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The map label for this gene is amtB [C]

Identifier: 158334101

GI number: 158334101

Start: 885863

End: 887341

Strand: Direct

Name: amtB [C]

Synonym: AM1_0917

Alternate gene names: 158334101

Gene position: 885863-887341 (Clockwise)

Preceding gene: 158334098

Following gene: 158334108

Centisome position: 13.62

GC content: 49.02

Gene sequence:

>1479_bases
ATGTCTAGAAATTTAAAGCCTAAGCCACCCGGCCAGCCGCGCCATCGACGCGCCTCTCGGCGTTGGTCAATGTTGAAAAA
ACCGATTTATTTATATTTAACTGCGGCCATTCTATTGGTATGGAGCAGTGCAGCGGTTGCTCAAGAAGCCCCTGCATCTG
TTTTTTCTGGCGAAGCTAAAGTCGTTGCAGACACCATTTGGGTGCTCTTTGCAGCTTCCCTCGTATTCTTTATGAATGCT
GGCTTTGCCATGCTGGAAACGGGGCTTTGTCGGAGTAAAAATGCGGTCAACCTGCTGGCAAAAAACCTGATTGTGTTTGG
TTTGGTGAGTTTGGCTTACTGGGCCGTAGGGTTTGGCATCATGTTTGGCGACGGCAATGAGTTTATGGGGACGACTGGCT
TTTTCTTGGGAGGCAACGACAACAGTCCCGCCATTGGTGAAGCCTATGATGGTGTATTTTCTGCCCTCAATTGGGCTGGG
ATTCCCCTCAACGCCAAATTTTTCTACCAATTGATGTTTGCCGGTACTGCAGCCACCATCGTGTCTGGTGCGATTGCTGA
GCGAGCCAGATTTGTTGGATTCTTTATTTTTAGTATTTTGTTAACCTCCATTTGCTATCCCATGGTGGGCCATTGGGTTT
GGGGCGGTGGCTTCCTGCAAAAGATGGGGTTTTATGACTTTGCAGGCTCTACCGTGGTCCATATGGTCGGAGGATGGGCT
GCCCTAGTGGGTGCTGCATTGATTGGCCCCCGAACAGCTCGCTATTTTGAAGGCAAAAGCTATCCCATTCCGGGCCATAA
CCTCAGTATCGCGACACTGGGTGGATTGATTTTATGGTTAGGTTGGTTTGGATTTAATGGTGGCAGCACGATGGCTGCTG
ACCCAGCTGCCATTAGCCATATTATTGTGATCACCAACCTTGCTGGTGCTGCGGGTGGTGTGGCAGGTACTATCACGGCC
TGGATCTATTTCGGTAAGCCTGACTTAACCATGACCATCAACGGTATGCTAATTGGCTTGGTGAGTATCACTGCAGGCTG
CGCTTTTGTGACATCCGGTAGTGCCGTTTTGATCGGCTTTGTTGGCGGTATAGTTGTCGTCTTTGCCGTTGACGTCTTTG
ATAACTTCAAAATTGACGACCCTGTGGGTGCTTTATCTGTCCATATGGTGGGAGGCTTCTGGGGGACTCTAGCGGTTGGC
CTGTTTAGTGTCGGTCCAGAGAAGTTTAGTTGGTACACTGCCGATTCTGGTCCTGCTATGGGTCTGTTATCGGGTGGTTC
TTTTAGCCAAGTCGGTATTCAACTTCTAGGTCTAGCGATTGTCAGTGGTTTTACAGTGGCTTTTAGCTATATTGTGTGGA
CTGCCCTCAAATATCTCTCAGGTATTCGAGTCACCCTGGAAGACGAGAAACTCGGTTTGGATCTTAGCGAACATGCCATG
GAAGCCTATCCTGGTTTCTTAGGAGATGAGCATCTTTAA

Upstream 100 bases:

>100_bases
TCTGGACAAAGATTTGATAGTTGTGTGTGTCTGTCCCAATGATGATTCTCTTGCTAGATAAACAACTGTGTGAATGAGGT
CAAGGAGCTGTAAAGCACTC

Downstream 100 bases:

>100_bases
AAGATAGAGCTAAAAAATAGGGCTAGATTTTAGAATCTAATCACTATTCAATATAGAAAAGCGGCTGTCGATGACAGCCG
CTTTTTAATTCCGAAAATCA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSRNLKPKPPGQPRHRRASRRWSMLKKPIYLYLTAAILLVWSSAAVAQEAPASVFSGEAKVVADTIWVLFAASLVFFMNA
GFAMLETGLCRSKNAVNLLAKNLIVFGLVSLAYWAVGFGIMFGDGNEFMGTTGFFLGGNDNSPAIGEAYDGVFSALNWAG
IPLNAKFFYQLMFAGTAATIVSGAIAERARFVGFFIFSILLTSICYPMVGHWVWGGGFLQKMGFYDFAGSTVVHMVGGWA
ALVGAALIGPRTARYFEGKSYPIPGHNLSIATLGGLILWLGWFGFNGGSTMAADPAAISHIIVITNLAGAAGGVAGTITA
WIYFGKPDLTMTINGMLIGLVSITAGCAFVTSGSAVLIGFVGGIVVVFAVDVFDNFKIDDPVGALSVHMVGGFWGTLAVG
LFSVGPEKFSWYTADSGPAMGLLSGGSFSQVGIQLLGLAIVSGFTVAFSYIVWTALKYLSGIRVTLEDEKLGLDLSEHAM
EAYPGFLGDEHL

Sequences:

>Translated_492_residues
MSRNLKPKPPGQPRHRRASRRWSMLKKPIYLYLTAAILLVWSSAAVAQEAPASVFSGEAKVVADTIWVLFAASLVFFMNA
GFAMLETGLCRSKNAVNLLAKNLIVFGLVSLAYWAVGFGIMFGDGNEFMGTTGFFLGGNDNSPAIGEAYDGVFSALNWAG
IPLNAKFFYQLMFAGTAATIVSGAIAERARFVGFFIFSILLTSICYPMVGHWVWGGGFLQKMGFYDFAGSTVVHMVGGWA
ALVGAALIGPRTARYFEGKSYPIPGHNLSIATLGGLILWLGWFGFNGGSTMAADPAAISHIIVITNLAGAAGGVAGTITA
WIYFGKPDLTMTINGMLIGLVSITAGCAFVTSGSAVLIGFVGGIVVVFAVDVFDNFKIDDPVGALSVHMVGGFWGTLAVG
LFSVGPEKFSWYTADSGPAMGLLSGGSFSQVGIQLLGLAIVSGFTVAFSYIVWTALKYLSGIRVTLEDEKLGLDLSEHAM
EAYPGFLGDEHL
>Mature_491_residues
SRNLKPKPPGQPRHRRASRRWSMLKKPIYLYLTAAILLVWSSAAVAQEAPASVFSGEAKVVADTIWVLFAASLVFFMNAG
FAMLETGLCRSKNAVNLLAKNLIVFGLVSLAYWAVGFGIMFGDGNEFMGTTGFFLGGNDNSPAIGEAYDGVFSALNWAGI
PLNAKFFYQLMFAGTAATIVSGAIAERARFVGFFIFSILLTSICYPMVGHWVWGGGFLQKMGFYDFAGSTVVHMVGGWAA
LVGAALIGPRTARYFEGKSYPIPGHNLSIATLGGLILWLGWFGFNGGSTMAADPAAISHIIVITNLAGAAGGVAGTITAW
IYFGKPDLTMTINGMLIGLVSITAGCAFVTSGSAVLIGFVGGIVVVFAVDVFDNFKIDDPVGALSVHMVGGFWGTLAVGL
FSVGPEKFSWYTADSGPAMGLLSGGSFSQVGIQLLGLAIVSGFTVAFSYIVWTALKYLSGIRVTLEDEKLGLDLSEHAME
AYPGFLGDEHL

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:
GO:0008519: Ammonium transporter
GO:0016020: Ammonium transporter
GO:0072488: Ammonium transporter

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=428, Percent_Identity=32.7102803738318, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17550182, Length=449, Percent_Identity=33.630289532294, Blast_Score=211, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI17550180, Length=434, Percent_Identity=32.4884792626728, Blast_Score=207, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI32564042, Length=434, Percent_Identity=31.7972350230415, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI133931100, Length=437, Percent_Identity=32.4942791762014, Blast_Score=200, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324187, Length=431, Percent_Identity=30.6264501160093, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321559, Length=428, Percent_Identity=27.1028037383178, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=26.463700234192, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078314, Length=429, Percent_Identity=39.1608391608392, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 52057; Mature: 51926

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNLKPKPPGQPRHRRASRRWSMLKKPIYLYLTAAILLVWSSAAVAQEAPASVFSGEAK
CCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCHHHHCCCCH
VVADTIWVLFAASLVFFMNAGFAMLETGLCRSKNAVNLLAKNLIVFGLVSLAYWAVGFGI
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
MFGDGNEFMGTTGFFLGGNDNSPAIGEAYDGVFSALNWAGIPLNAKFFYQLMFAGTAATI
EECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VSGAIAERARFVGFFIFSILLTSICYPMVGHWVWGGGFLQKMGFYDFAGSTVVHMVGGWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHCCCCHHHHHHHHHH
ALVGAALIGPRTARYFEGKSYPIPGHNLSIATLGGLILWLGWFGFNGGSTMAADPAAISH
HHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHE
IIVITNLAGAAGGVAGTITAWIYFGKPDLTMTINGMLIGLVSITAGCAFVTSGSAVLIGF
EEEEEECCCCCCCCCEEEEEEEEECCCCEEEEECHHHHHHHHHHHCHHEEECCCCHHHHH
VGGIVVVFAVDVFDNFKIDDPVGALSVHMVGGFWGTLAVGLFSVGPEKFSWYTADSGPAM
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCE
GLLSGGSFSQVGIQLLGLAIVSGFTVAFSYIVWTALKYLSGIRVTLEDEKLGLDLSEHAM
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
EAYPGFLGDEHL
HHCCCCCCCCCC
>Mature Secondary Structure 
SRNLKPKPPGQPRHRRASRRWSMLKKPIYLYLTAAILLVWSSAAVAQEAPASVFSGEAK
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCHHHHCCCCH
VVADTIWVLFAASLVFFMNAGFAMLETGLCRSKNAVNLLAKNLIVFGLVSLAYWAVGFGI
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
MFGDGNEFMGTTGFFLGGNDNSPAIGEAYDGVFSALNWAGIPLNAKFFYQLMFAGTAATI
EECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VSGAIAERARFVGFFIFSILLTSICYPMVGHWVWGGGFLQKMGFYDFAGSTVVHMVGGWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHCCCCHHHHHHHHHH
ALVGAALIGPRTARYFEGKSYPIPGHNLSIATLGGLILWLGWFGFNGGSTMAADPAAISH
HHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHE
IIVITNLAGAAGGVAGTITAWIYFGKPDLTMTINGMLIGLVSITAGCAFVTSGSAVLIGF
EEEEEECCCCCCCCCEEEEEEEEECCCCEEEEECHHHHHHHHHHHCHHEEECCCCHHHHH
VGGIVVVFAVDVFDNFKIDDPVGALSVHMVGGFWGTLAVGLFSVGPEKFSWYTADSGPAM
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCE
GLLSGGSFSQVGIQLLGLAIVSGFTVAFSYIVWTALKYLSGIRVTLEDEKLGLDLSEHAM
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
EAYPGFLGDEHL
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]