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The map label for this gene is yfhM [H]

Identifier: 158334359

GI number: 158334359

Start: 1146106

End: 1146978

Strand: Reverse

Name: yfhM [H]

Synonym: AM1_1180

Alternate gene names: 158334359

Gene position: 1146978-1146106 (Counterclockwise)

Preceding gene: 158334362

Following gene: 158334358

Centisome position: 17.64

GC content: 49.03

Gene sequence:

>873_bases
ATGGCTGACAATGAGCTAGGATCCGCTTTTCAACATGATTACATCACCACTAACCAGGTGCGGTTACATTATGTGACCCA
GGGGGAAGGGCCATTGATGCTGATGCTACATGGCTTCCCAGAATTTTGGTATTCTTGGCAACATCAAATTCCTGTTTTTG
CCCAGGACTATAAAGTTGTGGCCTTGGATATGCGAGGGTACAACGATAGTGAAAAGCCCCAGGATGTCAGTGCCTATCGC
ATGGCAGAGCTGATTAAGGATGTGCAGGGGGTGATTCAAGGGCTGGGCTATGACCAGTGCATCTTGGTCGCTCATGATTG
GGGTGGTGCGATCGCATGGCAATTCGCCTATGCTCACCCAGAGATGCTGCACAAACTGATCATTATGAACTTGCCCCATC
CCGCTAAGTTCCAAGAAGCGCTTATCAGTAATCCCCGGCAAATGTTGCGGAGCTGGTATATCGGCTTTTTCCAAGTGCCG
ATGTTGCCGGAGCTGATGTTTCAGGCTAATGACTACCGTGCGATCTCATCTATGTTTTCAGAACGGGCGACTAATAAAAA
TGCCTTTAGCCCTGCCGATTTAGAAGCATATAAAAACGCTGTGGCCAAGCGAGGTGCCCTCACTGCCATGATCAACTACT
ATCGCAGTAACCTTGATATGCTCTTGAAGCCCAAAGATTGGGGAGTCTTGGACGTTCCTACCCTGCTGCTATGGGGAGAA
GATGACTTTGCCCTGGGTAAGGAACTGACCTACGGCACCCAAGACTATGTTCGCGATCTTGAATTGCACTATCTCAGCGA
GTGTAGCCACTGGATCCAGCAAGAACAGCCCGAGCTGGTCAACCAATATATGCGAACCTTTTTGATGAGTTGA

Upstream 100 bases:

>100_bases
TCAGCCTGGAACTTTGAATTTATGGAAGGATGCCTCATAATCGCAATATTGGTCCTTCAAAAACACGATAACCCTAGATA
AGCTGAATCTTCTTAAAGCT

Downstream 100 bases:

>100_bases
GCCGAGTGGAATCCCACGAAAATATCACTATTGCTGAATATCAGGCTACCGCCAACAGTTTCCGGGAAGGCACCTGGGAC
CATGATGTTTCCCAGAACCG

Product: alpha/beta fold family hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MADNELGSAFQHDYITTNQVRLHYVTQGEGPLMLMLHGFPEFWYSWQHQIPVFAQDYKVVALDMRGYNDSEKPQDVSAYR
MAELIKDVQGVIQGLGYDQCILVAHDWGGAIAWQFAYAHPEMLHKLIIMNLPHPAKFQEALISNPRQMLRSWYIGFFQVP
MLPELMFQANDYRAISSMFSERATNKNAFSPADLEAYKNAVAKRGALTAMINYYRSNLDMLLKPKDWGVLDVPTLLLWGE
DDFALGKELTYGTQDYVRDLELHYLSECSHWIQQEQPELVNQYMRTFLMS

Sequences:

>Translated_290_residues
MADNELGSAFQHDYITTNQVRLHYVTQGEGPLMLMLHGFPEFWYSWQHQIPVFAQDYKVVALDMRGYNDSEKPQDVSAYR
MAELIKDVQGVIQGLGYDQCILVAHDWGGAIAWQFAYAHPEMLHKLIIMNLPHPAKFQEALISNPRQMLRSWYIGFFQVP
MLPELMFQANDYRAISSMFSERATNKNAFSPADLEAYKNAVAKRGALTAMINYYRSNLDMLLKPKDWGVLDVPTLLLWGE
DDFALGKELTYGTQDYVRDLELHYLSECSHWIQQEQPELVNQYMRTFLMS
>Mature_289_residues
ADNELGSAFQHDYITTNQVRLHYVTQGEGPLMLMLHGFPEFWYSWQHQIPVFAQDYKVVALDMRGYNDSEKPQDVSAYRM
AELIKDVQGVIQGLGYDQCILVAHDWGGAIAWQFAYAHPEMLHKLIIMNLPHPAKFQEALISNPRQMLRSWYIGFFQVPM
LPELMFQANDYRAISSMFSERATNKNAFSPADLEAYKNAVAKRGALTAMINYYRSNLDMLLKPKDWGVLDVPTLLLWGED
DFALGKELTYGTQDYVRDLELHYLSECSHWIQQEQPELVNQYMRTFLMS

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=297, Percent_Identity=43.4343434343434, Blast_Score=258, Evalue=3e-69,
Organism=Homo sapiens, GI218777840, Length=295, Percent_Identity=38.3050847457627, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI13376164, Length=295, Percent_Identity=38.3050847457627, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI27597073, Length=314, Percent_Identity=30.2547770700637, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17554012, Length=281, Percent_Identity=42.3487544483986, Blast_Score=243, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI17562298, Length=289, Percent_Identity=34.6020761245675, Blast_Score=216, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33625; Mature: 33494

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNELGSAFQHDYITTNQVRLHYVTQGEGPLMLMLHGFPEFWYSWQHQIPVFAQDYKVV
CCCCHHHHHHHHCCEEECCEEEEEEECCCCCEEEEEECCHHHHHHHCCCCCEEECCCEEE
ALDMRGYNDSEKPQDVSAYRMAELIKDVQGVIQGLGYDQCILVAHDWGGAIAWQFAYAHP
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEHHCCH
EMLHKLIIMNLPHPAKFQEALISNPRQMLRSWYIGFFQVPMLPELMFQANDYRAISSMFS
HHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHH
ERATNKNAFSPADLEAYKNAVAKRGALTAMINYYRSNLDMLLKPKDWGVLDVPTLLLWGE
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEECCEEEEECC
DDFALGKELTYGTQDYVRDLELHYLSECSHWIQQEQPELVNQYMRTFLMS
CCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADNELGSAFQHDYITTNQVRLHYVTQGEGPLMLMLHGFPEFWYSWQHQIPVFAQDYKVV
CCCHHHHHHHHCCEEECCEEEEEEECCCCCEEEEEECCHHHHHHHCCCCCEEECCCEEE
ALDMRGYNDSEKPQDVSAYRMAELIKDVQGVIQGLGYDQCILVAHDWGGAIAWQFAYAHP
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEHHCCH
EMLHKLIIMNLPHPAKFQEALISNPRQMLRSWYIGFFQVPMLPELMFQANDYRAISSMFS
HHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHH
ERATNKNAFSPADLEAYKNAVAKRGALTAMINYYRSNLDMLLKPKDWGVLDVPTLLLWGE
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEECCEEEEECC
DDFALGKELTYGTQDYVRDLELHYLSECSHWIQQEQPELVNQYMRTFLMS
CCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]