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The map label for this gene is putP [H]

Identifier: 158334345

GI number: 158334345

Start: 1136177

End: 1137667

Strand: Direct

Name: putP [H]

Synonym: AM1_1166

Alternate gene names: 158334345

Gene position: 1136177-1137667 (Clockwise)

Preceding gene: 158334344

Following gene: 158334346

Centisome position: 17.47

GC content: 48.02

Gene sequence:

>1491_bases
ATGGCTGACTCATTGCTTCCTGGGGGTATCATTGCATTCCTAATCGCCACCCTGGGCATCGGCGTTTATGCGTCCAAACA
AATTAAAGGAGACAGTGTTAACTTTTTAGTCGCAGGTCGAGGGCTAACGCTGCCCTTAGCAGCCGCCACACTAATGGCCC
AATCTGTCGACTCAAATGCCACCCTGGGCAATACGGACCTGGCTTCAGAATTCGGATTCTGGGCAGGAGCTTCACTACCC
CTGGGCTTAGCCCTTTGTCTCTTCTTGACCGCTCTGTTTTTCGCCAAACCAATGAATCGGATGGGGTTAATCACATTGCC
CGATTTCTATCGGGTGAAATATGGGCGGACCACCGAATGGGTAGCAGCCGTACTGATGGTCATTAGCTTCTCTTTCTTAC
TCGCAGGTAATTTAGTTGCAGGAGGATATCTTTTCCAAACCTTTTTAGGGATGTCCTACGCCATTGGGGTGTTGTTGCTT
GCGGTCATCGTTTTGATCTACACCGCTAGTGGCGGCTTATTTGCCGTTGCCTATACTGATGCGATTCAAGTGCTGATTGC
TTTAGTGGGATCACTGTGTCTCTTCGGTTATATCGGACTGAATTTCAGCTTCAATATTCCAGTGGGTACCGGTCCTTTAG
CCTTAGCACAGATGACTAGCCCTGCATCAGGTTCCCTCGTCAATTGGGCGACGCTGTTAGCCCTGGGACTGGGAGATATT
GTTGCCATTGACTTTATGGCTCGAATCTTTTCTGCAGAAAGTCCTGAAACGGCTCAAAGAGCTTGCCTGATTGGCTCTCT
AGGTACCGTAGTGATTGGTGTTCCGTTCTCGATCATCGCTCTCTCTACACCTGAAATTTTGGCATCAGCTGGTGTTACGG
CTGAAGGTCCTATCCTTTATGCGTTGTTGCAGAATGTTGTGCCTTCCTCTTTGGCTCTTTTAGTCATTGTGGCGATTTTA
TCGGCCTCTCTTTCAACTGCAGATGGCGCTATTTTGGGAACTTCTTCGGTGATCGCTCACAACATCCTGGGCATTCGCCA
TAATCAGCCCAGTGCGAGTGGTGATCGACTTCTCGCTTTGACCCGCTCGATGGCCGTCGTCATCACTGCCATGGGAGTGT
TTTTTGCTTTAAGAGTTCCCCAAACCGGGGTTTTACTCCTATTGGCGTTTGATCTGGGGTTTGCCGGATTGTTGGTTCCC
TTGGCCGGGGGACTATTCTATTCAAAAGCAACGAAAGAAGGTGCATTAGCTTGCATTATTGTGGGTTCGTTGACTCGGTT
GCTTTTATTCGCACTGATGCCTGTGACATTTGGCATTGATAATACCCTTCTTTACATACCTAATAGTATTTTCACCGCTG
ATTTTGATGGCATTCCCACCCTAATCAGCCCTTTAATTGGATTATGCCTATTTTTAGTGATTTCTAAGTTAACCTATCGG
CCCTTTCCTATTGTGGAACAGCCCGTTCAGCGCACCCTCCAGAAGATTTAA

Upstream 100 bases:

>100_bases
CAAATATTAGCCAGTTTGTTGAGATGGATACAATGTGACGCTTTTCCTATCTTTATGATGTCACCAATGGGCAATTTCAC
CTGACGTAGGGATTTCAACC

Downstream 100 bases:

>100_bases
GCCTTGTCTCCTAGTTTAAAACCGCTGGAGTGATGATTACGTTTGGTTTTGGCACAAGAAATCACTAAGAGCTAGCACGA
CTTACGATGGGAGTTTTAGA

Product: Na+/proline symporter PutP

Products: Na (I) [Cytoplasm]; pantothenate [Cytoplasm] [C]

Alternate protein names: Proline permease 2 [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MADSLLPGGIIAFLIATLGIGVYASKQIKGDSVNFLVAGRGLTLPLAAATLMAQSVDSNATLGNTDLASEFGFWAGASLP
LGLALCLFLTALFFAKPMNRMGLITLPDFYRVKYGRTTEWVAAVLMVISFSFLLAGNLVAGGYLFQTFLGMSYAIGVLLL
AVIVLIYTASGGLFAVAYTDAIQVLIALVGSLCLFGYIGLNFSFNIPVGTGPLALAQMTSPASGSLVNWATLLALGLGDI
VAIDFMARIFSAESPETAQRACLIGSLGTVVIGVPFSIIALSTPEILASAGVTAEGPILYALLQNVVPSSLALLVIVAIL
SASLSTADGAILGTSSVIAHNILGIRHNQPSASGDRLLALTRSMAVVITAMGVFFALRVPQTGVLLLLAFDLGFAGLLVP
LAGGLFYSKATKEGALACIIVGSLTRLLLFALMPVTFGIDNTLLYIPNSIFTADFDGIPTLISPLIGLCLFLVISKLTYR
PFPIVEQPVQRTLQKI

Sequences:

>Translated_496_residues
MADSLLPGGIIAFLIATLGIGVYASKQIKGDSVNFLVAGRGLTLPLAAATLMAQSVDSNATLGNTDLASEFGFWAGASLP
LGLALCLFLTALFFAKPMNRMGLITLPDFYRVKYGRTTEWVAAVLMVISFSFLLAGNLVAGGYLFQTFLGMSYAIGVLLL
AVIVLIYTASGGLFAVAYTDAIQVLIALVGSLCLFGYIGLNFSFNIPVGTGPLALAQMTSPASGSLVNWATLLALGLGDI
VAIDFMARIFSAESPETAQRACLIGSLGTVVIGVPFSIIALSTPEILASAGVTAEGPILYALLQNVVPSSLALLVIVAIL
SASLSTADGAILGTSSVIAHNILGIRHNQPSASGDRLLALTRSMAVVITAMGVFFALRVPQTGVLLLLAFDLGFAGLLVP
LAGGLFYSKATKEGALACIIVGSLTRLLLFALMPVTFGIDNTLLYIPNSIFTADFDGIPTLISPLIGLCLFLVISKLTYR
PFPIVEQPVQRTLQKI
>Mature_495_residues
ADSLLPGGIIAFLIATLGIGVYASKQIKGDSVNFLVAGRGLTLPLAAATLMAQSVDSNATLGNTDLASEFGFWAGASLPL
GLALCLFLTALFFAKPMNRMGLITLPDFYRVKYGRTTEWVAAVLMVISFSFLLAGNLVAGGYLFQTFLGMSYAIGVLLLA
VIVLIYTASGGLFAVAYTDAIQVLIALVGSLCLFGYIGLNFSFNIPVGTGPLALAQMTSPASGSLVNWATLLALGLGDIV
AIDFMARIFSAESPETAQRACLIGSLGTVVIGVPFSIIALSTPEILASAGVTAEGPILYALLQNVVPSSLALLVIVAILS
ASLSTADGAILGTSSVIAHNILGIRHNQPSASGDRLLALTRSMAVVITAMGVFFALRVPQTGVLLLLAFDLGFAGLLVPL
AGGLFYSKATKEGALACIIVGSLTRLLLFALMPVTFGIDNTLLYIPNSIFTADFDGIPTLISPLIGLCLFLVISKLTYRP
FPIVEQPVQRTLQKI

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:
GO:0005215: Na+/proline symporter PutP, putative
GO:0005886: Na+/proline symporter PutP, putative
GO:0006810: Na+/proline symporter PutP, putative
GO:0006811: Na+/proline symporter PutP, putative
GO:0006814: Na+/proline symporter PutP, putative
GO:0016020: Na+/proline symporter PutP, putative
GO:0016021: Na+/proline symporter PutP, putative
GO:0055085: Na+/proline symporter PutP, putative

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI11141885, Length=445, Percent_Identity=27.4157303370787, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI14140236, Length=199, Percent_Identity=25.1256281407035, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI87082237, Length=438, Percent_Identity=24.8858447488584, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787251, Length=345, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539284, Length=457, Percent_Identity=26.0393873085339, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648033, Length=442, Percent_Identity=26.4705882352941, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356865, Length=442, Percent_Identity=26.4705882352941, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 51695; Mature: 51564

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSLLPGGIIAFLIATLGIGVYASKQIKGDSVNFLVAGRGLTLPLAAATLMAQSVDSNA
CCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCC
TLGNTDLASEFGFWAGASLPLGLALCLFLTALFFAKPMNRMGLITLPDFYRVKYGRTTEW
CCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHEECCCCHHH
VAAVLMVISFSFLLAGNLVAGGYLFQTFLGMSYAIGVLLLAVIVLIYTASGGLFAVAYTD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHH
AIQVLIALVGSLCLFGYIGLNFSFNIPVGTGPLALAQMTSPASGSLVNWATLLALGLGDI
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VAIDFMARIFSAESPETAQRACLIGSLGTVVIGVPFSIIALSTPEILASAGVTAEGPILY
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCHHEEEECCHHHHHHCCCCCCCHHHH
ALLQNVVPSSLALLVIVAILSASLSTADGAILGTSSVIAHNILGIRHNQPSASGDRLLAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHEEECCCCCCCCCHHHHH
TRSMAVVITAMGVFFALRVPQTGVLLLLAFDLGFAGLLVPLAGGLFYSKATKEGALACII
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEH
VGSLTRLLLFALMPVTFGIDNTLLYIPNSIFTADFDGIPTLISPLIGLCLFLVISKLTYR
HHHHHHHHHHHHHHHHCCCCCEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
PFPIVEQPVQRTLQKI
CCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ADSLLPGGIIAFLIATLGIGVYASKQIKGDSVNFLVAGRGLTLPLAAATLMAQSVDSNA
CCCCCCHHHHHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCC
TLGNTDLASEFGFWAGASLPLGLALCLFLTALFFAKPMNRMGLITLPDFYRVKYGRTTEW
CCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHEECCCCHHH
VAAVLMVISFSFLLAGNLVAGGYLFQTFLGMSYAIGVLLLAVIVLIYTASGGLFAVAYTD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHH
AIQVLIALVGSLCLFGYIGLNFSFNIPVGTGPLALAQMTSPASGSLVNWATLLALGLGDI
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VAIDFMARIFSAESPETAQRACLIGSLGTVVIGVPFSIIALSTPEILASAGVTAEGPILY
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCHHEEEECCHHHHHHCCCCCCCHHHH
ALLQNVVPSSLALLVIVAILSASLSTADGAILGTSSVIAHNILGIRHNQPSASGDRLLAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHEEECCCCCCCCCHHHHH
TRSMAVVITAMGVFFALRVPQTGVLLLLAFDLGFAGLLVPLAGGLFYSKATKEGALACII
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEH
VGSLTRLLLFALMPVTFGIDNTLLYIPNSIFTADFDGIPTLISPLIGLCLFLVISKLTYR
HHHHHHHHHHHHHHHHCCCCCEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
PFPIVEQPVQRTLQKI
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; pantothenate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + pantothenate [Periplasm] = Na (I) [Cytoplasm] + pantothenate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA