Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 158334366

GI number: 158334366

Start: 1167699

End: 1169726

Strand: Reverse

Name: ligA

Synonym: AM1_1187

Alternate gene names: 158334366

Gene position: 1169726-1167699 (Counterclockwise)

Preceding gene: 158334367

Following gene: 158334363

Centisome position: 17.99

GC content: 52.12

Gene sequence:

>2028_bases
GTGAACACGGTATCCTCTTCAGATCAAGCGCGGGTGCAAGAGCTGCGACAGTTGCTGCAAAAAGCGAGCTATGCCTACTA
TGGGCTGGATGCTCCCCTGATGGAAGATGCCGTCTATGACCAGCTTTACCATGAGCTGGTAGATTTGGAGGCCCAGTATC
CCCAATTGATTACACCGGATAGTCCCACCCAGCGGGTGGGGGAACGGCCTGCTACACAGTTTGTTTCGGTTCAGCATCGG
ATTCCCCTTTATAGTTTGGAAAACGCTTTTGACCGGGGGGATATGGACACCTGGGATGAGCGCTGGCATAAATTAGTCCC
CAACCTCAGCCAAGAGCCGGGGTATGTGACCGAACTCAAAATCGATGGATCGGCGTTGGCCTTGACCTATGAAAACGGCT
TATTGGTGCGGGGCACCACCCGTGGCGATGGCACCAAGGGAGAAGAGATTACCCAAAATGTAAGAACGATCCGCTCTATT
CCCCTGCGTTTGAATATTGAACATCCCCCCGAATGGCTAGAGGTAAGGGGGGAAGCGTTTTTAGGATTACAGGTATTTGA
CCAAATTAACCGTGATCGCATTCAGGCAGGAGAAGCCGAGTTTGCCAATCCTCGCAATGCCGCCGCTGGCACCCTCCGTC
AGCTTGACTCCAAAGTAGTGGCCGAGCGCAAGCTGGATTTCTTCGCCTATACGATCCATATTTCTGGCCCCACGGATGAT
TTGGCCCTTCCCCAAACCCAATGGCAAGCTTTAGAAACCCTGAAGCAGCTGGGCTTTCGGGTTAATCCTAATCGACGCCA
GTGTGAAAGTCTGGCAGAGGTGCAGACCTACTATGATGACTGGTCCCTAAAACGTCTCGATCTACCCTATTTGACCGATG
GAGTGGTGATTAAGCTGGATAACTTAGGGGTACAGGACGAACTGGGGTTTACCCAGAAGTTTCCTCGCTGGGCGATTGCC
TGGAAGTATCCACCGGAAGAAGCGGCCACTCGCATCGAGCAAATTACAGTCAATGTGGGTCGGACTGGCGCCCTCACTCC
CGTGGCTGAATTTAAACCCGTCCAGTTAGCGGGGACAACAGTCTCACGAGCGACGCTGCATAATCGAGATCGCATCTCGG
AACTCGACATCCATATTGGCGATACGGTGGTGGTCCGTAAAGCCGGAGAAATTATTCCTGAAGTGTTGCGGGTTCTGCCT
GAGTTACGACCTGCGGGGGCTCAGCCCTACCAGATGCCCACGGCCTGCCCGAGCTGTGGACAGCCTGCCGTGCAGCTTGA
AACGGAAGCAGTAACCCGCTGTGTGAATGCCTCTTGCCCTGCCATCTTGCGCGGATCGCTGATTCACTGGGTCAGCCGAG
GGGCTTTAGATATTGATGGGTTGGGAGAAAAGATTGTCGGTCAGTTGACGGATTCCCAAATGGTGCAGTCCGTGGCTGAT
TTATATGAACTGAATGAAGACAAGCTGATGGACTTGGATCGGATGGGGACGAAACTAGCTCGGAAAATTGTGAGTGCGAT
CGCAACCTCCAAACAACAACCCTGGCATCGAGTCCTGTATGGATTGGGAATTCGGCATGTAGGCAGTGTCAACGCTCAGT
TACTGACCGAAAACTATCCAACGGTAGACGCATTAATGGCCGTGGACGGCGATAAGATCGCAACCATCCACGGGATTGGC
CCTGAAATTGCCCAATCTGTTTATGAATGGTTTCAAACCCCCACGAACCAAACTCTGATTCAGCGCCTACAATCTGCGGG
GCTGCAGTTTGAAGCCATCGTATCTGAATCCACTCAGCCTCAAACCTTATCGGGAAAAACCTTTGTGATTACCGGAACAT
TACCGACCTTGAAACGCGATCAGGCCAAGCAGATGATCCAGGATGCAGGGGGCAAAGTCACCGGATCCGTGAGTAAAAAT
ACATCCTATGTGGTGGTCGGTGCAGATGCTGGCTCTAAACTTACAAAAGCACAATCATTAGGAATTAACCAACTATCGGA
AGCCGATCTCCTGGCACTATTACAGTAG

Upstream 100 bases:

>100_bases
TTTTTTTAGCTCTAAACCAGAGTGGGATATACCCTAGCTTCTGGATATCTCTAACCGAGATGAAATTGCCAACCTAATAC
TTTGGGAGATTTTGCGGACT

Downstream 100 bases:

>100_bases
AAATTGAGACAGAACAGGCTTATCCTCAAATCCCAGATGGCTCTGTGGGTAATGTATCAATTGGAGTCTGCTTGACCTAA
ATAATCTGTAAGTATCCCTT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 675; Mature: 675

Protein sequence:

>675_residues
MNTVSSSDQARVQELRQLLQKASYAYYGLDAPLMEDAVYDQLYHELVDLEAQYPQLITPDSPTQRVGERPATQFVSVQHR
IPLYSLENAFDRGDMDTWDERWHKLVPNLSQEPGYVTELKIDGSALALTYENGLLVRGTTRGDGTKGEEITQNVRTIRSI
PLRLNIEHPPEWLEVRGEAFLGLQVFDQINRDRIQAGEAEFANPRNAAAGTLRQLDSKVVAERKLDFFAYTIHISGPTDD
LALPQTQWQALETLKQLGFRVNPNRRQCESLAEVQTYYDDWSLKRLDLPYLTDGVVIKLDNLGVQDELGFTQKFPRWAIA
WKYPPEEAATRIEQITVNVGRTGALTPVAEFKPVQLAGTTVSRATLHNRDRISELDIHIGDTVVVRKAGEIIPEVLRVLP
ELRPAGAQPYQMPTACPSCGQPAVQLETEAVTRCVNASCPAILRGSLIHWVSRGALDIDGLGEKIVGQLTDSQMVQSVAD
LYELNEDKLMDLDRMGTKLARKIVSAIATSKQQPWHRVLYGLGIRHVGSVNAQLLTENYPTVDALMAVDGDKIATIHGIG
PEIAQSVYEWFQTPTNQTLIQRLQSAGLQFEAIVSESTQPQTLSGKTFVITGTLPTLKRDQAKQMIQDAGGKVTGSVSKN
TSYVVVGADAGSKLTKAQSLGINQLSEADLLALLQ

Sequences:

>Translated_675_residues
MNTVSSSDQARVQELRQLLQKASYAYYGLDAPLMEDAVYDQLYHELVDLEAQYPQLITPDSPTQRVGERPATQFVSVQHR
IPLYSLENAFDRGDMDTWDERWHKLVPNLSQEPGYVTELKIDGSALALTYENGLLVRGTTRGDGTKGEEITQNVRTIRSI
PLRLNIEHPPEWLEVRGEAFLGLQVFDQINRDRIQAGEAEFANPRNAAAGTLRQLDSKVVAERKLDFFAYTIHISGPTDD
LALPQTQWQALETLKQLGFRVNPNRRQCESLAEVQTYYDDWSLKRLDLPYLTDGVVIKLDNLGVQDELGFTQKFPRWAIA
WKYPPEEAATRIEQITVNVGRTGALTPVAEFKPVQLAGTTVSRATLHNRDRISELDIHIGDTVVVRKAGEIIPEVLRVLP
ELRPAGAQPYQMPTACPSCGQPAVQLETEAVTRCVNASCPAILRGSLIHWVSRGALDIDGLGEKIVGQLTDSQMVQSVAD
LYELNEDKLMDLDRMGTKLARKIVSAIATSKQQPWHRVLYGLGIRHVGSVNAQLLTENYPTVDALMAVDGDKIATIHGIG
PEIAQSVYEWFQTPTNQTLIQRLQSAGLQFEAIVSESTQPQTLSGKTFVITGTLPTLKRDQAKQMIQDAGGKVTGSVSKN
TSYVVVGADAGSKLTKAQSLGINQLSEADLLALLQ
>Mature_675_residues
MNTVSSSDQARVQELRQLLQKASYAYYGLDAPLMEDAVYDQLYHELVDLEAQYPQLITPDSPTQRVGERPATQFVSVQHR
IPLYSLENAFDRGDMDTWDERWHKLVPNLSQEPGYVTELKIDGSALALTYENGLLVRGTTRGDGTKGEEITQNVRTIRSI
PLRLNIEHPPEWLEVRGEAFLGLQVFDQINRDRIQAGEAEFANPRNAAAGTLRQLDSKVVAERKLDFFAYTIHISGPTDD
LALPQTQWQALETLKQLGFRVNPNRRQCESLAEVQTYYDDWSLKRLDLPYLTDGVVIKLDNLGVQDELGFTQKFPRWAIA
WKYPPEEAATRIEQITVNVGRTGALTPVAEFKPVQLAGTTVSRATLHNRDRISELDIHIGDTVVVRKAGEIIPEVLRVLP
ELRPAGAQPYQMPTACPSCGQPAVQLETEAVTRCVNASCPAILRGSLIHWVSRGALDIDGLGEKIVGQLTDSQMVQSVAD
LYELNEDKLMDLDRMGTKLARKIVSAIATSKQQPWHRVLYGLGIRHVGSVNAQLLTENYPTVDALMAVDGDKIATIHGIG
PEIAQSVYEWFQTPTNQTLIQRLQSAGLQFEAIVSESTQPQTLSGKTFVITGTLPTLKRDQAKQMIQDAGGKVTGSVSKN
TSYVVVGADAGSKLTKAQSLGINQLSEADLLALLQ

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:
GO:0003677: DNA ligase
GO:0003911: DNA ligase
GO:0005622: DNA ligase
GO:0006260: DNA ligase
GO:0006281: DNA ligase
GO:0006974: DNA ligase
GO:0016874: DNA ligase
GO:0046872: DNA ligase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=74, Percent_Identity=51.3513513513513, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=43.5171385991058, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI87082305, Length=523, Percent_Identity=24.0917782026769, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ACAM1 (B0C308)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515538.1
- ProteinModelPortal:   B0C308
- GeneID:   5680010
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1187
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   AMAR329726:AM1_1187-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74772; Mature: 74772

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 120-120 BINDING 118-118 BINDING 141-141 BINDING 178-178 BINDING 298-298 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVSSSDQARVQELRQLLQKASYAYYGLDAPLMEDAVYDQLYHELVDLEAQYPQLITPD
CCCCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SPTQRVGERPATQFVSVQHRIPLYSLENAFDRGDMDTWDERWHKLVPNLSQEPGYVTELK
CHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
IDGSALALTYENGLLVRGTTRGDGTKGEEITQNVRTIRSIPLRLNIEHPPEWLEVRGEAF
ECCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCCEE
LGLQVFDQINRDRIQAGEAEFANPRNAAAGTLRQLDSKVVAERKLDFFAYTIHISGPTDD
EHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCC
LALPQTQWQALETLKQLGFRVNPNRRQCESLAEVQTYYDDWSLKRLDLPYLTDGVVIKLD
CCCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCCCEEEECCCEECCCEEEEEC
NLGVQDELGFTQKFPRWAIAWKYPPEEAATRIEQITVNVGRTGALTPVAEFKPVQLAGTT
CCCCCHHCCCHHCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCCCCHHCCCCEEEECCC
VSRATLHNRDRISELDIHIGDTVVVRKAGEIIPEVLRVLPELRPAGAQPYQMPTACPSCG
HHHHHHCCCCCCCEEEEEECCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
QPAVQLETEAVTRCVNASCPAILRGSLIHWVSRGALDIDGLGEKIVGQLTDSQMVQSVAD
CCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHH
LYELNEDKLMDLDRMGTKLARKIVSAIATSKQQPWHRVLYGLGIRHVGSVNAQLLTENYP
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCCCCCEEEECCCC
TVDALMAVDGDKIATIHGIGPEIAQSVYEWFQTPTNQTLIQRLQSAGLQFEAIVSESTQP
CHHHEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCC
QTLSGKTFVITGTLPTLKRDQAKQMIQDAGGKVTGSVSKNTSYVVVGADAGSKLTKAQSL
CEECCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHHHHHHHC
GINQLSEADLLALLQ
CCCCCCHHHHHHHCC
>Mature Secondary Structure
MNTVSSSDQARVQELRQLLQKASYAYYGLDAPLMEDAVYDQLYHELVDLEAQYPQLITPD
CCCCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SPTQRVGERPATQFVSVQHRIPLYSLENAFDRGDMDTWDERWHKLVPNLSQEPGYVTELK
CHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
IDGSALALTYENGLLVRGTTRGDGTKGEEITQNVRTIRSIPLRLNIEHPPEWLEVRGEAF
ECCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCCEE
LGLQVFDQINRDRIQAGEAEFANPRNAAAGTLRQLDSKVVAERKLDFFAYTIHISGPTDD
EHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCC
LALPQTQWQALETLKQLGFRVNPNRRQCESLAEVQTYYDDWSLKRLDLPYLTDGVVIKLD
CCCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCCCEEEECCCEECCCEEEEEC
NLGVQDELGFTQKFPRWAIAWKYPPEEAATRIEQITVNVGRTGALTPVAEFKPVQLAGTT
CCCCCHHCCCHHCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCCCCHHCCCCEEEECCC
VSRATLHNRDRISELDIHIGDTVVVRKAGEIIPEVLRVLPELRPAGAQPYQMPTACPSCG
HHHHHHCCCCCCCEEEEEECCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
QPAVQLETEAVTRCVNASCPAILRGSLIHWVSRGALDIDGLGEKIVGQLTDSQMVQSVAD
CCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHH
LYELNEDKLMDLDRMGTKLARKIVSAIATSKQQPWHRVLYGLGIRHVGSVNAQLLTENYP
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCCCCCEEEECCCC
TVDALMAVDGDKIATIHGIGPEIAQSVYEWFQTPTNQTLIQRLQSAGLQFEAIVSESTQP
CHHHEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCC
QTLSGKTFVITGTLPTLKRDQAKQMIQDAGGKVTGSVSKNTSYVVVGADAGSKLTKAQSL
CEECCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHHHHHHHC
GINQLSEADLLALLQ
CCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA