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The map label for this gene is rfbD [H]

Identifier: 158333988

GI number: 158333988

Start: 778905

End: 779819

Strand: Direct

Name: rfbD [H]

Synonym: AM1_0803

Alternate gene names: 158333988

Gene position: 778905-779819 (Clockwise)

Preceding gene: 158333986

Following gene: 158333990

Centisome position: 11.98

GC content: 51.26

Gene sequence:

>915_bases
ATGGCAAAGTCCCGCATTCTCCTGCTTGGTGCGCAAGGACAATTAGGCCAAGAATTACAAAAAACGTTGCCAACGATGGG
TGAACTGATTGCTGTGGGAAAAGAGACGGTCGATCTGGCTGTCCCTGAACAATTACACGAAGCGATCCTACCCGTTCAGC
CAGACATTATCGTTAATGCCGCAGCTTATACGGCGGTTGACCGGGCTGAGTCAGAGGTTAAATTAGCTCATACAGTAAAT
CAAAAAGCCCCTACAGTCCTAGCCGAACTCGCCCAGACATTAGGTGCGTTACTCATCCATATTTCGACAGACTATGTGTT
TGATGGAACTCAAAGCCATCCTTACACTGAATCCGATGCTCCCCATCCTCAAAGTGTCTATGGCCATTCAAAATGGCAAG
GAGAAGAGGGCATTCGCCAAACCTGGGATCAGCATATTATTTTACGGACGGCCTGGGTTTACGGTACCCAAGGCAAAGGT
AATTTTGTGAAAACGATGTTGCGACTCGGCAGGGAGCGATCAGACGTTCGGGTTGTGGATGACCAAGTTGGCACGCCCAC
ATGGGCCAAGGAGATTGGAGACGCGATCGCATCCCTGATTCACCACTGGTCCACCTCGGATCCGGTCACCCAAGCAGAAC
TGTATGGCACCTATCATTTCACCAACCGTGGAGTCGCCAGCTGGTATGATTTCGCCGTTGCCATCTTTGCTGAAGCCCAA
GCTCTGGGTGTTCCCCTTCAAATCGAACGGGTCATCCCCATTACCACGCCGGAATATCCGCTACCGGCCCCAAGGCCAGC
CTATTCTGTCTTGAGCAATTGCAAAATTACCCCCATCCTGGGGCAACCTGCCCCCCATTGGCGACAGTCTTTACGCCAGA
TGTTGCAGGAGCTAATTCAAGACGCCTCCATATAA

Upstream 100 bases:

>100_bases
CGTAGTGATTTAGATCTGTATCTTCTTCTTTGAAGTGGCTAATAGATATCTACTCTTTGAACAAGATAGGATCCGTGTTA
GTGCACGATAGGAATTCCCC

Downstream 100 bases:

>100_bases
TGGAGCCACTTCAGGGTAGCCGTTATAAATTAACGTGGGCTGTCGTTCCCATACGGCTGGATGCACCCCAGTGCCAATCA
AACGCTCCTCCGCTTGAGAC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MAKSRILLLGAQGQLGQELQKTLPTMGELIAVGKETVDLAVPEQLHEAILPVQPDIIVNAAAYTAVDRAESEVKLAHTVN
QKAPTVLAELAQTLGALLIHISTDYVFDGTQSHPYTESDAPHPQSVYGHSKWQGEEGIRQTWDQHIILRTAWVYGTQGKG
NFVKTMLRLGRERSDVRVVDDQVGTPTWAKEIGDAIASLIHHWSTSDPVTQAELYGTYHFTNRGVASWYDFAVAIFAEAQ
ALGVPLQIERVIPITTPEYPLPAPRPAYSVLSNCKITPILGQPAPHWRQSLRQMLQELIQDASI

Sequences:

>Translated_304_residues
MAKSRILLLGAQGQLGQELQKTLPTMGELIAVGKETVDLAVPEQLHEAILPVQPDIIVNAAAYTAVDRAESEVKLAHTVN
QKAPTVLAELAQTLGALLIHISTDYVFDGTQSHPYTESDAPHPQSVYGHSKWQGEEGIRQTWDQHIILRTAWVYGTQGKG
NFVKTMLRLGRERSDVRVVDDQVGTPTWAKEIGDAIASLIHHWSTSDPVTQAELYGTYHFTNRGVASWYDFAVAIFAEAQ
ALGVPLQIERVIPITTPEYPLPAPRPAYSVLSNCKITPILGQPAPHWRQSLRQMLQELIQDASI
>Mature_303_residues
AKSRILLLGAQGQLGQELQKTLPTMGELIAVGKETVDLAVPEQLHEAILPVQPDIIVNAAAYTAVDRAESEVKLAHTVNQ
KAPTVLAELAQTLGALLIHISTDYVFDGTQSHPYTESDAPHPQSVYGHSKWQGEEGIRQTWDQHIILRTAWVYGTQGKGN
FVKTMLRLGRERSDVRVVDDQVGTPTWAKEIGDAIASLIHHWSTSDPVTQAELYGTYHFTNRGVASWYDFAVAIFAEAQA
LGVPLQIERVIPITTPEYPLPAPRPAYSVLSNCKITPILGQPAPHWRQSLRQMLQELIQDASI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=310, Percent_Identity=30.6451612903226, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI33519455, Length=310, Percent_Identity=30.6451612903226, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1788352, Length=304, Percent_Identity=39.8026315789474, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 33534; Mature: 33403

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSRILLLGAQGQLGQELQKTLPTMGELIAVGKETVDLAVPEQLHEAILPVQPDIIVNA
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCCEEEH
AAYTAVDRAESEVKLAHTVNQKAPTVLAELAQTLGALLIHISTDYVFDGTQSHPYTESDA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCEEECCCCCCCCCCCCC
PHPQSVYGHSKWQGEEGIRQTWDQHIILRTAWVYGTQGKGNFVKTMLRLGRERSDVRVVD
CCCHHHCCCCCCCCCHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEC
DQVGTPTWAKEIGDAIASLIHHWSTSDPVTQAELYGTYHFTNRGVASWYDFAVAIFAEAQ
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEECCCCHHHHHHHHHHHHHHHH
ALGVPLQIERVIPITTPEYPLPAPRPAYSVLSNCKITPILGQPAPHWRQSLRQMLQELIQ
HHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHH
DASI
HCCC
>Mature Secondary Structure 
AKSRILLLGAQGQLGQELQKTLPTMGELIAVGKETVDLAVPEQLHEAILPVQPDIIVNA
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCCEEEH
AAYTAVDRAESEVKLAHTVNQKAPTVLAELAQTLGALLIHISTDYVFDGTQSHPYTESDA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCEEECCCCCCCCCCCCC
PHPQSVYGHSKWQGEEGIRQTWDQHIILRTAWVYGTQGKGNFVKTMLRLGRERSDVRVVD
CCCHHHCCCCCCCCCHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEC
DQVGTPTWAKEIGDAIASLIHHWSTSDPVTQAELYGTYHFTNRGVASWYDFAVAIFAEAQ
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEECCCCHHHHHHHHHHHHHHHH
ALGVPLQIERVIPITTPEYPLPAPRPAYSVLSNCKITPILGQPAPHWRQSLRQMLQELIQ
HHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHH
DASI
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]