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The map label for this gene is truA [H]

Identifier: 158334430

GI number: 158334430

Start: 1235204

End: 1236082

Strand: Reverse

Name: truA [H]

Synonym: AM1_1251

Alternate gene names: 158334430

Gene position: 1236082-1235204 (Counterclockwise)

Preceding gene: 158334431

Following gene: 158334429

Centisome position: 19.01

GC content: 54.27

Gene sequence:

>879_bases
ATGCCATTGCCATCCCCAACATCCCAACGAGTTGCCCTGGTTATTCAGTACCTGGGCACTCATTTTCATGGCTGGCAGCG
GCAACCTCAGCATCGAAGTGTGCAGGCGGATATCGAGGATACCTTAGCGGAAATTGTTCAGCATCCGGTCACCCTTCATG
GTGCAGGCAGGACCGATTCGGGCGTCCATGCAGCGGCTCAAGTGGCCCATTTTGATGTAGAGTCTCCGATTCCAGCCCAT
CGGTGGGCAAGTGTTCTCAATGCTCGGTTGCCCCAGGACATTTTGATTCGTGGATCAGCCCTAGTCCCTTCGGACTGGCA
TGCTCGCTTTTCTGCAACGTGGCGACGATATCGGTACACTTTGTATACTGATCCTTGCCCCAATCTGTTTTTGCGCCCCT
ATAGCTGGCATTATTATTATCAGCCCTTAGATGTGCAGCGAATGCAGGCGGCTCTTGACCCGCTGATTGGTCGTCATCAC
CTGGCTGCCTTTCACCGGGCTGGCTCCAGTCGTCCCCACTCCTGGGTAGATGTGCATGAAGCGACTTGTGTGCAGCGACC
AACGGTCGAAGCGAAAGACAGTAGCCCTTTGATTCAGATTGAAGTCCAGGCGAGCGGTTTCCTGTACGGGATGATGCGTC
TGCTCGTCGGCTTATTGGTTCAGGTGGGACGGGGATTACGCTCTCCCGATAGCTTTACGGAGTTGTGGCAAGCGGAGCGG
CGGGATCAGGTGAAATATTCTGCCCCGGCCAAAGGTCTTTGCCTTTTGCGGGTGGGATATCCTGACTTTCCCTTTTCCCC
TGATGTTTGGTTTGATACCTTTCCCCATCTGATGATGTCGAGCGTGCCCTCTGAATATTGCTGGAACCCAGCGTCCTAA

Upstream 100 bases:

>100_bases
AACGGTGGCTACACTCGTATTATTCGCACCATCCGTCGGCGTGGCGATAATGCTGAAATGGCGATTATCGAACTTACTTA
AGGACTAAACCGCCTCGGTC

Downstream 100 bases:

>100_bases
CCCCTCTTCCTGTTTTGAAATAGATAACTCTCATGGAAAAAACCTACTTACCCACCGTATCGACCGCTGACAAGAAGTGG
TACGTCGTTGACGCCGAAAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MPLPSPTSQRVALVIQYLGTHFHGWQRQPQHRSVQADIEDTLAEIVQHPVTLHGAGRTDSGVHAAAQVAHFDVESPIPAH
RWASVLNARLPQDILIRGSALVPSDWHARFSATWRRYRYTLYTDPCPNLFLRPYSWHYYYQPLDVQRMQAALDPLIGRHH
LAAFHRAGSSRPHSWVDVHEATCVQRPTVEAKDSSPLIQIEVQASGFLYGMMRLLVGLLVQVGRGLRSPDSFTELWQAER
RDQVKYSAPAKGLCLLRVGYPDFPFSPDVWFDTFPHLMMSSVPSEYCWNPAS

Sequences:

>Translated_292_residues
MPLPSPTSQRVALVIQYLGTHFHGWQRQPQHRSVQADIEDTLAEIVQHPVTLHGAGRTDSGVHAAAQVAHFDVESPIPAH
RWASVLNARLPQDILIRGSALVPSDWHARFSATWRRYRYTLYTDPCPNLFLRPYSWHYYYQPLDVQRMQAALDPLIGRHH
LAAFHRAGSSRPHSWVDVHEATCVQRPTVEAKDSSPLIQIEVQASGFLYGMMRLLVGLLVQVGRGLRSPDSFTELWQAER
RDQVKYSAPAKGLCLLRVGYPDFPFSPDVWFDTFPHLMMSSVPSEYCWNPAS
>Mature_291_residues
PLPSPTSQRVALVIQYLGTHFHGWQRQPQHRSVQADIEDTLAEIVQHPVTLHGAGRTDSGVHAAAQVAHFDVESPIPAHR
WASVLNARLPQDILIRGSALVPSDWHARFSATWRRYRYTLYTDPCPNLFLRPYSWHYYYQPLDVQRMQAALDPLIGRHHL
AAFHRAGSSRPHSWVDVHEATCVQRPTVEAKDSSPLIQIEVQASGFLYGMMRLLVGLLVQVGRGLRSPDSFTELWQAERR
DQVKYSAPAKGLCLLRVGYPDFPFSPDVWFDTFPHLMMSSVPSEYCWNPAS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:
GO:0001522: Pseudouridine synthase
GO:0003723: Pseudouridine synthase
GO:0008033: Pseudouridine synthase
GO:0009451: Pseudouridine synthase
GO:0009982: Pseudouridine synthase
GO:0016853: Pseudouridine synthase
GO:0031119: Pseudouridine synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=293, Percent_Identity=30.7167235494881, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI199559785, Length=266, Percent_Identity=26.6917293233083, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788657, Length=261, Percent_Identity=36.0153256704981, Blast_Score=165, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71985046, Length=312, Percent_Identity=24.0384615384615, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=29.7029702970297, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 33196; Mature: 33065

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPSPTSQRVALVIQYLGTHFHGWQRQPQHRSVQADIEDTLAEIVQHPVTLHGAGRTDS
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCC
GVHAAAQVAHFDVESPIPAHRWASVLNARLPQDILIRGSALVPSDWHARFSATWRRYRYT
CHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHEEECCCCCCCCHHHHHHHHHHHEEEE
LYTDPCPNLFLRPYSWHYYYQPLDVQRMQAALDPLIGRHHLAAFHRAGSSRPHSWVDVHE
EEECCCCCCEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
ATCVQRPTVEAKDSSPLIQIEVQASGFLYGMMRLLVGLLVQVGRGLRSPDSFTELWQAER
HHHHCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
RDQVKYSAPAKGLCLLRVGYPDFPFSPDVWFDTFPHLMMSSVPSEYCWNPAS
HCCCEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCC
>Mature Secondary Structure 
PLPSPTSQRVALVIQYLGTHFHGWQRQPQHRSVQADIEDTLAEIVQHPVTLHGAGRTDS
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCC
GVHAAAQVAHFDVESPIPAHRWASVLNARLPQDILIRGSALVPSDWHARFSATWRRYRYT
CHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHEEECCCCCCCCHHHHHHHHHHHEEEE
LYTDPCPNLFLRPYSWHYYYQPLDVQRMQAALDPLIGRHHLAAFHRAGSSRPHSWVDVHE
EEECCCCCCEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
ATCVQRPTVEAKDSSPLIQIEVQASGFLYGMMRLLVGLLVQVGRGLRSPDSFTELWQAER
HHHHCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
RDQVKYSAPAKGLCLLRVGYPDFPFSPDVWFDTFPHLMMSSVPSEYCWNPAS
HCCCEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]