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The map label for this gene is azoB [H]

Identifier: 158334293

GI number: 158334293

Start: 1090572

End: 1091552

Strand: Direct

Name: azoB [H]

Synonym: AM1_1112

Alternate gene names: 158334293

Gene position: 1090572-1091552 (Clockwise)

Preceding gene: 158334292

Following gene: 158334294

Centisome position: 16.77

GC content: 48.62

Gene sequence:

>981_bases
ATGAATCTATTAATTGTCGGTGCTACTGGAACACTTGGCAGGCAAATTGCCCGTCGGGCCTTAGATGAGGGACATGAGGT
CACTTGTTTAGTGCGCGCACCTCGCGCCGCCACGTTTCTAAGGGAATGGGGTGCTTCCTTGATTAAAGGGGATTTACGAG
ATCCAGAAACCCTCAAATTGGCCATGGAAGGCAACACCGCCGTTATTGACGCTGCAACGGTTCGAGCCACAGATTCCATC
GGCATTCGAGAAGTGGATTGGGATGGTAAGGTAGCCCTGATTCAGGCCGCTAAAGCTGCTGGCATCCAGCGATTTGTCTT
TTTCTCCATTTTGGGCGCAGAGAATTATCCCAAGGTGCCCTTGATGGACATTAAGAACTGCACCGAGCTGTTTATCAAAG
AATCTGGCCTAAACTACACGATTCTGCGCCCCTGCGGTTTCTTCCAGGGACTGATTGGACAGTATGCCATTCCCATCCTT
GAGGACCAGTCCGTTTGGGTGATGAATGAAGCCACTTCAACGGCATACATGGATACGCAAGATATCGCCAAGTTTGCGGT
GAATGCCCTCTCCCATCCTGAAACTGAGAACAAAACCTTTGATTTAGCGGGCCCCAAAGATTGGTCCCCTGAACAAATTG
TGGCCCTTTGCGAGAATATTGCCAATCAACCTGCCAAAGTGACACGGATGCCGATTGGCTTATTACGGAGTGGGCAAAAA
ATTGCTCGTTTCTTCCAATGGAGCTGGAACATTGCTGATCGCTTAGCGTTTAGTGAAGTGATTACGTCTCAGGCGCCGAT
CACCGTCCCGATGACAGAGACCTGTAAGGTATTTGGAGTAGATGAATCTGAGATTTCTACTCTGGAAGCCTATATGCAAG
AATATTTTGATCGGATTCTCAAGAAACTGAAGCAGCTCGAATACGAGAAGAACCAGAAACAATCTCGCAAACGGAGCCCC
TTCAAAACTCCCAGTTCCTAA

Upstream 100 bases:

>100_bases
AATCCCCTTAGTGAGTAGAGAAATCTCTTGACTTCACCCATACAACCCTCAACTTTCTGATAAAATATTACAAAAGTTAA
GATTTGTAATTTAAGACCTC

Downstream 100 bases:

>100_bases
CGATAGGTTGAACCTTGAGCGTGCCGAAAGCTGGCATTATCTATAACGAACTCAAGCCGTTCGCTTGCCGAATTGCGACT
GAACTCAAAGACAAGCTCCA

Product: NAD dependent epimerase/dehydratase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNLLIVGATGTLGRQIARRALDEGHEVTCLVRAPRAATFLREWGASLIKGDLRDPETLKLAMEGNTAVIDAATVRATDSI
GIREVDWDGKVALIQAAKAAGIQRFVFFSILGAENYPKVPLMDIKNCTELFIKESGLNYTILRPCGFFQGLIGQYAIPIL
EDQSVWVMNEATSTAYMDTQDIAKFAVNALSHPETENKTFDLAGPKDWSPEQIVALCENIANQPAKVTRMPIGLLRSGQK
IARFFQWSWNIADRLAFSEVITSQAPITVPMTETCKVFGVDESEISTLEAYMQEYFDRILKKLKQLEYEKNQKQSRKRSP
FKTPSS

Sequences:

>Translated_326_residues
MNLLIVGATGTLGRQIARRALDEGHEVTCLVRAPRAATFLREWGASLIKGDLRDPETLKLAMEGNTAVIDAATVRATDSI
GIREVDWDGKVALIQAAKAAGIQRFVFFSILGAENYPKVPLMDIKNCTELFIKESGLNYTILRPCGFFQGLIGQYAIPIL
EDQSVWVMNEATSTAYMDTQDIAKFAVNALSHPETENKTFDLAGPKDWSPEQIVALCENIANQPAKVTRMPIGLLRSGQK
IARFFQWSWNIADRLAFSEVITSQAPITVPMTETCKVFGVDESEISTLEAYMQEYFDRILKKLKQLEYEKNQKQSRKRSP
FKTPSS
>Mature_326_residues
MNLLIVGATGTLGRQIARRALDEGHEVTCLVRAPRAATFLREWGASLIKGDLRDPETLKLAMEGNTAVIDAATVRATDSI
GIREVDWDGKVALIQAAKAAGIQRFVFFSILGAENYPKVPLMDIKNCTELFIKESGLNYTILRPCGFFQGLIGQYAIPIL
EDQSVWVMNEATSTAYMDTQDIAKFAVNALSHPETENKTFDLAGPKDWSPEQIVALCENIANQPAKVTRMPIGLLRSGQK
IARFFQWSWNIADRLAFSEVITSQAPITVPMTETCKVFGVDESEISTLEAYMQEYFDRILKKLKQLEYEKNQKQSRKRSP
FKTPSS

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 36390; Mature: 36390

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLIVGATGTLGRQIARRALDEGHEVTCLVRAPRAATFLREWGASLIKGDLRDPETLKL
CCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEE
AMEGNTAVIDAATVRATDSIGIREVDWDGKVALIQAAKAAGIQRFVFFSILGAENYPKVP
EECCCEEEEEEEEEEECCCCCCEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCC
LMDIKNCTELFIKESGLNYTILRPCGFFQGLIGQYAIPILEDQSVWVMNEATSTAYMDTQ
CHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCHH
DIAKFAVNALSHPETENKTFDLAGPKDWSPEQIVALCENIANQPAKVTRMPIGLLRSGQK
HHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHCCHHHHHCCHH
IARFFQWSWNIADRLAFSEVITSQAPITVPMTETCKVFGVDESEISTLEAYMQEYFDRIL
HHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
KKLKQLEYEKNQKQSRKRSPFKTPSS
HHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNLLIVGATGTLGRQIARRALDEGHEVTCLVRAPRAATFLREWGASLIKGDLRDPETLKL
CCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEE
AMEGNTAVIDAATVRATDSIGIREVDWDGKVALIQAAKAAGIQRFVFFSILGAENYPKVP
EECCCEEEEEEEEEEECCCCCCEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCC
LMDIKNCTELFIKESGLNYTILRPCGFFQGLIGQYAIPILEDQSVWVMNEATSTAYMDTQ
CHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCHH
DIAKFAVNALSHPETENKTFDLAGPKDWSPEQIVALCENIANQPAKVTRMPIGLLRSGQK
HHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHCCHHHHHCCHH
IARFFQWSWNIADRLAFSEVITSQAPITVPMTETCKVFGVDESEISTLEAYMQEYFDRIL
HHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
KKLKQLEYEKNQKQSRKRSPFKTPSS
HHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]