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The map label for this gene is yfcG [H]

Identifier: 158334361

GI number: 158334361

Start: 1147561

End: 1148265

Strand: Direct

Name: yfcG [H]

Synonym: AM1_1182

Alternate gene names: 158334361

Gene position: 1147561-1148265 (Clockwise)

Preceding gene: 158334360

Following gene: 158334364

Centisome position: 17.64

GC content: 45.39

Gene sequence:

>705_bases
ATGATTGAGCTATATTATTGGCCCACTCCCAACGGACATAAAATAACCCTATTCCTAGAGGAAGCCGGGCTAGAATACGA
GATTAAGCCCATCAACATTGGGGCTGGGGATCAGTTTCAGACAGATTTTCTCAAAATCTCTCCCAATAATCGGATTCCTG
CCATCATTGACCAAGCCCCTGCGGATGGCGGTGAACCTGTTTCAGTCTTTGAATCTGGCGCTATCTTGTTGTACTTAGCA
GAAAAAACCAGAAAATTCCTGCCGAACGATATACGGCAACGCAATATTGTTCAGGAATGGCTATTTTGGCAAGTAGGGGG
ATTAGGGCCAATGGCTGGGCAAAACCATCACTTTTCTCAATATGCACCAGAAAAGCTGCCCTATGCCATTACCCGATATG
TGAATGAAACGAATCGATTATATGGCGTTTTAAATCAGCATCTCCAAGGGAAAGACTTTATTGCGGGTGACTACTCCATT
GCCGACATGGCCTGTTATCCATGGATTGCGCCCTACAAATGGCAAGGTCAACAGTTAGAGGACTTTCCAGAGATTAACCG
CTGGTTTCAGCAGATTGAACAGAGACCTGCTACCGTCACCGCTTATGAAAAAGGCAAGCAGATCAGCCAATCAGCCCAAT
TGACCGCCGAAAAGAGAAAAGTCTTATTTGGCCAAACGGCAAAAACTCAATCGTCGCAAGTTTGA

Upstream 100 bases:

>100_bases
AGAAGAATAAGTTAGCATCTGTACATCATCACTGCCCTGATCTTGAAAAGAGCAGGCTATATCCCTCACTCCCCTCGCCA
CTGTTCTACCTATTTTCACC

Downstream 100 bases:

>100_bases
GGTGATAGAGGCAAGGCAATGATTTGGGCCAGGCTAGCAGACAAAACAAACCCCCCGGTCGAGTACAGAACCAGGAGATA
CATAAGGTTATTTAGAGTCT

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MIELYYWPTPNGHKITLFLEEAGLEYEIKPINIGAGDQFQTDFLKISPNNRIPAIIDQAPADGGEPVSVFESGAILLYLA
EKTRKFLPNDIRQRNIVQEWLFWQVGGLGPMAGQNHHFSQYAPEKLPYAITRYVNETNRLYGVLNQHLQGKDFIAGDYSI
ADMACYPWIAPYKWQGQQLEDFPEINRWFQQIEQRPATVTAYEKGKQISQSAQLTAEKRKVLFGQTAKTQSSQV

Sequences:

>Translated_234_residues
MIELYYWPTPNGHKITLFLEEAGLEYEIKPINIGAGDQFQTDFLKISPNNRIPAIIDQAPADGGEPVSVFESGAILLYLA
EKTRKFLPNDIRQRNIVQEWLFWQVGGLGPMAGQNHHFSQYAPEKLPYAITRYVNETNRLYGVLNQHLQGKDFIAGDYSI
ADMACYPWIAPYKWQGQQLEDFPEINRWFQQIEQRPATVTAYEKGKQISQSAQLTAEKRKVLFGQTAKTQSSQV
>Mature_234_residues
MIELYYWPTPNGHKITLFLEEAGLEYEIKPINIGAGDQFQTDFLKISPNNRIPAIIDQAPADGGEPVSVFESGAILLYLA
EKTRKFLPNDIRQRNIVQEWLFWQVGGLGPMAGQNHHFSQYAPEKLPYAITRYVNETNRLYGVLNQHLQGKDFIAGDYSI
ADMACYPWIAPYKWQGQQLEDFPEINRWFQQIEQRPATVTAYEKGKQISQSAQLTAEKRKVLFGQTAKTQSSQV

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=207, Percent_Identity=57.487922705314, Blast_Score=253, Evalue=6e-69,
Organism=Escherichia coli, GI87082195, Length=224, Percent_Identity=39.2857142857143, Blast_Score=157, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6324100, Length=232, Percent_Identity=30.6034482758621, Blast_Score=113, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17933730, Length=227, Percent_Identity=27.7533039647577, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571670, Length=176, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17864592, Length=200, Percent_Identity=25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI85725204, Length=199, Percent_Identity=26.1306532663317, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17737923, Length=199, Percent_Identity=26.1306532663317, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24646249, Length=204, Percent_Identity=27.4509803921569, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17864594, Length=195, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17864598, Length=206, Percent_Identity=24.7572815533981, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45549270, Length=200, Percent_Identity=24, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646251, Length=200, Percent_Identity=26, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17864596, Length=190, Percent_Identity=23.1578947368421, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221378983, Length=230, Percent_Identity=26.5217391304348, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24646259, Length=230, Percent_Identity=26.9565217391304, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26758; Mature: 26758

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELYYWPTPNGHKITLFLEEAGLEYEIKPINIGAGDQFQTDFLKISPNNRIPAIIDQAP
CEEEEEECCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCEEEECCC
ADGGEPVSVFESGAILLYLAEKTRKFLPNDIRQRNIVQEWLFWQVGGLGPMAGQNHHFSQ
CCCCCCEEEECCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
YAPEKLPYAITRYVNETNRLYGVLNQHLQGKDFIAGDYSIADMACYPWIAPYKWQGQQLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCCCCCCCCCCCC
DFPEINRWFQQIEQRPATVTAYEKGKQISQSAQLTAEKRKVLFGQTAKTQSSQV
CCHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHEECCCCCCCCCCC
>Mature Secondary Structure
MIELYYWPTPNGHKITLFLEEAGLEYEIKPINIGAGDQFQTDFLKISPNNRIPAIIDQAP
CEEEEEECCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCEEEECCC
ADGGEPVSVFESGAILLYLAEKTRKFLPNDIRQRNIVQEWLFWQVGGLGPMAGQNHHFSQ
CCCCCCEEEECCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
YAPEKLPYAITRYVNETNRLYGVLNQHLQGKDFIAGDYSIADMACYPWIAPYKWQGQQLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCCCCCCCCCCCC
DFPEINRWFQQIEQRPATVTAYEKGKQISQSAQLTAEKRKVLFGQTAKTQSSQV
CCHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]