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The map label for this gene is nfi [H]

Identifier: 158333970

GI number: 158333970

Start: 762165

End: 762854

Strand: Direct

Name: nfi [H]

Synonym: AM1_0784

Alternate gene names: 158333970

Gene position: 762165-762854 (Clockwise)

Preceding gene: 158333969

Following gene: 158333971

Centisome position: 11.72

GC content: 50.87

Gene sequence:

>690_bases
GTGCAAGTTCCTAAGATTGAGAATTGGCCCCAAACGGCTCAAGACGCTATTCAACTCCAAAAACAACTTTGTTCCCAGGT
CATCCCCACCGACGACTTTAATGAAGTGCGCTTTGTCGCGGGAGTGGATGTAGGTTTTGAAAACCAAGGTAAAACGACTC
GCGCTGCGATCGCAGTTCTGACTTGGCCTGAGCTAAAACTGCACGAAACGACGATTGCTCGCCAACCCACTAAGTTTCCC
TACATTCCAGGCTTGCTATCGTTTCGAGAAATTCCAACGGTTTTAGCAGCTTTAGCCCAAATTCAAACCACCCCAGATCT
CCTGCTTTGTGATGGTCAGGGGCTTGCCCATCCCCGACGTTTTGGGATTGCCTGCCATTTAGGCGTGCTGGCGGATCTAC
CGGCGATTGGTGTAGCCAAATCACGTTTGGTGGGAAAGCACGAGCCTGTAGATCAAACCCGAGGGAATTGGCAGCCTCTA
ATCGATAAGGACGAACGGATTGGGGCAGTCCTCCGCACTCGCCCTAATACAAAGCCCCTGTACATTTCTTTGGGACATCG
AATTAGTTTAGACAGTGCCATTGAGTATGTAATGAGCTGTACCACCCGCTATCGGCTACCGGAGACTACCCGTTGGGCCG
ACAAGTTAGCGTCTTCCAAAATCCCCCCCAACCCTGAAAAGAGGAGTTGA

Upstream 100 bases:

>100_bases
TGCTAACCCTTTACTTTTCATTCTGGGAATCATGGGCGGTATGCTCGGAGCCCTGATGGTGATGGACTTTATTGATACCC
ACTGGCTTCATAAGTGGTAA

Downstream 100 bases:

>100_bases
AATCTCCGAGCTGAGCGAAGTCGTAGTCTTTGACTCCAGGTGTATTCCAGATTTAAGCAACGTTTGTCAGAATTAATGTC
CAGGCATCAGAATCCTGCAA

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MQVPKIENWPQTAQDAIQLQKQLCSQVIPTDDFNEVRFVAGVDVGFENQGKTTRAAIAVLTWPELKLHETTIARQPTKFP
YIPGLLSFREIPTVLAALAQIQTTPDLLLCDGQGLAHPRRFGIACHLGVLADLPAIGVAKSRLVGKHEPVDQTRGNWQPL
IDKDERIGAVLRTRPNTKPLYISLGHRISLDSAIEYVMSCTTRYRLPETTRWADKLASSKIPPNPEKRS

Sequences:

>Translated_229_residues
MQVPKIENWPQTAQDAIQLQKQLCSQVIPTDDFNEVRFVAGVDVGFENQGKTTRAAIAVLTWPELKLHETTIARQPTKFP
YIPGLLSFREIPTVLAALAQIQTTPDLLLCDGQGLAHPRRFGIACHLGVLADLPAIGVAKSRLVGKHEPVDQTRGNWQPL
IDKDERIGAVLRTRPNTKPLYISLGHRISLDSAIEYVMSCTTRYRLPETTRWADKLASSKIPPNPEKRS
>Mature_229_residues
MQVPKIENWPQTAQDAIQLQKQLCSQVIPTDDFNEVRFVAGVDVGFENQGKTTRAAIAVLTWPELKLHETTIARQPTKFP
YIPGLLSFREIPTVLAALAQIQTTPDLLLCDGQGLAHPRRFGIACHLGVLADLPAIGVAKSRLVGKHEPVDQTRGNWQPL
IDKDERIGAVLRTRPNTKPLYISLGHRISLDSAIEYVMSCTTRYRLPETTRWADKLASSKIPPNPEKRS

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=220, Percent_Identity=38.6363636363636, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI257467548, Length=215, Percent_Identity=34.4186046511628, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI257467550, Length=203, Percent_Identity=34.4827586206897, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI87082357, Length=203, Percent_Identity=49.7536945812808, Blast_Score=208, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI71981509, Length=136, Percent_Identity=32.3529411764706, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 25519; Mature: 25519

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVPKIENWPQTAQDAIQLQKQLCSQVIPTDDFNEVRFVAGVDVGFENQGKTTRAAIAVL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEE
TWPELKLHETTIARQPTKFPYIPGLLSFREIPTVLAALAQIQTTPDLLLCDGQGLAHPRR
ECCCCEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHH
FGIACHLGVLADLPAIGVAKSRLVGKHEPVDQTRGNWQPLIDKDERIGAVLRTRPNTKPL
HHHEEHHHHHHCCCCHHHHHHHHCCCCCCCHHCCCCCCCCCCCCHHHHHHHHCCCCCCCE
YISLGHRISLDSAIEYVMSCTTRYRLPETTRWADKLASSKIPPNPEKRS
EEECCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQVPKIENWPQTAQDAIQLQKQLCSQVIPTDDFNEVRFVAGVDVGFENQGKTTRAAIAVL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEE
TWPELKLHETTIARQPTKFPYIPGLLSFREIPTVLAALAQIQTTPDLLLCDGQGLAHPRR
ECCCCEEHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHH
FGIACHLGVLADLPAIGVAKSRLVGKHEPVDQTRGNWQPLIDKDERIGAVLRTRPNTKPL
HHHEEHHHHHHCCCCHHHHHHHHCCCCCCCHHCCCCCCCCCCCCHHHHHHHHCCCCCCCE
YISLGHRISLDSAIEYVMSCTTRYRLPETTRWADKLASSKIPPNPEKRS
EEECCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA