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The map label for this gene is 158334109

Identifier: 158334109

GI number: 158334109

Start: 893728

End: 895185

Strand: Direct

Name: 158334109

Synonym: AM1_0925

Alternate gene names: NA

Gene position: 893728-895185 (Clockwise)

Preceding gene: 158334108

Following gene: 158334112

Centisome position: 13.74

GC content: 50.34

Gene sequence:

>1458_bases
ATGCAAACTCAAGACCGCGATACCCAACGTGACCAATTCGCGCAAGGAAAGCCACTGTGGGCCCGTGCGATCGCAACCCC
CGCTACGGAATTTGGCCCCACCGCCCTGCCCCTGTTGTCCGGCCAAATCCCTGAAGGTCTCCAGGGCACCCTATATCGCA
ATGGCCCTGGGCGTTTATCTCGCCAGGGACAAACTGTAGATCACTGGTTTGACGGAGACGGCGGTATTTTAGCCGTTCAC
GTGGACGATGGAGGAGCAACAGCCTGTTACCGCTATGTCCAAACCCAAGAACTTCAGGCAGAAGAAGCCAAAGATCAATT
TCTCTACTCTGGCTATGGACAAATGGCCCCTGGTCCAGTTTGGAAACGGTGGGGGGTTCCGCCAAAAAATGTAGCTAATA
CCTCAGTTTTGGCCTTGCCTGACAAATTGCTAGCCCTTTGGGAAGGGGGCAACCCACATGCCCTTGATCCCGTTACCCTA
GAAACCCAGGGCTTGGATCCCCTTTCAGGTTTAACTCCAAAGCTCACCTATTCGGCCCATCCCAAACGCGATCCACACAC
AGGCAACATTTATAACTTTGGCGTTATCTTTGGCCCCAAAACCTATATTCAGATCTATTGCAGCCATGCCTCAGGCGACA
TTTATCGGCAAGCTTCAATTCCCTTACCGGGATTATCTATGGTTCATGATTTTGTCCTAGCGGGGCGCTATCTCATCTTC
ATCGTGCCACCCCTGAGATTACAAATGCTCCCCATTCTGTTGGGCACCAAAAGTTTAAGCGATGCCTTGCAGTGGCAACC
CCATCAAGGAACGCAAATCATCGTCATTGATCGGGACACCTTTAAGGAGGTCACTCGCATAGATACCGATCCTTGGTTTC
AATGGCACTTTAGCAATGGCCATGAAGATGCTGCCGGGAATATTGTTGTGGACTATGTTCGCTATGACGATTTCCAAACC
AATCAGTGGTTGAAAGAAGTGGTCACTGGCTATCCGCAAGCCCCTGCGACTGGGAAGTTGTGGCAACTACAGATTAATCC
TCAATCCGGCCAAGTCCTGCACAATGAACCGTTGTTTAACTTTGGTTGTGAGTTTCCTGTCATTTCGTCCCTGGAAACCG
GACAAAAGACCACCGCCATTTACCTGAACCATCATTCCCATACTCAACGACCGGTTGAGGAAATGTTTGACTGCATCGCT
CGCATTGATGGCAAAACCTCAGCTGTCACGAGTATGGCCTTTCCTTCTGGCTGTTACCCCATGGAAGCCATTTATGCTCC
TGATCCCAACACTTTTGATCAAGGGTGGTTGTTAACCGTGGTCTTTGATGGCAACCAGGATCAAAGTACCGTGCAAATTT
TTTCAGCGAATCAGCTAGATGCAGGTCCTATTGCCGTTTTAGGACTGCCCGAGATTATCCCCTTTGGGTTTCATGGCGCT
TGGCAAGCTAGGGCGTAA

Upstream 100 bases:

>100_bases
TAACCGCTCTGTTTTTTAACAAGACAGCCCTCTAGTTCAGAGGGCTGTCTTGTATGGTGCGCTAAAGTTTATCCATTGTT
TCAGTCATATTTTGGTGTCC

Downstream 100 bases:

>100_bases
GCGCCTCTGTGCTCAACTCTACTCAGCCCCCCGCTTTAATGCCCGCTCCATATCTCGTTTATCCTGACGTTTCTTAATCG
ACTCCCGCTTATCGTGGAGT

Product: hypothetical protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MQTQDRDTQRDQFAQGKPLWARAIATPATEFGPTALPLLSGQIPEGLQGTLYRNGPGRLSRQGQTVDHWFDGDGGILAVH
VDDGGATACYRYVQTQELQAEEAKDQFLYSGYGQMAPGPVWKRWGVPPKNVANTSVLALPDKLLALWEGGNPHALDPVTL
ETQGLDPLSGLTPKLTYSAHPKRDPHTGNIYNFGVIFGPKTYIQIYCSHASGDIYRQASIPLPGLSMVHDFVLAGRYLIF
IVPPLRLQMLPILLGTKSLSDALQWQPHQGTQIIVIDRDTFKEVTRIDTDPWFQWHFSNGHEDAAGNIVVDYVRYDDFQT
NQWLKEVVTGYPQAPATGKLWQLQINPQSGQVLHNEPLFNFGCEFPVISSLETGQKTTAIYLNHHSHTQRPVEEMFDCIA
RIDGKTSAVTSMAFPSGCYPMEAIYAPDPNTFDQGWLLTVVFDGNQDQSTVQIFSANQLDAGPIAVLGLPEIIPFGFHGA
WQARA

Sequences:

>Translated_485_residues
MQTQDRDTQRDQFAQGKPLWARAIATPATEFGPTALPLLSGQIPEGLQGTLYRNGPGRLSRQGQTVDHWFDGDGGILAVH
VDDGGATACYRYVQTQELQAEEAKDQFLYSGYGQMAPGPVWKRWGVPPKNVANTSVLALPDKLLALWEGGNPHALDPVTL
ETQGLDPLSGLTPKLTYSAHPKRDPHTGNIYNFGVIFGPKTYIQIYCSHASGDIYRQASIPLPGLSMVHDFVLAGRYLIF
IVPPLRLQMLPILLGTKSLSDALQWQPHQGTQIIVIDRDTFKEVTRIDTDPWFQWHFSNGHEDAAGNIVVDYVRYDDFQT
NQWLKEVVTGYPQAPATGKLWQLQINPQSGQVLHNEPLFNFGCEFPVISSLETGQKTTAIYLNHHSHTQRPVEEMFDCIA
RIDGKTSAVTSMAFPSGCYPMEAIYAPDPNTFDQGWLLTVVFDGNQDQSTVQIFSANQLDAGPIAVLGLPEIIPFGFHGA
WQARA
>Mature_485_residues
MQTQDRDTQRDQFAQGKPLWARAIATPATEFGPTALPLLSGQIPEGLQGTLYRNGPGRLSRQGQTVDHWFDGDGGILAVH
VDDGGATACYRYVQTQELQAEEAKDQFLYSGYGQMAPGPVWKRWGVPPKNVANTSVLALPDKLLALWEGGNPHALDPVTL
ETQGLDPLSGLTPKLTYSAHPKRDPHTGNIYNFGVIFGPKTYIQIYCSHASGDIYRQASIPLPGLSMVHDFVLAGRYLIF
IVPPLRLQMLPILLGTKSLSDALQWQPHQGTQIIVIDRDTFKEVTRIDTDPWFQWHFSNGHEDAAGNIVVDYVRYDDFQT
NQWLKEVVTGYPQAPATGKLWQLQINPQSGQVLHNEPLFNFGCEFPVISSLETGQKTTAIYLNHHSHTQRPVEEMFDCIA
RIDGKTSAVTSMAFPSGCYPMEAIYAPDPNTFDQGWLLTVVFDGNQDQSTVQIFSANQLDAGPIAVLGLPEIIPFGFHGA
WQARA

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI82617628, Length=524, Percent_Identity=24.4274809160305, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI82617624, Length=524, Percent_Identity=24.4274809160305, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI74027270, Length=320, Percent_Identity=25.625, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI32564756, Length=307, Percent_Identity=24.7557003257329, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71998242, Length=307, Percent_Identity=25.4071661237785, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 53712; Mature: 53712

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQDRDTQRDQFAQGKPLWARAIATPATEFGPTALPLLSGQIPEGLQGTLYRNGPGRLS
CCCCCCCCHHHHHHCCCCHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCEEEECCCCCCC
RQGQTVDHWFDGDGGILAVHVDDGGATACYRYVQTQELQAEEAKDQFLYSGYGQMAPGPV
CCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
WKRWGVPPKNVANTSVLALPDKLLALWEGGNPHALDPVTLETQGLDPLSGLTPKLTYSAH
HHHCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCEEECCC
PKRDPHTGNIYNFGVIFGPKTYIQIYCSHASGDIYRQASIPLPGLSMVHDFVLAGRYLIF
CCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHCCEEEE
IVPPLRLQMLPILLGTKSLSDALQWQPHQGTQIIVIDRDTFKEVTRIDTDPWFQWHFSNG
EECCHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEECHHHHHHHHCCCCCCEEEEECCC
HEDAAGNIVVDYVRYDDFQTNQWLKEVVTGYPQAPATGKLWQLQINPQSGQVLHNEPLFN
CCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEECCCCHH
FGCEFPVISSLETGQKTTAIYLNHHSHTQRPVEEMFDCIARIDGKTSAVTSMAFPSGCYP
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHEEEECCCCCCCC
MEAIYAPDPNTFDQGWLLTVVFDGNQDQSTVQIFSANQLDAGPIAVLGLPEIIPFGFHGA
CEEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEECHHHCCCCCCCC
WQARA
CCCCC
>Mature Secondary Structure
MQTQDRDTQRDQFAQGKPLWARAIATPATEFGPTALPLLSGQIPEGLQGTLYRNGPGRLS
CCCCCCCCHHHHHHCCCCHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCEEEECCCCCCC
RQGQTVDHWFDGDGGILAVHVDDGGATACYRYVQTQELQAEEAKDQFLYSGYGQMAPGPV
CCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
WKRWGVPPKNVANTSVLALPDKLLALWEGGNPHALDPVTLETQGLDPLSGLTPKLTYSAH
HHHCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCEEECCC
PKRDPHTGNIYNFGVIFGPKTYIQIYCSHASGDIYRQASIPLPGLSMVHDFVLAGRYLIF
CCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHCCEEEE
IVPPLRLQMLPILLGTKSLSDALQWQPHQGTQIIVIDRDTFKEVTRIDTDPWFQWHFSNG
EECCHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEECHHHHHHHHCCCCCCEEEEECCC
HEDAAGNIVVDYVRYDDFQTNQWLKEVVTGYPQAPATGKLWQLQINPQSGQVLHNEPLFN
CCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEECCCCHH
FGCEFPVISSLETGQKTTAIYLNHHSHTQRPVEEMFDCIARIDGKTSAVTSMAFPSGCYP
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHEEEECCCCCCCC
MEAIYAPDPNTFDQGWLLTVVFDGNQDQSTVQIFSANQLDAGPIAVLGLPEIIPFGFHGA
CEEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEECHHHCCCCCCCC
WQARA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]