYEV
ECCHHEEEEECCCCEEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHEECHHHHCCEE
EDARVGGALEKDRLILDIWTNGNLTPQESLSQAATILVDLFNPLKDATFNVVEDIELEEE
CHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCC
DETNQIPIEGLNLSVRAYNCLKRAQINSVADLLEYSQEDLLEIKNFGAKSADEVIAALQD
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHH
HLGITMPTEKSKS
HCCCCCCCCCCCC
>Mature Secondary Structure 
AQFQVECLETTVEEDFSQYSRFILEPLDRGQGTTVGNALRRVLLANLRGTAVTAVRIAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEC
VNHEFATIPGVREDVLDILLNMKQVVLRSHTNDPQIGRLFVQGPATVTTDDFELPTEVEI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEECCCCCCCCEEEE
VNPTQYVATLSSDATLEMEFRIEQGKGYHAVGKNRDEAAALDFLEIDAIYMPVRRVNYEV
ECCHHEEEEECCCCEEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHEECHHHHCCEE
EDARVGGALEKDRLILDIWTNGNLTPQESLSQAATILVDLFNPLKDATFNVVEDIELEEE
CHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCC
DETNQIPIEGLNLSVRAYNCLKRAQINSVADLLEYSQEDLLEIKNFGAKSADEVIAALQD
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHH
HLGITMPTEKSKS
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA