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The map label for this gene is purT [H]

Identifier: 158333955

GI number: 158333955

Start: 745771

End: 746997

Strand: Reverse

Name: purT [H]

Synonym: AM1_0769

Alternate gene names: 158333955

Gene position: 746997-745771 (Counterclockwise)

Preceding gene: 158333958

Following gene: 158333954

Centisome position: 11.49

GC content: 54.44

Gene sequence:

>1227_bases
ATGATCAGCCGTCCTGCCAACACTTCTCTCGCAGCACCTTGGGGTGCCCCTTTTTCTGATTCAGCCAAGCGGCTGCTGCT
GTTGGGGTCAGGAGAACTAGGTCGGGAAGTGGCCCTAGAGGCCATGCGTTTGGGGATTGAAGTGATTGCATCGGATGCCT
ACGATCATGCCCCTGCAATGCAGTTTGCCCATCGGAATGTGGTGATCAATATGCAGGATGGGGCGGCCCTGCGTCAGGTC
GTAGAGTCCGTGAAACCGGATCTGATTGTGCCAGAGATTGAAGCTATTGCCACTGCAACGCTGCTCGACCTAGAAGCGGA
AGGATGGAACGTAATTCCCACAGCCAAAGCCACTAATCTGACCATGGATCGGGAAGGCATTCGGCGTCTGGCAGCTAGTG
ATTTAGGTCTAAAAACCTCCCCCTTTCAGTTTGCATCTACTCAAGCCGAATATGAGCAGGCGGTGCAGAAAGTGGGTCTT
CCCTGCGTGGTCAAACCGGTCATGAGTTCTTCAGGCAAAGGCCAAAGCTTAGTCAAAACAGACAGTGAAATTATCCCAGC
CTGGACCTATGCTCTAGAAGCCGGACGTGGCAATGCTCCTCGCGTTATCGTTGAAGGCTTTGTCGAATTCGAAACAGAAA
TTACCTTACTCACCGTGCGGTCCCAGTCGGGCACCCACTTCTGCTCCCCCATTGGCCATGTCCAGCAAAGCGGGGACTAT
CGAGAGTCCTGGCAACCCTGCCCTCTCCATCCTGATACGCTGCAAGCCTGTCAAGATATGGCCCGCCAAATTACCGATGC
CCTGGGGGGCTGGGGATTGTTTGGAGTGGAACTGTTTATCGCTGGCCCTGCCGATCAGGCCCAGACAGTGTATTTCAGCG
AAGTCAGTCCTCGCCCCCACGACACAGGCATGGTCACCATGGTCAGCCAAAACATGTCAGAGTTTGAGCTACATGCTCGC
GCCATTCTGGGACTCCCCATTGGCGATATCCAGCTGGTTAGCCCCGGAGCGTCTGCAGTCATTTTGGCCTCAGACGCATC
TAAGGAACCCAAGATTGAAGGTGTTGCTGCGGCTCTTACGGTGCCAACCAGTCGCGTGCGCCTATTTGGCAAACCCACTG
CCCATAAAAATCGACGGATGGGGGTGGCCCTAGCCCTCGGTGAGACAATAGAAGAAGCCAGACAACGAGCTAAAACCTGT
GCCCAGCAAGTTACCGTTACTAGTTAA

Upstream 100 bases:

>100_bases
TTCTATATCCCACAAACTGCTAATCAGCATTATGGTATGAAACCGTTATGGGTCATACTGCTTCCATGTAAATCTATAGA
ATGAATCCTCCCTGACAACG

Downstream 100 bases:

>100_bases
TCAGCGCGAAAACTTATTCTGATTACCTACCCGTTTTTACCTGCATTTGACTATAGACAAAGACCATAGGCAGCTATAAA
AATCGTCAAGAGTTAGATGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MISRPANTSLAAPWGAPFSDSAKRLLLLGSGELGREVALEAMRLGIEVIASDAYDHAPAMQFAHRNVVINMQDGAALRQV
VESVKPDLIVPEIEAIATATLLDLEAEGWNVIPTAKATNLTMDREGIRRLAASDLGLKTSPFQFASTQAEYEQAVQKVGL
PCVVKPVMSSSGKGQSLVKTDSEIIPAWTYALEAGRGNAPRVIVEGFVEFETEITLLTVRSQSGTHFCSPIGHVQQSGDY
RESWQPCPLHPDTLQACQDMARQITDALGGWGLFGVELFIAGPADQAQTVYFSEVSPRPHDTGMVTMVSQNMSEFELHAR
AILGLPIGDIQLVSPGASAVILASDASKEPKIEGVAAALTVPTSRVRLFGKPTAHKNRRMGVALALGETIEEARQRAKTC
AQQVTVTS

Sequences:

>Translated_408_residues
MISRPANTSLAAPWGAPFSDSAKRLLLLGSGELGREVALEAMRLGIEVIASDAYDHAPAMQFAHRNVVINMQDGAALRQV
VESVKPDLIVPEIEAIATATLLDLEAEGWNVIPTAKATNLTMDREGIRRLAASDLGLKTSPFQFASTQAEYEQAVQKVGL
PCVVKPVMSSSGKGQSLVKTDSEIIPAWTYALEAGRGNAPRVIVEGFVEFETEITLLTVRSQSGTHFCSPIGHVQQSGDY
RESWQPCPLHPDTLQACQDMARQITDALGGWGLFGVELFIAGPADQAQTVYFSEVSPRPHDTGMVTMVSQNMSEFELHAR
AILGLPIGDIQLVSPGASAVILASDASKEPKIEGVAAALTVPTSRVRLFGKPTAHKNRRMGVALALGETIEEARQRAKTC
AQQVTVTS
>Mature_408_residues
MISRPANTSLAAPWGAPFSDSAKRLLLLGSGELGREVALEAMRLGIEVIASDAYDHAPAMQFAHRNVVINMQDGAALRQV
VESVKPDLIVPEIEAIATATLLDLEAEGWNVIPTAKATNLTMDREGIRRLAASDLGLKTSPFQFASTQAEYEQAVQKVGL
PCVVKPVMSSSGKGQSLVKTDSEIIPAWTYALEAGRGNAPRVIVEGFVEFETEITLLTVRSQSGTHFCSPIGHVQQSGDY
RESWQPCPLHPDTLQACQDMARQITDALGGWGLFGVELFIAGPADQAQTVYFSEVSPRPHDTGMVTMVSQNMSEFELHAR
AILGLPIGDIQLVSPGASAVILASDASKEPKIEGVAAALTVPTSRVRLFGKPTAHKNRRMGVALALGETIEEARQRAKTC
AQQVTVTS

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=56.3775510204082, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1786733, Length=218, Percent_Identity=26.605504587156, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=382, Percent_Identity=27.2251308900524, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43722; Mature: 43722

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50975 ATP_GRASP ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRPANTSLAAPWGAPFSDSAKRLLLLGSGELGREVALEAMRLGIEVIASDAYDHAPAM
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCHHH
QFAHRNVVINMQDGAALRQVVESVKPDLIVPEIEAIATATLLDLEAEGWNVIPTAKATNL
HHCCCCEEEEECCCHHHHHHHHHCCCCEECCCHHHHHHHHEEEEECCCCEEECCCCCCCE
TMDREGIRRLAASDLGLKTSPFQFASTQAEYEQAVQKVGLPCVVKPVMSSSGKGQSLVKT
EECHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEECC
DSEIIPAWTYALEAGRGNAPRVIVEGFVEFETEITLLTVRSQSGTHFCSPIGHVQQSGDY
CCCCCCCEEEEEECCCCCCCEEEEEHHHEECEEEEEEEEECCCCCCHHCCHHHHHCCCCH
RESWQPCPLHPDTLQACQDMARQITDALGGWGLFGVELFIAGPADQAQTVYFSEVSPRPH
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCC
DTGMVTMVSQNMSEFELHARAILGLPIGDIQLVSPGASAVILASDASKEPKIEGVAAALT
CCCEEEEECCCCHHHHHHHHHEECCCCCCEEEECCCCCEEEEECCCCCCCCCCCEEEEEE
VPTSRVRLFGKPTAHKNRRMGVALALGETIEEARQRAKTCAQQVTVTS
CCHHHEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MISRPANTSLAAPWGAPFSDSAKRLLLLGSGELGREVALEAMRLGIEVIASDAYDHAPAM
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCCCHHH
QFAHRNVVINMQDGAALRQVVESVKPDLIVPEIEAIATATLLDLEAEGWNVIPTAKATNL
HHCCCCEEEEECCCHHHHHHHHHCCCCEECCCHHHHHHHHEEEEECCCCEEECCCCCCCE
TMDREGIRRLAASDLGLKTSPFQFASTQAEYEQAVQKVGLPCVVKPVMSSSGKGQSLVKT
EECHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEECC
DSEIIPAWTYALEAGRGNAPRVIVEGFVEFETEITLLTVRSQSGTHFCSPIGHVQQSGDY
CCCCCCCEEEEEECCCCCCCEEEEEHHHEECEEEEEEEEECCCCCCHHCCHHHHHCCCCH
RESWQPCPLHPDTLQACQDMARQITDALGGWGLFGVELFIAGPADQAQTVYFSEVSPRPH
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCC
DTGMVTMVSQNMSEFELHARAILGLPIGDIQLVSPGASAVILASDASKEPKIEGVAAALT
CCCEEEEECCCCHHHHHHHHHEECCCCCCEEEECCCCCEEEEECCCCCCCCCCCEEEEEE
VPTSRVRLFGKPTAHKNRRMGVALALGETIEEARQRAKTCAQQVTVTS
CCHHHEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA