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The map label for this gene is lasT [H]

Identifier: 158333940

GI number: 158333940

Start: 734836

End: 735567

Strand: Direct

Name: lasT [H]

Synonym: AM1_0754

Alternate gene names: 158333940

Gene position: 734836-735567 (Clockwise)

Preceding gene: 158333939

Following gene: 158333941

Centisome position: 11.3

GC content: 50.82

Gene sequence:

>732_bases
ATGCAGTTTTCATTCATCCTGGTTGAATCAGAAGTTCCAGAGAACCTCGGGGCTGCCGCCCGAGCTTTGAATACCATGGG
CCATACGGATTTGCGGCTAGTGCGACCCCGATGCGATCGCAACTCTGAAAAAGCCCAAGTCCTGGCCCATGGTTCCCAAG
CTGTCTTGAGTGCAGCTCCCATTTATCAGAATTTATCCGACGCGCTGCATGACATTGATTTTGCCTGCGCCACTACCGCC
CGCCATCGCTATGAGAAGTATCAGTATATTTCTGTCCGGGAGGTGCCCCAACGCTTACAGGCAAAGGGAGAGACTCTGCA
GCAGGTTGCTTTTGTGTTTGGGGGAGAACGGTCGGGATTGAATCGACACGATATCGATTGCTGCGATTTGATCACGACCA
TCCCTCAAGGGAATCCCTATCCGTCCTTGAACTTAGCTCAGGCAGTGATGGTGTACAGCTTTATGCTGTCTGAGTCGGCG
GTACATATTAAAGACCAGCGACTGAATCGTACCCCCATGCCCCCGCTAGAATATGCCAATCTCAAACACTCTTCTCTTCA
GCTTATGGAGCGCATCGGCTTGGCCGAACGCTATCAGAAGTACGTTACTCGCGGTTTAGCCCGATTGGGAACTGAAGATC
TATATTTATTGCATAATATTCGAGCCTGTATTGATCGCACCTTAGATCACCTGGAAGCCAAGCAGGGGAATCATCACCAT
GCCGATTCCTGA

Upstream 100 bases:

>100_bases
TAATGCCTGGGTAGATCGTGCTAGCGTCAATAGGCAGAGAGTATCCAGTATTAGGATTAATGCCGTGTACTCCTTGTGAC
AAGGCAAACGTTCTATCTTC

Downstream 100 bases:

>100_bases
TCCCTTTGCCCACTTGGATACAGTATCTTTAACCCCGATTGCCTACATTCGATCTTGTTACCCTGAACGATTTGGCATTC
CCCGACAGGCAGGCTTGGTG

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MQFSFILVESEVPENLGAAARALNTMGHTDLRLVRPRCDRNSEKAQVLAHGSQAVLSAAPIYQNLSDALHDIDFACATTA
RHRYEKYQYISVREVPQRLQAKGETLQQVAFVFGGERSGLNRHDIDCCDLITTIPQGNPYPSLNLAQAVMVYSFMLSESA
VHIKDQRLNRTPMPPLEYANLKHSSLQLMERIGLAERYQKYVTRGLARLGTEDLYLLHNIRACIDRTLDHLEAKQGNHHH
ADS

Sequences:

>Translated_243_residues
MQFSFILVESEVPENLGAAARALNTMGHTDLRLVRPRCDRNSEKAQVLAHGSQAVLSAAPIYQNLSDALHDIDFACATTA
RHRYEKYQYISVREVPQRLQAKGETLQQVAFVFGGERSGLNRHDIDCCDLITTIPQGNPYPSLNLAQAVMVYSFMLSESA
VHIKDQRLNRTPMPPLEYANLKHSSLQLMERIGLAERYQKYVTRGLARLGTEDLYLLHNIRACIDRTLDHLEAKQGNHHH
ADS
>Mature_243_residues
MQFSFILVESEVPENLGAAARALNTMGHTDLRLVRPRCDRNSEKAQVLAHGSQAVLSAAPIYQNLSDALHDIDFACATTA
RHRYEKYQYISVREVPQRLQAKGETLQQVAFVFGGERSGLNRHDIDCCDLITTIPQGNPYPSLNLAQAVMVYSFMLSESA
VHIKDQRLNRTPMPPLEYANLKHSSLQLMERIGLAERYQKYVTRGLARLGTEDLYLLHNIRACIDRTLDHLEAKQGNHHH
ADS

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=157, Percent_Identity=40.7643312101911, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788881, Length=150, Percent_Identity=36, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27423; Mature: 27423

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSFILVESEVPENLGAAARALNTMGHTDLRLVRPRCDRNSEKAQVLAHGSQAVLSAAP
CCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHCCCHHHHHHHH
IYQNLSDALHDIDFACATTARHRYEKYQYISVREVPQRLQAKGETLQQVAFVFGGERSGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NRHDIDCCDLITTIPQGNPYPSLNLAQAVMVYSFMLSESAVHIKDQRLNRTPMPPLEYAN
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHCC
LKHSSLQLMERIGLAERYQKYVTRGLARLGTEDLYLLHNIRACIDRTLDHLEAKQGNHHH
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ADS
CCC
>Mature Secondary Structure
MQFSFILVESEVPENLGAAARALNTMGHTDLRLVRPRCDRNSEKAQVLAHGSQAVLSAAP
CCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHCCCHHHHHHHH
IYQNLSDALHDIDFACATTARHRYEKYQYISVREVPQRLQAKGETLQQVAFVFGGERSGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NRHDIDCCDLITTIPQGNPYPSLNLAQAVMVYSFMLSESAVHIKDQRLNRTPMPPLEYAN
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHCC
LKHSSLQLMERIGLAERYQKYVTRGLARLGTEDLYLLHNIRACIDRTLDHLEAKQGNHHH
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ADS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]