Click here to switch to the map view.

The map label for this gene is prsA [H]

Identifier: 158333993

GI number: 158333993

Start: 783580

End: 784593

Strand: Direct

Name: prsA [H]

Synonym: AM1_0807

Alternate gene names: 158333993

Gene position: 783580-784593 (Clockwise)

Preceding gene: 158333992

Following gene: 158333995

Centisome position: 12.05

GC content: 51.38

Gene sequence:

>1014_bases
GTGTTTTGGGGACTCGTAGTGATCCGTCCTGCAACTCTAACTCTGCAACCTCCTCTGCCTCTGGTTGCTGAGAATAATCG
GCTGAAAATCTTCTCCGGTTCTGCCAATGTTTCCCTGGCCCAAGAAGTGGCTCGCTACCTAGGCGTTGATCTGGGCCCCA
TGGTTCGCAAGCGCTTTGCTGATGGCGAACTGTATGTCCAAATTCAAGAATCAATTCGGGGTTGTGATGTCTATTTGATC
CAACCCACCTGCCACCCCGTCAATGACAATTTGATGGAATTGCTGATTATTATTGATGCCTGTCGACGGGCTTCTGCCAG
GCAAATCACGGCAGTGCTGCCTTACTACGGATATTCAAGGGCCGATCGGAAGACAGCCGGTCGAGAATCCATTACGGCCA
AGTTGGTCGCCAACTTAATTACCCAAGCAGGAGCCAGTCGCGTTCTAGCGATGGATTTACACTCCGCTCAGATTCAAGGC
TATTTTGATATTCCGGTGGATCATGTTTATGGGTCTCCTGTACTCTTGGAGTACTTGGCCCGCAAAAAATTGCCTGACCT
AGTGGTTGTTTCCCCTGATGTGGGGGGCGTAGCTAGAGCCCGTGCCTTCGCTAAGAAACTAGATGATGCCCCATTAGCCA
TTATTGATAAACGCCGCCAAGCCCATAATGTCGCCGAAGTGATGAACGTGGTTGGTGATGTCAAAGGGAAAACGGCTGTA
TTAGTGGATGACATGATTGACACGGCAGGCACTATCTCTGCTGGAGCCAAACTACTACGAGAAGAAGGGGCTCGCCAGGT
TTATGCTTGTGCAACCCATGCCATTTTCTCCCCCCCTGCCGTTGAGCGCTTATCTGTAACGGGGCTGTTTGAAGAGGTAT
TGGTGACTAATACCACCCCGATCCCCCCAGAGCGTCATTTCCCCCAACTGCGAGTCTTATCCGTGGCCAGTCTTCTAGGT
GAGACCATCTGGCGAGTCCATGAGGACAGTTCTGTGAGCAGCATGTTCCGCTAG

Upstream 100 bases:

>100_bases
AGTACTTGAGGGATATTAGGATCCCTCGGAGGAATTTAATTAAAACTCACAATTTTCAACCGTACTAAGGGCAATTCTGG
ATGCTAAGATTGCCTAGAAT

Downstream 100 bases:

>100_bases
CCGCTAGATTCGTTTGAATGTTTTGTTTAAGGCTCTTGGTAGAACCGATCCCGATGTTCTGGCAGGGGCCAATCTTCATT
TTCGCCCCCGATCAATAGCT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MFWGLVVIRPATLTLQPPLPLVAENNRLKIFSGSANVSLAQEVARYLGVDLGPMVRKRFADGELYVQIQESIRGCDVYLI
QPTCHPVNDNLMELLIIIDACRRASARQITAVLPYYGYSRADRKTAGRESITAKLVANLITQAGASRVLAMDLHSAQIQG
YFDIPVDHVYGSPVLLEYLARKKLPDLVVVSPDVGGVARARAFAKKLDDAPLAIIDKRRQAHNVAEVMNVVGDVKGKTAV
LVDDMIDTAGTISAGAKLLREEGARQVYACATHAIFSPPAVERLSVTGLFEEVLVTNTTPIPPERHFPQLRVLSVASLLG
ETIWRVHEDSSVSSMFR

Sequences:

>Translated_337_residues
MFWGLVVIRPATLTLQPPLPLVAENNRLKIFSGSANVSLAQEVARYLGVDLGPMVRKRFADGELYVQIQESIRGCDVYLI
QPTCHPVNDNLMELLIIIDACRRASARQITAVLPYYGYSRADRKTAGRESITAKLVANLITQAGASRVLAMDLHSAQIQG
YFDIPVDHVYGSPVLLEYLARKKLPDLVVVSPDVGGVARARAFAKKLDDAPLAIIDKRRQAHNVAEVMNVVGDVKGKTAV
LVDDMIDTAGTISAGAKLLREEGARQVYACATHAIFSPPAVERLSVTGLFEEVLVTNTTPIPPERHFPQLRVLSVASLLG
ETIWRVHEDSSVSSMFR
>Mature_337_residues
MFWGLVVIRPATLTLQPPLPLVAENNRLKIFSGSANVSLAQEVARYLGVDLGPMVRKRFADGELYVQIQESIRGCDVYLI
QPTCHPVNDNLMELLIIIDACRRASARQITAVLPYYGYSRADRKTAGRESITAKLVANLITQAGASRVLAMDLHSAQIQG
YFDIPVDHVYGSPVLLEYLARKKLPDLVVVSPDVGGVARARAFAKKLDDAPLAIIDKRRQAHNVAEVMNVVGDVKGKTAV
LVDDMIDTAGTISAGAKLLREEGARQVYACATHAIFSPPAVERLSVTGLFEEVLVTNTTPIPPERHFPQLRVLSVASLLG
ETIWRVHEDSSVSSMFR

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=46.0063897763578, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=45.859872611465, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI4506129, Length=310, Percent_Identity=46.1290322580645, Blast_Score=284, Evalue=6e-77,
Organism=Homo sapiens, GI84875539, Length=313, Percent_Identity=46.0063897763578, Blast_Score=279, Evalue=2e-75,
Organism=Homo sapiens, GI4506133, Length=347, Percent_Identity=35.1585014409222, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI194018537, Length=341, Percent_Identity=34.8973607038123, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=34.7222222222222, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=34.7222222222222, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=34.7222222222222, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=34.7222222222222, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1787458, Length=311, Percent_Identity=50.8038585209003, Blast_Score=325, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=43.6305732484076, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=43.6305732484076, Blast_Score=264, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=43.6305732484076, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=43.7299035369775, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=31.3609467455621, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319403, Length=315, Percent_Identity=43.4920634920635, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6320946, Length=314, Percent_Identity=42.3566878980892, Blast_Score=242, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6321776, Length=315, Percent_Identity=40.6349206349206, Blast_Score=235, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=42.8571428571429, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=113, Percent_Identity=37.1681415929204, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355239, Length=316, Percent_Identity=45.8860759493671, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI45551540, Length=337, Percent_Identity=43.3234421364985, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=31.830985915493, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=31.830985915493, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=31.830985915493, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=31.830985915493, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24651462, Length=201, Percent_Identity=36.81592039801, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24651464, Length=201, Percent_Identity=36.81592039801, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45552010, Length=201, Percent_Identity=36.81592039801, Blast_Score=131, Evalue=5e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 36878; Mature: 36878

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWGLVVIRPATLTLQPPLPLVAENNRLKIFSGSANVSLAQEVARYLGVDLGPMVRKRFA
CCEEEEEEECCEEEECCCCCEEECCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHCC
DGELYVQIQESIRGCDVYLIQPTCHPVNDNLMELLIIIDACRRASARQITAVLPYYGYSR
CCEEEEEEEHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCC
ADRKTAGRESITAKLVANLITQAGASRVLAMDLHSAQIQGYFDIPVDHVYGSPVLLEYLA
CHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCEEEECCHHHHCCCHHHHHHHH
RKKLPDLVVVSPDVGGVARARAFAKKLDDAPLAIIDKRRQAHNVAEVMNVVGDVKGKTAV
HCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LVDDMIDTAGTISAGAKLLREEGARQVYACATHAIFSPPAVERLSVTGLFEEVLVTNTTP
EEHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
IPPERHFPQLRVLSVASLLGETIWRVHEDSSVSSMFR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MFWGLVVIRPATLTLQPPLPLVAENNRLKIFSGSANVSLAQEVARYLGVDLGPMVRKRFA
CCEEEEEEECCEEEECCCCCEEECCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHCC
DGELYVQIQESIRGCDVYLIQPTCHPVNDNLMELLIIIDACRRASARQITAVLPYYGYSR
CCEEEEEEEHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCC
ADRKTAGRESITAKLVANLITQAGASRVLAMDLHSAQIQGYFDIPVDHVYGSPVLLEYLA
CHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCEEEECCHHHHCCCHHHHHHHH
RKKLPDLVVVSPDVGGVARARAFAKKLDDAPLAIIDKRRQAHNVAEVMNVVGDVKGKTAV
HCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LVDDMIDTAGTISAGAKLLREEGARQVYACATHAIFSPPAVERLSVTGLFEEVLVTNTTP
EEHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
IPPERHFPQLRVLSVASLLGETIWRVHEDSSVSSMFR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7764342 [H]